Article
Ecology
Vincent Somerville, Helene Berthoud, Remo S. Schmidt, Hans-Peter Bachmann, Yi Helene Meng, Pascal Fuchsmann, Ueli von Ah, Philipp Engel
Summary: Undefined starter cultures are bacterial communities used in cheese making that have evolved through domestication and are phenotypically stable, with functionally redundant strains coexisting but exhibiting significant differences in phage resistance potential. The presence of functionally equivalent strains and stable coexistence of phages in the community highlight the importance of bacteria-phage interactions that differ from traditional kill-the-winner dynamics.
Article
Food Science & Technology
Peng Yu, Yu Zhao, Yang Jiang, Yu Yang, Xiaoming Liu, Heping Zhang, Jianxin Zhao, Yuan -kun Lee, Hao Zhang, Wei Chen
Summary: Few studies have focused on the capacity of lactic acid bacteria in utilizing soybean carbohydrates. This study reveals that Leuconostoc mesenteroides, Lactococcus lactis, and Streptococcus thermophilus can utilize sucrose and raffinose-family oligosaccharides in soybeans, providing insights for suitable strain selection in soybean fermentation.
Article
Biotechnology & Applied Microbiology
Fan Wu, Xinmei Xie, Tao Du, Xiaodan Jiang, Wei Miao, Tiancheng Wang
Summary: Lactococcus lactis is recognized as a safe microorganism that regulates the intestinal micro-ecological balance and improves the immune performance of animals. However, pathogenic Lactococcus lactis has been increasingly isolated from clinical cases, causing issues in dairy farming where it is used as a probiotic. This review aims to analyze the serious clinical mastitis caused by L. lactis and discuss its characteristics and diversity in the context of probiotic use.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhikun Yang, Chuang Li, Tao Wang, Zhihua Li, Xiaobo Zou, Xiaowei Huang, Xiaodong Zhai, Jiyong Shi, Tingting Shen, Yunyun Gong, Melvin Holmes, Megan Povey
Summary: A novel probiotic film made of gellan gum, cranberry extract, and Lactococcus lactis was successfully fabricated. The addition of Lactococcus lactis significantly enhanced the antibacterial activity of the film. Cranberry extract improved the antioxidant activity and survivability of Lactococcus lactis in the film. The combination of 2.0% Lactococcus lactis and 0.5% cranberry extract resulted in the best comprehensive properties of the film, which also showed optimal preservation effect on fresh-cut potatoes and apples. Therefore, the GN/2.0% LA/0.5% CE probiotic film is suitable for fruit and vegetable preservation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Giovanna A. Belo, Barbara F. Cordeiro, Emiliano R. Oliveira, Marina P. Braga, Sara H. da Silva, Bruno G. Costa, Flaviano dos S. Martins, Gwenael Jan, Yves Le Loir, Alfonso Gala-Garcia, Enio Ferreira, Vasco Azevedo, Fillipe L. R. do Carmo
Summary: The study showed that the SlpB protein can enhance the probiotic potential of L. lactis strain to alleviate DSS-induced colitis in mice.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Microbiology
Chunyan Tan, Qiuyue Li, Xuejiao Yang, Jiayu Chen, Qilin Zhang, Xianyu Deng
Summary: Lactococcus lactis KUST48 (LLK48) with antibacterial effect against Streptococcus agalactiae was isolated and used as a potential probiotic to treat zebrafish infected with S. agalactiae. The study demonstrated the positive therapeutic effects of LLK48 on the intestinal microbiota and physiological functions of zebrafish infected with S. agalactiae, providing a new approach to treat S. agalactiae infections in fish aquaculture.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Cansu Altin, Tijjani Ismail Kabwanga, Fadime Kiran, Sebnem Ozturkoglu-Budak
Summary: Lactococcus lactis subsp. lactis NCCB100539 strain isolated from artisanal raw ewe's milk cheese showed potential as a starter culture for white-brined cheese. The strain was evaluated for safety and antibiotic sensitivity, and cheeses produced with the strain exhibited improved proteolysis, sensory scores, and microbial quality. Neither live nor dead cells and CFE of the strain showed cytotoxicity. Therefore, L. lactis NCCB100539 could be a suitable starter candidate for industrial white-brined cheeses.
FOOD SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Liang Li, Xiaoyu Yang, Rui Hong, Fei Liu
Summary: The gene and protein expressions of L. lactis were significantly affected by different culture conditions, with fructose and pH having a significant impact on sugar metabolism.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Monika Pytka, Monika Kordowska-Wiater, Justyna Wajs, Pawel Glibowski, Ewa Sajnaga
Summary: The aim of this study was to isolate and identify Lactococcus lactis strains from Polish fermented cow milk, and verify their probiotic properties. Two strains, A13 and A14, were found to be the most tolerant to high NaCl concentrations and bile salts, and showed strong acidification of milk. They also exhibited activity against pathogenic strains. These selected strains have potential as industrial starter cultures for producing cottage cheese with good sensory and rheological properties.
APPLIED SCIENCES-BASEL
(2022)
Article
Microbiology
Sofia Markakiou, Ana Rute Neves, Ahmad A. Zeidan, Paula Gaspar
Summary: Lactococcus lactis and Streptococcus thermophilus are important lactic acid bacteria used globally in dairy fermentations. They are being explored as hosts for heterologous protein production. The tetracycline-inducible expression system was implemented in both bacteria and optimization of the repression level was found to be necessary for efficient induction.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ilavenil Soundharrajan, Yong Hee Yoon, Karnan Muthusamy, Jeong-Sung Jung, Hyun Jeong Lee, Ouk-Kyu Han, Ki Choon Choi
Summary: The isolated potent lactic acid bacteria showed significant antagonistic activities against intestinal pathogens and exhibited probiotic characteristics, indicating them as a potential alternative strategy for antibiotics to control infectious pathogens.
Article
Biotechnology & Applied Microbiology
Nathalie N. S. E. Henriksen, Mads Frederik Hansen, Heiko T. Kiesewalter, Jakob Russel, Joseph Nesme, Kevin R. Foster, Birte Svensson, Gunnar Oregaard, Jakob Herschend, Mette Burmolle
Summary: This study shows that cultivation in a structured environment facilitates coexistence, evolution, and adaptation of bacterial communities. Co-cultivation in a biofilm model resulted in stable coexistence of two bacterial species and drove the evolution of high-yield variants. This study emphasizes the importance of structured environments for the cultivation and evolution of industrially important bacterial communities.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Food Science & Technology
Wenping Lei, Lulu Hao, Shanbing You, Hui Yao, Chengguo Liu, Hui Zhou
Summary: Lactococcus lactis C15 exhibits probiotic properties and produces a bacteriocin with strong inhibitory activity against pathogens, making it a potential alternative for food preservation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Shadi Pakroo, Gloria Ghion, Armin Tarrah, Alessio Giacomini, Viviana Corich
Summary: The study found that microcapsules prepared with 2'-fucosyllactose and inulin were most effective in protecting Streptococcus thermophilus strain TH982 under simulated gastrointestinal conditions, with a significant release of cells within a short period of time.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Zhiyuan Xu, Qingbin Guo, Hui Zhang, Zhiqiang Xiong, Xiumin Zhang, Lianzhong Ai
Summary: The application of Streptococcus thermophilus S-3 in yogurt production led to the generation of EPS-S3 with high apparent viscosity and quality, when co-cultured with Lactobacillus delbrueckii, it significantly increased the acidifying rate and improved the rheological properties compared to using Lactobacillus bulgaricus only. The production mechanism of EPS-S3 was discussed for potential applications in milk production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Multidisciplinary
Gabriela N. Tenea, Daniela Olmedo, Clara Ortega
Article
Chemistry, Multidisciplinary
Gabriela N. Tenea, Daniela Olmedo
Summary: The study evaluated the effect of specific peptide extract combinations from two probiotic bacteria and commercial food additive nisin on artificially inoculated mango wedges, showing that these antimicrobial cocktails were effective in reducing bacterial counts, with particular efficiency in inhibiting Staphylococcus aureus and Acinetobacter baumannii.
APPLIED SCIENCES-BASEL
(2021)
Article
Infectious Diseases
Gabriela N. Tenea, Clara Ortega
Summary: The genome characterization of Lactiplantibacillus plantarum strain UTNGt2 isolated from wild copoazu or white cacao has been described, with information on genome size, GC content, gene number, and their functions.
Article
Microbiology
Gabriela N. Tenea, Pamela Hurtado
Summary: The genome of Weissella cibaria UTNGt21O strain harbors genes related to carbohydrate metabolism, cellular process, stress responses, cofactors, and vitamins, conferring probiotic features. It exhibits high genetic diversity and potential antimicrobial properties, while also demonstrating safety.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Gabriela N. Tenea, Fabricio Veintimilla
Summary: The study focused on the production of bioethanol, specifically looking at the efficiency of native yeasts in fermenting black sugarcane juice. Results showed that the Lev6 and Lev30 strains were highly efficient in biomass and ethanol production, with Lev6 exhibiting the highest ethanol concentration and volumetric productivity. Gas chromatography coupled to mass spectrometry indicated the presence of ethanol and therapeutic substances in all samples, showing greater efficiency than commercial controls in alcoholic fermentation of black sugarcane juice.
Article
Infectious Diseases
Gabriela N. Tenea, Evelyn Angamarca, Daniela Olmedo
Summary: This study evaluates the antimicrobial potential of peptide-protein extracts from three native probiotic strains and their combination with EDTA against multidrug-resistant Staphylococcus aureus and Citrobacter freundii. The results show that two of the tested extracts exhibited a greater inhibitory effect on both pathogens. Transmission electron microscopy and scanning electronic microscopy analysis revealed structural membrane damage and cell death induced by the extracts.
Article
Microbiology
Gabriela N. Tenea, Pamela Ascanta
Summary: This study describes the genome sequencing and characterization of a novel strain of Lactiplantibacillus plantarum isolated from wild Solanum quitoense fruits. The genome analysis identified various biosynthetic gene clusters and metabolic compounds. The strain's genome contains a large number of protein-coding genes and lacks acquired resistance genes and virulence factors, suggesting its safety. Further analysis revealed potential antimicrobial properties and the presence of unique gene clusters, indicating its potential use as an antimicrobial producer or probiotic strain.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Gabriela N. Tenea
Summary: This study utilizes whole-genome resequencing to analyze the genetic variations and annotations of two native probiotic strains. The results show that these strains exhibit differences in their adaptability to stressors and antimicrobial strength. Furthermore, the analysis reveals variations in the antimicrobial gene cluster organization and genomic islands between the strains, indicating high genome variation.
Article
Microbiology
Gabriela N. Tenea, Gabriela Lizeth Gonzalez, Jose Luis Moreno
Summary: A strain of Bacillus subtilis Fa17.2 isolated from a plant inflorescence in Ecuador showed potential antimicrobial properties, inhibiting the growth of both Gram-negative and Gram-positive bacteria without antibiotic resistance.
Article
Nutrition & Dietetics
Gabriela N. Tenea, Pamela Ascanta
Summary: This study evaluated several cocoa semi-skimmed milk formulations as potential carriers of native lactic acid bacteria strains, finding that the addition of these strains improved bioactive molecules and maintained cell stability during storage. The study concluded that the final beverages can be regarded as functional food with improved characteristics due to LAB supplementation.
FRONTIERS IN NUTRITION
(2022)
Article
Infectious Diseases
Gabriela N. Tenea, Pamela Reyes, Diana Molina, Clara Ortega
Summary: This study analyzed the microbiological quality and safety of highly consumed fruits and juices in Imbabura Province, Ecuador, and found the presence of various pathogenic microorganisms and multidrug-resistant genes. Bacteriological analysis showed the detection of several bacterial indicators, including E. coli and Salmonella. Therefore, it is necessary to implement good agricultural and industrial practices, behavioral change communication, and awareness-raising programs to ensure food safety along the supply chain.
Article
Biochemistry & Molecular Biology
Gabriela N. Tenea
Summary: This study isolated a novel Lactococcus lactis strain from wild Amazonian camu-camu fruits, which contained various functional metabolites with antibacterial capacity. Genome analysis revealed high genetic diversity within the strain. Additionally, the strain was predicted to be non-pathogenic, lacked genes related to the production of biogenic amines and acquired antibiotic resistance genes. The study highlights the potential of this strain for producing bioactive metabolites for agri-food and pharmaceutical industry use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Gabriela N. Tenea, Blanca Anrango Cajas, Bladimir Carlosama Sanchez
Summary: This study evaluated the antibacterial activity of cell-free supernatant (CFS) from five native yeasts against indicator food pathogens. The generation of antimicrobials in the CFS was dependent on yeast strain and sugar-supplemented media. Treatment with proteinase K and catalase did not completely eliminate the inhibitory effect, suggesting that the active components are likely complex combinations of acids, proteins, hydrogen peroxide, and other metabolites.
Article
Agronomy
Gabriela Nicoleta Tenea
Summary: Food security is threatened by pathogens causing foodborne illnesses, especially in the consumption of fresh tropical fruits. Postharvest treatments are necessary for fruit preservation, and the use of bioactive compounds like postbiotics can offer a natural alternative for fruit protection.
AGROLIFE SCIENTIFIC JOURNAL
(2021)
Review
Biodiversity Conservation
Magdalena Diaz, Pablo Jarrin-V, Raquel Simarro, Pablo Castillejo, Gabriela N. Tenea, C. Alfonso Molina
Summary: The technological revolution has provided unprecedented access to molecular information, particularly evident in the microbiome field; The Ecuadorian Microbiome Project (EcuMP) aims to close the research gap in Ecuador's microbiome; There is a significant imbalance in scientific productivity related to microbiome and metagenomics in South American countries, highlighting the need for Ecuador to address technological dependency.
NEOTROPICAL BIODIVERSITY
(2021)
