Article
Immunology
Lin Xie, Wei Li, Wei-Ming Ye, Yao Xiao, Wu-Jian Ke, Jian-Jun Niu, Tian-Ci Yang
Summary: This study analyzed the levels of UCH-L1, GFAP, and NF-L in the serum of 153 patients without HIV and found that these proteins showed good diagnostic performance for neurosyphilis. The levels of these proteins in the serum correlated with those in CSF and had similar or better diagnostic performance. Therefore, these proteins could serve as biomarker candidates for neurosyphilis diagnosis without the need for lumbar puncture.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Minkyoung Sung, Soo-Eun Sung, Kyung-Ku Kang, Joo-Hee Choi, Sijoon Lee, KilSoo Kim, Ju-Hyeon Lim, Gun Woo Lee, Hyo-Deog Rim, Byung-Soo Kim, Seunghee Won, Kyungmin Kim, Seoyoung Jang, Min-Soo Seo, Jungmin Woo
Summary: This study analyzed miRNAs in serum-derived neuronal exosomes to investigate potential biomarkers of acute stress, revealing specific miRNAs in nEVs from an acute severe-stress animal model. These miRNAs show differential expression over time, with the most prominent brain damage observed 7 days after foot shock treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Minkyoung Sung, Soo-Eun Sung, Kyung-Ku Kang, Joo-Hee Choi, Sijoon Lee, KilSoo Kim, Ju-Hyeon Lim, Gun Woo Lee, Hyo-Deog Rim, Seunghee Won, Byung-Soo Kim, Kyungmin Kim, Seoyoung Jang, Sang Gyu Kwak, Jungmin Woo, Min-Soo Seo
Summary: Atopic dermatitis (AD) is a chronic allergic inflammatory skin disease characterized by symptoms like eczema and itching. Psychological stress can trigger neuroinflammation in the brain in AD patients, leading to changes in microRNAs (miRNAs) in neuronal exosomes (nEVs). This study found that certain miRNAs in nEVs may serve as stress-related biomarkers associated with AD and stress-induced depression.
Review
Oncology
Daniel B. Hier, Tayo Obafemi-Ajayi, Matthew S. Thimgan, Gayla R. Olbricht, Sima Azizi, Blaine Allen, Bassam A. Hadi, Donald C. Wunsch
Summary: Unresolved issues related to blood biomarkers after mild traumatic brain injury (mTBI) are mainly focused on the pathways of biomarkers entering and exiting the blood, the kinetics of blood biomarkers, and the predictive capacity of blood biomarkers after mTBI. Further research is needed to address these issues.
BIOMARKER RESEARCH
(2021)
Article
Infectious Diseases
Qiu-Yue Ning, Na Liu, Ji-Zhou Wu, Die-Fei Hu, Qi Wei, Jin-Ai Zhou, Jun Zou, Ning Zang, Guo-Jian Li
Summary: This study investigated serum exosomal miRNAs in AIDS patients with TM infection, identifying three potential biomarkers and exploring their biological functions and pathways.
INFECTION AND DRUG RESISTANCE
(2021)
Review
Biochemistry & Molecular Biology
Giada Giovannini, Stefano Meletti
Summary: According to the latest ILAE definition, status epilepticus can lead to long-term irreversible consequences, including neuronal death, neuronal injury, and alterations in neuronal networks. Therefore, there is growing interest in identifying biomarkers that can demonstrate and quantify the extent of neuro-glial injury. Although numerous studies have been conducted on animal models, evidence in humans is limited to case reports and small case series. The identification of these biomarkers in clinical practice can have significant implications, such as improved prognostic stratification and early identification of at-risk patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Chenbin Liu, Han Wu, Yinqi Mao, Wei Chen, Shuying Chen
Summary: Exosomal microRNAs play a crucial role in hepatocellular carcinoma, holding significant value in the early diagnosis and treatment of this disease.
CANCER CELL INTERNATIONAL
(2021)
Article
Critical Care Medicine
Sophie Richter, Stefan Winzeck, Endre Czeiter, Krisztina Amrein, Evgenios N. Kornaropoulos, Jan Verheyden, Gabriela Sugar, Zhihui Yang, Kevin Wang, Andrew I. R. Maas, Ewout Steyerberg, Andras Buki, Virginia F. J. Newcombe, David K. Menon
Summary: Serum protein biomarkers show potential to safely reduce the number of transfers to MRI in critically ill patients with moderate-severe TBI.
Article
Clinical Neurology
Ana M. Castano-Leon, Cristina Sanchez Carabias, Amaya Hilario, Ana Ramos, Blanca Navarro-Main, Igor Paredes, Pablo M. Munarriz, Irene Panero, Carla Eiriz Fernandez, Daniel Garcia-Perez, Luis Miguel Moreno-Gomez, Olga Esteban-Sinovas, Guillermo Garcia Posadas, Pedro A. Gomez, Alfonso Lagares
Summary: This study explores the potential role of serum biomarkers in assessing traumatic axonal injury (TAI). By extracting blood and performing imaging assessments on traumatic brain injury (TBI) patients, it was found that the concentrations of biomarkers were significantly higher in TBI patients compared to the control group. Among these biomarkers, UCH-L1 and NfL appear to be more specific markers for detecting TAI. Furthermore, the combination of NfL and the fractional anisotropy (FA) of the corpus callosum (CC) can help predict long-term outcomes.
JOURNAL OF NEUROSURGERY
(2023)
Article
Medical Laboratory Technology
Charlotte Oris, Jean-Baptiste Bouillon-Minois, Samy Kahouadji, Bruno Pereira, Gabriel Dhaiby, Valentin Bailly Defrance, Julie Durif, Jeannot Schmidt, Fares Moustafa, Damien Bouvier, Vincent Sapin
Summary: Measuring plasma GFAP and UCH-L1 levels is useful in targeting and optimizing the reduction of CT scans in mTBI patients.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Critical Care Medicine
Linda Papa, Alexa E. Walter, James R. Wilkes, Hunter S. Clonts, Brian Johnson, Semyon M. Slobounov
Summary: This study examined the changes in brain injury biomarker concentrations in different types of football athletes and found that players in speed and Profile 1 positions had the highest concentrations of GFAP, Tau, and NF-L, while players in non-speed and Profile 3 positions had the lowest concentrations.
JOURNAL OF NEUROTRAUMA
(2022)
Article
Endocrinology & Metabolism
Federica Ferrari, Daniela Rossi, Alessandra Ricciardi, Carlo Morasso, Liliana Brambilla, Sara Albasini, Renzo Vanna, Chiara Fassio, Tatjana Begenisic, Marianna Loi, Daniela Bossi, Alberto Zaliani, Elisa Alberici, Claudio Lisi, Andrea Morotti, Anna Cavallini, Federico Mazzacane, Antonio Nardone, Fabio Corsi, Marta Truffi
Summary: This study aimed to measure the serum levels of neurofilament light chain (NfL) and glial fibrillary acidic protein (GFAP) in stroke patients and evaluate their correlation with functional outcome and rehabilitation scores. The results showed that serum NfL and GFAP levels were positively correlated with clinical/rehabilitation outcomes, with NfL being the best predictor.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2023)
Review
Medicine, General & Internal
Angel Earle, Madison Bessonny, Josh Benito, Kun Huang, Hannah Parker, Emily Tyler, Brittany Crawford, Nabeeha Khan, Bridget Armstrong, Alexis Stamatikos, Sudha Garimella, Alyssa Clay-Gilmour
Summary: The early detection of chronic kidney disease is crucial, and there is growing interest in finding new biomarkers. Currently, there is no single effective biomarker, but there is evidence supporting the use of urinary exosomal microRNAs as biomarkers for kidney fibrosis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Se Jun Park, Ju Yeon Park, Kabsoo Shin, Tae Ho Hong, MyungAh Lee, Younghoon Kim, In-Ho Kim
Summary: This study investigated the correlation between circulating exosomal PD-L1 (exoPD-L1) and PD-L1 expression in tumor tissue, as well as the impact on survival outcomes in patients with advanced PDAC. The results showed that the levels of exoPD-L1 were higher in patients with metastatic pancreatic cancer compared to those with locally advanced disease, and were also higher in patients with positive PD-L1 expression. Higher levels of exoPD-L1 were associated with worse survival outcomes.
Review
Critical Care Medicine
Alice Rogan, Morgane Brunton O'Sullivan, Ana Holley, David McQuade, Peter Larsen
Summary: This study aimed to assess the use of serum and plasma biomarkers to exclude significant intracranial injuries in TBI patients and found that S100B is currently the only biomarker with moderate quality evidence to support its use.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2022)
Review
Spectroscopy
Wenjing Peng, Cristian D. Gutierrez Reyes, Sakshi Gautam, Aiying Yu, Byeong Gwan Cho, Mona Goli, Kaitlyn Donohoo, Stefania Mondello, Firas Kobeissy, Yehia Mechref
Summary: Glycosylation is a significant posttranslational modification in mammalian cells, involved in various biofunctions and diseases. Mass spectrometry is a powerful tool for glycan and glycopeptide isomeric analysis, but its comprehensive characterization remains a challenge. Separation methods and bioinformatic tools are applied to address this challenge.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Public, Environmental & Occupational Health
Periklis Charalampous, Juanita A. Haagsma, Lea S. Jakobsen, Vanessa Gorasso, Isabel Noguer, Alicia Padron-Monedero, Rodrigo Sarmiento, Joao Vasco Santos, Scott A. McDonald, Dietrich Plass, Grant M. A. Wyper, Ricardo Assuncao, Elena von der Lippe, Balazs Adam, Ala'a AlKerwi, Jalal Arabloo, Ana Lucia Baltazar, Boris Bikbov, Maria Borrell-Pages, Iris Brus, Genc Burazeri, Serafeim C. Chaintoutis, Jose Chen-Xu, Nino Chkhaberidze, Seila Cilovic-Lagarija, Barbara Corso, Sarah Cuschieri, Carlotta Di Bari, Keren Dopelt, Mary Economou, Theophilus Emeto, Peter Fantke, Florian Fischer, Alberto Freitas, Juan Manuel Garcia-Gonzalez, Federica Gazzelloni, Mika Gissler, Artemis Gkitakou, Hakan Gulmez, Sezgin Gunes, Sebastian Haller, Romana Haneef, Cesar A. Hincapie, Paul Hynds, Jane Idavain, Milena Ilic, Irena Ilic, Gaetano Isola, Zubair Kabir, Maria Kamusheva, Pavel Kolkhir, Naime Meric Konar, Polychronis Kostoulas, Mukhtar Kulimbet, Carlo La Vecchia, Paolo Lauriola, Miriam Levi, Marjeta Majer, Enkeleint A. Mechili, Lorenzo Monasta, Stefania Mondello, Javier Munoz Laguna, Evangelia Nena, Edmond S. W. Ng, Paul Nguewa, Vikram Niranjan, Iskra Alexandra Nola, Ronan O'Caoimh, Marija Obradovic, Elena Pallari, Mariana Peyroteo, Vera Pinheiro, Nurka Pranjic, Miguel Reina Ortiz, Silvia Riva, Cornelia Melinda Adi Santoso, Milena Santric Milicevic, Tugce Schmitt, Niko Speybroeck, Maximilian Sprugel, Paschalis Steiropoulos, Aleksandar Stevanovic, Lau Caspar Thygesen, Fimka Tozija, Brigid Unim, Hilal Bektas Uysal, Orsolya Varga, Milena Vasic, Rafael Jose Vieira, Vahit Yigit, Brecht Devleesschauwer, Sara M. Pires
Summary: This systematic literature review provides an overview of studies applying the disability-adjusted life years (DALY) concept for infectious diseases in European countries. Among the 105 included studies, food- and water-borne diseases were the most frequently studied. The number of burden of infectious disease studies has increased over time, but there is less consensus in methodological approaches.
EPIDEMIOLOGY AND INFECTION
(2023)
Article
Medicine, Research & Experimental
Stanley Ibeh, Nour-Mounira Z. Bakkar, Fatima Ahmad, Judith Nwaiwu, Chloe Barsa, Sarine Mekhjian, Mohammad Amine Reslan, Ali H. Eid, Hayat Harati, Sanaa Nabha, Yehia Mechref, Ahmed F. El-Yazbi, Firas Kobeissy
Summary: This study suggests that chronic high-fat diet worsens the outcomes of traumatic brain injury, leading to neurodegeneration and impaired brain recovery.
Article
Neurosciences
Hamad Yadikar, Connor Johnson, Niko Pafundi, Lynn Nguyen, Milin Kurup, Isabel Torres, Albandery Al-Enezy, Zhihui Yang, Richard Yost, Firas H. Kobeissy, Kevin K. W. Wang
Summary: Traumatic brain injury (TBI) is a complex damage with no approved medicine. Using a mouse model of TBI, this study investigated the tau proteolytic peptidome and phosphorylation sites associated with the injury. The findings revealed hyperphosphorylation and breakdown products of tau in response to TBI, along with unique sequences of proteolytic peptides derived from human tau protein.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Behavioral Sciences
Dania Kattan, Chloe Barsa, Sarin Mekhijian, Zaynab Shakkour, Maya Jammoul, Mark Doumit, Maria Camila Pareja Zabala, Nadine Darwiche, Ali H. Eid, Yehia Mechref, Kevin K. Wang, Juan Pablo de Rivero Vaccari, Jennifer C. Munoz Pareja, Firas Kobeissy
Summary: Given the lack of FDA-approved neurotherapeutic drugs and the ambiguity surrounding TBI pathophysiology, it is crucial to understand the mechanisms of TBI and explore potential therapeutic targets. Inflammasomes have emerged as important players in the CNS response to neurological conditions, including TBI and other neurodegenerative disorders. This review provides an overview of the activation and assembly mechanisms of inflammasomes and discusses their implications in TBI and other neurodegenerative diseases. It also explores the potential of utilizing inflammasomes as biomarkers and therapeutic targets for TBI.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2023)
Editorial Material
Clinical Neurology
Anastasia Bougea, Per Svenningsson, Ioanna Markaki, Abdul Hye, Stefania Mondello
FRONTIERS IN NEUROLOGY
(2023)
Review
Neurosciences
Maya Jammoul, Dareen Jammoul, Kevin K. Wang, Firas Kobeissy, Ralph G. Depalma
Summary: This article reviews the possible mechanisms by which traumatic brain injury (TBI) may stimulate the development of opioid use disorder (OUD) and discusses the interaction between these two processes. CNS damage due to TBI appears to drive adverse effects of subsequent OUD, with pain being a risk factor for opioid use after TBI.
BIOLOGICAL PSYCHIATRY
(2024)
Article
Cell Biology
Alice Capuz, Sylvain Osien, Melodie Anne Karnoub, Soulaimane Aboulouard, Estelle Laurent, Etienne Coyaud, Antonella Raffo-Romero, Marie Duhamel, Amelie Bonnefond, Mehdi Derhourhi, Marco Trerotola, Ikram El Yazidi-Belkoura, David Devos, Monika Zilkova, Firas Kobeissy, Fabien Vanden Abeele, Isabelle Fournier, Dasa Cizkova, Franck Rodet, Michel Salzet
Summary: Using various omics analyses, we discovered that newborn rat astrocytes produce neural immunoglobulin chains with similarities to aberrant immunoglobulins found in cancers. The complete enzymatic V(D)J recombination complex was also found in astrocytes. In addition, we identified different immunoglobulin chains in adult rat astrocytes and primary astrocytes from human fetus. These neural IgGs play a role in astrocyte fate and interact with specific targets.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Akeem Sanni, Mona Goli, Jingfu Zhao, Junyao Wang, Chloe Barsa, Samer El Hayek, Farid Talih, Bartolo Lanuzza, Firas Kobeissy, Giuseppe Plazzi, Monica Moresco, Stefania Mondello, Raffaele Ferri, Yehia Mechref
Summary: This study aimed to identify potential serum biomarkers for narcolepsy type 1 (NT1), a life-threatening disorder characterized by a sleep-wake cycle disorder. A proteomics analysis was conducted, which revealed 36 differentially expressed proteins associated with NT1. These findings provide insights into the pathophysiological effects and important biochemical pathways involved in NT1.
Article
Chemistry, Analytical
Peilin Jiang, Wenjing Peng, Jingfu Zhao, Mona Goli, Yifan Huang, Yunxiang Li, Yehia Mechref
Summary: The complexity and heterogeneity of protein glycosylation present challenges in characterization and quantitation. This study introduces a metabolic isotope labeling strategy called GlyProSILC, which can label both the glycan motif and peptide backbone in the same batch of cells. It provides an accurate and effective quantitation approach for glycomics, proteomics, and glycoproteomics in a cell culture system.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Chirine El-Baba, Zeinab Ayache, Mona Goli, Berthe Hayar, Zeinab Kawtharani, Claudio Pisano, Firas Kobeissy, Yehia Mechref, Nadine Darwiche
Summary: The study shows that ST1926 is effective in reducing cell viability and inducing apoptosis in GBM cells, and it also reduces the level of POLA1 protein. Additionally, proteomics analysis reveals that ST1926 affects multiple cellular processes related to GBM cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Junyao Wang, Aiying Yu, Byeong Gwan Cho, Yehia Mechref
Summary: Retention time is a crucial parameter in liquid chromatography (LC) for demonstrating separation results. However, it can vary when samples are tested with different instruments and labs, making it difficult to compare data from different LC systems. To address this issue, a hydrophobicity index was introduced to normalize the retention time of glycopeptides separated by reversed-phase LC (RPLC). Tandem MS was used for glycopeptide detection and identification.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Editorial Material
Clinical Neurology
Wael M. Y. Mohamed, Firas Kobeissy, Stefania Mondello
FRONTIERS IN NEUROLOGY
(2023)
Meeting Abstract
Critical Care Medicine
Jennifer Munoz Pareja, Maria Mateo Chavez, Juan Pablo De Rivero Vaccari, Maria Kerrigan, Charlene Pringle, Firas H. Kobeissy, Kourtney Guthrie, Joslyn Gober, Rajderkar Dhanashree, Nora AlFakhri, Jennifer Coto, Paula Karina Perez, Heather J. McCrea, Chad M. Thorson, Kristine H. O'Phelan, Juan P. Solano, Kathryn Swaby, Ricardo Loor Torres, Kevin K. Wang
CRITICAL CARE MEDICINE
(2023)
Review
Oncology
Reem Halabi, Fatima Dakroub, Mohammad Z. Haider, Stuti Patel, Nayef A. Amhaz, Mohammad A. Reslan, Ali H. Eid, Yehia Mechref, Nadine Darwiche, Firas Kobeissy, Ibrahim Omeis, Abdullah A. Shaito
Summary: This review discusses the genetic and epigenetic abnormalities in meningiomas, with a focus on neurofibromatosis type 2 and non-NF2 mutations. It also explores novel biomarkers for meningioma diagnosis and prognosis, including epigenetic-, RNA-, metabolomics-, and protein-based markers. Additionally, the available meningioma-specific animal models are overviewed.