Article
Pharmacology & Pharmacy
Silvia Pisani, Valeria Mauri, Erika Negrello, Valeria Friuli, Ida Genta, Rossella Dorati, Giovanna Bruni, Stefania Marconi, Ferdinando Auricchio, Andrea Pietrabissa, Marco Benazzo, Bice Conti
Summary: In this study, hybrid scaffolds were developed as a versatile platform for soft tissue regeneration. These scaffolds exhibited favorable cell adhesion and proliferation, and allowed for controlled drug release.
Review
Polymer Science
Nurulhuda Arifin, Izman Sudin, Nor Hasrul Akhmal Ngadiman, Mohamad Shaiful Ashrul Ishak
Summary: This article reviews the potential of photo-polymerization 3D printing technique in tissue engineering scaffold fabrication. It also compares this technique with other scaffold fabrication methods. Various parameter settings affecting mechanical properties, biocompatibility, and porosity behavior are discussed in detail.
Article
Engineering, Biomedical
Dewei Qiu, Chuanliang Cao, Aruna Prasopthum, Zhenchang Sun, Shan Zhang, Hanwen Yang, Zhiyong Xu, Jun Tao, Fanrong Ai, Jing Yang
Summary: The study investigated the interfacial bonding formation and the role of immune cells in bone-scaffold material interfaces. Different levels of foreign body response (FBR) were elicited by two different 3D printed porous scaffolds, with polymer/hydroxyapatite composite scaffolds showing more intensive FBR compared to sintered hydroxyapatite scaffolds. The presence of FBR components appeared to inhibit osseointegration.
MATERIALS TODAY BIO
(2023)
Article
Nanoscience & Nanotechnology
Kateryna Diedkova, Alexander D. Pogrebnjak, Sergiy Kyrylenko, Kateryna Smyrnova, Vladimir V. Buranich, Pawel Horodek, Pawel Zukowski, Tomasz N. Koltunowicz, Piotr Galaszkiewicz, Kristina Makashina, Vitaly Bondariev, Martin Sahul, Maria Caplovicova, Yevheniia Husak, Wojciech Simka, Viktoriia Korniienko, Agnieszka Stolarczyk, Agata Blacha-Grzechnik, Vitalii Balitskyi, Veronika Zahorodna, Ivan Baginskiy, Una Riekstina, Oleksiy Gogotsi, Yury Gogotsi, Maksym Pogorielov
Summary: New conductive materials are needed for tissue engineering to develop regenerative strategies for nervous, muscular, and heart tissues. Polycaprolactone (PCL) is used to create biocompatible and biodegradable nanofiber scaffolds, while MXenes can make these scaffolds conductive and hydrophilic. This study investigates the physical properties, defect structure, and porosity of PCL-MXene composite scaffolds, and demonstrates their advantages over existing conductive scaffolds for tissue engineering in terms of structure, chemistry, electricity, and biology.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biotechnology & Applied Microbiology
Sebastian Loewner, Sebastian Heene, Timo Baroth, Henrik Heymann, Fabian Cholewa, Holger Blume, Cornelia Blume
Summary: Melt electro writing (MEW) is a high-resolution 3D printing technique that deposits micro- to nanometer strands of biocompatible polymers layer by layer, making it a promising method for tissue engineering scaffold fabrication. This review discusses optimization strategies for precise fiber deposition, printing protocols for different scaffold structures, and the combination of MEW with other fabrication techniques to create advanced scaffold structures.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Thiago Domingues Stocco, Mayara Cristina Moreira Silva, Marcus Alexandre Finzi Corat, Gabriely Goncalves Lima, Anderson Oliveira Lobo
Summary: This study presents a new biofabrication strategy for developing biomimetic meniscus scaffolds. The use of bioprinted cell-laden hydrogel reinforced by aligned nanofiber sheets improved the structural integrity, shape fidelity, and mechanical properties of the scaffolds. Importantly, the introduction of nanofiber mats did not negatively affect cell viability. This bioengineering approach offers a promising strategy for tissue engineering of the knee meniscus.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Otorhinolaryngology
Hanaro Park, In Gul Kim, Yanru Wu, Hana Cho, Jung-Woog Shin, Su A. Park, Eun-Jae Chung
Summary: After evaluating esophageal reconstructions using different scaffolds, it was found that 3DP PCL scaffolds had better muscle regeneration while PU-Nf scaffolds had better epithelial regeneration.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2021)
Review
Chemistry, Physical
Angelika Zaszczynska, Maryla Moczulska-Heljak, Arkadiusz Gradys, Pawel Sajkiewicz
Summary: This study summarizes the latest advancements in 3D printing techniques in tissue engineering applications, focusing on the systematics of available 3D printing techniques, printable biomaterials, and advancements in 3D printing-assisted tissue engineering.
Review
Chemistry, Multidisciplinary
Assaf Shapira, Tal Dvir
Summary: 3D bioprinting is an emerging technology with great potential in tissue engineering. Despite challenges, innovative approaches and advanced technologies can address current issues and pave the way for future directions.
Review
Pharmacology & Pharmacy
Amber F. MacDonald, Meaghan E. Harley-Troxell, Steven D. Newby, Madhu S. Dhar
Summary: This article summarizes the application of graphene-based materials in tissue engineering, with a focus on bone tissue engineering. It discusses the need for developing 3D graphene scaffolds and introduces the current manufacturing techniques and limitations.
Review
Polymer Science
Naiyu Cui, Chun-Yu Dai, Xuran Mao, Xun Lv, Yue Gu, Eui-Seok Lee, Heng-Bo Jiang, Yunhan Sun
Summary: Poloxamer, as a triblock copolymer with amphiphilicity and reversible thermal responsiveness, has wide application prospects in biomedicine. It plays a crucial role as injectable scaffolds in tissue engineering and 3D printing, creating an appropriate microenvironment for cell growth and vascular network formation.
Article
Chemistry, Physical
Ghazal Belgheisi, Masoumeh Haghbin Nazarpak, Mehran Solati-Hashjin
Summary: This study presents a new approach to produce tissue engineering scaffolds by combining electrospinning and additive manufacturing technologies. The fabricated 3D-structured scaffolds consist of pamidronate-loaded layered double hydroxides/polycaprolactone electrospun nanofibers and micro-strand polycaprolactone 3D printed grids. The scaffolds show reduced fiber diameter and increased surface roughness when pamidronate-loaded layered double hydroxides are added to polycaprolactone. The final structure of the scaffolds is obtained by gluing the nanofiber mats between 3D printed micro-strand grids. The scaffolds demonstrate suitable pore size, controllable drug release, and enhanced cell adhesion and osteoconductivity.
APPLIED CLAY SCIENCE
(2022)
Article
Chemistry, Applied
Fatemeh Asghari, Davood Rabiei Faradonbeh, Ziba Veisi Malekshahi, Houra Nekounam, Behnaz Ghaemi, Yaser Yousefpoor, Hossein Ghanbari, Reza Faridi-Majidi
Summary: In this study, a new hybrid nanofibrous scaffold composed of polycaprolactone/chitosan-polyethylene oxide (PCL/Cs-PEO) incorporated with Arnebia euchroma (A. euchroma) extract was synthesized by the two-nozzle electrospinning method. The scaffold showed improved and increased proliferation of skin cells in vitro, confirming its potential for skin tissue engineering.
CARBOHYDRATE POLYMERS
(2022)
Review
Biotechnology & Applied Microbiology
Maria Grazia Tupone, Michele d'Angelo, Vanessa Castelli, Mariano Catanesi, Elisabetta Benedetti, Annamaria Cimini
Summary: Exploring and developing multifunctional intelligent biomaterials is crucial for improving next-generation therapies in tissue engineering and regenerative medicine. These biomaterials can mimic the in situ microenvironment and provide specialized bioactive signals to regulate the surrounding biological habitat, contributing to wound healing and biomaterial integration. The connection between stem cells and biomaterials can stimulate specific modifications in cell properties and fate, influencing processes such as self-renewal, adhesion, and differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Polymer Science
Evangelos Daskalakis, Boyang Huang, Cian Vyas, Anil Ahmet Acar, Ali Fallah, Glen Cooper, Andrew Weightman, Bahattin Koc, Gordon Blunn, Paulo Bartolo
Summary: This study proposes a method for preparing polycaprolactone/bioglass scaffolds with pore size gradient by investigating the coupled effect between material composition and scaffold architecture. The results show that the addition of bioglass improves the mechanical properties and biological performance of the scaffolds.
Article
Chemistry, Physical
Enes Aslan, Cian Vyas, Joel Yupanqui Mieles, Gavin Humphreys, Carl Diver, Paulo Bartolo
Summary: Skin is a crucial organ exposed to external environment, with wounds potentially leading to death. Electrospinning technology can accelerate skin healing, while honey shows promise as a biomaterial. Nanofibrous meshes incorporating honey exhibit good biocompatibility and potential for skin applications.
Article
Polymer Science
Evangelos Daskalakis, Boyang Huang, Cian Vyas, Anil Ahmet Acar, Ali Fallah, Glen Cooper, Andrew Weightman, Bahattin Koc, Gordon Blunn, Paulo Bartolo
Summary: This study proposes a method for preparing polycaprolactone/bioglass scaffolds with pore size gradient by investigating the coupled effect between material composition and scaffold architecture. The results show that the addition of bioglass improves the mechanical properties and biological performance of the scaffolds.
Article
Polymer Science
Julia Venturini Helaehil, Carina Basqueira Lourenco, Boyang Huang, Luiza Venturini Helaehil, Isaque Xavier de Camargo, Gabriela Bortolanca Chiarotto, Milton Santamaria-Jr, Paulo Bartolo, Guilherme Ferreira Caetano
Summary: Composite scaffolds made of polycaprolactone (PCL) mixed with bioceramics, such as hydroxyapatite (HA) and tricalcium phosphate (TCP), showed improved biological recognition and bioactivity. Electrical stimulation (ES) enhanced cell proliferation and differentiation. The study revealed that the combined use of PCL with HA or TCP scaffolds and ES improved bone regeneration.
Article
Engineering, Chemical
Gokhan Hekimoglu, Ahmet Sari, Yunus Onal, Osman Gencel, V. V. Tyagi, Enes Aslan
Summary: In this study, low-cost and eco-friendly activated carbon obtained from waste apricot kernel shells was used to improve the thermal conductivity of organic phase change materials (Capric-Myristic acid, Lauryl alcohol, n-Octadecane, and Polyethylene glycol) while addressing their inherent drawbacks. The composites showed high loading rates and latent heat capacity of the phase change materials, as well as excellent thermal degradation resistance and cycling stability. The thermal conductivity of the composites was significantly higher than that of the pure phase change materials, making them suitable for low-temperature thermal management of buildings.
Article
Engineering, Industrial
Paulo Bartolo, Ajay Malshe, Eleonora Ferraris, Bahattin Koc
Summary: Ageing population and new diseases require the development of novel therapeutic strategies. 3D bioprinting, as a potential transformative strategy in tissue engineering and regenerative medicine, is introduced. The concept of 3D bioprinting is discussed, and the key requirements of bio-inks and main materials used for cell encapsulation are detailed. Recent advances in the use of smart materials and the concept of 4D printing are also explored. The main techniques of 3D bioprinting are described in detail and key limitations are highlighted. Successful cases that demonstrate the relevance of 3D bioprinting are presented. Finally, the paper addresses the main research challenges and future perspectives in the field of 3D bioprinting.
CIRP ANNALS-MANUFACTURING TECHNOLOGY
(2022)
Article
Engineering, Mechanical
Senol Sirin, Enes Aslan, Gulsah Akincioglu
Summary: The purpose of this study was to investigate the friction performance of 3D-printed polylactic acid (PLA) at different infill densities. PLA samples were printed using fused filament fabrication (FFF), and the friction performance was tested using a pin-on-disc tester. The results showed that the sample with 50% infill density had the least deformation during the 20-minute test period. This study contributes to the literature by providing friction coefficient results for different infill densities of 3D-printed PLA.
RAPID PROTOTYPING JOURNAL
(2023)
Review
Oncology
Samantha Fernandes, Cian Vyas, Peggy Lim, Ruben F. Pereira, Amaya Viros, Paulo Bartolo
Summary: Melanoma, a potentially fatal cancer, has been increasing in incidence over the past 50 years due to excessive sun exposure and ultraviolet radiation. With the highest incidence in people of European descent and the aging population, melanoma is influenced by various clinical and epidemiological factors such as sex, ethnicity, UV exposure, anatomic site, and age. Although improvements in targeted and immunotherapies have led to better survival rates, advancing our understanding of melanoma biology and disease progression is crucial for improving clinical outcomes. The development of three-dimensional human skin equivalents using biofabrication techniques, like bioprinting, offers a promising approach to unravel the complexity of melanoma and associated risk factors. These 3D skin models can serve as a platform for personalized and therapeutic testing.
Review
Pharmacology & Pharmacy
Joel Yupanqui Mieles, Cian Vyas, Enes Aslan, Gavin Humphreys, Carl Diver, Paulo Bartolo
Summary: Honey is experiencing a resurgence in evaluation for antimicrobial and wound healing applications due to its ability to inhibit various bacteria, fungi, and viruses, as well as promote wound healing. Its activity is attributed to the enzymatic generation of hydrogen peroxide and other non-peroxide factors. The development of tissue engineering approaches has been driven by the difficulty of delivering honey's active ingredients to the wound site.
Article
Chemistry, Multidisciplinary
Boyang Huang, Yaxin Wang, Cian Vyas, Paulo Bartolo
Summary: This study compares two different extrusion additive manufacturing techniques for fabricating polymer-based 3D bone scaffolds. The results show that the scaffolds produced using these techniques exhibit distinct physiochemical properties. Melt-printed scaffolds have stronger mechanical properties, while solvent-printed scaffolds have a rougher surface, higher degradation rate, and faster stress relaxation. These differences are attributed to the different crystal growth kinetics and result in different cell-scaffold interactions.
Review
Engineering, Biomedical
Fengyuan Liu, Rixiang Quan, Cian Vyas, Enes Aslan
Summary: Scaffold-based approach is a developed strategy in biomanufacturing that relies on temporary scaffold for cell attachment, proliferation, and differentiation. However, single material scaffolds often fail to meet all requirements, leading to the investigation of multi-material and mixing-material scaffolds. Hybrid bioprinting techniques have been developed to overcome challenges in resolution, cell distribution, and tissue formation. This review introduces different bioprinting systems and their effects on complex tissue regeneration applications.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Mechanical
Guelsah Akincioglu, Emine Sirin, Enes Aslan
Summary: This study investigates the effect of different infill densities on the wear properties of ABS polymer samples. The results show that infill density is an important factor affecting the friction and heat characteristics of ABS samples, with higher infill densities resulting in higher friction and heat.
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART J-JOURNAL OF ENGINEERING TRIBOLOGY
(2023)
Review
Engineering, Manufacturing
Yaxin Wang, Ruben F. Pereira, Chris Peach, Boyang Huang, Cian Vyas, Paulo Bartolo
Summary: Articular cartilage damage can cause pain, mobility issues, and disability. Current surgical treatments for cartilage repair are limited. Three-dimensional bioprinting has been used to mimic the native microenvironment and promote tissue regeneration, but conventional in vitro bioprinting has limitations. In situ bioprinting, a novel strategy that directly delivers bioinks to the desired site, shows potential to overcome these limitations. This review focuses on robotic-assisted in situ bioprinting surgical systems for cartilage regeneration, discussing existing approaches and potential future perspectives.
INTERNATIONAL JOURNAL OF EXTREME MANUFACTURING
(2023)
Article
Polymer Science
Paulo Roberto Lopes Nalesso, Matheus Vedovatto, Julia Eduarda Schneider Gregorio, Boyang Huang, Cian Vyas, Milton Santamaria-Jr, Paulo Bartolo, Guilherme Ferreira Caetano
Summary: This study evaluated the use of 3D printed PCL-based composite scaffolds containing CNTs, HA, and β-TCP in a critical bone defect rat model. The results showed that the CNT+HA/TCP group had higher expression of osteogenic genes after 7 days, while the CNT+HA and CNT+TCP groups stimulated higher gene expression for tissue formation and mineralization, as well as pro- and anti-inflammatory genes after 14 and 30 days. The association of CNTs with ceramics promoted a minor inflammatory response and faster bone tissue formation.
Review
Engineering, Electrical & Electronic
Idil Tartici, Zekai Murat Kilic, Paulo Bartolo
Summary: Three-dimensional printing, or additive manufacturing, has the potential to revolutionize traditional manufacturing systems by producing high-value-added complex products at reduced costs, making it a key technology in the Industry 4.0 era. This systematic literature review analyzes ongoing research on 3D technologies in the field of monitoring and control to facilitate their adoption in Industry 4.0.