Article
Food Science & Technology
Iththichai Chimpanao, Suwattana Pruksasri
Summary: The beta-mannanase present in the commercial enzyme Pectinex Ultra SP-L exhibited efficient activity in hydrolyzing copra meal to produce mannooligosaccharides (MOS). Optimum mannanase activity was observed at pH 4.0 and a temperature of 65 degrees C. The hydrolysate obtained from the hydrolysis of copra meal using Pectinex Ultra SP-L contained approximately 45% oligosaccharides, with mannobiose being the predominant component. This study presents a simple and efficient method for obtaining oligosaccharides from copra meal using a commercial pectinolytic enzyme.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jurairat Rungruangsaphakun, Massalin Nakphaichit, Suttipun Keawsompong
Summary: This study investigated the optimal hydrolysis conditions for improving copra meal using mannanase from Bacillus circulans in a fed-batch setting. The effects of hydrolyzed copra meal (HCM) and conventional copra meal (CM) on simulated swine gastrointestinal digestion were also studied. The experimental results showed that fed-batch hydrolysis under optimal conditions resulted in higher reducing sugar and total oligosaccharide yield compared to batch hydrolysis. Mannanase also reduced the fiber content and caused significant degradation of copra meal microfibrils. The simulated digestion revealed that HCM was more easily digested than CM.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Vitor S. Haetinger, Chan Sol Park, Olayiwola Adeola
Summary: Two studies were conducted with broiler chickens to determine the ileal digestible energy (IDE), ME, and MEn in copra meal (CM) and cornstarch. The results showed that inclusion of CM in diets may reduce the digestibility of GE, whereas the digestibility and utilization of GE may increase when adding cornstarch into diets for broiler chickens.
Article
Agriculture, Dairy & Animal Science
Jae Cheol Jang, Dong Hyuk Kim, Young Dal Jang, Yoo Yong Kim
Summary: The study aimed to estimate the standardized ileal digestibility (SID) of amino acids in growing pigs fed diets with increasing levels of copra meal. The results showed that there were linear decreases in the digestibility of certain amino acids with increasing levels of copra meal, while the effect of 3-mannanase supplementation on amino acid digestibility was not significant.
Article
Food Science & Technology
Qian Zhang, Zhipeng Yu, Wenzhu Zhao
Summary: This study aimed to identify novel antioxidant peptides from copra meal protein and confirmed the scavenging activity of peptides GMEEER and EEGER on free radicals. Molecular docking and isothermal titration calorimetry were used to explore the mechanism of antioxidant peptides in scavenging free radicals, and the results showed that hydrophobic interactions and hydrogen bonding were the major driving forces. These findings revealed the antioxidant mechanism of copra meal protein and provided theoretical support for its high-value utilization.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Witida Sathitkowitchai, Ponsit Sathapondecha, Pacharaporn Angthong, Yanee Srimarut, Yuwares Malila, Wuttichai Nakkongkam, Sage Chaiyapechara, Nitsara Karoonuthaisiri, Suttipun Keawsompong, Wanilada Rungrassamee
Summary: This study isolated two bacterial strains, Man26 and Man122, from shrimp intestines and found that they have the ability to produce mannanase, an enzyme that digests MOS and other mannan-rich materials. These strains can tolerate a wide pH range and their crude enzymes significantly increased the release of sugars and proteins from copra meal, palm kernel cake, and soybean meal. These findings suggest that these bacterial strains could potentially be used in animal feeds to improve the utilization of feedstuffs.
Article
Biochemistry & Molecular Biology
A. Shubhashini, Neelam Prabha, P. Monica, Sachin Rama Chaudhari, Mukesh Kapoor
Summary: Size-exclusion chromatography, HR-ESI-MS, and FT-IR analysis revealed the presence of oligosaccharides (CM-beta-MOS) with a degree of polymerisation (DP) between 2 and 4 in hydrolyzed copra meal by ManB-1601. Thermal decomposition studies showed mass loss at high temperatures for purified CM-beta-MOS (DP 2, 3, and 4). NMR studies confirmed the chemical structure of CM-beta-MOS. During fermentation, purified CM-beta-MOS supported the growth of Lactobacillus sp. and inhibited enteropathogens. RT-PCR studies suggested up-regulation of specific genes in Lactobacillus sp. fed with CM-beta-MOS. Biochemical, fluorescence, and molecular docking studies indicated the potential anti-glycation effects of CM-beta-MOS.
Article
Agriculture, Dairy & Animal Science
S. Retnaningrum, Kusmartono, Mashudi, K. J. Harper, D. P. Poppi
Summary: Formulating rations with high energy and protein feeds like cassava and locally sourced protein meals is crucial for increasing live weight gain of crossbred bulls in Indonesia. An experiment showed that a combination of 40% dried cassava meal and 40% protein meals maximized intake and live weight gain, with a marked decrease in intake and weight gain beyond that level of cassava meal inclusion.
Review
Food Science & Technology
Tzodoq Obrero Magbanua, Janice Alano Ragaza
Summary: This paper comprehensively evaluated the growth performance and carcass composition of Nile tilapia fed copra meal through a systematic review and meta-analysis. The results showed that the control group had better growth and feed utilization parameters compared to the plant-based diets. However, there was no significant difference in the carcass composition between fish fed copra meal-based diet and fish fed fishmeal-based diet, except for ash content. Further meta-regression analysis revealed that factors such as feeding period, crude protein level, fish development stage, and dietary copra inclusions did not affect the heterogeneity of the data.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2022)
Article
Agronomy
Hector Aaron Lee-Rangel, Anayeli Vazquez Valladolid, Heriberto Mendez-Cortes, Juan Carlos Garcia-Lopez, Gregorio Alvarez-Fuentes, Jose Alejandro Roque-Jimenez, Mario Alejandro Mejia-Delgadillo, Luis Octavio Negrete-Sanchez, Oswaldo Cifuentes-Lopez, Hugo Magdaleno Ramirez-Tobias
Summary: The study found that adding copra meal in lamb diet could decrease gas production volume and greenhouse gas emissions without affecting animal performance.
Article
Food Science & Technology
Zihan Zhang, Yucan Wu, Quanwei Liu, Guiping Zhao, Limin Wei, Chunhui Zhang, Feng Huang
Summary: This study investigated the effect of feeding copra meal on the flavor formation of Wenchang chicken. The results showed that the addition of copra meal changed the quality of the chicken and increased the types and contents of volatile compounds.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Veterinary Sciences
Frederic Vangroenweghe, Karl Poulsen, Olivier Thas
Summary: The study demonstrated that supplementing pig feed with exogenous heat-tolerant beta-mannanase can improve overall performance and reduce the occurrence of diarrhea in piglets. The group treated with the enzyme had better clinical scores and lower mortality rates compared to the control group.
PORCINE HEALTH MANAGEMENT
(2021)
Article
Biochemistry & Molecular Biology
Weiwei Wang, Rao Fu, Tao Dong, Qingyun Cao, Hui Ye, Changming Zhang, Zemin Dong, Dingyuan Feng, Jianjun Zuo
Summary: Oral administration of GGGM led to compromised growth performance, increased relative weight of spleen and epididymal fat, and elevated alpha 1-acid glycoprotein content in both serum and livers of mice. Additionally, GGGM treatment resulted in increased activities of intestinal lactic dehydrogenase and succinic dehydrogenase, as well as increased consumption of reducing sugar by intestinal epithelial cells and reduced cell viability. Intestinal inflammatory responses were triggered by GGGM treatment, as evidenced by increased expression of inflammatory cytokines TNF-alpha and IL-6 in both in vivo and in vitro experiments.
Article
Multidisciplinary Sciences
Witida Sathitkowitchai, Narissara Suratannon, Suttipun Keawsompong, Wanlapa Weerapakorn, Preecha Patumcharoenpol, Sunee Nitisinprasert, Massalin Nakphaichit
Summary: Consumption of copra meal hydrolysate (CMH), particularly at a daily dose of 3 g, can improve bowel movement, increase microbial diversity and richness, and promote the growth of beneficial bacteria, leading to potentially beneficial effects on gut health for healthy individuals.
Article
Biotechnology & Applied Microbiology
Beenish Sadaqat, Chong Sha, Parveen Fatemeh Rupani, Hongcheng Wang, Wanbing Zuo, Weilan Shao
Summary: The study identified a novel bifunctional mannanase and cellulase Man/Cel5B from the TM1752 gene in Tma, which showed exceptional hyperthermophilic characteristics and efficient substrate degradation capability, making it a promising candidate for applications in food, feed, and biofuel industries.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Wanphen Weil, Robert C. Weil, Suttipun Keawsompong, Klanarong Sriroth, Paul A. Seib, Yong-Cheng Shi
Summary: Pyrodextrins were prepared from acidified waxy and normal tapioca starches under different temperatures and times to investigate their in vitro digestibility and molecular structure. The results showed that pyrodextrin from waxy tapioca starch had higher levels of total and low-molecular weight indigestible carbohydrates compared to normal tapioca starch when prepared at higher temperatures and longer times. Furthermore, changes in molecular structure were observed, with waxy tapioca starch showing a greater tendency for thermal modification and increased non alpha-1,4/alpha-1,6 glucosidic linkages in the pyrodextrins with higher indigestible carbohydrate content.
CARBOHYDRATE POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Francis Ayimbila, Suttipun Keawsompong
Summary: Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides. Three types of crude polysaccharides extracted from L. squarrosulus showed differences in functional compositions and radical scavenging activities. LSP-CP and LSP-HP exhibited good ABTS scavenging abilities, while LSP-AP showed poor performance as an ABTS scavenger.
Article
Genetics & Heredity
Nachon Raethong, Massalin Nakphaichit, Narissara Suratannon, Witida Sathitkowitchai, Wanlapa Weerapakorn, Suttipun Keawsompong, Wanwipa Vongsangnak
Summary: The study analyzed the gut microbiome of Thai adults, identifying two dominant bacterial families and a significant number of genes associated with carbohydrate metabolism. The importance of Roseburia and Faecalibacterium in central carbon metabolism and butyrate biosynthesis was highlighted through bacterial community-wide metabolic functions. This initial study of shotgun metagenomics provides valuable insights into the gut microbiome of a Thai population in a developing Southeast Asian country.
Article
Multidisciplinary Sciences
Manuwat Tintarasara Na Ratchaseema, Lawan Kladsuwan, Laurent Soulard, Pratchya Swangmaneecharern, Prapat Punpee, Peeraya Klomsa-ard, Klanarong Sriroth, Suttipun Keawsompong
Summary: The research indicates that spraying BTH and SA can increase the levels of SA and total phenolic compounds, reduce the concentration of phytoplasma in plants, and induce an efficient SAR response to phytoplasma associated with SCWL disease.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Francis Ayimbila, Supatcharee Siriwong, Massalin Nakphaichit, Suttipun Keawsompong
Summary: This study examined the nutritional composition, digestion, and colonic fermentation of Lentinus squarrosulus powder (LP), finding that it has the potential to improve gut health.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Jurairat Rungruangsaphakun, Massalin Nakphaichit, Suttipun Keawsompong
Summary: This study investigated the optimal hydrolysis conditions for improving copra meal using mannanase from Bacillus circulans in a fed-batch setting. The effects of hydrolyzed copra meal (HCM) and conventional copra meal (CM) on simulated swine gastrointestinal digestion were also studied. The experimental results showed that fed-batch hydrolysis under optimal conditions resulted in higher reducing sugar and total oligosaccharide yield compared to batch hydrolysis. Mannanase also reduced the fiber content and caused significant degradation of copra meal microfibrils. The simulated digestion revealed that HCM was more easily digested than CM.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Witida Sathitkowitchai, Francis Ayimbila, Sunee Nitisinprasert, Suttipun Keawsompong
Summary: The impact of steam pretreatment and mannanase on the quality of palm kernel cake was investigated. Steam pretreatment increased protein content and decreased hemicellulose and lignin content in the cake. Mannanases from E. coli KMAN-3 and E. coli-Man6.7 showed the highest activity, and NT6.3 mannanase hydrolyzed steamed cake released the most reducing sugar, total sugar, and protein.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Witida Sathitkowitchai, Francis Ayimbila, Sunee Nitisinprasert, Suttipun Keawsompong
Summary: This study optimized the conditions for enzymatically hydrolyzing steamed palm kernel cake (PKC) to improve its functionality as a feedstuff. The results showed that the optimized conditions resulted in higher release of protein and carbohydrate, while the protein content decreased during pepsin digestion.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Francis Ayimbila, Phatcharin Prayoonthien, Tanapak Inyod, Dietmar Haltrich, Suttipun Keawsompong
Summary: This study investigated the effects of Macrocybe crassa (or Tricholoma crassum) powder on human gut microbiota and short-chain fatty acid production. The results showed that the powder significantly modulated the gut microbiota by increasing the relative abundances of beneficial bacteria and promoting the production of short-chain fatty acids. This research is important for filling the knowledge gap and revealing the significance of M. crassa in the pharmaceutical industry.
Article
Agriculture, Dairy & Animal Science
Witida Sathitkowitchai, Ponsit Sathapondecha, Pacharaporn Angthong, Yanee Srimarut, Yuwares Malila, Wuttichai Nakkongkam, Sage Chaiyapechara, Nitsara Karoonuthaisiri, Suttipun Keawsompong, Wanilada Rungrassamee
Summary: This study isolated two bacterial strains, Man26 and Man122, from shrimp intestines and found that they have the ability to produce mannanase, an enzyme that digests MOS and other mannan-rich materials. These strains can tolerate a wide pH range and their crude enzymes significantly increased the release of sugars and proteins from copra meal, palm kernel cake, and soybean meal. These findings suggest that these bacterial strains could potentially be used in animal feeds to improve the utilization of feedstuffs.
Article
Biotechnology & Applied Microbiology
Francis Ayimbila, Suttipun Keawsompong
Summary: Agro-waste and spent yeast are global environmental concerns, but they can produce bioactive mannan oligosaccharides (MOS) that have preventive and control effects against diseases. MOS can interact with body cells, induce immunity, influence enzyme activity, reduce oxidation, and modulate the microbiota. Research on the effects of MOS in humans is limited, highlighting the need for further investigation.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Biology
Amornthep Kingkaw, Nachon Raethong, Preecha Patumcharoenpol, Narissara Suratannon, Massalin Nakphaichit, Suttipun Keawsompong, Sittiruk Roytrakul, Wanwipa Vongsangnak
Summary: This study aimed to analyze the predominant species and metabolic routes involved in SCFAs production in the human gut microbiome after treatment with CMH. It was found that CMH may potentially be used as a prebiotic diet for modulating and maintaining the gut microbiome implicated in human health.
Article
Food Science & Technology
Nawapan Pongsapipatana, Wireeya Chawjiraphan, Suttipun Keawsompong, Sunee Nitisinprasert
Summary: This study investigated the production and functions of COPRA-MOS on bacterial aggregation activity and microbial changes in the simulated gastrointestinal tract. The results showed that COPRA-MOS had positive effects on the growth and autoaggregation activity of probiotics, while it had a negative effect on the autoaggregation activity of pathogens. In the simulated gastrointestinal model, COPRA-MOS was beneficial for cecal fermentation, while in the chicken simulation model, it had a positive effect on the population of Lactobacillus.
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
(2022)
Review
Nutrition & Dietetics
Francis Ayimbila, Suttipun Keawsompong
Summary: This review provides an overview of the viability of mushroom protein as a future protein alternative, emphasizing its nutritional value, quality, digestibility, and biological benefits. Mushroom proteins have a complete essential amino acid profile, meet dietary requirements, and offer economic advantages over animal and plant sources. They possess antioxidant, antitumor, ACE inhibitory, and antimicrobial properties that promote human health. Overall, mushroom proteins are inexpensive, high-quality alternatives that can be used as meat substitutes, pharmaceuticals, and treatments for malnutrition.
CURRENT NUTRITION REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Francis Ayimbila, Supatcharee Siriwong, Varaporn Chaiyama, Natthapach Srihanant, Suttipun Keawsompong
Summary: This study compared the functional composition and bioactivities of Ganoderma lucidum and Ganoderma neo-japonicum and found that G. lucidum exhibited higher protein and phenolic compound content, as well as better antioxidant and antibacterial properties.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2023)