Journal
GENES
Volume 11, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/genes11060601
Keywords
bta-miR-210; bovine; progenitor cells; intramuscular adipogenesis; WISP2
Categories
Funding
- National Natural Science Foundation of China [31672384]
- Cattle Breeding Innovative Research Team [ASTIPIAS03, ZDXT2018006]
- Program of National Beef Cattle and Yak Industrial Technology System [CARS-37]
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Intramuscular fat (IMF) is one of the major factors determining beef quality. IMF formation is influenced by multiple conditions including genetic background, age and nutrition. In our previous investigation, bta-miR-210 was found to be increased during adipogenesis using miRNA-seq. In this study, we validated the upregulation of bta-miR-210 in platelet-derived growth factor receptor alpha positive (PDGFR alpha(+)) progenitor cells during adipogenic differentiation in vitro. To investigate its role in adipogenesis, bta-miR-210 mimics were introduced into progenitor cells, which resulted in enhanced intracellular lipid accumulation. Accordingly, the expression of adipocyte-specific genes significantly increased in the bta-miR-210 mimic group compared to that in the negative control group (p< 0.01). Dual-luciferase reporter assays revealed thatWISP2is a target of bta-miR-210.WISP2knockdown enhanced adipogenesis. In conclusion, bta-miR-210 positively regulates the adipogenesis of PDGFR alpha(+)cells derived from bovine fetal muscle by targetingWISP2.
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