Journal
GENES
Volume 11, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/genes11040466
Keywords
clustered regularly interspaced short palindromic repeat; base editor; point mutation; genome editing; base conversion
Categories
Funding
- Agricultural Sciences and Technologies Innovation Program of the Chinese Academy of Agricultural Sciences
- National Science Foundation of China [31871597, 31872861]
Ask authors/readers for more resources
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9), a newly developed genome-editing tool, has revolutionized animal and plant genetics by facilitating modification of target genes. This simple, convenient base-editing technology was developed to improve the precision of genome editing. Base editors generate precise point mutations by permanent base conversion at a specific point, with very low levels of insertions and deletions. Different plant base editors have been established by fusing various nucleobase deaminases with Cas9, Cas13, or Cas12a (Cpf1), proteins. Adenine base editors can efficiently convert adenine (A) to guanine (G), whereas cytosine base editors can convert cytosine (C) to thymine (T) in the target region. RNA base editors can induce a base substitution of A to inosine (I) or C to uracil (U). In this review, we describe the precision of base editing systems and their revolutionary applications in plant science; we also discuss the limitations and future perspectives of this approach.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available