Review
Physiology
M. Caroline Muellenbroich, Allen Kelly, Corey Acker, Gil Bub, Tobias Bruegmann, Anna Di Bona, Emilia Entcheva, Cecilia Ferrantini, Peter Kohl, Stephan E. Lehnart, Marco Mongillo, Camilla Parmeggiani, Claudia Richter, Philipp Sasse, Tania Zaglia, Leonardo Sacconi, Godfrey L. Smith
Summary: Optical techniques have rapidly advanced the field of cellular electrophysiology, allowing for in-depth analysis of cardiac dynamics and opening new possibilities for clinical applications with the recent advent of optogenetics.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Prachi Umbarkar, Sultan Tousif, Anand P. Singh, Joshua C. Anderson, Qinkun Zhang, Michelle D. Tallquist, James Woodgett, Hind Lal
Summary: This study reveals the important role of cardiac fibroblast-GSK-3 alpha in myocardial fibrosis and heart failure, and identifies the molecular mechanism underlying this role through fibroblast kinome mapping. The results suggest that GSK-3 alpha plays a critical role in fibroblast transformation and fibrotic remodeling, independently of the canonical TGF-beta 1-SMAD3 pathway.
CIRCULATION RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Steven E. E. Wilson
Summary: Mesenchymal cells and mesenchymal progenitor bone marrow-derived fibrocytes play a crucial role in stromal fibrosis after corneal injury. They have both anti-fibrotic and pro-fibrotic functions, such as regulating the effects of transforming growth factor, modulating the entry of bone marrow-derived cells, promoting corneal epithelial healing, and contributing to the regeneration of basement membranes. The balance between these functions is important in determining the outcome of tissue fibrosis.
Article
Multidisciplinary Sciences
Patrick M. Boyle, Jinzhu Yu, Aleksandra Klimas, John C. Williams, Natalia A. Trayanova, Emilia Entcheva
Summary: Intercellular electrical coupling plays a crucial role in cellular communication, and OptoGap is a new contactless method for quantifying cell-cell coupling through selective illumination of non-excitable cells and optical sensing of excitable cell responses. Experimental validation in a two-dimensional setting shows the potential applicability of OptoGap to the integration of fibroblasts and non-transformed cardiomyocytes, while computational analysis confirms its sensitivity to cell-scale excitability.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Nicoletta Braidotti, Suet Nee Chen, Carlin S. Long, Dan Cojoc, Orfeo Sbaizero
Summary: This review presents recent discoveries regarding the role of the mechanosensitive ion channel Piezo1 in various diseases, particularly in tumor progression and cardiac mechanobiology. Based on recent findings, Piezo1 could be upregulated in cardiac fibroblasts due to mechanical stress and pro-inflammatory stimuli after myocardial injury, leading to fibrosis progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Deepak Ramanujam, Anna Patricia Schon, Christina Beck, Paula Vaccarello, Giulia Felician, Anne Dueck, Dena Esfandyari, Gunter Meister, Thomas Meitinger, Christian Schulz, Stefan Engelhardt
Summary: Recent research has shown that miR-21 is the most highly expressed microRNA in cardiac macrophages, playing a critical role in fibrosis and cardiac dysfunction. Furthermore, miR-21 regulates the polarization of macrophages towards an M1-like phenotype and promotes communication between macrophages and cardiac fibroblasts, leading to fibrotic remodeling.
Article
Cell Biology
Stephanie D. Burr, James A. Stewart
Summary: Heart failure is a common complication for individuals with type 2 diabetes mellitus, potentially caused by differentiation of fibroblasts to myofibroblasts associated with AGE/RAGE signaling.
Review
Pharmacology & Pharmacy
Kenneth K. Wu
Summary: Tissue fibrosis is a dynamic process associated with tissue inflammation, leading to debilitating human diseases. 5-methoxytryptophan (5-MTP) has shown significant efficacy in attenuating fibrosis in different organs and may serve as a promising lead compound for developing new anti-fibrotic drugs.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Judy R. Sayers, Paul R. Riley
Summary: The loss of heart muscle cells and associated vasculature following a heart attack leads to scar formation, pathological heart remodelling, and ultimately heart failure. Research on the roles of diverse cell lineages in heart development and regeneration calls for a broader combinatorial approach to heart regeneration.
CARDIOVASCULAR RESEARCH
(2021)
Article
Physiology
Aparna C. Sankarankutty, Joachim Greiner, Jean Bragard, Joseph R. Visker, Thirupura S. Shankar, Christos P. Kyriakopoulos, Stavros G. Drakos, Frank B. Sachse
Summary: The study explores the conduction model of HF tissues using microscopy, revealing the importance of tissue composition and fibrosis heterogeneity in HF. It also suggests that there are differences in conduction characteristics between HF patients with different etiologies.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Xuhan Liu, Yuan Liu, Bo Li, Lin Wang, Weihua Zhang
Summary: Desmin myopathy is a rare genetic disorder causing severe atypical cardiomyopathy and skeletal myopathy. Diagnosis is made through genetic testing and supplementary examinations. Early detection and multidisciplinary management are crucial for improving patient outcomes.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Nehal J. Patel, Drew M. Nassal, Daniel Gratz, Thomas J. Hund
Summary: Therapeutic targeting of STAT3 for CVD and arrhythmias presents unique challenges and opportunities, emphasizing the critical consideration of the multimodal and dynamic nature of STAT3 signaling. It will be beneficial to explore ways to maintain balanced STAT3 function rather than causing large-scale perturbations in STAT3 signaling.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2021)
Review
Biochemistry & Molecular Biology
Steven E. Wilson
Summary: Bowman's layer is an acellular layer in the anterior stroma of the cornea. It does not significantly contribute to mechanical stability or act as a barrier. However, it represents ongoing cytokine and growth factor-mediated interactions between corneal epithelial cells and stromal keratocytes. The destruction of Bowman's layer is associated with certain eye diseases and the development of fibrovascular tissue in the cornea.
Article
Multidisciplinary Sciences
Christopher O'Shea, James Winter, S. Nashitha Kabir, Molly O'Reilly, Simon P. Wells, Olivia Baines, Laura C. Sommerfeld, Joao Correia, Ming Lei, Paulus Kirchhof, Andrew P. Holmes, Larissa Fabritz, Kashif Rajpoot, Davor Pavlovic
Summary: Optical mapping is a widely used technique in pre-clinical cardiac research, allowing for efficient study of cardiac electrophysiology and the effects of interventions on arrhythmia.
Article
Medicine, General & Internal
Theresa Isabelle Wilhelm, Thorsten Lewalter, Johannes Fischer, Judith Reiser, Julia Werner, Christine Baumgartner, Lukas Gleirscher, Petra Hoppmann, Christian Kupatt, Klaus Tiemann, Clemens Jilek
Summary: In this study, ultra-high-density mapping was used to analyze the electrical anatomy of healthy pig hearts. The left chambers showed higher velocity values and voltage amplitudes compared to the right chambers. There was a slightly positive correlation between slow conduction velocities and low voltage amplitudes.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Matthias Imboden, Etienne de Coulon, Alexandre Poulin, Christian Dellenbach, Samuel Rosset, Herbert Shea, Stephan Rohr
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
S. H. Vermij, H. Abriel, J. P. Kucera
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2020)
Article
Neurosciences
Omar Alijevic, Olivier Bignucolo, Echrak Hichri, Zhong Peng, Jan P. Kucera, Stephan Kellenberger
FRONTIERS IN CELLULAR NEUROSCIENCE
(2020)
Article
Physiology
Sarah Helena Vermij, Hugues Abriel, Jan Pavel Kucera
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Multidisciplinary Sciences
Etienne de Coulon, Christian Dellenbach, Stephan Rohr
Summary: The LSSE system allows for comprehensive intracellular electrical recordings on cells undergoing strain events, providing stable conditions for studying mechanically activated ion currents. This system offers advanced insights into mechanosensitive ion channel function and allows for quantitative comparisons among different types of channels and cells.
Article
Biology
Sabrina Vullo, Nicolas Ambrosio, Jan P. Kucera, Olivier Bignucolo, Stephan Kellenberger
Summary: Acid-sensing ion channels (ASICs) play important roles in neuroscience, but their activation mechanism remains unclear. Research suggests that conformational changes in channel activation speed may be driven by local protonation events, indicating the existence of a pathway linking protonation sites to the channel pore.
Article
Neurosciences
Ena Ivanovic, Jan P. Kucera
Summary: It has been suggested that the localization of Na+ channel clusters in the perinexi of gap junction plaques is crucial for ephaptic coupling in cardiac tissue, which is greatly modulated by perinexal width.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Neurosciences
Seyma Nayir, Stephanie P. Lacour, Jan P. Kucera
Summary: Cardiomyocyte cultures exhibit spontaneous electrical and contractile activity similar to a natural cardiac pacemaker. This study investigates the effects of myosin inhibitor blebbistatin and stretch-activated channel blocker streptomycin on beating variability and stretch-induced changes in beat rate. The results suggest that active force generation plays a key role in the complexity of beating variability and the increase in beat rate upon mechanical deformation.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Cell Biology
Ena Ivanovic, Jan P. Kucera
Summary: Cardiac ephaptic coupling, mediated by negative electric potentials in intercalated discs, can be influenced by the convoluted structure of the discs. Concentric folds enhance ephaptic interactions, while radial folds attenuate ephaptic interactions.
Article
Biochemical Research Methods
Karoline Horgmo Jaeger, Ena Ivanovic, Jan P. Kucera, Aslak Tveito
Summary: The electrophysiology of cardiomyocytes relies on ion channels in cell membranes. Close to these channels, strong electrical and chemical gradients exist, but they are often ignored in mathematical models due to their limited extent. We solved the Poisson-Nernst-Planck equations in a tiny extracellular space between adjacent cells and observed the behavior of ion channels. Opening potassium channels results in a stationary solution with a Debye layer, while opening sodium channels generates a strong and transient electrochemical wave that can extend to neighboring cells, potentially explaining cardiac conduction even with low gap junctional coupling.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Neurosciences
Zoja Selimi, Jean-Sebastien Rougier, Hugues Abriel, Jan P. P. Kucera
Summary: Cardiac voltage-gated sodium (Na+) channels play a crucial role in myocardial electrical excitation. Single-channel recordings indicate that these channels exhibit functional interactions and coupled gating. However, the analysis of such recordings often involves manual interventions, leading to potential bias. In this study, an automated pipeline was developed to de-trend and idealize single-channel currents, revealing weak or no functional interactions in wild-type Na(v)1.5 channels.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Physiology
Echrak Hichri, Zoja Selimi, Jan P. Kucera
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Ange Maguy, Jan P. Kucera, Jonas P. Wepfer, Virginie Forest, Flavien Charpentier, Jin Li
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2020)