Article
Biochemistry & Molecular Biology
Sathish Kumar Marimuthu, Krishnanand Nagarajan, Sathish Kumar Perumal, Selvamani Palanisamy, Latha Subbiah
Summary: Host defense peptides or antimicrobial peptides (AMPs) are short sequences of amino acids with positive or negative charges that exhibit broad-spectrum antibacterial, antiviral, and antifungal activity. AMPs rich in tryptophan, arginine, and proline are considered promising sources for the development of next-generation antibiotics. This study analyzed and classified 2924 AMPs based on their amino acid sequences and investigated their structures and stability using modeling and simulations.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Pharmacology & Pharmacy
Tomislav Roncevic, Matko Males, Yogesh Sonavane, Filomena Guida, Sabrina Pacor, Alessandro Tossi, Larisa Zoranic
Summary: Kiadins are peptides that resemble diPGLa-H and have varying activity and selectivity against bacteria, as well as cytotoxicity against host cells, depending on the number and placement of glycine residues. Molecular dynamics simulations reveal that the conformational flexibility introduced by glycine substitutions affects peptide structure and interactions with model membranes.
Article
Microbiology
Meng Wang, Zifeng Deng, Yanmei Li, Keyong Xu, Yi Ma, Shang-Tian Yang, Jufang Wang
Summary: In this study, a novel antibacterial peptide named PEW300 was found to have strong antibacterial and antibiofilm activity against Pseudomonas aeruginosa. It was able to disperse mature biofilms and target extracellular DNA, leading to the death of the bacteria. PEW300 also exhibited multiple actions including destroying cell membrane integrity, inducing high levels of intracellular reactive oxygen species, and interacting with genomic DNA.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Huizhen Ma, Yong Feng, Qianqian Cao, Jing Jia, Muhammad Ali, Dilip Shah, Blake C. Meyers, Hai He, Yu Zhang
Summary: We analyzed the evolutionary pattern of cysteine-rich peptides (CRPs) and found that CRPs are widely distributed in plants. The copy number of CRP genes varied significantly across lineages and was associated with the plant ecotype, possibly due to their resistance to changing pathogenic environments. We also investigated the unique bi-domain CRPs and provided insights into their antimicrobial and symbiosis characteristics.
PLANT CELL REPORTS
(2023)
Article
Food Science & Technology
Jose J. L. Silva, Maria R. A. de Miranda, Pedro F. N. Souza
Summary: Fungal contamination is a major cause of food spoilage, posing serious risks to human health and economic losses. This review discusses the recent advances in using synthetic peptides as agents to address foodborne fungal resistance and their applications in food preservation.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sabrina Pacor, Monica Benincasa, Maria Valentina Musso, Lucija Krce, Ivica Aviani, Alberto Pallavicini, Marco Scocchi, Marco Gerdol, Mario Mardirossian
Summary: Bivalve mollusks have a strong innate immunity and produce antimicrobial peptides like myticalins to protect themselves from potentially pathogenic microorganisms in the marine environment. However, myticalins demonstrated membrane disruption and permeabilization effects on E. coli, indicating potential for antimicrobial applications but also showing cytotoxicity towards human cells.
Review
Chemistry, Medicinal
Komal Sharma, Krishna K. Sharma, Amit Mahindra, Naina Sehra, Nitin Bagra, Shams Aaghaz, Rajesh Parmar, Gajanan K. Rathod, Rahul Jain
Summary: Modified and synthetic alpha-amino acids have diverse applications, and histidine is one such amino acid that can be subjected to synthetic modifications. Modified histidines have various applications ranging from biological activities to catalysis, nanotechnology, and polymer chemistry. Histidine plays an important role in scientific research.
MEDICINAL RESEARCH REVIEWS
(2023)
Article
Dentistry, Oral Surgery & Medicine
John Yun Niu, Iris Xiaoxue Yin, William Ka Kei Wu, Quan-Li Li, May Lei Mei, Chun Hung Chu
Summary: This study successfully developed a novel dual-action peptide, GA-KR12, with antimicrobial and mineralising properties. Results showed that GA-KR12 exhibited good antimicrobial effects against cariogenic species and mineralised enamel.
JOURNAL OF DENTISTRY
(2021)
Article
Food Science & Technology
Ping Zeng, Lanhua Yi, Qipeng Cheng, Jun Liu, Sheng Chen, Kin-Fai Chan, Kwok-Yin Wong
Summary: The antimicrobial peptide zp65 shows promising potential as a food preservative due to its strong bactericidal effect and low cytotoxicity. Zp65 can easily bind to bacterial membrane and form alpha-helix structure in amphiphilic environments. The peptide has high proteolytic stability and strong binding affinity to lipopolysaccharide, suggesting its possible action target on the bacterial envelope.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Manuel Carrer, Josefine Eilso Nielsen, Henrique Musseli Cezar, Reidar Lund, Michele Cascella, Thereza A. Soares
Summary: We investigate the lipid transport properties of model lipid membranes in the presence of antimicrobial peptide indolicidin. Experimental techniques are used to compare with fully atomistic molecular dynamics simulations. The results show that lipid flip-flop dynamics is greatly accelerated upon peripheral binding of the peptides, even at low concentrations. Computer modeling explains the interplay between structural changes and lipid dynamics induced by peptides and proposes a universal mechanism for the mode of action of antimicrobial peptides.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Medicinal
Giovanni Andrea Vitale, Silvia Scarpato, Alfonso Mangoni, Maria Valeria D'Auria, Gerardo Della Sala, Donatella de Pascale
Summary: Two linear proline-rich peptides (1-2) with N-terminal pyroglutamate were isolated from the marine bacterium Microbacterium sp. V1 associated with the marine sponge Petrosia ficiformis collected in Ischia Island. An integrated, untargeted MS/MS-based molecular networking and cheminformatic approach was used to detect these peptides along with others (3-8). Through extensive analysis, the planar structure and stereochemistry of the peptides were determined. Peptides 1 and 2 exhibited antioxidant properties in the FRAP assay.
Article
Chemistry, Medicinal
Rikeshwer Prasad Dewangan, Devesh Pratap Verma, Neeraj Kumar Verma, Ankit Gupta, Garima Pant, Kalyan Mitra, Saman Habib, Jimut Kanti Ghosh
Summary: In this study, a new type of proline-rich peptide mimetic was designed and synthesized. These lipopeptides exhibited significant antimicrobial activity against resistant bacteria and demonstrated resistance against trypsin digestion. They also showed synergistic antimicrobial activity when combined with tested antibiotics. The lipopeptides were found to interact with the bacterial ribosome and inhibit protein synthesis. Furthermore, they demonstrated superior clearance of bacteria compared to a conventional antibiotic.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Wenyi Li, Feng Lin, Andrew Hung, Anders Barlow, Marc-Antoine Sani, Rita Paolini, William Singleton, James Holden, Mohammed Akhter Hossain, Frances Separovic, Neil M. O'Brien-Simpson, John D. Wade
Summary: Antimicrobial peptides (AMPs) are effective against drug-resistant bacteria and possess immunomodulatory activity. Dimerization of AMPs using bifunctional tethers enhances their antimicrobial activity, especially against multidrug-resistant bacteria.
Review
Biochemistry & Molecular Biology
Matthew Drayton, Julia P. Deisinger, Kevin C. Ludwig, Nigare Raheem, Anna Mueller, Tanja Schneider, Suzana K. Straus
Summary: The rapid rise of multidrug-resistant bacteria has raised concerns about the effectiveness of traditional antibiotics. Antimicrobial peptides, with their diverse mechanisms of actions, offer a promising solution to combat these pathogens. This review discusses the mechanisms of antibiotic resistance and the potential of antimicrobial peptides in providing a more robust treatment of bacterial infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Dries Wyers, Thanavit Jirapanjawat, John F. Quinn, Michael R. Whittaker, Chris Greening, Tanja Junkers
Summary: This study describes the synthesis of monodisperse guanylated oligomers through RAFT polymerization and flash chromatography. The antimicrobial activity of the oligomers was enhanced by modifying the chain end and introducing a hydrophobic tail. The ideal chain length for antimicrobial action was identified, allowing for the production of more selective antimicrobial peptide mimics.
Article
Biochemistry & Molecular Biology
Britta Seip, C. Axel Innis
JOURNAL OF MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
A. Carolin Seefeldt, Michael Graf, Natacha Perebaskine, Fabian Nguyen, Stefan Arenz, Mario Mardirossian, Marco Scocchi, Daniel N. Wilson, C. Axel Innis
NUCLEIC ACIDS RESEARCH
(2016)
Article
Multidisciplinary Sciences
Stefan Arenz, Lars V. Bock, Michael Graf, C. Axel Innis, Roland Beckmann, Helmut Grubmueller, Andrea C. Vaiana, Daniel N. Wilson
NATURE COMMUNICATIONS
(2016)
Review
Biochemistry & Molecular Biology
Michael Graf, Mario Mardirossian, Fabian Nguyen, A. Carolin Seefeldt, Gilles Guichard, Marco Scocchi, C. Axel Innis, Daniel N. Wilson
NATURAL PRODUCT REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Justine Charon, Aitor Manteca, C. Axel Innis
Article
Microbiology
Alba Herrero del Valle, Britta Seip, Inaki Cervera-Marzal, Guenael Sacheau, A. Carolin Seefeldt, C. Axel Innis
NATURE MICROBIOLOGY
(2020)
Correction
Microbiology
Alba Herrero del Valle, Britta Seip, Inaki Cervera-Marzal, Guenael Sacheau, A. Carolin Seefeldt, C. Axel Innis
NATURE MICROBIOLOGY
(2020)
Review
Microbiology
Alba Herrero del Valle, C. Axel Innis
FEMS MICROBIOLOGY REVIEWS
(2020)
Article
Multidisciplinary Sciences
Anne-Xander van der Stel, Emily R. Gordon, Arnab Sengupta, Allyson K. Martinez, Dorota Klepacki, Thomas N. Perry, Alba Herrero del Valle, Nora Vazquez-Laslop, Matthew S. Sachs, Luis R. Cruz-Vera, C. Axel Innis
Summary: The study identifies a mechanism where the TnaC-ribosome complex captures L-Trp to initiate termination arrest, with nascent TnaC preventing release factor 2 from adopting an active conformation. The relative rates of L-Trp binding and peptidyl-tRNA cleavage determine the tryptophan sensitivity of each variant. This reveals a strategy whereby a nascent peptide assists the ribosome in detecting a small metabolite.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Bertrand Beckert, Elodie C. Leroy, Shanmugapriya Sothiselvam, Lars V. Bock, Maxim S. Svetlov, Michael Graf, Stefan Arenz, Maha Abdelshahid, Britta Seip, Helmut Grubmuller, Alexander S. Mankin, C. Axel Innis, Nora Vazquez-Laslop, Daniel N. Wilson
Summary: Macrolides and ketolides antibiotics inhibit bacterial protein synthesis by interfering with the translation of specific subset of proteins. The study shows how these antibiotics interact with nascent polypeptide chain to induce translational arrest, providing insights into their mechanism of action. The structures of ribosomes arrested during the synthesis of specific sequence motifs by macrolides and ketolides reveal the basis for their less stringent sequence-specific action, which has implications for future development of improved antibiotics.
NATURE COMMUNICATIONS
(2021)
Review
Biochemical Research Methods
Aitor Manteca, Alejandra Gadea, David Van Assche, Pauline Cossard, Melanie Gillard-Bocquet, Thomas Beneyton, C. Axel Innis, Jean-Christophe Baret
Summary: Optimizing the properties of biological molecules is crucial for various industrial and medical applications. Directed evolution is a powerful technique for modifying biomolecules by mimicking natural evolution. Droplet-based microfluidic systems offer a high-throughput solution for enhancing efficiency and overcoming screening limitations.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biology
Britta Seip, Guenael Sacheau, Denis Dupuy, C. Axel Innis
LIFE SCIENCE ALLIANCE
(2018)
Meeting Abstract
Biochemistry & Molecular Biology
Christophe Andre, Natacha Perebaskine, A. Carolin Seefeldt, Stephanie Antunes, Fabian Nguyen, Celine Douat, Daniel N. Wilson, C. Axel Innis, Gilles Guichard
JOURNAL OF PEPTIDE SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Mario Mardirossian, Natacha Perebaskine, Monica Benincasa, Stefano Gambato, Sven Hofmann, Paul Huter, Claudia Mueller, Kai Hilpert, C. Axel Innis, Alessandro Tossi, Daniel N. Wilson
CELL CHEMICAL BIOLOGY
(2018)