Article
Multidisciplinary Sciences
Eirik G. Kommedal, Fredrikke Saether, Thomas Hahn, Vincent G. H. Eijsink
Summary: This study found that redox compounds naturally present in shed insect exoskeletons can harvest light energy to drive LPMO reactions and biomass conversion. The impact of light on LPMO activity depends on both light and exoskeleton dosage, and it is inhibited by a competing H2O2-consuming enzyme.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Polymer Science
Carolina Buruaga-Ramiro, Noelia Fernandez-Gandara, L. Veronica Cabanas-Romero, Susana V. Valenzuela, F. I. Javier Pastor, Pilar Diaz, Josefina Martinez
Summary: Enzymatic treatment was used to obtain bacterial cellulose nanocrystals, which exhibited higher thermal stability and were effective in coating pre-existing cellulosic materials to improve mechanical properties. The combination of LPMO oxidation and glycosyl hydrolase hydrolysis was key in producing nanocrystals from bacterial cellulose.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Agricultural Engineering
Silvia Magri, Gulsen Nazerian, Tiriana Segato, Antonielle Vieira Monclaro, Marco Zarattini, Fernando Segato, Igor Polikarpov, David Cannella
Summary: This study investigates the constraints posed by substrate ultrastructure on the activity of seven different AA9 LPMOs, revealing that substrate crystallinity and dry matter loading impact enzyme efficiency in a specific manner, with lower efficiency AA9s causing greater disruption to the crystal lattice.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Wa Gao, Hefeng Zhang, Tang Li, Jiu Ju, Haichuan Zhou, Xu Zong, Heng Yin
Summary: In this article, a photoelectrochemical bioreactor was built to convert cellulose into favorable chemicals using solar energy. By integrating a silicon photocathode with an AA9 lytic polysaccharide monooxygenase-based enzymatic system, the supply of reductants and H2O2 in LPMO catalysis was regulated, resulting in efficient and stable cellulose degradation.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Lei Ma, Guangqi Li, Hongming Xu, Zhiying Liu, Qun Wan, Dongyang Liu, Qirong Shen
Summary: This study identified a novel AA9 family protein cPMO2 derived from composting, which demonstrated superior binding capacity and broad substrate specificity in cellulose degradation.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Miesho Hadush Berhe, Xiangfei Song, Lishan Yao
Summary: The protein sequence encoding for an LPMO from Bacillus amyloliquefaciens (BaLPMO10A) was optimized through protein engineering to enhance its catalytic efficiency. BaLPMO10A exhibited hydrolysis activity against various substrates and showed increased production when combined with commercial cellulase. Additionally, the engineered BaLPMO10A exhibited enhanced thermostability. This study provides a better tool for cellulose depolymerization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Liangkun Long, Lu Sun, Dafan Ding, Kaixiang Chen, Qunying Lin, Shaojun Ding
Summary: The two genes encoding lytic polysaccharide monooxygenases (LPMOs) CsLPMO9A and CsLPMO9B were successfully cloned and expressed in Komagataella phaffii. The two enzymes showed synergistic effects with a commercial cellulase cocktail and demonstrated promising potential in industrial bioconversion of lignocellulosic biomass by increasing the release of glucose, cellobiose, and xylose from the substrate.
PROCESS BIOCHEMISTRY
(2021)
Article
Chemistry, Applied
Fei Li, Xuejiao Sun, Wen Yu, Chengcheng Shi, Xiaoyu Zhang, Hongbo Yu, Fuying Ma
Summary: The LPMO from Pleurotus ostreatus efficiently depolymerizes KGM to produce oligosaccharides, which, when used as a carbon source, significantly promote the growth of probiotic bacteria and the production of SCFAs, leading to improved gastrointestinal health.
CARBOHYDRATE POLYMERS
(2021)
Article
Agriculture, Multidisciplinary
Fei Li, Ruijian Shao, Yingzheng Mao, Wen Yu, Hongbo Yu
Summary: In this study, LPMOs were found to efficiently supply H2O2 to DyPs for chitosan functionalization using GA as substrate, achieving a higher grafting ratio than reactions with exogenous H2O2. The GA-chitosan conjugates exhibited lower thermal stability and higher antioxidant activity compared to chitosan. This research provides a green and high-efficiency method for enzymatic cascade reactions in chitosan functionalization with potential applications in H2O2-dependent biocatalytic oxidation reactions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Josman Velasco, Ana Gabriela V. Sepulchro, Paula M. R. Higasi, Vanessa O. A. Pellegrini, David Cannella, Leandro Cristante de Oliveira, Igor Polikarpov, Fernando Segato
Summary: This study reports the functional characterization and structural modeling of a novel LPMO from Aspergillus fumigatus. The enzyme is capable of enhancing cellulose deconstruction through an oxidative mechanism and can be activated by light. The study also reveals a collaborative effect between a chemical reducing agent and light-induced electron transfer systems in changing the LPMO activity.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Review
Plant Sciences
Theruvothu Madathil Vandhana, Jean-Lou Reyre, Dangudubiyyam Sushmaa, Jean-Guy Berrin, Bastien Bissaro, Jogi Madhuprakash
Summary: Lytic polysaccharide monooxygenases (LPMOs) are a class of enzymes that can oxidatively cleave glycosidic bonds in carbohydrate polymers, facilitating the depolymerisation of recalcitrant biomasses. In addition to their role in biomass conversion, LPMOs may also play significant roles in fungal and oomycete plant pathogenesis, as well as in mutualistic/commensalism symbiosis. They could also be important in microbial pathogenesis and (micro)organism development.
Article
Engineering, Chemical
Pedro Ricardo Vieira Hamann, Milena Moreira Vacilotto, Fernando Segato, Igor Polikarpov
Summary: In this study, a new LPMO from Aspergillus fumigatus was biochemically characterized, showing higher efficiency against amorphous cellulose and pH-dependent regioselectivity. Photoactivation experiments demonstrated that it can be activated by light-stimulated chlorophyllin. Moreover, it can boost cellulose depolymerization in combination with other enzymes.
Review
Biotechnology & Applied Microbiology
Hongyu Zhang, Zixuan Zhou, Tingting Lou, Rong Xiang, Deguang Zhang, Danyun Wang, Suying Wang
Summary: This paper reviews the research progress of AA10 LPMOs' heterologous expression systems, introduces the strategies for constructing diversified heterologous expression systems, discusses the effects of different expression systems on the soluble expression of LPMOs, and explores the future development direction of LPMOs' heterologous expression systems. The broad application prospect of LPMOs in biomass conversion and biofuel industry is also prospected.
FERMENTATION-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Fei Li, Honglu Zhao, Yuxin Liu, Jiaqi Zhang, Hongbo Yu
Summary: This study found that the lpmo gene Sclpmo10G in Streptomyces coelicolor A3(2) strain is strongly induced by chitin, suggesting that ScLPMO10G may play a role in chitin biodegradation and have potential applications in biorefineries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Olav A. Hegnar, Heidi Ostby, Dejan M. Petrovic, Lisbeth Olsson, Aniko Varnai, Vincent G. H. Eijsink
Summary: Certain AA9 LPMOs were found to preferentially cleave xylan when acting on a cellulose-glucuronoxylan mixture, and this ability is due to protein evolution resulting in specific structural features. These findings strengthen the idea that the vast arsenal of AA9 LPMOs in certain fungal species provides functional versatility and may have evolved to promote oxidative depolymerization of a wide variety of recalcitrant, copolymeric plant polysaccharide structures.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laura Doblas, Per M. Hagglund, Eduardo Fuentes-Lemus, Michael J. Davies
Summary: This study investigates the modifications of proteins during milk processing and storage, with a focus on oxidation and crosslinking. The research reveals the formation and stability of crosslinks generated by the addition of Cys residues to quinones produced from the oxidation of Tyr residues. The findings provide insights into a novel mechanism of protein crosslink formation and information on the stability of these species, which could serve as markers for protein quality.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Plant Sciences
Vladimir Novoderezhkin, Roberta Croce
Summary: Lhca1 is a pigment-protein complex in the outer antenna of plant Photosystem I, and it plays a crucial role in delivering energy to the core. The unique energy distribution of Lhca1 allows for direct energy transfer to the core and coupling with the neighboring Lhca4 antenna for efficient excitation energy transfer.
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Microbiology
Julie A. Z. Zedler, Marlene Michel, Georg Pohnert, David A. A. Russo
Summary: This study describes a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which exhibits a fast-sedimenting phenotype. The Group 3 alternative sigma factor F was found to play a key role in cell sedimentation through whole genome sequencing and gene deletion. Changes in cell surface structures and an increase in released polysaccharides were identified as the factors contributing to the fast-sedimenting phenotype. This research sheds light on the transition from planktonic to benthic forms and provides genetic targets for the development of fast-sedimenting strains for cost-effective cyanobacterial harvesting at scale.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Plant Sciences
Chen Hu, Vincenzo Mascoli, Eduard Elias, Roberta Croce
Summary: CAM plants have a unique photosynthesis pathway and morphological features, making them more resistant to drought compared to C3 plants. However, little is known about the photosynthetic machinery of CAM plants. In this study, the photosynthetic apparatus of Tillandsia flabellate, an obligatory CAM plant, was investigated using biochemical and biophysical methods. The results showed that most of the Photosystems super- and sub-complexes have properties similar to those of Arabidopsis, with the main difference being the binding of extra LHCI in Tillandsia PSI-LHCI complexes. The functional measurements revealed that the PSI/PSII ratio was low, and the antenna size of both PSI and PSII was small. Despite a reduction in non-photochemical quenching (NPQ), no significant changes were observed in the composition of the photosystems, and PSII efficiency remained high even after 30-day water deficiency.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Chemistry, Physical
Peicheng Sun, Zhiyu Huang, Sanchari Banerjee, Marco A. S. Kadowaki, Romy J. Veersma, Silvia Magri, Roelant Hilgers, Sebastian J. Muderspach, Christophe V. F. P. Laurent, Roland Ludwig, David Cannella, Leila Lo Leggio, Willem J. H. van Berkel, Mirjam A. Kabel
Summary: In this study, two new fungal oxidoreductases from the AA16 family were characterized. These enzymes were found to be capable of producing H2O2 by oxidizing low-molecular-weight reductants. The interplay between AA16 proteins and MtLPMO9 proteins significantly enhanced cellulose degradation. These findings provide new insights into the functions of copper-dependent enzymes and the interplay of oxidative enzymes within fungal systems to degrade lignocellulose.
Article
Multidisciplinary Sciences
Christopher J. Gisriel, Eduard Elias, Gaozhong Shen, Nathan T. Soulier, David A. Flesher, M. R. Gunner, Gary W. Brudvig, Roberta Croce, Donald A. Bryant
Summary: To adapt to low-light environments, some cyanobacteria express a variant of the light-harvesting protein allophycocyanin (AP) that strongly absorbs far-red light (FRL). The structure-function relationship of this FRL-AP complex, expressed during acclimation to low light, was revealed using cryo-electron microscopy and time-resolved absorption spectroscopy. FRL-AP forms helical nanotubes instead of typical toroids due to altered domain geometry within each subunit. This study expands the understanding of light-harvesting proteins in nature and highlights the trade-off between resource accessibility and efficiency achieved through biological plasticity.
Review
Multidisciplinary Sciences
Giovanni D. Barone, Tomislav Cernava, Jorg Ullmann, Jing Liu, Elia Lio, Anna T. Germann, Andreas Nakielski, David A. Russo, Ted Chavkin, Kirstin Knufmann, Farida Tripodi, Paola Coccetti, Francesco Secundo, Pengcheng Fu, Brian Pfleger, Ilka M. Axmann, Peter Lindblad
Summary: The growing use of photosynthetic microorganisms for food and food-related applications is driven by increasing consumer acceptance, demand for sustainable and eco-friendly food sources, and global economic concern. Microalgae and cyanobacteria offer potential as sustainable sources of protein and fatty acids. Industrial applications of these microorganisms create new jobs and support sustainable development. However, there are still knowledge gaps in their optimized production and utilization. This comprehensive assessment highlights their potential and aims to stimulate further research.
Article
Biochemistry & Molecular Biology
Qing Gao, Katarzyna Grzyb, Luke F. Gamon, Peter R. Ogilby, Tomasz Pedzinski, Michael J. Davies
Summary: Disulfide bonds are critical for protein structure and function, and their modification can lead to loss of structure and function. This study investigates the oxidation of disulfide bonds by singlet oxygen, showing selective oxidation and variation in reaction rates and product formation. These findings suggest that certain disulfides, including those in proteins, may undergo detrimental modifications that affect protein structure and function.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Juan Sebastian Reyes, Eduardo Fuentes-Lemus, Jefferson Romero, Felipe Arenas, Angelica Fierro, Michael J. Davies, Camilo Lopez-Alarcon
Summary: 6-phosphogluconolactonase (6PGL) is an enzyme involved in the pentose phosphate pathway (PPP) and is susceptible to oxidative inactivation. This study investigates the oxidation of Escherichia coli 6PGL by peroxyl radicals and its effect on enzymatic activity and NADPH generation. The results show that while monomeric 6PGL is resistant to oxidative inactivation, protein aggregation and oxidation of specific residues occur, leading to diminished NADPH generation. These findings contribute to our understanding of the oxidative stability of 6PGL compared to other PPP enzymes.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Guilherme T. Voss, Michael J. Davies, Carl H. Schiesser, Renata L. de Oliveira, Andresa B. Nornberg, Angelita M. Barcellos, Cristiane Luchese, Ethel A. Wilhelm
Summary: This study investigated the use of 1,4-anhydro-4-seleno-D-talitol (SeTal) in gelatin and alginate (Gel-Alg) polymeric films to improve the treatment and attenuation of atopic dermatitis (AD)-like symptoms in mice. The films were able to retain and release SeTal in a controlled manner and effectively attenuated disease symptoms. Moreover, the loaded films showed superior efficiency compared to hydrocortisone (HC) cream, a traditional AD treatment. Overall, incorporating SeTal in biopolymeric films provides a promising alternative for the long-term treatment of AD-type skin diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
Angie C. Forero-Giron, Denis Fuentealba, Nory Marino-Ocampo, Soledad Gutierrez-Oliva, Barbara Herrera, Alejandro Toro-Labbe, Eduardo Fuentes-Lemus, Michael J. Davies, Margarita E. Aliaga, Camilo Lopez-Alarcon
Summary: The stable inclusion complex of AAPH with CB7 can generate high yields of peroxyl radicals, making it useful for studying these species in various fields such as material, environmental, and biomedical sciences.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Biochemistry & Molecular Biology
Joao M. Anghinoni, Paloma T. Birmann, Marcia J. da Rocha, Caroline S. Gomes, Michael J. Davies, Cesar A. Bruening, Lucielli Savegnago, Eder J. Lenardao
Summary: In this review, the synthesis and bioassays of organic selenium compounds with antioxidant activity in the past five years are discussed, presenting the most active compounds in each series.
Article
Plant Sciences
Chen Hu, Eduard Elias, Wojciech J. Nawrocki, Roberta Croce
Summary: Drought has significant effects on the photosynthetic apparatus of plants, leading to the disassembly and degradation of PSII supercomplexes and cores, as well as reduced antenna size and disassembly of PSI-LHCI supercomplexes. Despite no changes in PSI core protein content, the functionality of PSI is severely affected, suggesting the accumulation of nonfunctional PSI complexes. The operative quantum efficiency of PSII can be used as an early detection parameter for drought stress.
Article
Plant Sciences
Xin Liu, Wojciech J. Nawrocki, Roberta Croce
Summary: This study reveals the crucial role of LHCBM1 in protecting photosynthetic organisms from excess light, potentially enhancing the function of LHCSR3 in the process of nonphotochemical quenching.
Article
Multidisciplinary Sciences
Xiaojie Pang, Wojciech J. Nawrocki, Pierre Cardol, Mengyuan Zheng, Jingjing Jiang, Yuan Fang, Wenqiang Yang, Roberta Croce, Lijin Tian
Summary: Weak acids produced during fermentation can suppress both photosynthesis and aerobic respiration. This effect is widespread among photosynthetic organisms and helps algae adapt to changing environmental conditions.
NATURE COMMUNICATIONS
(2023)