Article
Environmental Sciences
Marija Mrdakovic, Aleksandra Filipovic, Larisa Ilijin, Anja Grcic, Dragana Matic, Milena Vlahovic, Dajana Todorovic, Vesna Peric-Mataruga
Summary: The response of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 was analyzed in the midgut, midgut tissue, and brain of Lymantria dispar L. and Euproctis chrysorrhoea L. larvae after chronic exposure to dietary fluoranthene. Carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae with a lower fluoranthene concentration. The different expression patterns of isoforms in larvae of both species contribute to efficient carboxylesterase activity as part of their defense mechanisms. The increased concentration of Hsp70 in the brain of L. dispar larvae indicates a response to the proteotoxic effects of a lower fluoranthene concentration. The decreased Hsp70 levels in the brain of E. chrysorrhoea larvae suggest the induction of other defense mechanisms.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Entomology
Michael E. Sparks, Yi-Ming Wang, Juan Shi, Robert L. Harrison
Summary: The spongy moth virus Lymantria dispar iflavirus 1 (LdIV1) was found in female moths from both the USA and China populations. The genomes of LdIV1 variants were compared and divided into clades based on the geographic origin of their hosts. The presence of LdIV1 RNA was detected at high levels in all samples, showing a wide range of LdIV1 reads in the sequenced volume.
Article
Environmental Sciences
Anja Grcic, Larisa Ilijin, Aleksandra Filipovic, Dragana Matic, Marija Mrdakovic, Dajana Todorovic, Milena Vlahovic, Vesna Peric-Mataruga
Summary: Global temperature increases have strong effects on insects, including phytophagous pest larvae of Lymantria dispar. This study investigated the effects of short-term heat stress on digestive enzymes and metabolic macromolecules in the larvae, revealing a higher sensitivity in populations adapted to pollution. The results highlight the substantial impact of sublethal temperature increases on the digestive and metabolic processes of L. dispar larvae.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Anja Grcic, Larisa Ilijin, Dragana Matic, Aleksandra Filipovic, Marija Mrdakovic, Dajana Todorovic, Vesna Peric-Mataruga
Summary: The study examined the physiological responses of Lymantria dispar larvae to chronic exposure to B[a]P and found that larvae from different origins showed varying reactions to the pollutant. It was observed that the physiological parameters of the larvae exhibited origin-dependent responses to the long-term presence of B[a]P, highlighting the importance of considering population variability in ecotoxicological research.
ENVIRONMENTAL POLLUTION
(2021)
Article
Entomology
Irina Belousova, Sergey Pavlushin, Anna Subbotina, Natalya Rudneva, Vyacheslav Martemyanov
Summary: The innate immunity of insects has been widely studied, but differences in immunity between the sexes of larvae insects remain largely unstudied. This study compared the antibacterial activity in females and males of Lymantria dispar larvae and found that the activation of antibacterial activity in the midgut was only observed in females after infection by the entomopathogenic bacterium. This suggests that sex differences in immunity can have important effects even before sexual differentiation at adulthood.
JOURNAL OF INSECT SCIENCE
(2021)
Article
Medicine, Research & Experimental
Yujing Du, Zhao Chen, Ping Yan, Chunli Zhang, Xiaojiang Duan, Xueqi Chen, Meng Liu, Lei Kang, Xing Yang, Yan Fan, Jianhua Zhang, Rongfu Wang
Summary: The study identified heat shock protein 70 (HSP70) as the binding target for RRL, revealing a potential interaction between RRL and the nucleotide-binding domain (NBD) of HSP70. Clathrin-dependent endocytosis and macropinocytosis were suggested as vital internalization mechanisms of RRL. In vivo, Tc-99m-MAG3-RRL demonstrated satisfactory tumor accumulation and targeting, providing a promising probe for visualizing overexpressed HSP70 tumor sections.
MOLECULAR PHARMACEUTICS
(2021)
Article
Medicine, Legal
E. Doberentz, A. Wegner, L. Rochlitzer, B. Madea, J. Ulbricht
Summary: In cases of fatal hemorrhage, there is no induction of extensive Hsp27 or 70 expression, indicating that fatal blood loss does not cause cellular stress.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Peixu Zhao, Christopher Rensing, Dun Wang
Summary: Our study demonstrated that symbiotic bacteria regulate the immune response in Lymantria dispar after infection with L. dispar Nucleopolyhedrovirus (LdMNPV) by changing the proportion of Gram-positive and Gram-negative bacterial community. The immune deficiency pathway was immediately activated after oral infection, promoting the secretion of antimicrobial peptides, while the abundance of the Gram-negative bacterial community increased. The Toll pathway showed a different regulation pattern compared to the Imd pathway, but its expression remained positively correlated to the abundance of Gram-positive bacteria. This finding suggests that the ratio of Gram-negative to Gram-positive bacteria influences the immune response in LdMNPV-infected larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yun-Ze Chen, Tao Li, Jing Yang, Qi-Meng Li, Guo-Cai Zhang, Jie Zhang
Summary: This study investigated the effects of carvacrol on Lymantria dispar larvae. The results showed that carvacrol treatment induced the production of antifeedants in larvae, leading to growth inhibition and death. Further analyses revealed that carvacrol affected the metabolism of xenobiotics, carbohydrates, and lipids in larvae. Additionally, carvacrol altered the expression levels of detoxification genes, including cytochrome P450s, esterases, glutathione peroxidase, UDP-glycosyltransferase, and heat shock proteins. These findings provide insights into the insecticidal mechanism of carvacrol.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Li-rong Yan, Shi-xuan Shen, Ang Wang, Han-xi Ding, Ying-nan Liu, Yuan Yuan, Qian Xu
Summary: The study showed differential expression of heat shock proteins (HSPs) in various cancers, with HSP expression levels generally associated with prognosis in multiple cancer types. While HSP gene mutations were generally linked to poor prognosis in cancers, copy number variations of HSPs had varying impacts on survival status in different cancers. HSPs were found to be involved in the activation and inhibition of multiple cancer-related pathways and closely correlated with immune cell infiltrations in different cancer types.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Larissa Smulders, Rachel Altman, Carolina Briseno, Alireza Saatchi, Leslie Wallace, Maha AlSebaye, Robert Stahelin, Nikolas Nikolaidis
Summary: HSPA1A is a molecular chaperone that regulates the survival of stressed and cancer cells. It localizes to the plasma membrane of stressed and tumor cells and interacts with specific lipids, playing a role in immunomodulatory functions and rendering tumors resistant to treatment. This study reveals that the surface presentation of HSPA1A is a multifaceted lipid-driven phenomenon controlled by binding to endosomal and plasma membrane lipids.
Article
Biochemistry & Molecular Biology
Chenshu Zhang, Peng Liu, Lili Sun, Chuanwang Cao
Summary: This study conducted an integrated analysis of miRNA and mRNA in Lymantria dispar larvae treated with cyantraniliprole, and identified 23 differentially expressed miRNAs (DEMs) and 432 differentially expressed genes (DEGs) related to cyantraniliprole tolerance. Among them, two novel miRNAs (novel-miR-4 and mmu-miR-3475-3p) were found to regulate the response of L. dispar to cyantraniliprole stress by targeting detoxification-related genes. This research provides valuable transcriptomic information for understanding insect tolerance mechanisms and developing new insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Entomology
Yiming Wang, Robert L. Harrison, Juan Shi
Summary: The study compared the effects of varying larval density on European gypsy moth and Asian gypsy moth populations, finding that all populations displayed optimal larval survival, pupation, and emergence rates at an intermediate larval density. While differences were observed among populations in terms of larval development, survival, and emergence, the study suggests that density-dependent effects on the development of different gypsy moth populations and subspecies follow similar trends.
Article
Medicine, Research & Experimental
Jiao Ge, Yan Liu
Summary: This study investigated the serum expression of HSP-70 in children with ITP and healthy controls, and found that the expression of HSP-70 is associated with the progression and severity of childhood ITP.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2022)
Article
Immunology
Stefan Tukaj, Jagoda Mantej, Michal Sobala, Katarzyna Potrykus, Zbigniew Tukaj, Detlef Zillikens, Ralf J. Ludwig, Katja Bieber, Michael Kasperkiewicz
Summary: The study found that Hsp70 immunization and anti-Hsp70 antibody treatment can decrease disease severity in a psoriasis-like skin inflammation mouse model by affecting T cell populations. This suggests that Hsp70 may be a promising therapeutic target for psoriasis and other autoimmune dermatoses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Larisa Ilijin, Anja Grcic, Marija Mrdakovic, Milena Vlahovic, Aleksandra Filipovic, Dragana Matic, Vesna Peric Mataruga
Summary: This paper investigates the effects of increased environmental temperature on the relative growth rate and developmental time of Lymantria dispar larvae originating from polluted and unpolluted forests. It also examines the impact of induced thermotolerance on antioxidative and detoxifying enzyme activities in larvae from different forest origins. The study suggests that certain enzyme activities could serve as potential biomarkers for thermal stress in L. dispar larvae.
JOURNAL OF THERMAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dragana Matic, Milena Vlahovic, Larisa Ilijin, Anja Grcic, Aleksandra Filipovic, Dajana Todorovic, Vesna Peric-Mataruga
Summary: The activities and isoform patterns of proteases and acid phosphatases in Lymantria dispar larvae can be used as biomarkers for cadmium exposure monitoring. Non-lysosomal ACP activity, ACP isoforms 4 and 5, and the IBR index are promising indicators of cadmium presence. Population sensitivity to cadmium exposure varied, with larvae from unpolluted areas showing more sensitivity compared to those from contaminated sites.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aleksandra Filipovic, Marija Mrdakovic, Larisa Ilijin, Anja Grcic, Dragana Matic, Dajana Todorovic, Milena Vlahovic, Vesna Peric-Mataruga
Summary: Fluoranthene, a polycyclic aromatic hydrocarbon, affects the digestive enzyme activities, growth rates, and isoform expressions in larvae of L. dispar and E. chrysorrhoea, highlighting the significance of physiological changes and fitness-related traits under pollutant stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Nikola Vesovic, Marija Nenadic, Marina Sokovic, Ana Ciric, Ljubodrag Vujisic, Marina Todosijevic, Natasa Stevanovic, Vesna Peric-Mataruga, Larisa Ilijin, Srecko Curcic
Summary: The chemical composition and antimicrobial potency of the pygidial gland secretion of the blue ground beetle were studied. Methacrylic and angelic acids were identified as the dominant chemicals in the secretion. The secretion exhibited antimicrobial action against certain bacteria and all tested micromycetes, with the strongest antifungal effect observed against Penicillium ochrochloron.
Article
Multidisciplinary Sciences
Larisa Ilijin, Anja Grcic, Marija Mrdakovic, Milena Vlahovic, Dajana Todorovic, Aleksandra Filipovic, Dragana Matic, Vesna Peric Mataruga
Summary: This study compared the effects of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae. The results showed that alkaline phosphatases activity was mainly influenced by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was significantly influenced by temperature and population, under both individual and combined effects. These findings suggest that brain tissue hsp70 concentration and alkaline phosphatases activity can be considered as a biomarker battery for thermal stress in L. dispar larvae as a bioindicator species.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Anja Grcic, Larisa Ilijin, Aleksandra Filipovic, Dragana Matic, Marija Mrdakovic, Dajana Todorovic, Milena Vlahovic, Vesna Peric-Mataruga
Summary: Global temperature increases have strong effects on insects, including phytophagous pest larvae of Lymantria dispar. This study investigated the effects of short-term heat stress on digestive enzymes and metabolic macromolecules in the larvae, revealing a higher sensitivity in populations adapted to pollution. The results highlight the substantial impact of sublethal temperature increases on the digestive and metabolic processes of L. dispar larvae.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Dragana Matic, Milena Vlahovic, Anja Grcic, Aleksandra Filipovic, Larisa Ilijin, Marija Mrdakovic, Jelena Mutic, Sladana Durdic, Vesna Peric-Mataruga
Summary: Long-term exposure to pollution may enhance the ability of populations to cope with environmental stress. This study compared the responses of two Lymantria dispar populations living in unpolluted and polluted forests to cadmium exposure. The results showed that larvae from the polluted population exhibited higher SOD activity and increased Hsp70 expression compared to larvae from the unpolluted population under control conditions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
