Review
Food Science & Technology
Pedro Reis Costa, Catarina Churro, Susana Margarida Rodrigues, Barbara Frazao, Miguel Barbosa, Lia Godinho, Lucia Solino, Viriato Timoteo, Neide Gouveia
Summary: This study reviews the occurrence of ciguatera cases in the Madeira Archipelago since its first detection in 2008, discusses the implemented risk management strategy, and provides a summary of the available data on the bioaccumulation of ciguatoxins (CTX) in the marine food web.
Review
Food Science & Technology
Da-Zhi Wang, Ye-Hong Xin, Ming-Hua Wang
Summary: Gambierdiscus, a widely distributed dinoflagellate genus in tropical and subtropical regions, produces potent toxins that pose a significant threat to human health and the environment. The expansion of CFP worldwide in recent years, due to human activities and global climate change, has resulted in increased human illness, greater public health impact, and larger economic losses. This review summarizes recent advances in the field of Gambierdiscus and its associated toxins, and provides future perspectives on this important issue.
Article
Food Science & Technology
Pedro Reis Costa, Pablo Estevez, Lucia Solino, David Castro, Susana Margarida Rodrigues, Viriato Timoteo, Jose Manuel Leao-Martins, Carolina Santos, Neide Gouveia, Jorge Diogene, Ana Gago-Martinez
Summary: The study found high levels of ciguatoxins in fish from the coastal food webs of the Selvagens Islands, especially in species like zebra seabream and barred hogfish. LC-MS/MS analysis confirmed C-CTX1 as the main toxin, but discrepancies between CBA and LC-MS/MS results in some species suggest the presence of fish metabolic products.
Review
Marine & Freshwater Biology
M. Chinain, C. Gatti Howell, M. Roue, A. Ung, K. Henry, T. Revel, P. Cruchet, J. Viallon, H. T. Darius
Summary: Ciguatera Poisoning (CP) is a prevalent seafood poisoning in French Polynesia, caused by seafood contaminated with ciguatoxins produced by Gambierdiscus. The toxin production of Gambierdiscus varies considerably in the natural environment, and certain areas represent biodiversity hotspots. G. polynesiensis is suggested as the primary source of CTXs in local ciguateric environments. Both herbivores and carnivores have high prevalence of ciguatoxic fish. While CP cases seem to decline overall, some islands still have dangerously high incidence rates. Effective risk management strategies should account for the cultural impact of CP on local communities.
Article
Chemistry, Medicinal
Sandra Raposo-Garcia, Alejandro Cao, Celia Costas, M. Carmen Louzao, Natalia Vilarino, Carmen Vale, Luis M. Botana
Summary: The expanding microorganisms producing ciguatoxins have raised significant concerns about ciguatera fish poisoning (CF), necessitating the development of a reliable and rapid ciguatoxin detection method to ensure food safety. This study evaluated various cell lines for CTX detection and revealed the limitations of the N2a cell line due to low availability of sodium channels and significant O/V damage. The HEK293 Nav cell line, expressing the alpha 1.6 subunit of sodium channels, proved to be sensitive to ciguatoxin without sensitization with O/V, providing an alternative for sodium channel-targeting compound detection.
Article
Marine & Freshwater Biology
Hiroshi Funaki, Chetan Chandrakant Gaonkar, Takafumi Kataoka, Tomohiro Nishimura, Kouki Tanaka, Ippei Yanagida, Shouta Abe, Haruo Yamaguchi, Keizo Nagasaki, Masao Adachi
Summary: This study investigated the distribution of Gambierdiscus species/phylotypes in Japan using high throughput sequencing, revealing the presence of new species/phylo-types as well as previously reported ones. The results highlight the importance of understanding the growth characteristics and abundance of these species/phylo-types to better understand the mechanism of ciguatera poisoning outbreaks in Japan.
Article
Environmental Sciences
Astrid Spielmeyer, Christopher R. Loeffler, Oliver Kappenstein
Summary: This study conducted an in-depth investigation using a semi-targeted LC-MS/MS approach to analyze tissue samples of Lutjanus bohar from the Indian Ocean and Pacific Ocean. The samples revealed a complex CTX contaminant profile, with the presence of several congeners of the CTX3C-group, which have a strong association with ciguatera poisoning. Furthermore, there were no distinguishable differences in toxin profile among individual samples or among the different oceanic capture regions, suggesting either a species-specific CTX metabolism or the emergence of an interoceanic CTX toxin profile.
FRONTIERS IN MARINE SCIENCE
(2022)
Review
Marine & Freshwater Biology
M. Chinain, C. M. Gatti, H. T. Darius, J-P Quod, P. A. Tester
Summary: Ciguatera Poisoning (CP) is the most prevalent seafood poisoning caused by ciguatoxins (CTXs) globally, primarily affecting carnivorous fish consumption. The highest incidence rates are reported in the Pacific and Caribbean regions, with an observed expansion of the geographical range of CP in recent years.
Review
Food Science & Technology
Ivannah Pottier, Richard J. Lewis, Jean-Paul Vernoux
Summary: Ciguatera is a widespread poisoning caused by eating marine fish and invertebrates contaminated with ciguatoxins. The various types of ciguatoxins and their properties have been studied extensively, but there is no standardized method for extracting and quantifying ciguatoxins due to limited reference materials. Recent advances in analysis techniques offer new opportunities for detecting and quantifying ciguatoxins, but further research is needed to understand their toxicity and role in ciguatera fish poisoning.
Article
Chemistry, Medicinal
Helene Taiana Darius, Taina Revel, Jerome Viallon, Manoella Sibat, Philippe Cruchet, Sebastien Longo, Donnie Ransom Hardison, William C. Holland, Patricia A. Tester, R. Wayne Litaker, Jennifer R. McCall, Philipp Hess, Mireille Chinain
Summary: This study compared the performance of three methods for quantifying ciguatoxins (CTXs) in Gambierdiscus polynesiensis. The results showed significant correlations between the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS). The CBA-N2a was found to be the most sensitive, while all assays exhibited good repeatability. The combined use of fRBA and/or CBA-N2a for screening and LC-MS/MS for confirmation can efficiently evaluate CTX in Gambierdiscus.
Article
Chemistry, Medicinal
Helene Taiana Darius, Taina Revel, Philippe Cruchet, Jerome Viallon, Clemence Mahana iti Gatti, Manoella Sibat, Philipp Hess, Mireille Chinain
Summary: In 2003, cases of ciguatera poisoning linked to the consumption of deep-water fish occurred in the Gambier Islands, French Polynesia. A study in 2004 found significant levels of ciguatoxins in deep-sea fish samples, leading to the discovery that deep-water fish may act as potential vectors for ciguatera poisoning. This highlights the importance of systematic monitoring of ciguatoxins in all exploited fish species, especially in hotspots like the Gambier Islands.
Article
Plant Sciences
Haifeng Gu, Kenneth Neil Mertens, Amelie Derrien, Gwenael Bilien, Zhen Li, Philipp Hess, Veronique Sechet, Bernd Krock, Ana Amorim, Zhun Li, Vera Pospelova, Kirsty F. Smith, Lincoln MacKenzie, Joo Yeon Yoon, Hyun Jung Kim, Hyeon Ho Shin
Summary: The taxonomy of the genus Gonyaulax is challenging due to the conservative morphology of its thecate structure. However, cysts of Gonyaulax show variations in morphology and are related to fossil-based genera Spiniferites and Impagidinium. Through germination experiments, new species of Gonyaulax were identified and a well-resolved clade based on molecular phylogeny was formed. Additionally, low concentrations of yessotoxin were detected in one G. bohaiensis strain.
JOURNAL OF PHYCOLOGY
(2022)
Article
Chemistry, Medicinal
Helene Taiana Darius, Christelle Paillon, Gerard Mou-Tham, Andre Ung, Philippe Cruchet, Taina Revel, Jerome Viallon, Laurent Vigliola, Dominique Ponton, Mireille Chinain
Summary: The size, weight, age, and growth of fish are not reliable determinants of ciguatoxicity. Larger fish pose an increased risk of poisoning, but even small or low-weight fish can still be toxic.
Article
Chemistry, Medicinal
Helene Taiana Darius, Taina Revel, Jerome Viallon, Manoella Sibat, Philippe Cruchet, Sebastien Longo, Donnie Ransom Hardison, William C. Holland, Patricia A. Tester, R. Wayne Litaker, Jennifer R. McCall, Philipp Hess, Mireille Chinain
Summary: This study compared the performance of three methods for quantifying ciguatoxins (CTXs) in Gambierdiscus polynesiensis. The results showed significant correlations between the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS). The CBA-N2a was found to be the most sensitive, while all assays exhibited good repeatability. The combined use of fRBA and/or CBA-N2a for screening and LC-MS/MS for confirmation can efficiently evaluate CTX in Gambierdiscus.
Article
Environmental Sciences
Laure Vaitiare Andre, Mireille Chinain, Clemence M. Gatti, Vetea Liao, Simon Van Wynsberge, Pauline Tedesco, Serge Andrefouet
Summary: Oyster farming for black pearl production is a crucial industry in French Polynesia, but has been facing crises recently. An option to revive the industry is to reintroduce oysters in strategic benthic locations, promoting recolonization and spat collection. This approach provides managers with a portfolio of restocking options.
MARINE POLLUTION BULLETIN
(2022)
Article
Green & Sustainable Science & Technology
Laure Vaitiare Andre, Simon Van Wynsberge, Mireille Chinain, Clemence Mahana Iti Gatti, Vetea Liao, Serge Andrefouet
Summary: Exploring diversification options can provide tailor-made plans for local managers to adapt to island development questions, reducing dependence on external factors and increasing population resilience to global changes.
Article
Environmental Sciences
Mustapha Ibghi, Mohammed L'bachir El Kbiach, Benlahcen Rijal Leblad, Hicham Aboualaalaa, Fabienne Herve, Manoella Sibat, Adil Chair, Estelle Masseret, Niama Maamour, Mouna Daoudi, Zouher Amzil, Mohamed Laabir
Summary: This study is the first to report the seasonal distribution of benthic harmful algal bloom (BHAB) species in the Strait of Gibraltar. The research found three dominant BHAB dinoflagellates, Ostreopsis spp., Coolia monotis, and Prorocentrum lima. The study also revealed positive correlations between phosphate and temperature with the abundances of these thermophilic BHAB species, while negative correlations were observed with salinity, ammonium, nitrite, nitrate, DIN, nitrogen/phosphate ratio, and suspended material.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2022)
Article
Marine & Freshwater Biology
Jacqui Stuart, Kirsty F. Smith, Lesley Rhodes, J. Sam Murray, Jerome Viallon, Kevin Henry, H. Taiana Darius, Shauna A. Murray, Caroline Dornelles De Azevedo, Phoebe Argyle, Mireille Chinain
Summary: This study aims to further characterize G. honu by assessing its distribution, intraspecific genetic variation, toxicity, and toxin production. The results indicate that G. honu is widely distributed in the Pacific region with no significant intraspecific population structure, and the toxicity diversity is not correlated with geographical patterns.
Article
Microbiology
Olivier Berry, Enora Briand, Alize Bagot, Maud Chaigne, Laurence Meslet-Cladiere, Julien Wang, Olivier Grovel, Jeroen J. Jansen, Nicolas Ruiz, Thibaut Robiou du Pont, Yves Francois Pouchus, Philipp Hess, Samuel Bertrand
Summary: This study explored the chemical interactions between the toxic dinoflagellate Prorocentrum lima and a filamentous fungal species, Aspergillus pseudoglaucus. The results showed a physical contact between the fungal mycelium and dinoflagellate cells, as well as the impact of associated bacteria on the microalgal metabolome response to fungal interaction. The up-regulation of dinoflagellate toxins during co-culture highlights the importance of considering microalgal-fungal interactions in toxin production regulation.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Fisheries
Laure Vaitiare Andre, Simon Van Wynsberge, Mireille Chinain, Serge Andrefouet
Summary: Traditional fishery management schemes have gained recognition worldwide, but the changing environmental and socio-economic contexts require adjustments. This study examines a traditional system on a Polynesian island and suggests a hybrid design combining traditional practices and data-based optimizations.
FISHERIES RESEARCH
(2022)
Article
Chemistry, Analytical
Andrew D. Turner, Karl J. Dean, Monika Dhanji-Rapkova, Sonia Dall'Ara, Florella Pino, Claire McVey, Simon Haughey, Natasha Logan, Christopher Elliott, Ana Gago-Martinez, Jose Manuel Leao, Jorge Giraldez, Ryan Gibbs, Krista Thomas, Ruth Perez-Calderon, Dermot Faulkner, Hugh McEneny, Veronique Savar, Damien Reveillon, Philipp Hess, Fabiola Arevalo, J. Pablo Lamas, Eva Cagide, Mercedes Alvarez, Alvaro Antelo, Mirjam D. Klijnstra, Michalina Oplatowska-Stachowiak, Tim Kleintjens, Nermin Sajic, Michael J. Boundy, Benjamin H. Maskrey, D. Tim Harwood, Jesus M. Gonzalez Jartin, Amparo Alfonso, Luis Botana
Summary: Through interlaboratory assessment, it was found that the current methods of quantifying TTX in shellfish using chemical and immunoassay methods showed good sensitivity, recovery, and repeatability. The results demonstrated that LC-MS/MS methods and ELISA are capable of sensitive, accurate, and reproducible TTX quantitation in shellfish.
JOURNAL OF AOAC INTERNATIONAL
(2023)
Article
Food Science & Technology
Manoella Sibat, Tepoerau Mai, Simon Tanniou, Isabelle Biegala, Philipp Hess, Thierry Jauffrais
Summary: Algal toxins, including emerging toxin groups, pose a potential risk to consumers or shellfish export in New Caledonia. This survey detected several marine microalgal toxin groups for the first time in the area, with higher levels observed during the summer. Although regulated toxins did not exceed limits, pinnatoxin-G was present at the limit recommended by the French Food Safety Authority, indicating a potential risk.
