Article
Chemistry, Medicinal
Zara Ahmed, Lydia C. Powell, Navid Matin, Andrew Mearns-Spragg, Catherine A. Thornton, Ilyas M. Khan, Lewis W. Francis
Summary: The study found that jellyfish collagen exhibited comparable scaffold structural properties and stability to mammalian collagen, and showed similar immunogenic responses in vitro. Jellyfish collagen supported invasion of bovine chondroprogenitor cells in scaffold structures, promoting chondrogenesis in the presence of TGF beta 1, highlighting its potential as a safe and biocompatible biomaterial.
Article
Biotechnology & Applied Microbiology
Paola Nitti, Sanosh Kunjalukkal Padmanabhan, Serena Cortazzi, Eleonora Stanca, Luisa Siculella, Antonio Licciulli, Christian Demitri
Summary: In this study, composite structures made of hydroxyapatite scaffold impregnated with a collagen slurry were designed to mimic bone tissue structure, with the addition of magnesium and silicon ions enhancing mechanical and biological properties. An innovative freeze-drying approach was developed to create open-pore structures for tissue regeneration. The biodegradation behavior of the scaffolds, particularly HA-Mg_Coll scaffolds, showed moderate weight loss and mechanical performance reduction due to collagen dissolution, while also protecting the ceramic structure until degradation, making them suitable candidates for bone remodeling.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Polymer Science
Gustavo A. Rico-Llanos, Sara Borrego-Gonzalez, Miguelangel Moncayo-Donoso, Jose Becerra, Rick Visser
Summary: Collagen type I is widely used in bone tissue engineering due to its excellent biocompatibility and osteoconductivity, but it also has drawbacks such as high biodegradability and low mechanical strength. Recent efforts have been focused on designing and producing collagen implants to enhance bone regeneration.
Article
Pharmacology & Pharmacy
Guan-Xuan Wu, Chun-Yu Chen, Chun-Shien Wu, Lain-Chyr Hwang, Shan-Wei Yang, Shyh-Ming Kuo
Summary: The study examined the effects of farnesol on phenotype restoration in chondrocytes, showing that farnesol promotes collagen and glycosaminoglycan synthesis, leading to the restoration of chondrocyte functions.
Article
Cell Biology
Katarzyna Styczynska-Soczka, Anish K. Amin, Andrew C. Hall
Summary: Abnormal chondrocytes are present in nondegenerate cartilage, suggesting dedifferentiation and production of a weakened matrix. With degeneration, the percentage of normal chondrocytes decreases while abnormal cells and clusters increase, along with collagen type I labelling. The study also found an increase in collagen type I around chondrocytes with degeneration, indicating fibro-cartilaginous repair tissue progression similar to osteoarthritis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Chemistry, Medicinal
Malachy Maher, Veronica Glattauer, Carmine Onofrillo, Serena Duchi, Zhilian Yue, Timothy C. Hughes, John A. M. Ramshaw, Gordon G. Wallace
Summary: This study compares the potential and performance differences of collagen from marine and porcine sources in tissue engineering. Collagen derived from marine sources has lower risk of disease transfer and is more compatible with religious and ethical groups. Although it has inferior mechanical properties and is prone to degradation, marine-derived collagen is still considered a promising biomaterial.
Article
Engineering, Biomedical
Mathaeus Tschaikowsky, Sofia Brander, Vanessa Barth, Ralf Thomann, Bernd Rolauffs, Bizan N. Balzer, Thorsten Hugel
Summary: This study reveals changes in the thickness and composition of collagen fibers at the onset of osteoarthritis. Fibrocartilage-like tissue rich in type I collagen is formed in intact cartilage during the early stages of the disease, while thick fibers are completely absent in advanced osteoarthritis. The findings provide insights into the understanding of other native biological tissues and the development of sustainable biomaterials.
ACTA BIOMATERIALIA
(2022)
Review
Engineering, Biomedical
F. Veronesi, B. Di Matteo, N. D. Vitale, G. Filardo, A. Visani, E. Kon, M. Fini
Summary: Research focused on developing and studying meniscal scaffolds, emphasizing the importance of preserving the meniscus to prevent osteoarthritis. While positive results were seen in preclinical studies, limitations in clinical research point to the need for further innovation.
BIOACTIVE MATERIALS
(2021)
Article
Materials Science, Biomaterials
Tien-Li Ma, Shang-Chih Yang, Ting Cheng, Mei-Yun Chen, Jo-Hsuan Wu, Shu-Lang Liao, Wei-Li Chen, Wei-Fang Su
Summary: Poly(gamma-benzyl-l-glutamate) (PBG) biomimetic scaffolds show potential as candidate materials for corneal nerve regeneration and neurotrophic keratopathy treatment. The PBG scaffolds containing aligned fibers guide the growth and extension of trigeminal ganglia cells, and demonstrate low immune response and neurogenesis induction in in vivo experiments.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Debasish Kumar Ghosh, Prajna Udupa, Akshaykumar Nanaji Shrikondawar, Gandham SriLakshmi Bhavani, Hitesh Shah, Akash Ranjan, Katta M. Girisha
Summary: Aberrant forms of endoplasmic reticulum (ER)-resident chaperones are associated with protein quality control loss in rare diseases. A novel mutation (p.Asp233Asn) in the ER localization signal of MESD was identified in a patient with osteogenesis imperfecta (OI) type XX using whole exome sequencing. The mutant MESDD233N mislocalizes to the cytoplasm, disrupting ER proteostasis and leading to improper folding and aggregation of proteins, including LRP5 and type I collagen. Consequently, there is impaired WNT signaling and cytosolic accumulation of type I collagen aggregates, affecting cell-matrix interactions and cellular stress response.
Article
Engineering, Biomedical
Daniel Martinez-Moreno, Desire Venegas-Bustos, Guillermo Rus, Patricia Galvez-Martin, Gema Jimenez, Juan Antonio Marchal
Summary: This study improves the cell-biomaterial interactions and enhances the chondrogenic properties of highly hydrophobic polyurethane through functionalizing 3D printed scaffolds.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Applied
Yadong Zhao, Kunyu Lu, Xinyue Piao, Yan Song, Libin Wang, Rusen Zhou, Pingping Gao, Heng Yen Khong
Summary: This study evaluated the effect of adding collagen on the gel properties of surimi and observed the influence of collagen type for the first time. Compared to type II, collagen type I has higher water solubility and more charged amino acids, which allows it to better intertwine with surimi myofibrillar proteins, resulting in higher exposure of protein functional domains, more conformational changes of myosin, and stronger formation of chemical forces among proteins. These improvements accelerate the gelation rate, leading to a well-stabilized surimi gel. Surimi gels containing collagen I have more compact structures with uniformly distributed smaller pores compared to those containing collagen II, resulting in higher water holding capacity and better texture. The fortification performance of collagen I in surimi gel and the elucidated interaction mechanism between collagen and myofibrillar protein will guide the further use of collagen as an effective additive in the food industry.
Article
Biochemistry & Molecular Biology
Bastien Bourdon, Frederic Casse, Nicolas Gruchy, Pierre Cambier, Sylvain Leclercq, Sarah Oddoux, Antoine Noel, Jerome E. Lafont, Romain Contentin, Philippe Galera
Summary: Cartilage is a non-innervated and non-vascularized tissue that is prone to chondral defects and osteoarthritis due to its low repair capacity. Collagen hydrolysates derived from fish skin and cartilage show potential as dietary supplements in promoting chondrocyte viability and proliferation, reducing inflammation, and enhancing extracellular matrix synthesis in cartilage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Fan Rui, Kang Jiawei, Hao Yuntao, Liu Xinran, Hu Jiani, Mao Ruixue, Liu Rui, Zhu Na, Xu Meihong, Li Yong
Summary: The study aimed to confirm the preventive and therapeutic effects of undenatured type II collagen (UC II) on osteoarthritis in aging db/db mice and T2DM patients. By UC II intervention, significant improvement in motor functions and serum biomarker levels was observed in aging db/db mice, as well as pain relief, enhanced physical function, and increased walking ability and mobility distance in T2DM patients with knee OA.
Article
Materials Science, Characterization & Testing
Luca Salvatore, Emanuela Calo, Valentina Bonfrate, Deborah Pedone, Nunzia Gallo, Maria Lucia Natali, Alessandro Sannino, Marta Madaghiele
Summary: The optimization of collagen-based scaffolds for tissue engineering involves careful selection of crosslinking methods to achieve the desired properties without negatively impacting cellular responses. Different crosslinking treatments led to varying degrees of chemical and elastic effective crosslink density. EDC treatment promoted intramolecular crosslinks and intermolecular crosslinks, resulting in higher denaturation temperature and lower elastic effective crosslink density compared to aldehyde-based treatments. There was a correlation between denaturation temperature and chemical effective crosslink density, as well as water uptake and elastic effective crosslink density. An exponential relationship was also found between elastic effective crosslink density and in vitro half-life.