Review
Biochemistry & Molecular Biology
Ann K. Hogan, Daniel R. Foltz
Summary: The eukayrotic genome is packaged into chromatin, with nucleosomes being the basic unit composed of DNA and histones. Histones, stable protein species in mammalian cells, play a crucial role in homeostasis. While histones are generally stable, their degradation is necessary in specific conditions for organismal and cellular fitness.
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Adrian Arrieta, Thomas M. Vondriska
Summary: Maintenance of protein folding homeostasis is crucial for cell survival and cell-specific biological processes. Chromatin organization plays a vital role in cell type specification. Nucleosome organization and histone turnover are key factors in chromatin organization. This review focuses on the criticality of nucleosome proteostasis, the involvement of histone chaperones and ATP-dependent chromatin remodelers, and the disrupted nucleosome proteostasis mechanisms in diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Cell Biology
Reuben Franklin, Jernej Murn, Sihem Cheloufi
Summary: The increasing diversity of histone variants and chaperone partners highlights the versatility of nucleosome assembly in different cellular processes, impacting cell identity maintenance and fate decisions. Mutations and expression changes in histones and chaperones are linked to developmental defects and cancer. Modern technologies aid in dissecting the spatiotemporal control of histone variant deposition, providing insights into cell identity maintenance and lineage commitment in health and disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Ruben Rosas, Rhiannon R. Aguilar, Nina Arslanovic, Anna Seck, Duncan J. Smith, Jessica K. Tyler, Mair E. A. Churchill
Summary: The histone chaperone CAF-1 deposits histone H3/H4 dimers onto newly replicated DNA to form the central core of the nucleosome tetrasome. The KER SAH region of CAF-1 plays a crucial role in ensuring the assembly of tetrasomes by linking functional domains within CAF-1 with structural precision.
Article
Multidisciplinary Sciences
Marta Barrientos-Moreno, Douglas Maya-Miles, Marina Murillo-Pineda, Sara Fontalva, Monica Perez-Alegre, Eloisa Andujar, Felix Prado
Summary: Genome duplication occurs through the coordinated action of DNA replication and nucleosome assembly. Defective nucleosome assembly causes DNA lesions and loss of chromatin integrity, which can be restored. The mechanism of chromatin restoration is unknown.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Ann K. Hogan, Kizhakke M. Sathyan, Alexander B. Willis, Sakshi Khurana, Shashank Srivastava, Ewelina Zasadzinska, Alexander S. Lee, Aaron O. Bailey, Matthew N. Gaynes, Jiehuan Huang, Justin Bodner, Celeste D. Rosencrance, Kelvin A. Wong, Marc A. Morgan, Kyle P. Eagen, Ali Shilatifard, Daniel R. Foltz
Summary: Histone chaperones, such as UBR7, play a crucial role in regulating the stability and supply of post-nucleosomal histone complexes. UBR7 interacts with NASP and histones to promote reincorporation of post-nucleosomal H3 complexes, opposing the histone storage functions of NASP. The absence of UBR7 leads to accumulation of NASP-bound post-nucleosomal histones and depletion of H3K4me3-modified histones in chromatin.
Article
Biology
David C. Klein, Santana M. Lardo, Kurtis N. McCannell, Sarah J. Hainer
Summary: The FACT complex maintains cellular pluripotency by regulating nucleosome positioning and transcription activity. FACT interacts with genes such as OCT4, SOX2, and NANOG, binding to both promoter and enhancer elements, and regulates their transcription and expression, thereby influencing cell fate decisions.
Article
Multidisciplinary Sciences
Xiaowei Xu, Shoufu Duan, Xu Hua, Zhiming Li, Richard He, Zhiguo Zhaang
Summary: This study reveals that both parental H3.1 and H3.3 are stably inherited during mitotic cell division in mouse embryonic stem cells, and this process involves histone chaperones Mcm2, Pole3, and Pole4.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Alonso Javier Pardal, Andrew James Bowman
Summary: This study identified importin-5 as the major importin associated with cytoplasmic H3, which transfers its monomeric cargo to nuclear sNASP. Additionally, monomeric H4 interacts specifically with HAT1 and RBBP7. It was found that Imp5 and sNASP compete for binding H3, suggesting a direct interaction site competition requiring GTP-bound Ran for histone transfer.
Article
Biology
Po Jui Chen, Anna B. McMullin, Bryan J. Visser, Qian Mei, Susan M. Rosenberg, David Bates
Summary: In bacteria, sister replisomes are functionally interdependent and remain colocalized throughout replication. Our study reveals the crucial role of physical association between sister replisomes in ensuring an efficient and uninterrupted replication program.
Article
Biochemistry & Molecular Biology
Yoshitami Hashimoto, Kota Sadano, Nene Miyata, Haruka Ito, Hirofumi Tanaka
Summary: DONSON is a novel initiator protein essential for CMG helicase assembly in vertebrates, as demonstrated by experiments in a Xenopus cell-free system. DONSON interacts with GINS and Pol ε to promote replisome assembly, and its chromatin association during replication initiation requires the pre-replicative complex, TopBP1, and kinase activities of S-CDK and DDK.
Article
Biochemistry & Molecular Biology
Simon Obermeyer, Richard Stoeckl, Tobias Schnekenburger, Henna Kapoor, Thomas Stempfl, Uwe Schwartz, Klaus D. Grasser
Summary: In addition to transcriptional initiation, mRNA production is regulated during elongation, and various transcript elongation factors modulate the synthesis of mRNAs by RNA polymerase II (RNAPII) in the chromatin context. TFIIS is an elongation factor that stimulates the transcript cleavage activity of RNAPII, and its deficiency in Arabidopsis plants leads to increased sensitivity to heat stress and decreased expression of heat-induced genes. Further analysis reveals that TFIIS is required for proper RNAPII accumulation at specific nucleosomes and plays a role in reprogramming gene expression for establishing plant thermotolerance.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Hitoshi Kurumizaka, Tomoya Kujirai, Yoshimasa Takizawa
Summary: Chromatin compacts genomic DNA through nucleosome core particles, with versatile structure and dynamics for epigenetic regulation of genomic DNA. Histone variants in higher eukaryotes contribute to nucleosome diversity and play important roles in chromatin epigenetic regulation.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Cell Biology
Tomoya Kujirai, Haruhiko Ehara, Shun-ichi Sekine, Hitoshi Kurumizaka
Summary: In eukaryotes, genomic DNA is packaged into chromatin and the nucleosome acts as a barrier to transcription. The RNA polymerase II elongation complex disassembles the nucleosome during transcription to allow passage. After transcription, the nucleosome is rebuilt by transcription-coupled nucleosome reassembly, which plays a crucial role in preserving epigenetic information. The histone chaperone FACT is involved in nucleosome disassembly, maintenance, and reassembly during transcription in chromatin. Recent structural studies have provided insights into the structural transitions of the nucleosome during transcription.
Review
Biochemistry & Molecular Biology
Celine Duc, Christophe Thiriet
Summary: The review focuses on the remodeling of chromatin during DNA replication in the S-phase, including the eviction of nucleosomes and the assembly of newly synthesized histones. The recycling of parental histones and the nuclear import of histones are also discussed in relation to epigenetic inheritance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
