Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyi Lan, Yan Liang, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Tianxing Gong, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: Skin cancer patients with tumorigenic lesions on their noses often require surgical resection resulting in nasal cartilage removal. Autologous tissue-engineered nasal cartilage grafts can help mitigate the problem, despite the issue of inhomogeneous distribution of cartilage matrix. Advances in 3D bioprinting technology offer customizable and anatomically shaped cartilages without the distribution issues.
Article
Polymer Science
Zahra Gohari, Ahmadali Baghaei, Hossein Mahboudi, Javad Hashemi, Mohammad Rahmati, Maryam Islami, Vahid Mansouri
Summary: This study aims to investigate the potential impact of Ursolic acid (UA) blended with electrospun nanofibrous scaffolds on the chondrogenesis capacity of mesenchymal stem cells (MSCs). The results indicate that the UA blended scaffold significantly improves the chondrogenic differentiation potential of MSCs, suggesting its potential as a candidate for cartilage tissue-engineering applications.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2022)
Article
Chemistry, Applied
Penghui Wang, Yajie Pu, Yanhan Ren, Rong Yang, Wenjie Zhang, Xiaoyan Tan, Wenliang Xue, Shuai Liu, Shuang Li, Bo Chi
Summary: This research introduces a novel hybrid hydrogel system based on sodium alginate and polyglutamic acid, with adjustable gelation time and mechanical strength, prolonged degradation time and reduced swelling rate, dynamic response characteristics, excellent self-healing and injectable ability. The system shows positive cytocompatibility and accelerated regulatory gene expression in cartilage tissue, indicating broad application prospects in future biomedical engineering.
CARBOHYDRATE POLYMERS
(2022)
Article
Pharmacology & Pharmacy
Asma Sepahdar, Abolfazl Nazbar, Saeed Bahadorikhalili, Ghassem Rezaei, Mohammad Ali Shokrgozar, Mohammad Mehdi Dehghan, Hamid Akbari Javar, Shahin Bonakdar
Summary: This paper presents a novel composite scaffold for the regeneration of cartilage tissue. The scaffold is made of a combination of a hydrogel and a controlled release drug delivery system. The release of kartogenin, a chondrogenic-inducing drug, from the scaffold is successfully controlled. The scaffold also demonstrates a porous structure and shows good performance in inducing chondrogenic differentiation of stem cells.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biophysics
Anna M. McDermott, Emily A. Eastburn, Daniel J. Kelly, Joel D. Boerckel
Summary: Mechanical stimulation plays a role in regulating chondrocyte maturation and homeostasis, with the duration of chondrogenic priming affecting the response of engineered cartilage tissue to dynamic compression. Early loading may preserve chondrocyte homeostasis, while delayed loading could support cartilage maturation.
JOURNAL OF BIOMECHANICS
(2021)
Article
Biochemistry & Molecular Biology
Jing Zheng, Yan Xie, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: This study investigated different microenvironmental conditions for the controlled proliferation and chondrogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs). The results showed that collagen sponges promoted cell proliferation and chondrogenic differentiation. Combining collagen sponges with collagen hydrogels and induction factors had the highest promotive effect on chondrogenic differentiation. These findings provide useful information for stem cell chondrogenic differentiation and cartilage tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Panneerselvam Jithendra, Jamal Moideen Muthu Mohamed, Dinesh Annamalai, Rasha Hamed Al-Serwi, Ateya Megahed Ibrahim, Mohamed El-Sherbiny, Abraham Merlin Rajam, Mamdouh Eldesoqui, Nimer Mansour
Summary: The chondrogenic efficacy of aloe vera blended collagen-chitosan (COL-CS-AV) porous scaffold was investigated using articular chondrocytes. The scaffold showed higher chondrogenic efficacy compared to COL-CS, making it a suitable candidate for cartilage tissue engineering. The study utilized various assays and staining techniques to analyze cytocompatibility, GAG content, RNA content, and expression of cartilage-specific genes and collagen type II.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Victoria Barnhouse, Nathan Petrikas, Cody Crosby, Janet Zoldan, Brendan Harley
Summary: The study developed and characterized a three-dimensional perivascular tissue model to investigate the influence of the perivascular secretome on HSC behavior, finding that perivascular conditioned media promoted maintenance of a greater fraction of hematopoietic stem and progenitor cells within a 4-day culture period.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Muthusamy Saranya, Aldeliane M. da Silva, Hanna Karjalainen, Geir Klinkenberg, Ruth Schmid, Birgitte Mcdonagh, Peter P. Molesworth, Margret S. Sigfusdottir, Ane Marit Wagbo, Susana. G. Santos, Cristiana Couto, Ville-Pauli Karjalainen, Shuvashis Das Gupta, Topias Jarvinen, Luisa de Roy, Andreas. M. Seitz, Mikko Finnila, Simo Saarakkala, Anne Marie Haaparanta, Lauriane Janssen, Gabriela S. Lorite
Summary: The demand for engineered scaffolds capable of delivering multiple cues to cells continues to grow. Magnetic-responsive carbon nanotube/collagen/polylactic acid scaffolds are successfully fabricated. In vitro tests using cartilage cells demonstrate that the produced scaffold enhances chondrocytes metabolic activity while maintaining high cell viability and cartilage-relevant extracellular matrix. The exposure to magnetic fields does not affect the cell response.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Cell Biology
Xueqi Wang, Yiming Guan, Shiyu Xiang, Karen L. Clark, Peter G. Alexander, Lauren E. Simonian, Yuhao Deng, Hang Lin
Summary: This review examines the potential of mesenchymal stromal cells (MSCs) in cartilage repair based on animal studies conducted in the past three decades. However, the cartilage tissue derived from MSCs does not possess the same characteristics as native hyaline cartilage, especially in terms of chondrocytic hypertrophy. This undesired phenotype needs to be controlled before MSCs can be effectively used for cartilage repair in clinical settings. Current research focuses on suppressing hypertrophy and modulating the Wnt/β-catenin pathway to inhibit hypertrophy, along with exploring the potential crosstalk between Wnt/β-catenin and other pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Xia Zhao, Daniel A. Hu, Di Wu, Fang He, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Na Ni, Mikhail Pakvasa, Yongtao Zhang, Kai Fu, Kevin H. Qin, Alexander J. Li, Ofir Hagag, Eric J. Wang, Maya Sabharwal, William Wagstaff, Russell R. Reid, Michael J. Lee, Jennifer Moriatis Wolf, Mostafa El Dafrawy, Kelly Hynes, Jason Strelzow, Sherwin H. Ho, Tong-Chuan He, Aravind Athiviraham
Summary: Cartilage, especially articular cartilage, is crucial for joint function and mobility, but has limited self-repair capacity. Cartilage tissue engineering (CTE) holds promise for repair, requiring effective chondrogenic factors, ample chondrogenic progenitors, and cell-friendly, biocompatible scaffold materials for success. Progress in these areas, along with advancements in 3D bio-printing, may improve clinical outcomes of cartilage repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Tanya J. Levingstone, Conor Moran, Henrique Almeida, Daniel J. Kelly, Fergal J. O'Brien
Summary: By utilizing a tri-layered scaffold and co-culturing infrapatellar fat pad MSCs with chondrocytes, the regenerative capacity of cartilage can be enhanced, providing a potential avenue for treating osteochondral defects.
MATERIALS TODAY BIO
(2021)
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Review
Dermatology
T. D. Dobbs, H. Samarendra, S. Hughes, H. A. Hutchings, I. Whitaker
BRITISH JOURNAL OF DERMATOLOGY
(2019)
Editorial Material
Surgery
Thomas D. Dobbs, Jeremy Rodrigues, Andrew M. Hart, Iain S. Whitaker
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2019)
Review
Surgery
Thomas D. Dobbs, John A. G. Gibson, Sarah Hughes, Arron Thind, Benjamin Patel, Hayley A. Hutchings, Iain S. Whitaker
PLASTIC AND RECONSTRUCTIVE SURGERY
(2019)
Article
Engineering, Biomedical
Zita M. Jessop, Ayesha Al-Sabah, Neng Gao, Stuart Kyle, Bethan Thomas, Nafiseh Badiei, Karl Hawkins, Iain S. Whitaker
Article
Chemistry, Applied
Ayesha Al-Sabanh, Stephanie E. A. Burnell, Irina N. Simoes, Zita Jessop, Nafiseh Badiei, Emma Blain, Iain S. Whitaker
CARBOHYDRATE POLYMERS
(2019)
Letter
Surgery
Thomas H. Jovic, Thomas D. Dobbs, Zita M. Jessop, Iain S. Whitaker
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2019)
Review
Physics, Applied
Zita M. Jessop, Susruta Manivannan, Yadan Zhang, Catherine A. Thornton, Roger Narayan, Iain S. Whitaker
APPLIED PHYSICS REVIEWS
(2019)
Article
Orthopedics
Stephen R. Ali, Thomas D. Dobbs, Robert Slade, Iain S. Whitaker
Summary: Through bibliometric and altmetric analyses, this study identified the most influential articles in the field of skin oncology over the past 75 years and found a differential relationship between commonly used metrics and evidence level. The study highlights the importance of considering both bibliometric and altmetric analyses when assessing the impact of research articles as digitalization of research output and consumption increases.
JOURNAL OF PLASTIC SURGERY AND HAND SURGERY
(2021)
Article
Anesthesiology
Thomas D. Dobbs, John A. G. Gibson, Alex J. Fowler, Tom E. Abbott, Tasnin Shahid, Fatemeh Torabi, Rowena Griffiths, Ronan A. Lyons, Rupert M. Pearse, Iain S. Whitaker
Summary: The study shows that in 2020, the volume of surgical activity in England and Wales decreased by 33.6%, with a reduction of 38.6% in elective surgeries and 13.4% in emergency surgeries. The cumulative number of cancelled or postponed procedures was 1,568,664, and it is estimated to increase to 2,358,420 by December 31, 2021.
BRITISH JOURNAL OF ANAESTHESIA
(2021)
Article
Surgery
Stephen R. Ali, Thomas D. Dobbs, Matthew Jovic, Huw Strafford, Beata Fonferko-Shadrach, Arron S. Lacey, Namor Williams, William Owen Pickrell, Hayley A. Hutchings, Iain S. Whitaker
BRITISH JOURNAL OF SURGERY
(2023)
Article
Surgery
A. J. Fowler, T. D. Dobbs, Y. Wan, R. Laloo, S. Hui, D. Nepogodiev, A. Bhangu, I. S. Whitaker, R. M. Pearse, T. E. F. Abbott
Summary: Due to the COVID-19 pandemic, a large number of surgical procedures have been cancelled, leading to millions of patients awaiting surgeries with associated costs in the billions of euros. The safe delivery of surgeries during the pandemic will require substantial additional resources costing millions of euros.
BRITISH JOURNAL OF SURGERY
(2021)
Meeting Abstract
Surgery
Z. M. Jessop, A. Al-Sabah, N. Gao, S. Kyle, B. Thomas, N. Badiei, K. Hawkins, I. S. Whitaker
BRITISH JOURNAL OF SURGERY
(2019)
Article
Medicine, General & Internal
Mussa Mensa, Tom Dobbs, Zita M. Jessop, Iain S. Whitaker
CLINICAL MEDICINE INSIGHTS-CASE REPORTS
(2019)
Article
Surgery
Thomas D. Dobbs, Thomas H. Jovic, Zita M. Jessop, Amanda Kyle, Hayley A. Hutchings, Iain S. Whitaker
PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN
(2018)