Article
Biochemistry & Molecular Biology
Jishizhan Chen, Jia Hu, Wenhui Song
Summary: This study identified three hub genes (IL6, MMP2, and EP300) highly associated with distraction-induced osteogenesis of MSCs, providing a new understanding of optimizing DO through targeted gene therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Feng Wang, Wenbo Wang, Lingchi Kong, Li Shi, Mengwei Wang, Yimin Chai, Jia Xu, Qinglin Kang
Summary: This study found that ADM2 accelerates bone regeneration by enhancing the osteogenic differentiation and pro-angiogenic potential of BMSCs, possibly through the NF-κB/β-catenin and AKT/β-catenin pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Laidi Wu, Zhixin Liu, Li Xiao, Mi Ai, Yingguang Cao, Jing Mao, Ke Song
Summary: This review comprehensively elucidates the specific roles of Gli1+ MSCs in craniofacial bone osteogenesis and tissue repair, offering new insights into bone regeneration therapy.
Article
Cell & Tissue Engineering
Junjie Shen, Yi Sun, Xuanzhe Liu, Yu Zhu, Bingbo Bao, Tao Gao, Yimin Chai, Jia Xu, Xianyou Zheng
Summary: EGFL6 was found to stimulate angiogenesis and osteogenic differentiation through the Wnt/beta-catenin signaling pathway in vitro, and promoted CD31(hi)EMCN(hi) type H-positive capillary formation in a rat tibia distraction osteogenesis model. It enhanced the osteogenic differentiation potential of BMSCs and accelerated bone regeneration by stimulating angiogenesis.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Feng Wang, Huijuan Qian, Lingchi Kong, Wenbo Wang, Xiaoyu Wang, Ze Xu, Yimin Chai, Jia Xu, Qinglin Kang
Summary: Astragaloside IV (AS-IV) accelerates bone regeneration during distraction osteogenesis by enhancing osteogenesis and preosteoclast-induced angiogenesis simultaneously, partially through AKT/GSK-3 beta/beta-catenin signaling. These findings suggest that AS-IV may serve as a potential bioactive molecule for promoting the coupling of osteogenesis and angiogenesis, and imply that AKT/GSK-3 beta/beta-catenin signaling may be a promising therapeutic target for patients during distraction osteogenesis treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cell & Tissue Engineering
Qi Chang, Masahito Fujio, Makoto Tsuboi, Huiting Bian, Masashi Wakasugi, Hideharu Hibi
Summary: This study evaluated high-mobility group box 1 (HMGB1) as a novel therapeutic target for distraction osteogenesis (DO) treatment. The results showed that HMGB1 promotes bone regeneration by recruiting PDGFRa and Sca-1 positive cells and endothelial progenitor cells, as well as accelerating angiogenesis. Therefore, HMGB1 may be a new therapeutic target for the acceleration of DO-driven bone regeneration.
Review
Cell Biology
Kangkang Zha, Yue Tian, Adriana C. Panayi, Bobin Mi, Guohui Liu
Summary: Bone tissue engineering offers a new approach to treating bone defects, with mesenchymal stem cells being a promising seed cell due to their functions in osteogenic differentiation. Improving methods of origin selection, culture conditions, and biophysical stimulation can enhance MSC osteogenesis and contribute to the development of MSC-based bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Engineering, Biomedical
Ziyi Feng, Meiqi Jin, Junzhi Liang, Junning Kang, Huazhe Yang, Shu Guo, Xiaoting Sun
Summary: Bone damage may require bone grafting and bone tissue engineering can be an alternative strategy. Mesenchymal stem cells (MSCs) are important for tissue engineering due to their ability to differentiate into various cell types. The regulation of MSCs differentiation and the role of mitochondria in this process are crucial for efficient bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Nanoscience & Nanotechnology
Haoyu Zhao, Yachao Jia, Feng Wang, Yimin Chai, Chunfu Zhang, Jia Xu, Qinglin Kang
Summary: In this study, mesoporous silica-coated magnetic nanoparticles doped with cobalt ions were synthesized to accelerate bone repair. The results showed that local injection of these nanoparticles significantly accelerated bone healing in a mouse model. This study’s findings are important for expediting the treatment process of bone fractures.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Physiology
Yanshi Liu, Jialin Liu, Feiyu Cai, Kai Liu, Xiaoxu Zhang, Aihemaitijiang Yusufu
Summary: Hypoxia therapy during the consolidation phase of distraction osteogenesis enhances bone regeneration by accelerating bone formation and remodeling.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell & Tissue Engineering
Feng Wang, Lingchi Kong, Wenbo Wang, Li Shi, Mengwei Wang, Yimin Chai, Jia Xu, Qinglin Kang
Summary: The study demonstrates that ADM2 enhances diabetic bone regeneration by attenuating imbalances in macrophage polarization induced by AGEs, partly through the PPARγ/NF-κB signaling pathway, and by improving AGE-impaired osteogenic differentiation of BMSCs simultaneously.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Ting-Wei Kao, Arthur Chiou, Keng-Hui Lin, Yi-Shiuan Liu, Oscar Kuang-Sheng Lee
Summary: Research shows that mechanical stimulation affects the intracellular viscoelasticity of human mesenchymal stem cells, with cells cultured in rigid matrix displaying higher elastic and viscous moduli. This effect remains consistent throughout the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Jingwen Zhuang, Ruiyue Hang, Ruoyue Sun, Yanshu Ding, Xiaohong Yao, Ruiqiang Hang, Hui Sun, Long Bai
Summary: Exosomes secreted by bone marrow mesenchymal stem cells have multifaceted effects on osseointegration and hold great promise as a safe, efficient, and ethically unrestricted therapy.
FRONTIERS IN CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Xuanzhe Liu, Yi Sun, Junjie Shen, Hong Sung Min, Jia Xu, Yimin Chai
Summary: Distraction osteogenesis (DO) is a powerful method for reconstructing bone defects in extremities, but it has a lengthy consolidation period. This study investigates the use of strontium-doped mesoporous silica nanoparticles as a dual-ion releasing drug delivery system to accelerate mineralization during DO. The system promotes osteogenesis and angiogenesis, while inhibiting bone resorption.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Review
Cell Biology
Xinyun Xu, Ling Zhao, Paul D. Terry, Jiangang Chen
Summary: Mesenchymal stem cells from bone marrow can differentiate into either adipocytes or osteoblasts, and external stimuli such as environmental contaminants, heavy metals, dietary, and physical factors can influence this fate decision. Maintaining a balance between osteogenesis and adipogenesis is crucial for bone homeostasis, and disruptions in lineage commitment of these stem cells are associated with various bone disorders. This review focuses on understanding how external stimuli affect the fate of mesenchymal stem cells and calls for future studies to investigate the impact on bone health and underlying mechanisms of differentiation.
Article
Clinical Neurology
Yujia Wang, Mengheng Li, Chi-On Chan, Guangpu Yang, Jacky Chun-Kit Lam, Brian Chun-Sum Law, Tsz-ping Lam, Alec Lik-Hang Hung, Jack Chun-Yiu Cheng, Daniel Kam-Wah Mok, Wayne Yuk-Wai Lee
Summary: Abnormal energy metabolism, particularly dysregulated galactose metabolism and glycolysis, is associated with the progression of adolescent idiopathic scoliosis (AIS) and may be modulated by the LBX1 gene.
Article
Biotechnology & Applied Microbiology
Bin Wang, Mengru Pang, Yancheng Song, Haixing Wang, Pan Qi, Shanshan Bai, Xiaoxuan Lei, Shikun Wei, Zhixian Zong, Sien Lin, Xiaoting Zhang, Xiaotong Cen, Xia Wang, Yongkang Yang, Yuan Li, Yan Wang, Hongjie Xu, Lin Huang, Micky Tortorella, Biao Cheng, Yukwai Lee, Dajiang Qin, Gang Li
Summary: This study explores the potential role of human fetal mesenchymal stem cell secretome (HFS) in wound healing and addresses obstacles in clinical application. Results show that encapsulated and protected secretome significantly enhances wound healing by promoting vascularization and inhibiting inflammation. Bioinformatics analysis identifies exosome production-related signaling pathways and the heat-shock protein family as potential biomarkers and quality control for secretome production.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Qiangqiang Li, Rongliang Wang, Zhe Zhang, Haixing Wang, Xiaomin Lu, Jiajun Zhang, Alice Pik-Shan Kong, Xiao Yu Tian, Hon-Fai Chan, Arthur Chi-Kong Chung, Jack Chun-Yiu Cheng, Qing Jiang, Wayne Yuk-Wai Lee
Summary: Exercise in later life is important for bone health, but the effectiveness may be impaired due to the degeneration of osteocyte activity and lacunocanalicular networks. In this study, it was found that the deletion of Sirt3 in osteocytes could impair the formation of dendritic processes and inhibit bone gain in response to exercise. Mechanistic studies revealed that Sirt3 regulates E11/gp38 through the PKA/CREB signaling pathway. Additionally, the Sirt3 activator honokiol enhanced osteocyte sensitivity and reduced bone loss in aged mice.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Medicine, Research & Experimental
Zhonghua Peng, He Pang, Hang Wu, Xin Peng, Qichao Tan, Sien Lin, Bo Wei
Summary: Severe bone trauma can lead to poor bone healing and nonunion. This study investigated the mechanism by which CCL2 promotes angiogenesis in bone defects. The results showed that CCL2 promoted proliferation, migration, and tube formation in HUVECs and upregulated the expression of various proteins involved in signaling pathways related to angiogenesis. The study also demonstrated that CCL2 increased the expression of VEGF in the newly formed bone area, suggesting its potential as a therapeutic approach for bone defect repair and reconstruction through angiogenesis-osteogenesis coupling.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Engineering, Biomedical
Alexander Stahl, Dake Hao, Janos Barrera, Dominic Henn, Sien Lin, Seyedsina Moeinzadeh, Sungwoo Kim, William Maloney, Geoffrey Gurtner, Aijun Wang, Yunzhi Peter Yang
Summary: Conventional synthetic vascular grafts have high failure rates due to mismatched mechanical properties with native vessels and poor regenerative potential. In this study, we developed a new bioactive synthetic vascular graft with tailored pore sizes to promote pro-regenerative macrophage populations and improve vascular regeneration. The grafts showed similar mechanical properties to native blood vessels and maintained patency and vascular regeneration in animal models.
BIOACTIVE MATERIALS
(2023)
Review
Endocrinology & Metabolism
Junying Hua, Jianping Huang, Gang Li, Sien Lin, Liao Cui
Summary: Long-term or supra-physiological dose of glucocorticoid application can lead to impaired bone growth and osteoporosis, especially in children. Despite the availability of drugs for treating osteoporosis, there is currently no specific drug to mitigate these severe side effects.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Medicine, General & Internal
Zhichong Wu, Xiufen Zhu, Leilei Xu, Zhen Liu, Zhenhua Feng, Vivian Wing Yin Hung, Jack Chun Yiu Cheng, Yong Qiu, Wayne Y. W. Lee, Tsz Ping Lam, Zezhang Zhu
Summary: The prevalence of low bone-mineral density (BMD) is higher in boys with severe adolescent idiopathic scoliosis (AIS) than in girls. Factors such as sex, body mass index, serum alkaline phosphatase, and potassium are independent risk factors of low BMD in severe AIS patients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Engineering, Biomedical
Yucong Li, Linlong Li, Ye Li, Lu Feng, Bin Wang, Ming Wang, Haixing Wang, Meiling Zhu, Yongkang Yang, Erik I. Waldorff, Nianli Zhang, Ingmar Viohl, Sien Lin, Liming Bian, Wayne Yuk-Wai Lee, Gang Li
Summary: In this study, a new tissue engineering strategy for cartilage defect treatment was proposed using an injectable self-healing adhesive hydrogel and pulsed electromagnetic field (PEMF) treatment. The results demonstrated the superior clinical potential of this strategy in promoting chondrogenesis and cartilage repair.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Xian Xie, Zhuo Li, Xuefeng Yang, Boguang Yang, Zhixian Zong, Xuemei Wang, Liting Duan, Sien Lin, Gang Li, Liming Bian
Summary: In this study, a peptide fiber-reinforced hydrogel was developed, where the stiff β-sheet peptide fiber acted as a multivalent cross-linker to enhance the macroscopic stability of the hydrogel. The dynamic imine cross-link between the peptide fiber and polymer network endowed the hydrogel with a microscopically dynamic network. The resulting fibrillar nanocomposite hydrogel, with its cell-adaptable dynamic network, significantly enhanced cell-matrix and cell-cell interactions, promoting mechanotransduction, metabolic energetics, and osteogenesis of encapsulated stem cells. Additionally, the hydrogel could deliver a fiber-attached inductive drug to further enhance bone regeneration. This work provides valuable guidance for the design of cell-adaptive and bioactive biomaterials for therapeutic applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Sien Lin, Hirotsugu Maekawa, Seyedsina Moeinzadeh, Elaine Lui, Hossein Vahid Alizadeh, Jiannan Li, Sungwoo Kim, Michael Poland, Benjamin C. Gadomski, Jeremiah T. Easley, Jeffrey Young, Michael Gardner, David Mohler, William J. Maloney, Yunzhi Peter Yang
Summary: In this study, the authors propose the use of an osteoinductive and biodegradable intramedullary implant to promote early bony bridging and prevent complications in the treatment of large bone defects. The implant releases bone morphogenetic protein-2 (BMP-2) and accelerates bone formation and remodeling, leading to successful bone fusion without pin tract infection or docking site nonunion.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Haixing Wang, Sien Lin, Lu Feng, Baozhen Huang, Xuan Lu, Zhengmeng Yang, Zhaowei Jiang, Yu-Cong Li, Xiaoting Zhang, Ming Wang, Bin Wang, Lingchi Kong, Qi Pan, Shanshan Bai, Yuan Li, Yongkang Yang, Wayne Yuk Wai Lee, Peter D. Currie, Changshuang Lin, Yanfu Jiang, Juyu Chen, Micky D. Tortorella, Hongyi Li, Gang Li
Summary: Recent studies have shown that T cells play a role in maintaining bone homeostasis, suggesting them as potential therapeutic targets for bone-related conditions. This study reports that low-dose administration of SEC2 2M-118 can alleviate bone loss in mice by modulating T cells. The treatment increases trabecular bone mass by promoting bone formation and this effect is diminished in T-cell-deficient mice. The key factor mediating these effects is IFN-gamma, which stimulates signaling pathways and enhances osteogenic differentiation.
Review
Medicine, General & Internal
Shuai Liu, Jiang-Ming Yu, Yan-Chang Gan, Xiao-Zhong Qiu, Zhe-Chen Gao, Huan Wang, Shi-Xuan Chen, Yuan Xiong, Guo-Hui Liu, Si-En Lin, Alec McCarthy, Johnson V. John, Dai-Xu Wei, Hong-Hao Hou
Summary: Biomimetic materials based on natural biomaterials offer cells biochemical and biophysical cues that mimic the in vivo extracellular matrix, making them ideal for tissue engineering and regenerative medicine. This paper provides an overview of recent progress in biomimetic natural biomaterials (BNBMs), including their preparation, functionality, potential applications, and future challenges. The paper also highlights recent advances in the fabrication and functionalization of BNBMs, as well as their applications in tissue engineering.
MILITARY MEDICAL RESEARCH
(2023)
Review
Cell & Tissue Engineering
Ranyang Tao, Bobin Mi, Yiqiang Hu, Sien Lin, Yuan Xiong, Xuan Lu, Adriana C. C. Panayi, Gang Li, Guohui Liu
Summary: This review outlines the role of the peripheral nervous system (PNS) in bone regeneration, including the conserved role of nerves in tissue regeneration across different species and the distribution of the PNS in the skeletal system. It summarizes the communication between the PNS and bone-lineage cells, vasculature, and immune cells in the bone microenvironment. The review concludes that the PNS regulates bone regeneration through neuropeptides or neurotransmitters and cells in the peripheral nerves, and understanding this role can inform the development of strategies for bone repair and regeneration based on bone-nerve crosstalk.