Article
Biochemistry & Molecular Biology
Zhonghua Zeng, Yuanhao Zhou, Yibin Xu, Song Wang, Baikui Wang, Zihan Zeng, Qi Wang, Xiaolin Ye, Lu Jin, Min Yue, Li Tang, Peng Zou, Pengwei Zhao, Weifen Li
Summary: This study investigated the effects of Bacillus amyloliquefaciens SC06 on obesity, intestinal microbiota, and bile acid metabolism in ob/ob mice. The results showed that SC06 could ameliorate obesity in male ob/ob mice by reshaping the intestinal microbial composition, changing metabolites, and regulating bile acid metabolism via the FXR signaling pathway.
Article
Endocrinology & Metabolism
Pernille Baumann Toft, Amanda Marie Vanslette, Kajetan Trost, Thomas Moritz, Matthew Paul Gillum, Fredrik Baeckhed, Tulika Arora
Summary: p-cresol is a microbial metabolite that suppresses transcript levels of gut hormones and regulates small intestinal transit in mice. It inhibits Gcg expression and reduces glucagon-like peptide-1 (GLP-1) secretion in vitro. In mice, p-cresol administration reduces transcript levels of Gcg and other gut hormones in the colon, but does not affect plasma GLP-1 levels or glucose tolerance.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eun Bee Choi, Jae Hun Jeong, Hye Min Jang, Yu Jeong Ahn, Kyu Hyeon Kim, Hyeong Seok An, Jong Youl Lee, Eun Ae Jeong, Jaewoong Lee, Hyun Joo Shin, Kyung Eun Kim, Gu Seob Roh
Summary: The study revealed that elevated levels of LCN2 in skeletal muscle of obese individuals are associated with inflammation and oxidative stress related to muscle atrophy.
Article
Microbiology
Franc Suriano, Sara Vieira-Silva, Gwen Falony, Martin Roumain, Adrien Paquot, Rudy Pelicaen, Marion Regnier, Nathalie M. Delzenne, Jeroen Raes, Giulio G. Muccioli, Matthias Van Hul, Patrice D. Cani
Summary: Results from studying ob/ob and db/db mice show significant differences in glucose metabolism, fat distribution, liver steatosis, and inflammatory profiles, despite both being severely obese. These differences may be attributed to variations in gut microbiota composition, short-chain fatty acids, bile acid metabolism, inflammatory tones, serum LPS concentration, and leptin signaling alterations.
Article
Pharmacology & Pharmacy
Lucas Opazo-Rios, Antonio Tejera-Munoz, Manuel Soto Catalan, Vanessa Marchant, Carolina Lavoz, Sebastian Mas Fontao, Juan Antonio Moreno, Marta Fierro Fernandez, Ricardo Ramos, Beatriz Suarez-Alvarez, Carlos Lopez-Larrea, Marta Ruiz-Ortega, Jesus Egido, Raul R. Rodrigues-Diez
Summary: The study analyzed the differential expression of miRNA in the kidneys of BTBR ob/ob mice using Next-Generation Sequencing and found 99 miRNAs significantly increased in diabetic kidneys, with none being significantly decreased. These miRNAs may be associated with inflammatory and immune processes, highlighting the importance of inflammation in the pathogenesis of diabetic nephropathy.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carlos M. Cuesta, Maria Pascual, Raul Perez-Moraga, Irene Rodriguez-Navarro, Francisco Garcia-Garcia, Juan R. Urena-Peralta, Consuelo Guerri
Summary: Chronic alcohol abuse leads to inflammatory response and dysbiosis in the gut microbiome. TLR4 plays a key role in determining the gut microbiota, which is involved in the inflammatory response induced by alcohol consumption.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Integrative & Complementary Medicine
Yarong Liu, Hongfei Wu, Tian Wang, Xiaoyan Shi, Hai He, Hanwen Huang, Yulong Yang, Min Dai
Summary: Dysbiosis of gut microbiota is a contributing factor to the development of atherosclerosis, and paeonol can alleviate atherosclerosis by regulating gut microbiota composition and metabolites. We identified alpha-hydroxyisobutyric acid as a harmful microbial metabolite reduced by paeonol. Furthermore, we found that alpha-hydroxyisobutyric acid induces endothelial inflammation via the ROS/TXNIP/NLRP3 pathway.
CHINESE JOURNAL OF NATURAL MEDICINES
(2023)
Article
Biochemistry & Molecular Biology
Lucas Opazo-Rios, Manuel Soto-Catalan, Iolanda Lazaro, Aleix Sala-Vila, Luna Jimenez-Castilla, Macarena Orejudo, Juan Antonio Moreno, Jesus Egido, Sebastian Mas-Fontao
Summary: This study characterized the pathophysiological mechanism involved in the development of MAFLD using the BTBR ob/ob mouse model. The results showed that BTBR ob/ob mice exhibited MAFLD features similar to humans, with overactivation of inflammatory response, oxidative stress, and de novo lipogenesis signaling pathways. This research is important for understanding the transition from steatosis to steatohepatitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Namrata Iyer, Sinead C. Corr
Summary: Inflammatory bowel disease (IBD) is a chronic inflammatory disease with a multifactorial etiology involving genetic, microbial, and environmental factors. The intestinal epithelium plays a central role in disease pathogenesis, while microbial metabolites are key regulators of gut homeostasis and the pathogenesis of IBD.
Article
Chemistry, Medicinal
Aneta Grycova, Hansol Joo, Vitezslav Maier, Peter Illes, Barbora Vyhlidalova, Karolina Poulikova, Lucia Sladekova, Petr Nadvornik, Radim Vrzal, Lenka Zemankova, Petra Pecinkova, Martin Poruba, Iveta Zapletalova, Rostislav Vecera, Pavel Anzenbacher, Jiri Ehrmann, Peter Ondra, Jong-Wha Jung, Sridhar Mani, Zdenek Dvorak
Summary: By synthesizing a series of ITE chemical mimics, we identified ITE-CONHCH3 as a highly potent AhR agonist with high affinity and metabolic stability. ITE-CONHCH3 can induce nuclear translocation and dimerization of AhR, enhance AhR binding, and induce AhR-regulated gene expression in an AhR-dependent manner. Additionally, ITE-CONHCH3 exhibited protective effects in a mouse colitis model.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Nutrition & Dietetics
Sashuang Dong, Chengwei Wu, Wencan He, Ruimin Zhong, Jing Deng, Ye Tao, Furong Zha, Zhenlin Liao, Xiang Fang, Hong Wei
Summary: This study analyzed the changes in gut microbes, gut bacteriophages, and metabolites in ob/ob and ApoE(-/-) mice, which are widely used as models for studying obesity and atherosclerosis. The results revealed significant differences in the abundance of certain microbial species and metabolites between the disease models and wild-type mice, providing insights into the possible causes of these metabolic diseases and laying a foundation for further research on the regulation of food nutrients in these models.
FRONTIERS IN NUTRITION
(2022)
Article
Nutrition & Dietetics
Kaixia Niu, Pengpeng Bai, Junyang Zhang, Xinchi Feng, Feng Qiu
Summary: Cytidine and uridine are important metabolites involved in the pyrimidine metabolism pathway. While uridine has been known for its ability to regulate lipid metabolism, the potential effect of cytidine on lipid metabolism disorder has not been explored. To investigate this, ob/ob mice were treated with cytidine for five weeks, and the impact on lipid metabolism disorder was assessed through various tests. The results showed that cytidine could ameliorate dyslipidemia and improve hepatic steatosis in ob/ob mice by modulating the composition of gut microbiota, particularly by increasing the abundance of short-chain fatty acids-producing bacteria. This suggests that cytidine supplementation could be a potential therapeutic approach for dyslipidemia.
Article
Biochemistry & Molecular Biology
Kaixia Niu, Pengpeng Bai, Bingbing Yang, Xinchi Feng, Feng Qiu
Summary: This study found that asiatic acid (AA) can improve glucolipid metabolism disorders by regulating pyrimidine metabolism, activating PPAR-gamma, and modulating gut microbiota, based on experiments conducted on ob/ob mice.
Article
Chemistry, Multidisciplinary
Kun Chen, Guoyong Dai, Shengqiu Liu, Yongge Wei
Summary: In this study, it was found that the administration of POVs can significantly decrease body weight, reduce adipose tissue accumulation, and alleviate adipose tissue inflammation in mice. Additionally, POVs can protect the anti-obesogenic population of iNKT cells, thus alleviating visceral adipose tissue inflammation induced by high-fat diet. These findings suggest that supplementation of POVs may be an effective way to combat obesity and metabolic disorders while reducing metabolic inflammation.
CHINESE CHEMICAL LETTERS
(2023)
Article
Oncology
Giorgia Renga, Emilia Nunzi, Marilena Pariano, Matteo Puccetti, Marina Maria Bellet, Giuseppe Pieraccini, Fiorella D'Onofrio, Ilaria Santarelli, Claudia Stincardini, Franco Aversa, Francesca Riuzzi, Cinzia Antognelli, Marco Gargaro, Oxana Bereshchenko, Maurizio Ricci, Stefano Giovagnoli, Luigina Romani, Claudio Costantini
Summary: Microbial metabolites at the interface between microbes and the host immune system can optimize immune checkpoint inhibitor (ICI) therapy without impairing its antitumor activity. This study provides a proof-of-concept demonstration for the development of new therapeutic strategies.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Chemistry, Medicinal
Takuma Kobayashi, Takeshi Toyoda, Yuya Tajima, Shinji Kishimoto, Yuta Tsunematsu, Michio Sato, Kohei Matsushita, Takanori Yamada, Yuko Shimamura, Shuichi Masuda, Masako Ochiai, Kumiko Ogawa, Kenji Watanabe, Takeji Takamura-Enya, Yukari Totsuka, Keiji Wakabayashi, Noriyuki Miyoshi
Summary: The study suggests that a novel metabolite MxMxBD may increase the risk of bladder carcinogenesis by exhibiting stronger mutagenicity and genotoxicity compared to o-Ans. Additionally, heterodimerizations were observed when o-Ans was coincubated with o-Tol or aniline, highlighting the importance of considering both homodimerizations and heterodimerizations in evaluating the combined exposure risk of bladder carcinogenesis.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Chemistry, Applied
Yoji Kato, Yui Kishi, Yayako Okano, Masaki Kawai, Michiyo Shimizu, Naoko Suga, Chisato Yakemoto, Mai Kato, Akika Nagata, Noriyuki Miyoshi
Summary: Reports on the thermal stability of manuka honey in terms of food processing have been limited. This study found that heating resulted in significant decreases in MGO and MAP in manuka honey. The mechanism for the decrease in MGO involves an amino-carbonyl reaction, while the decrease in MAP is influenced by the volume of headspace in a vessel. Additionally, heating can cause signature molecules in honey to vaporize.
Article
Biochemistry & Molecular Biology
Hiroki Matsuyama, Wataru Tanaka, Noriyuki Miyoshi, Tatsuo Miyazaki, Hideyuki Michimoto, Hiroyuki Sakakibara
Summary: The study evaluated the effects of daily consumption of raw or sun-dried radishes on apolipoprotein E-deficient mice. Consumption of both diets led to decreased body weight gain, visceral fat weight, and serum triglyceride levels in the mice. Additionally, oral fat tolerance tests showed that pretreatment with the radish diets mitigated the increase in serum triglyceride levels after oil administration.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Review
Food Science & Technology
Tomokazu Ohishi, Sumio Hayakawa, Noriyuki Miyoshi
Summary: Epidemiological studies have shown that consumption of green tea, coffee, wine, and curry may reduce the risk of various cancers, although the effects can differ depending on the specific cancer site. Compounds found in these foods, such as chlorogenic acid, curcumin, epigallocatechin gallate, and resveratrol, can act as antioxidants or prooxidants to modulate reactive oxygen species (ROS) and miRNA expression, thereby exerting anticancer effects.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Geriatrics & Gerontology
Takuji Suzuki, Kai Aoki, Kotomi Shimokobe, Shoko Omiya, Chiharu Funayama, Takumi Takahashi, Minori Kato
Summary: The ability of the small intestine to perform various functions declines with age and the mechanism that causes intestinal senescence remains unclear. This study evaluated age-related changes in the jejunum and ileum of mice and found that disorders affecting the differentiation/maturation system of intestinal epithelial cells contribute to spontaneous senescence of the small intestine. Brain dysfunction was also observed to enhance intestinal senescence. The brain-intestinal nervous system may play a role in maintaining villous morphology and nutrients uptake.
EXPERIMENTAL GERONTOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yasukiyo Yoshioka, Tomokazu Ohishi, Yoriyuki Nakamura, Ryuuta Fukutomi, Noriyuki Miyoshi
Summary: Consumption of coffee, tea, wine, curry, and soybeans has been linked to a lower risk of cancer. Polyphenols play a major role in the anticancer effects of these foods, influencing ROS-mediated pathways and miR expression. Further research can provide more convincing evidence.
Article
Nutrition & Dietetics
Takuji Suzuki, Yuki Sato, Sumire Kadoya, Takumi Takahashi, Moeko Otomo, Hanna Kobayashi, Kai Aoki, Mai Kantake, Maika Sugiyama, Ronaldo P. Ferraris
Summary: Research has shown that allulose has a greater impact on the intestinal transcriptome compared to fructose and glucose, potentially stimulating nutrient transport, metabolism, and digestive system development. Additionally, allulose can restore small intestinal structure and function in rats and may act through the secretion of GLP-2.
Article
Chemistry, Medicinal
Takuma Kobayashi, Shinji Kishimoto, Shogo Watanabe, Yasukiyo Yoshioka, Takeshi Toyoda, Kumiko Ogawa, Kenji Watanabe, Yukari Totsuka, Keiji Wakabayashi, Noriyuki Miyoshi
Summary: This study identifies the formation of several p-semidine-type dimers, including MMBD and MxMxBD, during the metabolic conversion of o-Tol and o-Ans. The dimers containing the o-Ans moiety showed faster dimerization rates but decreased levels with prolonged incubation. These findings suggest the potential significance of the homo-and hetero-dimers of monocyclic aromatic amines in bladder carcinogenesis and the combined exposure risk.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Hiroki Tanabe, Takuji Suzuki, Tomokazu Ohishi, Mamoru Isemura, Yoriyuki Nakamura, Keiko Unno
Summary: Epidemiological studies have shown that consumption of green tea has beneficial effects against cancer. Basic studies have provided evidence that epigallocatechin gallate (EGCG) is a major contributor to these effects. In this review, the effects of EGCG on various types of matrix metalloproteinases (MMPs) and the underlying mechanisms are discussed, highlighting potential strategies for developing anti-MMP agents with beneficial effects on cancer and other diseases.
Review
Biochemistry & Molecular Biology
Tomokazu Ohishi, Noriyuki Miyoshi, Mari Mori, Miki Sagara, Yukio Yamori
Summary: Plant polyphenols, such as genistein and epigallocatechin-3-gallate, have been studied for their potential health benefits, particularly in relation to cancer and cardiovascular diseases. However, there are conflicting findings regarding the effects of these flavonoids, with some studies showing positive effects while others do not. Factors such as recall bias and the measurement of metabolites in urine samples can contribute to these inconsistencies. Further clinical studies considering different confounding factors are needed to provide evidence for the actual impact of dietary flavonoids on human diseases. One possible mechanism involves the regulation of reactive oxygen species and 5'-adenosine monophosphate-activated protein kinase by flavonoids.
Review
Biochemistry & Molecular Biology
Sumio Hayakawa, Tomokazu Ohishi, Yumiko Oishi, Mamoru Isemura, Noriyuki Miyoshi
Summary: Polyphenols have anticancer effects by acting as antioxidants and modulating cell signaling pathways, with ncRNA modulation enhancing these effects.
Article
Nutrition & Dietetics
Kazuaki Iguchi, Koji Nagashima, Jun Mochizuki, Hiroyuki Yamamoto, Keiko Unno, Noriyuki Miyoshi
Summary: Several studies have shown that the consumption of certain mushroom species can affect testosterone production in animals. Enokitake mushrooms and vegetables containing adenosine might influence testosterone production. This study investigated the effects of enokitake and adenosine on testosterone production in testicular cells in mice models. The results suggest that intake of adenosine-containing mushrooms and vegetables, particularly enokitake, may be beneficial for increasing testicular testosterone production and combating aging and fatigue.
Article
Biochemistry & Molecular Biology
Yasukiyo Yoshioka, Shiori Oishi, Keita Onoda, Katsumi Shibata, Noriyuki Miyoshi
Summary: The study found that diosgenin is an effective functional food for preventing glucocorticoid-induced skeletal muscle atrophy. It acts by inhibiting protein expression, improving phosphorylation levels, and blocking nuclear translocation.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Review
Biochemistry & Molecular Biology
Takuji Suzuki, Tomokazu Ohishi, Hiroki Tanabe, Noriyuki Miyoshi, Yoriyuki Nakamura
Summary: Matrix metalloproteinases (MMPs) are zinc-dependent metalloproteinases that are involved in various diseases. Polyphenols have been demonstrated to have beneficial effects on these diseases by suppressing MMP gene expression and enzyme activity, as well as inhibiting reactive oxygen species and directly binding to MMPs. Molecular docking analyses can provide a structural basis for the development of polyphenol-based drugs with anti-inflammatory properties.
Article
Nutrition & Dietetics
Takuji Suzuki, Kai Aoki, Takumi Takahashi, Natsumi Masuda, Saya Yamauchi, Kunihiro Kishida
Summary: Honey oral intake is effective in recovering and maintaining small intestinal functions and can potentially be used as a supplement for pro-small intestinal function recovery in the total parenteral nutrition (TPN) rat model.
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
(2022)