Article
Genetics & Heredity
Daiki Shikata, Shogo Matoba, Masashi Hada, Akihiko Sakashita, Kimiko Inoue, Atsuo Ogura
Summary: In this study, researchers identified RLTR45-int subfamily of ERVK as a strong reprogramming barrier for SCNT. This reprogramming barrier not only suppressed the expression of RLTR45-int elements, but also inhibited the expression of nearby genes, further affecting the development and survival of embryos.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Jin Sun, Weisheng Zheng, Wenqiang Liu, Xiaochen Kou, Yanhong Zhao, Zehang Liang, Lu Wang, Zihao Zhang, Jing Xiao, Rui Gao, Shaorong Gao, Cizhong Jiang
Summary: The study derived TSCs from blastocysts using different methods and analyzed the dynamics of transcription and DNA methylation, revealing the crucial role of FGF4 treatment in TSC formation. Specific DNA methylation remodeling processes and transcription factor networks play a key role in TSC pluripotency.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Lian Cai, Sang-Hwan Hyun, Eunhye Kim
Summary: This study reveals the important role of stem cell factor (SCF) in the development of porcine embryos and the establishment of embryonic stem cells (ESCs). SCF treatment has no significant effect on the development potential and blastocyst formation rate of both fertilized and cloned porcine embryos, but significantly increases the rate of hatched blastocysts in embryos cloned with Cloud eGFP as donor cells. The SCF/c-kit signaling pathway may play a pivotal role in embryo implantation. Moreover, SCF treatment significantly enhances the efficiency of deriving ESCs.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Yun Feng, Xin Zhao, Zhengda Li, Chan Luo, Ziyun Ruan, Jie Xu, Penglei Shen, Yanfei Deng, Jianrong Jiang, Deshun Shi, Fenghua Lu
Summary: The study found that KDM4D can regulate the level of H3K9me3 in buffalo SCNT embryos, promoting developmental competence and increasing the expression levels of ZGA and pluripotency-related genes.
MICROSCOPY AND MICROANALYSIS
(2021)
Article
Engineering, Biomedical
Stephanie E. Schneider, Adrienne K. Scott, Benjamin Seelbinder, Courtney Van Den Elzen, Robert L. Wilson, Emily Y. Miller, Quinn I. Beato, Soham Ghosh, Jeanne E. Barthold, Jason Bilyeu, Nancy C. Emery, David M. Pierce, Corey P. Neu
Summary: Cells are continuously influenced by dynamic environmental cues, especially mechanical cues, which affect their behavior and can lead to morphological and behavioral changes in the nucleus. The nucleus shows a responsive behavior to mechanical forces, propagating from the substrate, and disruption of this behavior can have lasting consequences on cellular functioning. Using an in vitro model of traumatic neural injury, the study demonstrates the dynamic nuclear behavioral response to impulse stretch and highlights the changes in cellular and nuclear behavior caused by mechanical forces.
ACTA BIOMATERIALIA
(2023)
Article
Computer Science, Interdisciplinary Applications
Qing Cheng, Pourya Delafrouz, Jie Liang, Chun Liu, Jie Shen
Summary: In this study, a special phase field/diffusive interface method was developed to model the nuclear architecture reorganization process, with a Lagrange multiplier approach used to preserve specific physical and geometrical constraints for biological events. Several efficient and robust linear and weakly nonlinear schemes were developed for this new model, with ample numerical simulations presented to validate the model and numerical methods, reproducing processes of nuclear architecture reorganization from experimental literature.
JOURNAL OF COMPUTATIONAL PHYSICS
(2022)
Article
Cell Biology
Ah Reum Lee, Ji-Hoon Park, Sung Han Shim, Kwonho Hong, Hyeonwoo La, Kyung-Soon Park, Dong Ryul Lee
Summary: The study found that the activity of Rad 51 is lower in SCNT eggs and a significantly lower level of DSBs in SCNT embryos during reprogramming. Supplementation with RS-1 during the activation of SCNT embryos can increase RAD51 expression and DSB foci, thereby improving the efficiency of SCNT reprogramming. Through single-cell RNA-seq analysis, reactivation of genes and involvement of histone demethylase in reducing reprogramming-resistant regions were observed, which may contribute to overcoming developmental block and enhancing cell sources for regenerative medicine.
CELL PROLIFERATION
(2021)
Article
Reproductive Biology
Reza Moradi-Hajidavaloo, Farnoosh Jafarpour, Mehdi Hajian, Mohsen Rahimi Andani, Shiva Rouhollahi Varnosfaderani, Mohammad Hossein Nasr-Esfahani
Summary: This study demonstrated that supplementation of IVC medium with 1.5 μM OAC1 can accelerate SCNT reprogramming and improve the quality of derived blastocysts.
Review
Genetics & Heredity
Zhenping Chen, Xuepeng Chen
Summary: The 3D chromatin structure plays a crucial role in gene expression regulation and embryonic development. Recent advancements in low-input chromatin conformation capture technologies have allowed for the study of 3D chromatin structures in gametes, zygotes, and early embryos in various species. The reorganization of 3D chromatin structure during embryogenesis shows both conserved and divergent patterns across different species, with distinct molecular mechanisms. These findings contribute to our understanding of the contribution of 3D chromatin structure reorganization to embryonic development and its role in acquiring totipotent developmental potential.
Article
Agriculture, Dairy & Animal Science
Tien Dat Nguyen, Honghui Li, Yiquan Zhuang, Bowei Chen, Keiji Kinoshita, Muhammad Ameen Jamal, Kaixiang Xu, Jianxiong Guo, Deling Jiao, Kumiko Tanabe, Yunfang Wei, Zhuo Li, Wenming Cheng, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei
Summary: This study successfully established an Asian elephant's fibroblast cell lines and performed interspecies somatic cell nuclear transfer with porcine oocytes. The reconstructed embryos showed in vitro development but failed to develop in vivo. Further investigation is needed to understand the reasons behind the in vivo developmental failure.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aline Acke, Siska Van Belle, Boris Louis, Raffaele Vitale, Susana Rocha, Thierry Voet, Zeger Debyser, Johan Hofkens
Summary: Interactions between epigenetic readers and histone modifications are crucial in gene expression regulation. We have developed a method called Expansion Microscopy for Epigenetics (ExEpi) that preserves spatial information and improves resolution, allowing the study of epigenetic interactions and drug discovery.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Takehiro Himaki, Kazuki Hano
Summary: This study investigates the effects of alpha lipoic acid (ALA) treatment during in vitro maturation (IVM) on porcine somatic cell nuclear transfer (SCNT) embryo production. The results show that treatment with 12.5μM ALA during IVM can improve porcine SCNT embryo development and quality.
ANIMAL SCIENCE JOURNAL
(2023)
Review
Cell Biology
Hongxin Zhong, Jie Zhang, Yuli Lu, Gongcheng Hu, Guangjin Pan, Hongjie Yao
Summary: The nucleosome is the basic subunit of chromatin, formed by histone octamers and genomic DNA. By folding and compressing, these structures form the 30-nm chromatin fiber, which is further organized within the nucleus in a hierarchical manner, known as the 3D genome. Understanding chromatin structure and regulatory changes in chromatin is crucial for studying cellular architecture, function, cell fate determination, regeneration, and diseases.
CELL PROLIFERATION
(2023)
Article
Reproductive Biology
Satoshi Kamimura, Kimiko Inoue, Eiji Mizutani, Jin-Moon Kim, Hiroki Inoue, Narumi Ogonuki, Kei Miyamoto, Shunya Ihashi, Nobuhiko Itami, Teruhiko Wakayama, Akihiro Ito, Norikazu Nishino, Minoru Yoshida, Atsuo Ogura
Summary: The study found that using chlamydocin analogues, which specifically inhibit I and IIa class HDACs, can improve the efficiency of mouse SCNT, with Ky-9 showing similar results to trichostatin A. The one-cell stage was identified as a critical period for enhancing cloning through the use of HDAC inhibitors in SCNT.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Rikke Morrish, Kevin Ho Wai Yim, Stefano Pagliara, Francesca Palombo, Richard Chahwan, Nicholas Stone
Summary: The study uses immune B lymphocyte development model, combining confocal Raman microscopy with transcriptomics to assess chromatin and transcriptional changes, linking changes in chemical and structural properties to biological outcomes. The results show that spectral analysis provides a powerful tool for studying gene activation, complementing traditional molecular biology techniques.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Weisheng Zheng, Liping Wang, Wenteng He, Xinjie Hu, Qianshu Zhu, Liang Gu, Cizhong Jiang
Summary: Haploid embryonic stem cells (haESCs) have similar transcriptome profiles to naive pluripotent stem cells and exhibit a similar chromatin structure to embryonic stem cells (ESCs). However, haESCs lack a nucleosome depletion region found in ESCs and have different chromatin states in enhancers related to the regulation of the cell cycle. These findings suggest that haESCs have unique characteristics and mechanisms compared to differentiated cell types.
CELL PROLIFERATION
(2023)