Article
Chemistry, Medicinal
Sara La Manna, Marilisa Leone, Flavia Anna Mercurio, Daniele Florio, Daniela Marasco
Summary: Researchers have designed several peptidomimetics to mimic the function of SOCS3 protein, with one chimeric peptide showing anti-inflammatory and antioxidant properties. The length and position of the cyclic analogues were found to play a crucial role in their structure and function.
Review
Cell Biology
Mohamad Mahjoor, Golnaz Mahmoudvand, Simin Farokhi, Alireza Shadab, Mojtaba Kashfi, Hamed Afkhami
Summary: The JAK/STAT signaling cascade plays a crucial role in regulating cell growth and differentiation, as well as immune responses and viral pathogenesis.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Chemistry, Medicinal
Sara La Manna, Sara Fortuna, Marilisa Leone, Flavia A. Mercurio, Ilaria Di Donato, Rosa Bellavita, Paolo Grieco, Francesco Merlino, Daniela Marasco
Summary: This study aimed to optimize the peptide structure and improve the biological activity of KIR-SOCS1. By utilizing different techniques, the researchers found that the size of the cyclic structure was crucial for its interaction with JAK2, and substituting native residues with un-natural building blocks enhanced the stability and affinity. These findings contribute to increasing the structural knowledge required for the recognition of SOCS1/JAK2 and advancing towards the development of more drug-like compounds.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Hyunjung Park, Sangjik Lee, Jaehun Lee, Hyuk Moon, Simon Weonsang Ro
Summary: This review highlights the importance of the JAK/STAT pathway in hepatocellular carcinoma (HCC) and its association with factors such as chronic inflammation and cirrhosis. It also summarizes recent advancements in targeting the JAK/STAT pathway for HCC treatment, emphasizing the potential of this pathway as a promising avenue for therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Liang Wang, Yan Hu, Baohui Song, Yongjian Xiong, Jingyu Wang, Dapeng Chen
Summary: The JAK/STAT signaling pathway plays a crucial role in the regulation of inflammatory bowel disease by modulating different cells and intestinal immune responses, and potential JAK inhibitors have been identified. Phytochemicals may aid in the discovery of precursor compounds for novel JAK inhibitors.
INFLAMMATION RESEARCH
(2021)
Article
Chemistry, Medicinal
Sara La Manna, Laura Lopez-Sanz, Susana Bernal, Sara Fortuna, Flavia A. Mercurio, Marilisa Leone, Carmen Gomez-Guerrero, Daniela Marasco
Summary: This study investigated the cellular effects of a cyclized JAK2 inhibitor, PS5, mimicking the SOCS1 protein. Through structural modifications, high affinity candidates towards the JAK2 catalytic domain were produced, which demonstrated inhibitory effects on JAK-mediated signaling pathways. The research provides insights into the development of low-molecular-weight proteomimetics with improved cellular effects.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Endocrinology & Metabolism
Qingluo Zhou, Qun Ren, Linhui Jiao, Jishang Huang, Jun Yi, Jincai Chen, Jinliang Lai, Guanglin Ji, Tiansheng Zheng
Summary: Osteoarthritis is an age-related degenerative disease that is influenced by inflammation and immunity. The JAK/STAT pathway plays a significant role in the pathological development of OA and may serve as a therapeutic target.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Chemistry, Medicinal
Saeed Mohammadian Haftcheshmeh, Maliheh Abedi, Kazem Mashayekhi, Mohammad Javad Mousavi, Jamshid Gholizadeh Navashenaq, Asadollah Mohammadi, Amir Abbas Momtazi-Borojeni
Summary: This review discusses the modulatory effects of berberine (BBR) on various inflammatory signaling pathways in the immune system and explores its therapeutic efficacy and anti-inflammatory effects.
PHYTOTHERAPY RESEARCH
(2022)
Article
Environmental Sciences
Xin Yu, Linfeng Li, Matthew T. V. Chan, William Ka Kei Wu
Summary: Research suggests that there may be a correlation between herpes zoster and COVID-19 through similar subset of genes involved in cytokine-cytokine receptor interaction. Both diseases show augmented Th17 cell differentiation and IL-17 signaling.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Plant Sciences
Xinying Fu, Zhengji Sun, Qingyin Long, Wei Tan, Huang Ding, Xiaodan Liu, Lu Wu, Yang Wang, Wei Zhang
Summary: This study demonstrated that glycosides, the main active components of BYHWD, can inhibit atherosclerosis progression by alleviating atherosclerotic inflammation, with the inhibitory mechanism targeting the activation of the JAK/STAT signaling pathway.
Article
Chemistry, Physical
Khaled Mukaddam, Sabrina Ruggiero, Steffen M. Berger, Dietmar Cholewa, Sebastian Kuhl, Daniel Vegh, Michael Payer, Michael M. Bornstein, Farah Alhawasli, Elizaveta Fasler-Kan
Summary: Zirconia implants have become widely used in dentistry due to their superior biological compatibility compared to traditional titanium implants, as they reduce bacterial adhesion, inflammation, and cellular interaction. This study investigated the activation of the JAK-STAT signaling pathway in osteoblast-like cells (MG-63) on zirconia and titanium surfaces using different cytokines. The results showed that various cytokines activated different STAT proteins on both surfaces, indicating the activation of the JAK-STAT pathway. The upregulation of MHC class I and class II proteins was observed when MG-63 cells were incubated on zirconia and titanium discs.
Article
Immunology
Lan Wang, Danfeng Zhao, Huan Wang, Lele Wang, Xiaohui Liu, Haiyan Zhang
Summary: FPS-ZM1 was found to inhibit LPS-induced microglial inflammation, with the possible mechanism involving the JAK/STAT signaling pathway. The study demonstrated the anti-inflammatory effects of FPS-ZM1 in both in vivo and in vitro models.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Veterinary Sciences
Shu-ming Chen, Ali Raza Jahejo, Fazul Nabi, Shakeel Ahmed, Jin-feng Zhao, Jin Yu, Chen-liang Zhang, Guan-bao Ning, Ding Zhang, Sayed Haidar Abbas Raza, Wen-xia Tian
Summary: This study investigated the Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway-related genes in thiram-induced tibial dyschondroplasia (TD) in broiler chickens. The findings suggest that differential expression of STAT3 and SOCS3 may play a crucial role in regulating abnormal proliferation, differentiation, or apoptosis of chondrocytes in the early stages of TD. Various molecular and histological analyses showed significant changes in gene expression and protein localization of STAT3 and SOCS3 in thiram-induced TD chickens, indicating their involvement in the pathogenesis of TD.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Clinical Neurology
Y. Nozari, C. Park, E. Brietzke, M. Iacobucci, H. Gill, R. McIntyre
Summary: Determining the role of changes in leptin signaling on cognitive function after bariatric surgery can aid in the development of therapeutic approaches targeting cognitive dysfunction in obese individuals. Although several studies have explored cognition post bariatric surgery and leptin changes, the integration of leptin signaling effects in relation to cognition post surgery has not been considered as a key aspect. This review highlights potential correlations and proposes the improvement of cognitive function through the amelioration of the leptin Janus kinase/Signal transducer and activator of transcription (JAK/STAT) signaling pathway by reversing inflammatory processes.
JOURNAL OF AFFECTIVE DISORDERS
(2023)
Article
Medicine, General & Internal
Ali Razaghi, Attila Szakos, Marwa Alouda, Bela Bozoky, Mikael Bjornstedt, Laszlo Szekely
Summary: Critically ill COVID-19 patients with pleural effusion have a higher risk of longer hospitalization, multisystem inflammatory syndrome, and mortality. The study evaluated the pleural effusions of deceased COVID-19 patients and identified elevated levels of various protein markers, indicating overstimulation of the immune system and cytokine storm. The study also found damage to arterial and cardiovascular organs. Certain markers, such as CA12, CD40, IL-6, IL-8, PD-L1, and VEGFA, showed potential as biomarkers for disease severity and prognosis or as therapy targets. Additionally, the involvement of JAK/STAT pathways in hyperinflammation suggests the potential use of JAK inhibitors as an effective treatment.
Article
Biochemistry & Molecular Biology
Abimbola Feyisara Adedeji Olulana, Miguel A. Soler, Martina Lotteri, Hendrik Vondracek, Loredana Casalis, Daniela Marasco, Matteo Castronovo, Sara Fortuna
Summary: The study introduces a rationally designed nanochip capable of capturing a target protein using computationally evolved peptides. Through nanopatterning the surface, the study successfully controlled the specific capture of peptides for the protein, demonstrating the feasibility of this approach in peptide design.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sara La Manna, Daniele Florio, Ilaria Iacobucci, Fabiana Napolitano, Ilaria De Benedictis, Anna Maria Malfitano, Maria Monti, Mauro Ravera, Elisabetta Gabano, Daniela Marasco
Summary: The effects of three platinum complexes on the aggregation of an amyloid model system were investigated, showing the ability of these compounds to repress amyloid aggregation and interact with the ligand field of Aβ peptide. Spectroscopic data and circular dichroism studies revealed that Pt-terpy induces soluble β-structures of monomeric Aβ(21-40) and reduces self-recognition, ultimately reducing cytotoxicity in human neuroblastoma cells. These findings suggest the potential application of metal-based agents as neurodrugs for neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Serena D'Agostino, Elisa Mazzega, Katja Pracek, Sara Piccinin, Flavia Pivetta, Michela Armellin, Sara Fortuna, Roberta Maestro, Ario de Marco
Summary: The study showed that interfering with the p53:Twist1 complex could alleviate Twist1's inhibitory effect on p53, providing potential therapeutic tools for p53 wild type tumors. Using VHH nanobodies targeting the p53 C-terminal region, the research demonstrated the possibility of this intervention and validated its activity in human cell models. These findings provide insights into p53 biology and the development of targeted therapeutic approaches.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Nadja Anneliese Ruth Ring, Maria Concetta Volpe, Tomaz Stepisnik, Maria Grazia Mamolo, Pane Panov, Dragi Kocev, Simone Vodret, Sara Fortuna, Antonella Calabretti, Michael Rehman, Andrea Colliva, Pietro Marchesan, Luca Camparini, Thomas Marcuzzo, Rossana Bussani, Sara Scarabellotto, Marco Confalonieri, Tho X. Pham, Giovanni Ligresti, Nunzia Caporarello, Francesco S. Loffredo, Daniele Zampieri, Saso Dzeroski, Serena Zacchigna
Summary: This study used machine learning analysis to virtually screen millions of compounds and identified new anti-fibrotic drugs that inhibit myofibroblast differentiation. Dopamine and its derivative TS1 were found to be effective inhibitors of myofibroblast activation, acting through dopamine receptor 3 and the transforming growth factor beta pathway. TS1 also had a preventive and reversing effect on lung fibrosis.
CELL DEATH & DISEASE
(2022)
Article
Chemistry, Multidisciplinary
Anamaria Todea, Sara Fortuna, Cynthia Ebert, Fioretta Asaro, Stefano Tomada, Marco Cespugli, Fabio Hollan, Lucia Gardossi
Summary: The lipase-catalyzed polycondensation of azelaic acid and glycerol was investigated using a Design-of-Experiment approach. The study provided rational guidelines for optimizing the reaction and successfully scaled it up for industrial use.
Review
Pharmacology & Pharmacy
Anna Procopio, Elena Lagreca, Rezvan Jamaledin, Sara La Manna, Brunella Corrado, Concetta Di Natale, Valentina Onesto
Summary: The study of novel drug delivery systems is an important area of biomedical research, which combines multi-disciplinary scientific approaches to improve the effectiveness of drugs. Biodegradable and bio-absorbable polymers are used as building blocks for these systems, and in silico-supported models are developed to optimize drug release.
Article
Chemistry, Medicinal
Sara La Manna, Marilisa Leone, Flavia Anna Mercurio, Daniele Florio, Daniela Marasco
Summary: Researchers have designed several peptidomimetics to mimic the function of SOCS3 protein, with one chimeric peptide showing anti-inflammatory and antioxidant properties. The length and position of the cyclic analogues were found to play a crucial role in their structure and function.
Article
Genetics & Heredity
Paola Borgia, Simona Baldassari, Nicoletta Pedemonte, Ebba Alkhunaizi, Gianluca D'Onofrio, Domenico Tortora, Elisa Cali, Paolo Scudieri, Ganna Balagura, Ilaria Musante, Maria Cristina Diana, Marina Pedemonte, Maria Stella Vari, Michele Iacomino, Antonella Riva, Roberto Chimenz, Giuseppe D. Mangano, Mohammad Hasan Mohammadi, Mehran Beiraghi Toosi, Farah Ashrafzadeh, Shima Imannezhad, Ehsan Ghayoor Karimiani, Andrea Accogli, Maria Cristina Schiaffino, Mohamad Maghnie, Miguel Angel Soler, Karl Echiverri, Charles K. Abrams, Pasquale Striano, Sara Fortuna, Reza Maroofian, Henry Houlden, Federico Zara, Chiara Fiorillo, Vincenzo Salpietro
Summary: This study reports the clinical and genetic spectrum of PEX13-related ZSDs and highlights the contribution of various disease mechanisms, including secondary mitochondrial dysfunction, to PEX13-related clinical phenotypes.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Chemistry, Medicinal
Daniele Zampieri, Sara Fortuna, Maurizio Romano, Emanuele Amata, Maria Dichiara, Agostino Marrazzo, Lorella Pasquinucci, Rita Turnaturi, Maria Grazia Mamolo
Summary: A small library of aminopropylcarboxamide derivatives was designed and synthesized from the deconstruction of previously synthesized compounds, showing the best results with benzofuran and quinoline derivatives with high affinity for both receptor subtypes. Some compounds exhibited high selectivity for the S2R subtype, particularly the quinoline derivative with a 35-fold higher affinity. The cytotoxic activity of these compounds was evaluated against breast cancer cell lines, with certain compounds showing comparable potency to existing medications. Molecular modeling confirmed the strong interaction of a specific compound with the S2R binding site, justifying its high affinity.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Daniele Zampieri, Sara Fortuna, Maurizio Romano, Alessandro De Logu, Gianluigi Cabiddu, Adriana Sanna, Maria Grazia Mamolo
Summary: We designed and synthesized a library of hydrazide derivatives to screen for novel antimycobacterial molecules. In vitro tests showed that five compounds had high activity against M. tuberculosis H37Ra attenuated strain and two compounds were effective against pyrazinamide-resistant strains. The compounds showed no antimycotic activity and low cytotoxicity against human cells. Molecular modeling suggested a plausible mechanism of action towards the active site of the InhA enzyme. The compounds were predicted to be potentially orally absorbed in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Daniela Benigno, Antonella Virgilio, Ivana Bello, Sara La Manna, Valentina Vellecco, Mariarosaria Bucci, Daniela Marasco, Elisabetta Panza, Veronica Esposito, Aldo Galeone
Summary: In this study, the biological properties of two TBA analogs (TBAG3 and TBAG4) and their derivatives were investigated. The results showed that these analogs exhibited anti-proliferative properties without anticoagulant activity. Among them, TBAG4 displayed excellent nuclease stability and antiproliferative properties against breast cancer cells binding NCL effectively, suggesting its potential as a promising therapeutic agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Daniele Florio, Sara La Manna, Concetta Di Natale, Marilisa Leone, Flavia Anna Mercurio, Fabiana Napolitano, Anna Maria Malfitano, Daniela Marasco
Summary: This study investigates the potential aggregation-prone regions in nucleophosmin (NPM)1 protein associated with acute myeloid leukemia (AML) through bioinformatic predictions and experimental structures. The findings reveal the existence of aggregation hot spots, mainly in the N-terminal domain (NTD) of the NPM1 protein, providing a more comprehensive view of the molecular details underlying NPMc+-dependent AML.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Ilaria Iacobucci, Sara La Manna, Irene Cipollone, Vittoria Monaco, Luisa Cane, Flora Cozzolino
Summary: Metals play crucial roles in organisms, and the dysregulation of metals can lead to various disorders. Recently, there has been growing interest in the study of metal-drug complexes, and various approaches have been developed to investigate and improve the molecular mechanisms and delivery efficiency of metal drugs.
Meeting Abstract
Clinical Neurology
E. Cali, C. Rocca, Y. Sahin, A. Al Shamsi, K. Mankad, E. Galanaki, S. Efthymiou, R. Nardello, P. De Marco, M. Ruggieri, F. Zara, P. Striano, Y. Sahin, L. Al Gazali, M. Abi Warde, B. Gerard, C. Beetz, S. Fortuna, M. Soler, E. Valente, R. Maroofian, V. Salpietro, H. Houlden
EUROPEAN JOURNAL OF NEUROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Sara La Manna, Concetta Di Natale, Valentina Onesto, Daniela Marasco
Summary: Self-assembling peptides are a novel class of agents with versatile applications in the biomedical field. They exhibit biocompatibility and molecular recognition capabilities, making them suitable for drug delivery and tissue engineering purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
