Article
Microbiology
Vanessa B. Vogensen, Mathieu S. Bolhuis, Marieke G. G. Sturkenboom, Tjip S. van der Werf, Wiel C. M. de Lange, Richard M. Anthony, Dick van Soolingen, Jan-Willem Alffenaar, Huib A. M. Kerstjens, Onno W. Akkerman
Summary: This retrospective study in a Dutch TB center investigated the impact of rifampicin on moxifloxacin exposure in patients with isoniazid-resistant or -intolerant TB. The study found a clinically significant 39% decrease in moxifloxacin exposure when rifampicin was co-administered, but moxifloxacin dose adjustment may compensate for this drug-drug interaction.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Medicine, General & Internal
Emily A. Kendall, Hamidah Hussain, Amber Kunkel, Rachel W. Kubiak, Anete Trajman, Richard Menzies, Paul K. Drain
Summary: The study found that using symptom-based screening for TB before initiating TPT can effectively reduce the occurrence of active TB cases, but may lead to an increase in drug resistance. Screening for subclinical TB can eliminate this increase in drug resistance, but if the screening requirement reduces access to TPT interventions, it may decrease the overall impact of TB prevention.
Article
Immunology
Andrew D. McCallum, Henry E. Pertinez, Aaron P. Chirambo, Irene Sheha, Madalitso Chasweka, Rose Malamba, Doris Shani, Alex Chitani, Jane E. Mallewa, Jamilah Z. Meghji, Jehan F. Ghany, Elizabeth L. Corbett, Stephen B. Gordon, Geraint R. Davies, Saye H. Khoo, Derek J. Sloan, Henry C. Mwandumba
Summary: This study explores the relationship between intrapulmonary drug levels and sputum bacillary clearance in tuberculosis treatment. The results show that higher drug exposure to rifampicin or isoniazid in the epithelial lining fluid is associated with more rapid bacillary elimination. Higher doses of rifampicin and isoniazid may result in sustained high intrapulmonary drug exposure, rapid bacillary clearance, shorter treatment duration, and better treatment outcomes.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Margaretha de Vos, Lesley Scott, Anura David, Andre Trollip, Harald Hoffmann, Sophia Georghiou, Sergio Carmona, Morten Ruhwald, Wendy Stevens, Claudia M. Denkinger, Samuel G. Schumacher
Summary: This study compared four automated assays for the detection of TB and MDR-TB and found that assays targeting multicopy genes or targets (Abbott, BD, and Roche) showed increased analytical sensitivity compared to Xpert. All assays demonstrated accuracy comparable to that of Genotype MTBDRplus for rifampicin and isoniazid resistance detection.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Oswaldo Hernandez-Gonzalez, Sergio Zarazua, Jose Ignacio Veytia-Bucheli, Marco Martin Gonzalez-Chavez, Cristian Jazmin Rodriguez-Pinal, Susanna Edith Medellin-Garibay, Edith Elena Uresti-Rivera, Francisco Javier Perez-Vazquez, Diana Patricia Portales-Perez, Silvia Romano-Moreno, Rosa del Carmen Milan-Segovia
Summary: This study developed and validated an alternative high-performance liquid chromatography method for simultaneous quantification of four antituberculosis drugs in plasma of patients. The method showed linearity, precision, and accuracy, and could be applied for therapeutic drug monitoring and pharmacokinetics studies throughout tuberculosis treatment.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Microbiology
Afsatou Ndama Traore, Mpumelelo Casper Rikhotso, Marry Avheani Mphaphuli, Sana Mustakahmed Patel, Hafsa Ali Mahamud, Leonard Owino Kachienga, Jean-Pierre Kabue, Natasha Potgieter
Summary: This review and meta-analysis investigated the prevalence and molecular insights into isoniazid (INH) and rifampicin (RIF) resistance-conferring mutations in Mycobacterium tuberculosis isolates from South Africa. High prevalence of specific mutations, including S450L in rpoB and S315T, linked to resistance against RIF and INH respectively, were found. These findings contribute to understanding drug resistance mechanisms and provide valuable insights for targeted interventions against drug-resistant TB.
Article
Neurosciences
Xiaoyu Qu, Jie Ma, Huan Gao, Yueming Zhang, Jinghui Zhai, Jiawei Gong, Yanqing Song, Tingting Hu
Summary: This study used metabolomics and proteomics analysis to investigate the neurotoxicity caused by the co-administration of isoniazid and rifampicin in tuberculosis treatment. The results showed dysregulation of several metabolic pathways and disruption in energy metabolism, particularly in the PPAR pathways. Activation of PPAR-alpha and PPAR-gamma may be a key target for alleviating the neurotoxicity induced by isoniazid and rifampicin.
Article
Immunology
Geetha Ramachandran, A. K. Hemanth Kumar, T. Kannan, Balamugesh Thangakunam, Deepa Shankar, Devasahayam J. Christopher
Summary: This preliminary study aimed to compare the pharmacokinetics of rifampicin, isoniazid, and pyrazinamide in TB patients receiving daily and thrice-weekly treatment regimens. The results showed that peak concentration of rifampicin was higher and peak concentration of isoniazid was lower in the daily dosing group. This suggests that the dose of isoniazid may need to be increased in a daily regimen. Rating: 6/10.
INDIAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Infectious Diseases
O. W. Akkerman, R. D. C. Dijkwel, H. A. M. Kerstjens, T. S. van der Werf, S. Srivastava, M. G. G. Sturkenboom, M. S. Bolhuis
Summary: This study describes the pharmacokinetics of rifampicin (RIF) and isoniazid (INH) in patients with drug-susceptible tuberculosis in a real-world setting. The results show that more than 50% of patients had drug exposure below the pre-specified target values, highlighting the need for better justification of drug exposure targets.
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
(2023)
Article
Pharmacology & Pharmacy
Laura Sibley, Andrew D. White, Charlotte Sarfas, Jennie Gullick, Fergus Gleeson, Faye Lanni, Simon Clark, Emma Rayner, Santiago Ferrer-Bazaga, Fatima Ortega-Muro, Laura Alameda, Joaquin Rullas, Veronica Sousa, Marisa Martinez, Inigo Angulo-Barturen, Adolfo Garcia, Juan Jose Vaquero, Henry E. Pertinez, Geraint Davies, Mike Dennis, Ann Williams, Sally Sharpe
Summary: Innovative cross-over study designs were explored in non-human primate studies to evaluate the effectiveness of drug treatments for tuberculosis. The pharmacokinetics of standard tuberculosis drugs were established in macaques, and two studies were conducted to evaluate the pharmacokinetics and pharmacodynamics of different drug combinations using cross-over designs. The studies supported the utility of the non-human primate model for determining the pharmacokinetics and pharmacodynamics of tuberculosis drugs, but further optimization of cross-over study designs is needed.
Article
Infectious Diseases
Tsung-Lun Li, Tai-Hua Chan, Cheng-Hui Wang, Ruwen Jou, Ming-Chih Yu, Denise Utami Putri, Chih-Hsin Lee, Yi-Hsien Lin
Summary: This case report highlights the importance of appropriate treatment and prompt diagnosis in eliminating tuberculosis. The patient initially diagnosed with latent tuberculosis infection later developed active TB disease with drug resistance, but showed improvement and no recurrence after individualized anti-TB treatment for 20 months. The study suggests that traditional diagnostic methods may not be sufficient to differentiate different stages of TB or LTBI.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
N. Palani, M. Premkumar, V. Vaishnavee, V. Dinesh, K. Thiruvengadam, J. Lavanya, Kaleeswari, R. Sridhar, A. Frederick, G. Sivaramakrishnan, R. Mondal, C. Padmapriyadarsini, S. Shanmugam
Summary: This study found that decentralised drug susceptibility testing at the district level has led to a decrease in rifampicin and isoniazid resistance in India. The increase in low-level inhA-c-15t mutation suggests a possible association with ethionamide/prothionamide resistance.
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
(2022)
Article
Infectious Diseases
Vichita Ok, Alexandra Aubry, Florence Morel, Isabelle Bonnet, Jerome Robert, Wladimir Sougakoff
Summary: The automated MDR/MTB ELITe MGB(R) assay demonstrated high sensitivity and specificity in detecting TB and associated drug resistance mutations in clinical samples. There was good agreement with genotypic drug susceptibility testing for the mutations tested, indicating the potential utility of this assay in rapid detection of pre-MDR and MDR-TB.
Article
Microbiology
Karla M. Tamez-Torres, Norma Mongua-Rodriguez, Leticia Ferreyra-Reyes, Pedro Torres-Gonzalez, Guadalupe Delgado-Sanchez, Maribel Martinez-Hernandez, Miriam Bobadilla-del-Valle, Velma Y. Jasso-Sosa, Priscila del S. Lopez-Castillo, Elizabeth Ferreira-Guerrero, Luis Pablo Cruz-Hervert, Jose Sifuentes-Osornio, Carlos A. Aguilar-Salinas, Lourdes Garcia-Garcia, Alfredo Ponce-de-Leon
Summary: Tuberculosis (TB) associated with diabetes mellitus (DM) is a growing problem, particularly in low- and medium-resource countries. However, the use of isoniazid (INH) or rifampicin (RIF) for TB preventive treatment (TPT) in people with type 2 DM is not safe enough to be considered a universal indication.
Article
Infectious Diseases
Saba Kabir, Kashaf Junaid, Abdul Rehman
Summary: Retreated cases are more prone to developing hot spot mutations, with an unusual difference in mutation patterns between drug naive and recurrence cases. Some mutations are exclusively associated with retreatment of anti-TB drugs, suggesting an increased risk of resistance with poor treatment outcomes.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Pharmacology & Pharmacy
Ilse Du Preez, Du Toit Loots
DRUG METABOLISM REVIEWS
(2018)
Review
Immunology
Ilse du Preez, Laneke Luies, Du Toit Loots
Article
Biochemical Research Methods
Ray-Dean Pietersen, Ilse du Preez, Du Toit Loots, Mari van Reenen, Derylize Beukes, Gina Leisching, Bienyameen Baker
JOURNAL OF MICROBIOLOGICAL METHODS
(2020)
Review
Microbiology
Laneke Luies, Ilse du Preez
CLINICAL MICROBIOLOGY REVIEWS
(2020)
Article
Chemistry, Medicinal
Monique Opperman, Du Toit Loots, Mari van Reenen, Katharina Ronacher, Gerhard Walzl, Ilse du Preez
Summary: Comparison of urinary metabolite profiles between eventually cured TB patients and those with treatment failure revealed similar patterns of metabolite fluctuations over time in both groups, with a delayed or shifted pattern observed in the successfully treated patients. Early onset of observed trends in the failed patients may be attributed to genotypic and phenotypic variations in drug metabolizing enzymes.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Multidisciplinary
Jano Jacobs, Cornelia Gertina Catharina Elizabeth van Sittert, Lodewyk Japie Mienie, Marli Dercksen, Monique Opperman, Barend Christiaan Vorster
Summary: This study elucidated the mechanism of formation of N-acylated amino acid (N-AAA) conjugates in urine and confirmed their non-enzymatic origin. The use of chiral strategy and molecular modelling was highlighted as important in investigating unknown constituents in biological samples. Further investigation of these conjugates is warranted to understand their potential role in maple syrup urine disease (MSUD) and other inborn errors of metabolism (IEMs).
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Tania Grobler, Monique Opperman, Janette Bester, Albe Carina Swanepoel, Ilse du Preez
Summary: This study comprehensively profiled the serum metabolome of participants using a combination of drospirenone (DRSP) and ethinyl estradiol (EE) containing oral contraceptives (COCs). The analysis revealed differences in metabolites between COC users and controls, suggesting a potential link to prothrombotic state and increased oxidative stress.
Article
Biotechnology & Applied Microbiology
Albe Carina Swanepoel, Janette Bester, Odette Emmerson, Prashilla Soma, Derylize Beukes, Mari van Reenen, Du Toit Loots, Ilse du Preez
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Monique Combrink, Ilse du Preez, Katharina Ronacher, Gerhard Walzl, Du Toit Loots
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2019)
Review
Medicine, Research & Experimental
Laneke Luies, Ilse du Preez, Du Toit Loots
BIOMARKERS IN MEDICINE
(2017)
Review
Medicine, Research & Experimental
Ilse du Preez, Laneke Luies, Du Toit Loots
BIOMARKERS IN MEDICINE
(2017)
