Article
Developmental Biology
Jennifer Shannon, Aishwarya Sundaresan, Orhan Bukulmez, Zexu Jiao, Kaitlin Doody, Sarah Capelouto, Bruce Carr, Laura A. Banaszynski
Summary: Understanding how gene regulatory elements influence ovarian follicle development is crucial for addressing issues such as decreased fertility with age and short-term graft function after ovarian tissue cryopreservation. The ATAC-seq technique can identify regulatory elements important for gene regulation and fertility, but challenges remain in obtaining data from frozen samples.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Endocrinology & Metabolism
Xiangyan Ruan, Jiaojiao Cheng, Juan Du, Fengyu Jin, Muqing Gu, Yanglu Li, Rui Ju, Yurui Wu, Huanmin Wang, Wei Yang, Haiyan Cheng, Long Li, Wenpei Bai, Weimin Kong, Xin Yang, Shulan Lv, Yuejiao Wang, Yu Yang, Xin Xu, Lingling Jiang, Yanqiu Li, Alfred O. Mueck
Summary: This study investigates the effectiveness and feasibility of ovarian tissue cryopreservation (OTC) in children facing gonadotoxicity treatment. The results suggest that OTC is a safe and effective method for preserving the fertility of prepubertal girls, and chemotherapy does not affect the effectiveness of OTC.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Adriana Carvalho Natal de Moraes, Hamideh Pourmohammadi Fallah, Valeria Freitas de Magalhaes, Hamid R. Habibi
Summary: This study investigates the direct effects of cylindrospermopsin (CYN) on female reproduction in zebrafish. The results show that CYN increases germinal vesicle breakdown, caspase-3 activity, and testosterone production, while decreasing the abundance of specific transcripts. These findings provide insights into the adverse effects of CYN on female reproduction and offer potential biomarkers for CYN exposure.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Genetics & Heredity
Hu Tao, Juan Yang, Pengpeng Zhang, Nian Zhang, Xiaojun Suo, Xiaofeng Li, Yang Liu, Mingxin Chen
Summary: In this study, 895 lncRNAs were identified in the pre-ovulatory ovarian follicles of goats, with 88 lncRNAs showing differential expression between Macheng black goat and Boer goat. Additionally, a sponge lncRNA was identified.
FRONTIERS IN GENETICS
(2022)
Article
Environmental Sciences
Ramses Santacruz-Marquez, Jodi A. Flaws, Luz del Carmen Sanchez-Pena, Isabel Hernandez-Ochoa
Summary: The use of zinc oxide nanoparticles may potentially affect the functions of ovarian antral follicles, leading to increased estrogen production and an antioxidant response.
Article
Agriculture, Dairy & Animal Science
Risu Na, Yan Zeng, Yanguo Han, Chengli Liu, Baigao Yang, Yongmeng He
Summary: The mechanism of multiple ovulations in prolific goats remains unclear, but this study found that nine miRNAs were differently expressed between ovulatory and subordinate follicles in Dazu black goats. Chi-miR-130b-5p and chi-miR-214-3p were predicted to regulate genes involved in gonadotropin hormone signaling and oocyte-derived growth factors. Further studies are needed to confirm these findings.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Takashi Yoshino, Takahiro Suzuki, Go Nagamatsu, Haruka Yabukami, Mika Ikegaya, Mami Kishima, Haruka Kita, Takuya Imamura, Kinichi Nakashima, Ryuichi Nishinakamura, Makoto Tachibana, Miki Inoue, Yuichi Shima, Ken-ichirou Morohashi, Katsuhiko Hayashi
Summary: Novel methods have been developed to generate functional mouse oocytes from pluripotent stem cell-derived primordial germ cell-like cells (PGCLCs) and fetal ovarian somatic cell-like cells (FOSLCs). When FOSLCs are aggregated with PGCLCs, they enter meiosis and produce oocytes capable of fertilization and development into live offspring, providing a method for in vitro oocyte production and follicle generation for a better understanding of mammalian reproduction.
Article
Agriculture, Dairy & Animal Science
Weijing Zhang, Lingjiang Min, Yajing Li, Yaning Lang, S. A. Masudul Hoque, Adedeji Olufemi Adetunji, Zhendong Zhu
Summary: In this study, it was found that supplementation of 2 mM proline significantly improved post-thaw goat sperm quality by regulating sperm redox homeostasis. The addition of proline protected goat sperm against reactive oxygen species (ROS) stress through PRODH-mediated metabolism. These findings suggest that proline can be used as an effective additive to enhance post-thaw goat sperm quality during cryopreservation.
Article
Biology
Weijing Zhang, Haixiang Cui, Kexin Ding, Kaifeng Zhou, Yajing Li, S. A. Masudul Hoque, Lingjiang Min, Zhendong Zhu
Summary: This study investigated the effect of the novel cryoprotectant CPLL on the post-thaw quality of goat cryopreserved sperm. It was found that the addition of CPLL improved the motility, membrane and acrosome integrity, mitochondrial function, ATP production, and antioxidant defense mechanisms of the post-thaw sperm. Furthermore, CPLL supplementation reduced apoptosis, MDA levels, and ROS production. These findings highlight the potential of CPLL in maintaining sperm quality and antioxidant activity during cryopreservation.
Article
Cell Biology
Huan Yi, Huamin Wu, Wenbin Zhu, Qi Lin, Xiaoyan Zhao, Rong Lin, Yan Luo, Lixiang Wu, Danmei Lin
Summary: The study aimed to explore the correlations between phthalates, common environmental endocrine disruptors, and ovarian dysfunction in endometriosis. The levels of phthalates in urine samples were significantly higher in women with endometriosis. In rat models, exposure to DEHP resulted in decreased ovarian and uterine mass index, irregular estrous cycle, and negatively correlated serum AMH levels. These findings suggest that phthalate exposure, especially DEHP, is associated with endometriosis and affects ovarian function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Environmental Sciences
Andressa Gonsioroski, Mary Laws, Vasiliki E. Mourikes, Alison Neff, Jenny Drnevich, Michael J. Plewa, Jodi A. Flaws
Summary: This study identified differentially expressed genes and pathways involved in the toxic effects of iodoacetic acid (IAA) on ovarian follicles. Exposure to IAA altered gene expression and decreased estradiol levels in mice.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2022)
Article
Veterinary Sciences
Bingbing Xu, Xue Bai, Jian Zhang, Boyuan Li, Yanjun Zhang, Rui Su, Ruijun Wang, Zhiying Wang, Qi Lv, Jiaxin Zhang, Jinquan Li
Summary: This study investigated the factors affecting sperm freezability in goat seminal plasma. Goats were divided into high-freezability (HF) and low-freezability (LF) groups based on the total motility of thawed sperm. The seminal plasma composition was found to be related to the differences in sperm motility and integrity. Metabolomic analysis revealed clear separations in metabolic patterns between the HF and LF groups. Differential metabolites, including L-glutamine, L-aspartate, L-arginine, phenylpyruvate, benzoic acid, ketoisocaproic acid, and choline, were identified as potential biomarkers for sperm freezability. These metabolites may directly affect sperm freezability or regulate anabolic processes in sperm.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Multidisciplinary Sciences
V. R. Hlokoe, T. L. Tyasi, B. Gunya
Summary: The study reviewed the development of ovarian follicles from small cortical follicles to hierarchical follicles, focusing on chicken ovarian follicles morphology and the expression of the GDF9 gene. The results showed that GDF9 gene expression plays a crucial role in follicular growth, stimulating the proliferation of granulosa cells in pre-hierarchical stages.
Article
Veterinary Sciences
Jiahao Zou, Lixuan Wei, Dexian Li, Yongtao Zhang, Guang Wang, Lei Zhang, Ping Cao, Shaohua Yang, Guang Li
Summary: Adding different concentrations of glutathione to the frozen diluent of dairy goat semen, it was found that the best quality of thawed sperm was achieved when the concentration of glutathione was 2 mmol/L, with significantly improved sperm viability, plasma membrane intact rate, and acrosome intact rate.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Endocrinology & Metabolism
Linn Salto Mamsen, Jane Alro Botkjaer, Stine Gry Kristensen, Susanne Elisabeth Pors, Janni Vikkelso Jeppesen, Ajay Kumar, Bhanu Kalra, Erik Ernst, Claus Yding Andersen
Summary: AMH, a member of the TGF-beta superfamily produced by follicular granulosa cells in women, is thought to inhibit aromatase and play a role in ovarian steroidogenesis. This study found a negative correlation between follicle diameter and AMH forms, and variability in AMH isoforms between follicles of identical size within the same woman. The complex intrafollicular processing of AMH may pose challenges for developing an antibody-based AMH assay.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Francisco Denilson Rodrigues Gomes, Danielle Cristina Calado de Brito, Naiza Arcangela Ribeiro de Sa, Lucy Vanessa Sulca Naupas, Gaby Judith Quispe Palomino, Renato Felix da Silva, Everton Pimentel Ferreira Lopes, Gildas Tetaping Mbemya, Benner Geraldo Alves, Mary Zelinski, Jose Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues
Summary: The study found that VSF can grow and develop during 12 days of in vitro culture, and showed evidence of preservation of steroidogenic function and remodeling of the basement membrane.
CELL AND TISSUE BANKING
(2022)
Article
Cell Biology
J. Cadenas, N. A. R. Sa, A. C. A. Ferreira, V. M. Paes, F. L. N. Aguiar, C. C. L. Fernandes, R. Rossetto, B. G. Alves, G. A. Apgar, D. M. Magalhaes-Padilha, D. Rondina, J. R. Figueiredo
Summary: This study evaluated the effects of whole flaxseed as a feed supplementation on the in vitro development of caprine early antral follicles and embryo production. The results showed that flaxseed did not affect follicular growth and diameter, but increased the recovery rate of oocytes and negatively affected sperm penetration rate.
Article
Reproductive Biology
Juliana Z. Almeida, L. A. Vieira, C. Maside, A. C. A. Ferreira, N. A. R. Sa, H. H. Correia, V. R. Araujo, R. S. Raposo, J. Smitz, C. C. Campello, J. R. Figueiredo, R. B. Oria
Summary: 5FU does not affect follicular morphology but detrimentally affects follicular growth, estradiol production, and oocyte maturation in isolated ovarian follicles. Concentrations of 5FU greater than 3 mM reduce estradiol levels.
Article
Reproductive Biology
A. D. Sales, A. B. G. Duarte, R. M. P. Rocha, I. R. Brito, Y. Locatelli, B. G. Alves, K. A. Alves, J. R. Figueiredo, A. P. R. Rodrigues
Summary: This study aimed to investigate the relative abundances of ABC transporters mRNA transcripts in ovine ovarian tissue after vitrification and in vitro culture. The results showed a negative correlation between degenerated follicles and mRNA transcript abundances of ABCB1 and ABCG2, with a significant decrease in the percentage of growing follicles after in vitro culture. Further studies are needed to explore the functions of ABC transporters in ovine folliculogenesis after in vitro culture.
Article
Cell Biology
D. C. C. Brito, I. P. Silva, A. C. A. Ferreira, N. A. R. Sa, M. I. F. Guedes, A. P. R. Rodrigues, R. R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of zearalenone and matairesinol on the morphology of in vitro-cultured ovarian follicles. Zearalenone impaired the follicle morphology, while the combination of matairesinol and zearalenone counteracted the negative effects. However, matairesinol did not promote follicle development in vitro.
Article
Agriculture, Dairy & Animal Science
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study characterized the major proteome of preimplantation ovine embryos grown in vitro. The analysis identified proteins that are involved in translation, protein transport, and stabilization. Functional cluster analysis revealed differences between the in vitro and in vivo proteomes of sheep embryos, indicating that in vitro derived blastocysts have distinct protein profiles compared to those produced in vivo.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Veterinary Sciences
R. F. Silva, L. F. Lima, Anna C. A. Ferreira, A. F. B. Silva, D. R. Alves, B. G. Alves, A. C. Oliveira, Selene M. Morais, Ana Paula R. Rodrigues, Regiane R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of different concentrations of eugenol, ascorbic acid, and anethole in goat ovarian tissue. The results showed that eugenol promoted follicle development and survival, and reduced oxidative stress. Furthermore, eugenol decreased gene methylation and endoplasmic reticulum stress.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Kendall A. Hyde, Francisco L. N. Aguiar, Paula B. Alvarenga, Amanda L. Rezende, Benner G. Alves, Kele A. Alves, Gustavo D. A. Gastal, Melba O. Gastal, Eduardo L. Gastal
Summary: This study investigated the clustering and neighborhood patterns of preantral follicles in the horse ovary, showing that younger mares have more clustering and neighbors compared to older mares. The clustering was found in all areas of the ovary, and follicles with neighbors were more likely to be morphologically normal.
Article
Reproductive Biology
A. F. B. Silva, L. F. Lima, A. N. P. Morais, L. L. Lienou, Y. F. Watanabe, D. C. Joaquim, S. M. Morais, D. R. Alves, A. F. Pereira, A. C. Santos, B. G. Alves, D. M. M. Padilha, E. L. Gastal, J. R. Figueiredo
Summary: This study investigates the effects of eugenol supplementation on bovine oocyte in vitro maturation and the subsequent in vitro embryo production and quality through IVF. The results show that adding 40μM eugenol to the maturation medium improves antioxidant capacity and cleavage rate. Additionally, lower calreticulin levels and higher total cell numbers in expanded blastocysts were observed in the lower eugenol concentration treatments. Based on these findings, enriching the maturation medium with 10μM eugenol is recommended for in vitro bovine embryo production.
Article
Agriculture, Dairy & Animal Science
Kamila S. Otavio, Jose R. S. Passos, Renato F. Silva, Laritza F. Lima, Jesus Cadenas, Victor M. Paes, Hudson H. Correia, Anna Clara A. Ferreira, Francisca G. Canafistula, Maria Julia B. Bezerra, Lais L. B. Oliveira, Guilherme G. C. Carvalho, Carlos R. K. Paier, Claudia Pessoa, Denise G. Guerreiro, Maria Elisabete A. Moraes, Jose Ricardo Figueiredo, Arlindo A. Moura
Summary: The study evaluated the proteome of early antral follicles from Ovis aries using LC-MS/MS analysis. A total of 2503 follicle proteins were identified, including abundant proteins such as vimentin, actin, lamin, heat shock proteins, and histones. miRNAs were found to modulate genes involved in cell cycle, cell differentiation, aging, apoptosis, adipocyte differentiation, and cell division in sheep follicles. This comprehensive atlas of proteins expressed in sheep early antral follicles will contribute to the identification of biomarkers for follicular development and oocyte maturation.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
L. V. S. Naupas, D. C. C. Brito, S. S. Souza, F. A. S. Brandao, R. F. Silva, C. C. L. Fernandes, G. M. Tetaping, B. G. Alves, J. Y. G. Silva, M. I. F. Guedes, D. Rondina, J. R. Figueiredo, A. P. R. Rodrigues
Summary: This study found that adding 100μM or 150μM ALA to the vitrification solution can protect sheep ovarian tissue, maintain follicle morphology, and promote follicular development after in vitro culture or autotransplantation. However, high levels of ALA may cause oxidative stress and tissue fibrosis after transplantation.
Article
Agriculture, Dairy & Animal Science
Luisa Miglio, Marco Aurelio Schiavo Novaes, Laritza Ferreira de Lima, Tatiane Moraes Arantes, Kele Amaral Alves, Francisca Elda Ferreira Dias, Marcelo Emilio Beletti, Jose Ricardo de Figueiredo
Summary: This study investigated the effects of zinc oxide nanoparticles functionalized with curcumin (ZnO(np) + CUR) on bovine in vitro embryo production and cellular antioxidant response during in vitro embryo culture (IVC). Cumulus-oocyte complexes (COCs) were matured, fertilized, and cultured in the presence of different concentrations of ZnO(np) + CUR. The results showed that the addition of 12μM ZnO(np) + CUR during IVC reduced intracellular ROS production and blastocyst production rate.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Cell Biology
A. N. P. Morais, L. F. Lima, A. F. B. Silva, L. L. Lienou, A. C. A. Ferreira, Y. F. Watanabe, D. C. Joaquim, B. G. Alves, A. F. Pereira, D. R. Alves, A. C. Oliveira, S. M. Morais, D. M. Magalhaes-Padilha, J. R. Figueiredo, E. L. Gastal
Summary: This study investigates the effects of carvacrol supplementation on in vitro maturation and embryo production in bovine oocytes. It was found that carvacrol supplementation increased the antioxidant capacity of the culture medium, but decreased the number of cells per blastocyst.
Article
Biochemistry & Molecular Biology
Ana F. B. Silva, Ana N. P. Morais, Laritza F. Lima, Anna C. A. Ferreira, Renato F. Silva, Naiza A. R. Sa, Satish Kumar, Ariclecio C. Oliveira, Benner G. Alves, Ana P. R. Rodrigues, Eduardo L. Gastal, Vilceu Bordignon, Jose R. Figueiredo
Summary: This study assessed the histones methylation profile in late preantral and early antral caprine follicles grown in vivo and in vitro, and the effect of anethole during in vitro culture. The results showed that in vitro culture affected histone methylation, mRNA abundance of apoptotic genes, and steroidogenic and demethylase enzymes in follicles compared with in vivo-grown follicles.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Reproductive Biology
Patrycja Kurowska, Ewa Mlyczynska, Julia Wajda, Konrad Krol, Karolina Pich, Patrycja Guzman, Aleksandra Greggio, Oliwia Szkraba, Malgorzata Opydo, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression of PNX-14 and GPR173 in ovarian tissue and the effects of PNX-14 on granulosa cell function. The results showed that PNX-14 can influence ovarian follicle growth by promoting granulosa cell proliferation, hormone secretion, and cell cycle progression.
REPRODUCTIVE BIOLOGY
(2024)
Article
Reproductive Biology
Sonia Srivastava, Swarnima Mishra, Gyan Babu, Banalata Mohanty
Summary: This study aimed to investigate the efficacy of PD149163 in preventing/ameliorating LPS-induced inflammation in the female reproductive system of mice. The results showed that PD149163 reduced/prevented the inflammation in the ovary and uterine horn, restored the balance of inflammatory cytokines and oxidative stress, and recovered the levels of reproductive hormones and corticosterone.
REPRODUCTIVE BIOLOGY
(2024)
Article
Reproductive Biology
Chen Zhang, Hongyan Cheng, Xue Ye, Heng Cui, Yi Li, Honglan Zhu, Xiaohong Chang
Summary: Extracellular matrix protein 1 (ECM1) is significantly upregulated in endometriosis tissues. Blocking ECM1 attenuates cell migration and invasion. ECM1 regulates cell migration, invasion, and cytoskeleton reorganization via the RhoC/ROCK1 signaling pathway.
REPRODUCTIVE BIOLOGY
(2024)
