Review
Agriculture, Dairy & Animal Science
Hiba Ali Hassan, Guillaume Domain, Gaia Cecilia Luvoni, Rana Chaaya, Ann Van Soom, Eline Wydooghe
Summary: Epididymal semen from dogs and cats provides an additional source of gametes for preserving valuable genetics, especially in cases where ejaculation is not possible, castration is necessary, or unexpected death occurs. Understanding the differences between epididymal semen and ejaculated semen is crucial for optimizing cryopreservation and enhancing the applications of assisted reproductive technologies. Factors affecting epididymal semen quality and freezability need to be identified and addressed to improve post-thaw semen quality and ART outcomes.
Article
Biology
Christelle Damon-Soubeyrand, Antonino Bongiovanni, Areski Chorfa, Chantal Goubely, Nelly Pirot, Luc Pardanaud, Laurence Piboin-Fragner, Caroline Vachias, Stephanie Bravard, Rachel Guiton, Jean-Leon Thomas, Fabrice Saez, Ayhan Kocer, Meryem Tardivel, Joel R. Drevet, Joelle Henry-Berger
Summary: The epididymis plays a crucial role in male fertility and has both secretory and immune functions. However, the organization of blood and lymphatic networks in the epididymis is still largely unknown.
Article
Genetics & Heredity
Elena Mele, Raffaella D'Auria, Marika Scafuro, Marianna Marino, Silvia Fasano, Andrea Viggiano, Riccardo Pierantoni, Antonietta Santoro, Rosaria Meccariello
Summary: The study investigated the expression of Kiss1 and Kiss1 receptor (Kiss1R) in rat spermatozoa and epididymis, revealing that they are present in both spermatozoa and the different regions of the epididymis. The findings suggest that the kisspeptin system may play an important role in the maturation and storage of spermatozoa in the epididymis.
Article
Reproductive Biology
Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie A. Walker, Robert A. Cushman, George A. Perry
Summary: Sperm stored in the epididymis have a longer lifespan, but their motility increases and lifespan decreases upon ejaculation. This study aimed to identify protein differences between epididymis and ejaculated samples that are associated with longevity. The analysis revealed that energy metabolism in the epididymis is more glycolytic and epididymal sperm have more antioxidants, which may contribute to maintaining sperm in a quiescent state.
Article
Biochemistry & Molecular Biology
Christopher Casciaro, Hirotaka Hamada, Alisa Kostaki, Stephen G. Matthews
Summary: Approximately 1%-3% of the adult population are treated with sGCs, and previous studies have shown that pre-conception adversities in males may affect offspring's neuroendocrine function and behavior through miRNA changes in sperm. This study aimed to investigate the region-specific effects of sGC exposure on sperm miRNA profile in the epididymis. Results showed that Dex-exposure led to decreased expression of one miRNA in the caput and 31 miRNAs in the cauda. This indicates that sGCs target the epididymis to modify sperm miRNA but do not affect miRNA content during spermiation in the testes.
Article
Multidisciplinary Sciences
Ji-won Choi, Ludvik Alkhoury, Leonardo F. Urbano, Puneet Masson, Matthew VerMilyea, Moshe Kam
Summary: Computer-Assisted Semen Analysis (CASA) is a reliable technique for analyzing semen images, and it can process a large number of images with high consistency, accuracy, and repeatability. Simulation models of semen images enable objective assessments of CASA algorithms and can be used to study algorithms for segmentation, localization, and tracking of sperm cells.
SCIENTIFIC REPORTS
(2022)
Article
Veterinary Sciences
Josue Calderon-Calderon, Francisco Sevilla, Eduardo R. S. Roldan, Vinicio Barquero, Anthony Valverde
Summary: This study aimed to evaluate the effect of fat-soluble vitamin supplementation on the seminal quality of boars. The results showed that fat-soluble vitamin supplementation increased semen volume, motility, progressive motility, and velocity variables. Overall, dietary fat-soluble vitamin supplementation significantly improved the semen quality of boar ejaculates.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Fisheries
Daniella Aparecida de Jesus Paula, Naiara Melo, William Franco Carneiro, Pedro Leite de Castro Uzeda, Bruna Resende Chaves, Isaac Filipe Moreira Konig, Luis David Solis Murgas
Summary: This study aimed to develop an alternative protocol for freezing fish semen using an ultra-freezer. The results demonstrate that this method is efficient in maintaining semen quality.
AQUACULTURE INTERNATIONAL
(2022)
Article
Agriculture, Dairy & Animal Science
Anna Wysokinska, Ewa Wojcik, Angelika Chlopik
Summary: Sperm morphometry is an important indicator of the quality of spermatozoa. A study on dog epididymides found that sperm collected from different segments varied in size, with the cauda having the smallest dimensions. Staining methods affected sperm dimensions, with different fixatives and reagents leading to variations in head size.
Article
Developmental Biology
Brett Nixon, Amanda L. Anderson, Elizabeth G. Bromfield, Jacinta H. Martin, Tessa Lord, Shenae L. Cafe, Shaun D. Roman, David A. Skerrett-Byrne, Andrew L. Eamens, Geoffry N. De Iuliis, Stephen D. Johnston
Summary: This study investigated the morphology and histology of the male reproductive system of crocodiles, focusing on the role of the excurrent duct system in sperm production and modification. The findings suggest that the ducts are involved in seminal fluid production and/or modification, contributing to the nourishment, protection, and storage of crocodile sperm. The structural characteristics of the crocodile male excurrent duct system share closer similarities with birds than with other groups within the Reptilia class or Mammalia, reflecting their common Archosaurs ancestry.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Article
Biology
Giovanni Cilia, Fabrizio Bertelloni, Domenico Cerri, Filippo Fratini
Summary: Genital leptospirosis in wild boars is caused by Leptospira fainei, a intermediate strain not previously detected in reproductive organs of male wild boars. This finding provides new insights into the epidemiology of the disease and emphasizes the importance of further research in this area.
Article
Anatomy & Morphology
Ana Sofia Dip, Eduardo Martin, Ruben Aldo Andrada, Marcela Beatriz Hernandez, Maria Daniela Miotti
Summary: This study describes and compares the sperm morphology and morphometric features of Molossus molossus and Molossops temminckii, finding that the sperm of both bat species are practically identical morphologically and morphometrically. Scanning electron microscopy allowed visualization of structures, such as acrosomal vesicles, that were not detected with other types of microscopy.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Agriculture, Dairy & Animal Science
Pakpoom Navanukraw, Luis A. Lugo-Roman, Rawiwan Im-Erbsin, Junpen Suwimonteerabutr, Sroisuda Chotimanukul, Kaywalee Chatdarong
Summary: Equex STM paste improves post-thaw quality of rhesus monkeys' epididymal spermatozoa by enhancing sperm motility and acrosome integrity.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Biology
Cristina A. Martinez, Jordi Roca, Manuel Alvarez-Rodriguez, Heriberto Rodriguez-Martinez
Summary: This study investigated the presence and abundance of specific microRNA sequences in porcine spermatozoa and their relation to boar fertility. The study identified several miRNAs that were not previously reported in pigs and found differences in abundance of miRNAs known to affect fertility-related genes. These findings suggest that miRNAs could serve as fertility markers in artificial insemination programs.
Article
Biochemistry & Molecular Biology
Tricia Rowlison, Pierre Comizzoli
Summary: Key protein LGALS3BP is transferred via extracellular vesicles between the epididymis and maturing sperm cells, and this transfer affects sperm fertilizing ability and developmental potential. Inhibition of LGALS3BP leads to decreased fertilization success and slower first cell cycles, demonstrating the importance of LGALS3BP in sperm function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
F. T. N. M. A. Romao, P. F. V. Pereira, K. K. M. C. Flaiban, A. C. O. Dearo, T. M. Fernandes, J. A. N. Lisboa
EQUINE VETERINARY JOURNAL
(2017)
Article
Veterinary Sciences
Marco A. F. Lopes, Antonio C. O. Dearo, Allen Lee, Shannon K. Reed, Joanne Kramer, P. Frank Pai, Yoshiharu Yonezawa, Hiromitchi Maki, Terry L. Morgan, David A. Wilson, Kevin G. Keegan
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2016)
Article
Veterinary Sciences
F. C. Pinto, G. C. Bregadioli, L. A. Carvalho, A. L. Hasuda, A. C. O. Dearo, K. K. M. C. Flaiban, J. A. N. Lisboa
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2018)
Article
Agronomy
Peter Reichmann, Antnio Cezar de Oliveira Dearo, Tais Casonato Rodrigues
Article
Veterinary Sciences
A. C. O. Dearo, G. de Moraes Marcondes, J. C. O. Araujo, C. E. S. Grumadas, P. C. Marino, W. T. Teixeira, G. de La Penha Chiacchio Fernandes
EQUINE VETERINARY EDUCATION
(2014)
Article
Veterinary Sciences
Antonio Cezar de Oliveira Dearo, Carmen Esther Santos Grumadas, Peter Reichmann, Gustavo Rodrigues Queiroz, Marilu Constantino Max, Mariana Cosenza
JOURNAL OF EQUINE VETERINARY SCIENCE
(2008)
Article
Veterinary Sciences
Antonio Cezar de Oliveira Dearo, Roberta Garbelini Gomes, Rafael Goulart Araujo, Peter Reichmann, Mariana Cosenza, Mariana Marcantonio Coneglian
JOURNAL OF EQUINE VETERINARY SCIENCE
(2009)
Article
Agriculture, Multidisciplinary
Carlos Augusto Alves Melanda, Ana Beatriz Marques de Almeida, Myrian Megumy Tsunokawa Hidalgo, Anne Kemmer Souza, Luiz Guilherme Corsi Trautwein, Jamile Haddad Neta, Evelyn Rangel dos Santos, Antonio Cezar de Oliveira Dearo, Maria Isabel Mello Martins
Summary: The study aimed to evaluate the filtration method using a sperm-filter to separate seminal plasma of boars' ejaculate, based on sperm viability, hyperactivity, and lipid peroxidation levels. Results showed that the filtration method with BTS dilution maintained sperm viability while effectively separating seminal plasma. Total motility was higher in group G1 with fresh samples, and hyperactivity was observed in filtered semen at 48 and 96 hours after cooling. Additionally, lipid peroxidation levels were higher in group G1 compared to G2 and G3.
SEMINA-CIENCIAS AGRARIAS
(2021)
Article
Agronomy
Mariana Cosenza, Priscilla Fajardo Valente Pereira, Loraine Ines Fernandes, Antonio Cezar de Oliveira Dearo, Karina Keller Marques da Costa Flaiban, Julio Augusto Naylor Lisboa
Article
Agronomy
Antonio Cezar de Oliveira Dearo, Vitor Bruno Bianconi Rosa, Peter Reichmann, Milton Luis Ribeiro de Oliveira
Article
Agriculture, Multidisciplinary
Ana Victoria Zeppelini Nascimento, Amanda Queiroz de Carvalho, Antonio Cezar de Oliveira Dearo
SEMINA-CIENCIAS AGRARIAS
(2009)
Article
Veterinary Sciences
SG Pearce, AC Dearo, BE Howard, BA Brisson
AUSTRALIAN VETERINARY JOURNAL
(2003)
Article
Agriculture, Multidisciplinary
Antonio Cezar de Oliveira Dearo, Joandes Henrique Fonteque, Fabricio Delgado Vidal, Josmari Pirolo
SEMINA-CIENCIAS AGRARIAS
(2010)