Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm−oocyte fusion in mice

Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice

(2020) Yoshitaka Fujihara et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice†

(2019) Yonggang Lu et al.   BIOLOGY OF REPRODUCTION

Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice

(2019) Yoshitaka Fujihara et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Male mice, caged in the International Space Station for 35 days, sire healthy offspring

(2019) Takafumi Matsumura et al.   Scientific Reports

Sperm–egg interaction and fertilization: past, present, and future

(2018) Masaru Okabe   BIOLOGY OF REPRODUCTION

Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice

(2018) Yoshitaka Fujihara et al.   JOURNAL OF CELL SCIENCE

Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity†

(2018) Taichi Noda et al.   BIOLOGY OF REPRODUCTION

The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids

(2018) Brian P. Hermann et al.   Cell Reports

Structure of IZUMO1–JUNO reveals sperm–oocyte recognition during mammalian fertilization

(2016) Umeharu Ohto et al.   NATURE

Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex

(2016) Halil Aydin et al.   NATURE

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice

(2016) Haruhiko Miyata et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization

(2016) Kazuki Kato et al.   Nature Communications

CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice

(2016) Asami Oji et al.   Scientific Reports

Mechanisms of fertilization elucidated by gene-manipulated animals

(2015) Masaru Okabe   ASIAN JOURNAL OF ANDROLOGY

CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis

(2015) Samantha Young et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Oocyte-triggered dimerization of sperm IZUMO1 promotes sperm–egg fusion in mice

(2015) Naokazu Inoue et al.   Nature Communications

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization

(2014) M. Chalbi et al.   DEVELOPMENT

Juno is the egg Izumo receptor and is essential for mammalian fertilization

(2014) Enrica Bianchi et al.   NATURE

A Nonsense Mutation in TMEM95 Encoding a Nondescript Transmembrane Protein Causes Idiopathic Male Subfertility in Cattle

(2014) Hubert Pausch et al.   PLoS Genetics

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion

(2013) N. Inoue et al.   DEVELOPMENT

A transgenic insertion on mouse chromosome 17 inactivates a novel immunoglobulin superfamily gene potentially involved in sperm–egg fusion

(2013) Diego Lorenzetti et al.   MAMMALIAN GENOME

Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa

(2013) Y. Fujihara et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

(2013) Daisuke Mashiko et al.   Scientific Reports

Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1

(2012) Y. Satouh et al.   JOURNAL OF CELL SCIENCE

Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility

(2012) K. Tokuhiro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Detection of the activator cAMP responsive element modulator (CREM) isoform ortholog proteins in porcine spermatids and sperm

(2012) T. Noda et al.   THERIOGENOLOGY

Classification of Mouse Sperm Motility Patterns Using an Automated Multiclass Support Vector Machines Model1

(2011) Summer G. Goodson et al.   BIOLOGY OF REPRODUCTION

Calsperin Is a Testis-specific Chaperone Required for Sperm Fertility

(2010) Masahito Ikawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fertilization: a sperm’s journey to and interaction with the oocyte

(2010) Masahito Ikawa et al.   JOURNAL OF CLINICAL INVESTIGATION