Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 117, Issue 21, Pages 11836-11842Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1918068117
Keywords
protein-protein interaction; rice; ORFeome; next-generation sequencing; networks
Categories
Funding
- National Science Foundation (NSF) [1661380, 1639075, 1444511, 0520253, 0703905, 1237975]
- National Institute of General Medical Sciences [R01 GM124559, R01 GM125639, R01 GM122968]
- National Institute of Diabetes and Digestive and Kidney Diseases [R01 DK115398]
- National Institute of Food and Agriculture [2013-67013-21379]
- Deutsche Forschungsgemeinschaft [411255989]
- Foundation for Food and Agriculture Research [534683]
- European Research Council [648420]
- Bio-Oriented Technology Research Advancement Institution
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0520253, 1661380] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1444511, 1639075] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [0703905, 1237975] Funding Source: National Science Foundation
- European Research Council (ERC) [648420] Funding Source: European Research Council (ERC)
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Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.
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