Article
Agriculture, Dairy & Animal Science
Alexa C. B. Johnson, Amy S. Biddle
Summary: This study aimed to identify differences in reemergence rates of cyathostomin species following commercial dewormer treatment, finding that sensitivity to dewormers varies between species, indicating potential for more targeted control measures.
Article
Parasitology
Michel Boisseau, Nuria Mach, Marta Basiaga, Tetiana Kuzmina, Claire Laugier, Guillaume Salle
Summary: This study used data on equine strongyle communities and found that horse age and parasite egg excretion have a certain impact on species richness and the Gini-Simpson index. The differences between different operations are also important factors affecting species richness and dominance. In addition, within-host diversity also has a significant impact on overall diversity. Finally, analysis of necropsy data identified a network of positive co-occurrences between the four most prevalent strongyle genera.
PARASITES & VECTORS
(2023)
Article
Food Science & Technology
Yang He, Hammed Ayansola, Qihang Hou, Chaoyong Liao, Jiaqi Lei, Yujiao Lai, Qiuyu Jiang, Hori Masatoshi, Bingkun Zhang
Summary: Genistein alleviates colitis caused by Salmonella infection by regulating gut bacteria and intestinal stem cell development, inhibiting goblet cell loss.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mehrnaz Nouri, Bjorn Westrom, Shahram Lavasani
Summary: This study demonstrates signs of gastrointestinal inflammation in an experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS), and suggests calprotectin as a potential biomarker for evaluating intestinal inflammation in EAE. Increased infiltration of neutrophils and goblet cells in the intestinal tissue was found to correlate with disease activity, indicating the potential of this model for studying irritable bowel syndrome (IBS).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Beatriz Lopez-Cauce, Andres Urquia, Luis Menchen, Kohei Homma, Francisco Bolas-Fernandez, Juan J. Garcia-Rodriguez, Marta Puerto
Summary: This study evaluated the immunomodulatory effect of AHCC(R) in a murine model of Trichinella spiralis infection. The results showed that AHCC(R) could modulate the expression levels of Th1 and Th2 cytokines, reduce tissue damage, decrease the number of adult Trichinella spiralis, and promote parasite expulsion.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Developmental Biology
Nai-Xin Gu, Yu-Ru Guo, Sey-En Lin, Yen-Hsin Wang, I. -Hsuan Lin, Yi-Fan Chen, Yun Yen
Summary: Intestinal homeostasis is maintained by interactions between the intestinal epithelium, the immune system, and the microbiota. Current research suggests that genes involved in Wnt signaling play a crucial role in controlling intestinal stem cell fate and modulating intestinal homeostasis. Loss of frizzled 7 (Fzd7), an important component of the Wnt signaling pathway, disrupts the differentiation of intestinal stem cells into absorptive progenitors instead of secretory progenitors. This alteration in signaling pathways leads to epithelial homeostasis disruption and various phenotypes similar to enterocolitis, including shortened intestines, decreased goblet and Paneth cells, and severe inflammation. Loss of Fzd7 also exacerbates chemical-induced colitis and may initiate tumorigenesis.
Article
Veterinary Sciences
Ghazanfar Abbas, Mark A. Stevenson, Jenni Bauquier, Anne Beasley, Caroline Jacobson, Charles El-Hage, Edwina J. A. Wilkes, Peter Carrigan, Lucy Cudmore, John Hurley, Ian Beveridge, Martin K. Nielsen, Kristopher J. Hughes, Abdul Jabbar
Summary: This study assesses Australian veterinarians' knowledge, perceptions, and treatment strategies for worm control in horses. The findings suggest that while veterinarians have a good understanding of equine parasites, there are still some areas for improvement in terms of worm diagnosis and control, such as the frequent recommendation of prophylactic deworming and limited use of fecal egg count as a basis for deworming decisions.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Jeanette B. Black, Arthur R. Frampton
Summary: EHV-1 is a highly transmissible pathogen in horses, which can cause various clinical diseases. A major consequence is neurological disease. In this study, it was found that non-steroidal anti-inflammatory drugs and a JAK/STAT inhibitor were effective in reducing virus yields and cell-to-cell spread.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Masahiro Kitao, Ryuhei Hayashi, Kimihito Nomi, Reiko Kobayashi, Tomohiko Katayama, Hiroshi Takayanagi, Akiko Oguchi, Yasuhiro Murakawa, Kohji Nishida
Summary: The study identified BST2 as a specific marker for conjunctival epithelial stem/progenitor cells, which allowed for successful isolation and detection of these cells. BST2-positive cells exhibited high proliferative capacity and were able to generate conjunctival epithelial sheets containing goblet cells.
Article
Agriculture, Dairy & Animal Science
L. A. Melville, A. Hayward, E. R. Morgan, D. J. Shaw, D. McBean, L. Andrews, A. Morrison, F. Kenyon
Summary: The sentinel group approach, involving monitoring a small proportion of grazing lambs to determine the need for anthelmintic treatment for the whole group, was found to be effective in maintaining animal productivity and reducing monitoring requirements compared to individual-targeted treatments.
Article
Multidisciplinary Sciences
Bong-Kwang Jung, Sooji Hong, Taehee Chang, Jaeeun Cho, Seungwan Ryoo, Keon Hoon Lee, Jeonggyu Lee, Woon-Mok Sohn, Sung-Jong Hong, Virak Khieu, Rekol Huy, Jong-Yil Chai
Summary: Opisthorchis viverrini infection is highly endemic in Preah Vihear and Stung Treng provinces, Cambodia, with a low-grade mixed infection with H. taichui. The proportion of egg-positive cases for overall liver and intestinal helminths was high but not notably different between the 2 provinces.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
J. Blake Everett, Bruno C. Menarim, Sarah H. Barrett, Sophie H. Bogers, Christopher R. Byron, R. Scott Pleasant, Stephen R. Werre, Linda A. Dahlgren
Summary: This study evaluated the ability of intra-articular bone marrow mononuclear cell (BMNC) therapy to improve clinical signs of naturally occurring equine osteoarthritis (OA). The results showed significant improvements in joint circumference and objective lameness in the BMNC-treated group, with no adverse effects observed.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Plant Sciences
Marcos P. Silva, Thiago M. Silva, Ana C. Mengarda, Maria C. Salvadori, Fernanda S. Teixeira, Severino M. Alencar, Givelton C. Luz Filho, Bruno Bueno-Silva, Josue de Moraes
Summary: The study demonstrates that Brazilian red propolis has significant anthelmintic properties against Schistosoma mansoni infection, reducing worm burden and egg production in both in vitro and in vivo studies.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Respiratory System
Xuan Huang, Weijie Guan, Bin Xiang, Wei Wang, Yanqing Xie, Jinping Zheng
Summary: This study demonstrates that MUC5B plays a protective role in the development of COPD by promoting goblet cell differentiation and inhibiting inflammation. The regulation of inflammation by MUC5B is related to macrophage function, while goblet cell differentiation is promoted through the induced expression of STAT6 and SPDEF.
RESPIRATORY RESEARCH
(2022)
Article
Physiology
Virinchi N. S. Kuchibhotla, Malcolm R. Starkey, Andrew T. Reid, Irene H. Heijink, Martijn C. Nawijn, Philip M. Hansbro, Darryl A. Knight
Summary: Excessive mucus production is a major feature of allergic asthma, and the disruption of epithelial junctions by allergens like house dust mite activates the beta-catenin signaling pathway, promoting goblet cell differentiation. The study specifically focused on the role of beta-catenin/CBP signaling pathway in goblet cell metaplasia in a mouse model of HDM-induced allergic airway disease, and found that inhibition of this pathway with ICG-001 attenuated goblet cell metaplasia and macrophage infiltration.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Parasitology
Ashley E. Steuer, Haley P. Anderson, Taylor Shepherd, Morgan Clark, Jessica A. Scare, Holli S. Gravatte, Martin K. Nielsen
Summary: This study investigates the patterns of strongylid egg shedding and transmission dynamics in naturally infected and untreated mares and foals in Kentucky, USA. The study finds that there is no seasonality in strongylid egg shedding among the mature horses throughout the year and no peri-parturient rise in egg counts. The study also reveals that the gender and age of foals have an impact on the egg shedding.
PARASITES & VECTORS
(2022)
Article
Parasitology
Martin K. Nielsen, Georg von Samson-Himmelstjerna, Tetiana A. Kuzmina, Deborah C. K. van Doorn, Aranzazu Meana, Steffen Rehbein, Timothy Elliott, Craig R. Reinemeyer
Summary: This document provides guidelines for the design, execution, and interpretation of studies evaluating the efficacy of anthelmintic drugs against internal parasites in equines. It outlines the design and execution of critical and controlled studies, discussing their advantages and disadvantages. The document includes specific considerations for different target parasites, as well as information on animal selection, randomization procedures, housing, feeding, dosage titration, and field studies. It also provides guidance on group size determination and statistical analysis of study results. The aim of this guideline is to assist investigators in evaluating anthelmintic drugs in horses using comparable and standardized procedures with appropriate sample sizes.
VETERINARY PARASITOLOGY
(2022)
Article
Parasitology
Kinga Joo, Roxana L. Truzsi, Csenge Zs Kalman, Virag Acs, Szilard Jakab, Andras Baba, Martin K. Nielsen
Summary: Equine parasite control in Hungary relies mainly on traditional approaches without the use of regular fecal egg counts (FECs). This study highlights the importance of FEC monitoring and identifies age, anthelmintic history, and stocking density as significant risk factors associated with strongylid egg count magnitude. The findings suggest the need to investigate the efficacy of benzimidazoles in Hungary and consider reducing stocking density in cases of high strongylid FECs.
VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS
(2022)
Article
Parasitology
Martin K. Nielsen, Brooke A. Littman, Sierra W. Orzech, Nichol E. Ripley
Summary: This study aimed to evaluate the efficacy of ivermectin in controlling equine cyathostomins, a parasitic worm commonly found in grazing horses. The study found evidence of ivermectin resistance in one group of yearlings, while the remaining groups showed no resistance. The study also analyzed the factors influencing strongylid egg shedding levels in mares.
PARASITOLOGY RESEARCH
(2022)
Article
Parasitology
Michael P. Reichel, Andrew W. Greer, Martin K. Nielsen, Theo de Waal
VETERINARY PARASITOLOGY
(2022)
Article
Parasitology
Thomas Geurden, Emily R. Smith, Jozef Vercruysse, Tom Yazwinski, Terry Settje, Martin K. Nielsen
Summary: The general guidelines on anthelmintic efficacy by WAAVP provide assistance to researchers in planning, conducting, and interpreting studies on the effectiveness of anthelmintic drugs. These guidelines cover dosage determination, dosage confirmation, and field studies applicable to all animal host species, and are supplemented by species-specific guidelines for more specific guidance.
VETERINARY PARASITOLOGY
(2022)
Article
Immunology
Ashley E. Steuer, Kirsten Scoggin, John C. Stewart, Virginia D. Barker, Amanda A. Adams, Alan T. Loynachan, Martin K. Nielsen
Summary: This study aimed to investigate the effects of anthelmintic treatment with moxidectin and ivermectin on local and systemic inflammatory responses, as well as goblet cell-associated components, in horses infected with cyathostomin parasites. The results showed that removal of the parasites reduced the pro-inflammatory response, and moxidectin treatment resulted in the lowest inflammatory reaction. The different regions of the colon responded differently to the cyathostomin larvae.
PARASITE IMMUNOLOGY
(2022)
Article
Parasitology
Matthew J. Denwood, Ray M. Kaplan, Iain J. McKendrick, Stig M. Thamsborg, Martin K. Nielsen, Bruno Levecke
Summary: The faecal egg count reduction test (FECRT) is a crucial tool for detecting anthelmintic resistance in livestock. This study provides a comprehensive overview of the quantitative issues involved in FECRT and introduces a new approach for determining sample size requirements. The study also presents a classification system for anthelminthic drug efficacy based on a solid statistical framework. The findings of this study will contribute to the upcoming FECRT WAAVP guidelines and are available as open-source software.
VETERINARY PARASITOLOGY
(2023)
Article
Parasitology
Abdul Ghafar, Ghazanfar Abbas, Anne Beasley, Jenni Bauquier, Edwina J. A. Wilkes, Caroline Jacobson, Emma McConnell, Charles El-Hage, Peter Carrigan, Lucy Cudmore, Brett Tennent-Brown, John Hurley, Martin K. Nielsen, Charles G. Gauci, Ian Beveridge, Kristopher J. Hughes, Abdul Jabbar
Summary: This systematic review evaluates molecular investigations on the diagnosis and quantification of gastrointestinal helminths (GIH) in equids, identifies knowledge gaps, and discusses future research directions in equine parasitology. PCR was employed in all of the studies, with sequencing of PCR amplicons in 18 of them. Other techniques used include reverse line blot, qPCR, restriction fragment length polymorphism, nestedPCR, PCR-directed next-generation sequencing, and various other methods. The majority of studies targeted nuclear ribosomal RNA, with only a few studies targeting other loci. The review highlights the importance of advanced metabarcoding techniques for surveillance of parasite populations and the need for further research in this field.
VETERINARY PARASITOLOGY
(2023)
Article
Parasitology
Ray M. Kaplan, Matthew J. Denwood, Martin K. Nielsen, Stig M. Thamsborg, Paul R. Torgerson, John S. Gilleard, Robert J. Dobson, Jozef Vercruysse, Bruno Levecke
Summary: This paper presents guidelines for improving the standardization and performance of the FECRT. The guidelines cover experimental design, choice of FEC method, statistical analysis, and interpretation of results. Specific guidance is provided for different livestock species, and the guidelines adapt to different anthelmintic drugs and parasite species.
VETERINARY PARASITOLOGY
(2023)
Article
Parasitology
Stephanie L. Macdonald, Ghazanfar Abbas, Abdul Ghafar, Charles G. Gauci, Jenni Bauquier, Charles El-Hage, Brett Tennent-Brown, Edwina J. A. Wilkes, Anne Beasley, Caroline Jacobson, Lucy Cudmore, Peter Carrigan, John Hurley, Ian Beveridge, Kristopher J. Hughes, Martin K. Nielsen, Abdul Jabbar
Summary: This paper reviews the available data on the egg reappearance period (ERP) of cyathostomins against the three major classes of anthelmintics. A total of 54 studies met the inclusion criteria for the systematic review. Until 2022, there was no agreed definition of the ERP, and different definitions in the literature complicated the comparison between studies. Future research is needed to understand the mechanism(s) behind the shortening of ERP for cyathostomins.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2023)
Article
Microbiology
Babafela Awosile, Chiquito Crasto, Md. Kaisar Rahman, Ian Daniel, SaraBeth Boggan, Ashley Steuer, Jason Fritzler
Summary: The fecal microbiota of coyotes are dominated by Bacteroidetes, Firmicutes, and Proteobacteria, while the fecal microbiota of wild hogs are dominated by Bacteroidetes, Spirochaetes, Firmicutes, and Proteobacteria. Several human-related diseases were statistically associated with the fecal microbiota of both species.
Article
Parasitology
William B. Stoughton, Samantha Begin, Susan Outman, Henrik Stryhn, Jenny Yu, Gary Conboy, Martin K. Nielsen
Summary: The widespread overuse of anthelmintics has resulted in the development of intestinal parasites resistant to control measures in horses. A paradigm shift in equine parasite control is necessary to prevent further resistance development and maintain equine health. This study aims to investigate the egg shedding patterns and parasite management practices in Prince Edward Island (PEI), Canada, in order to develop region-specific recommendations.
VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS
(2023)
Article
Parasitology
Martin K. Nielsen, Dave M. Leathwick, Christian W. Sauermann
Summary: In the past three decades, the egg reappearance periods (ERPs) for macrocyclic lactone anthelmintics in horses have become significantly shorter. This study used computer simulations to evaluate the impact of shortened ERPs on the efficacy of ivermectin and moxidectin against equine cyathostomins. The results showed a substantial loss in anthelmintic performance due to the shortened ERPs.
VETERINARY PARASITOLOGY
(2023)
Article
Parasitology
M. K. Nielsen
Summary: This study reports fecal egg count data from a Thoroughbred operation in Central Kentucky in 2023, showing low efficacy of anthelmintic drugs against Anoplocephalid tapeworms in horses. The findings raise concerns about the possible development of anthelmintic resistance and highlight the need for developing alternative treatment options and refining evaluation methods for anti-cestodal treatment efficacy.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2023)