Journal
NATURE METHODS
Volume 17, Issue 7, Pages 681-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-020-0847-3
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Funding
- Max Planck Society
- ANR-DFG project FEMTO-ASR [ANR-14-CE35-0015-01]
- Labex NIE [ANR-11-LABX-0058_NIE]
- Agence Nationale de la Recherche (ANR) [ANR-14-CE35-0015] Funding Source: Agence Nationale de la Recherche (ANR)
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Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering. Theoretical and experimental guidelines to determine laser intensity for single-photon excitation in microcrystals for ultrafast pump-probe experiments by serial femtosecond crystallography.
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