Neisseria gonorrhoeae-induced salpingitis is targeted by circular RNA EIF3K via miR-139-5p and regulating MAPK/NF-κB signaling pathway to promotes apoptosis and autophagy bacterial cells

Background: Salpingitis is a part of pelvic inflammation, which is a threat for women's health. With development of genetic engineering, circular RNAs were discovered to have connections with diseases of human. Methods: RT-qPCR was for analyzing expressions of EIF3K, miR-139-5p and RNAs related to apoptosis. CCK-8 was applied for detecting cell viabilities. Expressions of proteins in autophagy and MAPK/NF-kappa B signaling pathway was detected by western blot. Luciferase report assay was used to make sure binding situation between circEIF3K and miR-139-5p in cells. Results: Circular EIF3K expressed lower in inflammatory cells with suppressing cell viabilities and promoting apoptosis and autophagy. MiR-139-5p could bind circEIF3K and promoted cell viabilities. Meanwhile, miR-139-5p could suppress apoptosis and autophagy. Functions of overexpressed EIF3K were restored by miR-139-5p in cell viabilities, apoptosis and autophagy. Proteins in MAPK/NF-kappa B signaling pathway expressed higher in inflammatory cells and overexpressed EIF3K could down regulate expressions of proteins. Inhibition then was resumed by up-regulated miR-139-5p. After proteins in MAPK signaling pathway was activated, apoptosis and autophagy was suppressed. Conclusion: Circular EIF3K suppressed cell growth in salpingitis through inhibiting MAPK/NK-kappa B signaling pathway, which could be a potential factor for further studies in slpingitis.