Article
Food Science & Technology
Daria Melnikova, Reindert Nijland, Timur Yu Magarlamov
Summary: The complete genome of the TTX-producing bacterium Bacillus sp. 1839 has been reported and classified as Cytobacillus gottheilii, providing valuable insights for studying the biosynthetic route of TTX.
Article
Food Science & Technology
Anna E. Vlasenko, Vasiliy G. Kuznetsov, Grigorii Malykin, Alexandra O. Pereverzeva, Peter V. Velansky, Konstantin Yakovlev, Timur Yu Magarlamov
Summary: The study investigated the accumulation and usage of toxins in nemertean worms. It found that TTX-positive cells released secretions actively in response to external stimuli, and revealed low probability of targeted TTX usage as a repellent. Additionally, mutations in Nav1 channels were identified, potentially contributing to nemertean channel resistance to TTX.
Review
Food Science & Technology
Daria I. Melnikova, Timur Yu. Magarlamov
Summary: This review summarizes the current knowledge on the circulation of TTX inside TTX-bearing animal bodies, aiming to address the gaps in understanding the biological function of TTX and facilitate further research involving TTX-bearing animals.
Article
Biotechnology & Applied Microbiology
Grigorii V. Malykin, Peter V. Velansky, Daria I. Melnikova, Timur Yu. Magarlamov
Summary: The study investigates the presence and localization of tetrodotoxin (TTX) in the larvae of the toxic ribbon worm, Cephalothrix cf. simula, through various developmental stages. Results showed that TTX was found in all cells of newly hatched larvae and accumulated in specific structures and tissues, such as the ectodermal structures and mesoderm. The level and relative proportion of TTXs remained consistent throughout development, indicating that the larvae obtained the toxins from maternal eggs and retained them.
MARINE BIOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Grigorii Malykin, Alexei Chernyshev, Timur Yu Magarlamov
Summary: The study investigated the distribution of TTX in the ribbon worms of the genus Cephalothrix, revealing that the toxin mainly accumulates in various important organs and tissues and migrates from the digestive system to target organs through blood vessels, ultimately being excreted from the body through the nephridia and mucus of epidermal cells.
Article
Food Science & Technology
Anna E. Vlasenko, Timur Yu. Magarlamov
Summary: This study investigated the variations in Tetrodotoxin (a neurotoxin) and its analogues in two marine species from the Sea of Japan. The toxin concentrations varied greatly within each species, providing insights into the migration pathways of TTXs in ecosystems.
Review
Infectious Diseases
Chien-Hao Tseng, Chia-Wei Liu, Po-Yu Liu
Summary: Animals have been identified as potential reservoirs and vectors of resistance genes, with studies showing that Gram-negative bacteria can acquire resistance through the horizontal transmission of resistance genes on plasmids. Our objective is to compile all ESBL-producing bacteria isolated from various animals in recent years and provide a comprehensive viewpoint. ESBL-producing bacteria are present in animals from various countries around the world, with farm animals being the most common sources and Escherichia coli and Klebsiella pneumoniae being the most frequently isolated bacteria.
Article
Spectroscopy
Lijun Yin, Min Fan, Qiutian She, Ruiyun You, Yudong Lu, Dechan Lu, Minlin Li
Summary: A novel dual-molecule calibration aptasensor based on Surface-enhanced Raman scattering (SERS) was developed for highly sensitive and specific detection of Tetrodotoxin (TTX). The aptasensor exhibited a wide linear range, low detection limit, and practical significance for TTX analysis in real seafood samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biotechnology & Applied Microbiology
Hikaru Oyama, Masaaki Ito, Rei Suo, Naoko Goto-Inoue, Mizuki Morisasa, Tsukasa Mori, Haruo Sugita, Tetsushi Mori, Ryota Nakahigashi, Masaatsu Adachi, Toshio Nishikawa, Shiro Itoi
Summary: In the toxic flatworm Planocera multitentaculata, the localization of tetrodotoxin (TTX) and its analogues varies during different stages of maturity, with 5,6,11-trideoxyTTX being abundant during spawning and 11-norTTX-6(S)-ol consistently localized in the pharyngeal tissue.
MARINE BIOTECHNOLOGY
(2022)
Review
Food Science & Technology
Gary M. Bucciarelli, Maren Lechner, Audrey Fontes, Lee B. Kats, Heather L. Eisthen, H. Bradley Shaffer
Summary: TTX is a potent neurotoxin that can be therapeutic at levels below lethal doses, showing promise in treating cancer-related pain, neuropathic pain, and other ailments. Despite its lethal effects on humans, TTX has potential to treat various medical conditions.
Article
Chemistry, Medicinal
Panagiota Katikou, Cengiz Gokbulut, Ali Riza Kosker, Monica Campas, Fatih Ozogul
Summary: Tetrodotoxin is a potent marine toxin found in various marine and terrestrial organisms. Its toxic effects and the lack of an antidote make TTX poisoning a significant global health concern.
Review
Food Science & Technology
Rafael Gonzalez-Cano, M. Carmen Ruiz-Cantero, Miriam Santos-Caballero, Carlos Gomez-Navas, Miguel A. Tejada, Francisco R. Nieto
Summary: Tetrodotoxin (TTX) is a potent neurotoxin that blocks voltage-gated sodium channels, exhibiting promising analgesic effects in treating neuropathic pain. However, it can also produce major toxic effects at therapeutic doses, necessitating careful consideration for clinical use. Research indicates potential therapeutic effects of other natural toxins that selectively block TTX-sensitive VGSCs for alleviating neuropathic pain.
Article
Environmental Sciences
Hanyuan Zhang, Peizhen Li, Biyin Wu, Jilun Hou, Jiangong Ren, Youxiu Zhu, Jian Xu, Fei Si, Zhaohui Sun, Xia Liu
Summary: This study identified functional genes related to TTX metabolism in the blood, liver, and muscle of tiger pufferfish. These genes include antioxidant enzymes, membrane transporters, and anti-apoptotic factors. Particularly, a large number of SLC genes were found to play critical roles in TTX accumulation and translocation in the pufferfish liver.
Article
Pharmacology & Pharmacy
Toms C. Joseph, D. B. Goswami, M. A. Pradeep, T. K. Anupama, Ejaz Parmar, V. Renuka, S. Remya, C. N. Ravishankar
Summary: A case of puffer fish poisoning was reported in the coastal city of Veraval, Gujarat state, India, with symptoms including giddiness, vertigo, aphasia, and heaviness of head following consumption of cooked fish. The patient was treated with symptomatic and supportive care, and was discharged in stable condition after 4 days. The suspected fish species, Arothron stellatus, was later identified through DNA sequencing with 100% match.
Article
Biotechnology & Applied Microbiology
Xue He, Hexing Wu, Yaping Ye, Xiaolin Gong, Baolong Bao
Summary: This study investigated the effects of exogenous TTX treatment on Takifugu flavidus. The results demonstrated that exogenous TTX administration significantly increased the level of TTX concentration in various organs. Transcriptome sequencing and analysis revealed differential expression profiles of mRNA and piRNA after TTX administration. The study also found significant apoptosis in the testis of the TTX-treated group. Overall, the response of the ovary and testis to TTX administration was different.
Letter
Evolutionary Biology
Malin Strand, Jon Norenburg, Jose E. Alfaya, Fernando Angel Fernandez-Alvarez, Hakan S. Andersson, Sonia C. S. Andrade, Thomas Bartolomaeus, Patrick Beckers, Gregorio Bigatti, Irina Cherneva, Alexey Chernyshev, Brian M. Chung, Joern von Doehren, Gonzalo Giribet, Jaime Gonzalez-Cueto, Alfonso Herrera-Bachiller, Terra Hiebert, Natsumi Hookabe, Juan Junoy, Hiroshi Kajihara, Daria Kraemer, Sebastian Kvist, Timur Yu Magarlamov, Svetlana Maslakova, Cecili B. Mendes, Robert Okazaki, Christina Sagorny, Megan Schwartz, Shi-Chun Sun, Per Sundberg, James M. Turbeville, Cong-Mei Xu
Article
Zoology
Timur Yu Magarlamov, Vyacheslav Dyachuk, Alexey V. Chernyshev
FRONTIERS IN ZOOLOGY
(2020)
Article
Chemistry, Medicinal
Daria I. Melnikova, Anna E. Vlasenko, Timur Yu. Magarlamov
Article
Pharmacology & Pharmacy
A. E. Vlasenko, V. G. Kuznetsov, I. Yu Petrova, T. Yu Magarlamov
Article
Food Science & Technology
Anna E. Vlasenko, Timur Yu Magarlamov
Article
Multidisciplinary Sciences
Timur Yu Magarlamov, James M. Turbeville, Alexei Chernyshev
Summary: The fine structure of mature pseudocnidae of 32 species of nemerteans, representatives of 20 genera, six families, and two classes was investigated with scanning and transmission electron microscopy. Pseudocnidae are composed of four layers in most species investigated, but the degree of development and position of each layer can vary between different species. Based on the structure of the discharged pseudocnidae observed in eleven species of palaeonemerteans and in eight species of pilidiophorans, researchers distinguished three different mechanisms of core extrusion/discharge.
Article
Chemistry, Medicinal
Grigorii Malykin, Alexei Chernyshev, Timur Yu Magarlamov
Summary: The study investigated the distribution of TTX in the ribbon worms of the genus Cephalothrix, revealing that the toxin mainly accumulates in various important organs and tissues and migrates from the digestive system to target organs through blood vessels, ultimately being excreted from the body through the nephridia and mucus of epidermal cells.
Article
Food Science & Technology
Daria Melnikova, Reindert Nijland, Timur Yu Magarlamov
Summary: The complete genome of the TTX-producing bacterium Bacillus sp. 1839 has been reported and classified as Cytobacillus gottheilii, providing valuable insights for studying the biosynthetic route of TTX.
Article
Food Science & Technology
Anna E. Vlasenko, Vasiliy G. Kuznetsov, Grigorii Malykin, Alexandra O. Pereverzeva, Peter V. Velansky, Konstantin Yakovlev, Timur Yu Magarlamov
Summary: The study investigated the accumulation and usage of toxins in nemertean worms. It found that TTX-positive cells released secretions actively in response to external stimuli, and revealed low probability of targeted TTX usage as a repellent. Additionally, mutations in Nav1 channels were identified, potentially contributing to nemertean channel resistance to TTX.
Article
Food Science & Technology
Anna E. Vlasenko, Vasiliy G. Kuznetsov, Timur Yu. Magarlamov
Summary: This study provides a transcriptomic analysis of peptide toxins in 14 nemertean species, revealing that nemerteans have a much greater variety of toxins than previously thought. These findings suggest that nemerteans are a promising object for the investigation of venom diversity and evolution, as well as the search for new peptide toxins.
Article
Anatomy & Morphology
Alexandra O. Pereverzeva, Alexei Chernyshev, Timur Yu Magarlamov
Summary: This study examined the ultrastructure and histology of the epidermis and cutis of Micrura bella, a member of the Heteronemertea order. The epidermis was composed of ciliated and serous gland cells, separated from the cutis by a subepidermal extracellular matrix. The cutis contained musculature, mucous and granular gland cells, and pigment cells. The integument glands of M. bella were similar to those of the Lineus ruber species complex, with some differences.
JOURNAL OF MORPHOLOGY
(2023)
Review
Food Science & Technology
Daria I. Melnikova, Timur Yu. Magarlamov
Summary: This review summarizes the current knowledge on the circulation of TTX inside TTX-bearing animal bodies, aiming to address the gaps in understanding the biological function of TTX and facilitate further research involving TTX-bearing animals.
Article
Food Science & Technology
Anna E. Vlasenko, Timur Yu. Magarlamov
Summary: This study investigated the variations in Tetrodotoxin (a neurotoxin) and its analogues in two marine species from the Sea of Japan. The toxin concentrations varied greatly within each species, providing insights into the migration pathways of TTXs in ecosystems.
Article
Multidisciplinary Sciences
Yulia Sokolnikova, Mariia Mokrina, Timur Magarlamov, Andrey Grinchenko, Vadim Kumeiko
Summary: In this study, horse-mussel hemocytes were classified into four subpopulations based on their immune functions and morphological composition. Eosinophilic granulocytes displayed the highest immune reactivity, suggesting their importance in the immune response of Modiolus kurilensis.