Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 60, Pages 9462-9465Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc04604e
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Funding
- Swiss Nanoscience Institute (Project NanoZyme) [DPA2238]
- NCCR Molecular Systems Engineering
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Silica nanoparticles equippedwith an artificial imine reductase display remarkable activity towards cyclic imine- and NAD(+) reduction. The method, based on immobilization and protection of streptavidin on silica nanoparticles, shields the biotinylated metal cofactor against deactivation yielding over 46000 turnovers in pure samples and 4000 turnovers in crude cellular extracts.
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