Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 20, Issue 3, Pages 1409-1416Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2020.17351
Keywords
Hybridization Chain Reaction; Graphene Oxide Nanosheets; Ribonuclease H
Categories
Funding
- National Natural Science Foundation of China [21804040, 21705044, 51774128]
- Natural Science Foundation of Hunan Province of China [2019JJ50134, 2018JJ4061, 2018JJ4009, 2018JJ2672]
- key program of Hunan Provincial Department of science and technology [2016NK2096]
- Scientific Research Fund of Hunan Provincial Education Department [17A055, 18B290]
- China of Postdoctoral Science Foundation [2016T90769, 2016M592456, 2015M580707]
- Zhuzhou Key Science and Technology Program of Hunan Province (2017)
- Zhuzhou Key Science and Technology Program of Hunan Province
- Green Packaging and Security Special Research Fund of China Packaging Federation [2017ZBLY14]
Ask authors/readers for more resources
Ribonuclease H (RNase H) plays key roles in HIV virus replication process and can be used as an important target for anti-AIDS drugs screening. Therefore, we constructed a fluorescent nanosensor based on signal amplification of hybridization chain reaction (HCR) and fluorescence quenching of graphene oxide (GO) nanosheets. The nanosensor provided highly sensitive and selective platform for RNase H activity detection. This method also exhibited a good linear relationship in the range of 0.001-5 U/mL, and the detection limit was 0.0007 U/mL. Our results suggested that the developed system is a promising platform for monitoring the RNase H activity, showing great potential in the biomedical studies and drugs screening.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
