Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 9, Pages 1816-1819Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc08879h
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Funding
- China Scholarship Council
- Natural Sciences and Engineering Research Council of Canada
- University Hospital Foundation
- Canadian Institutes for Health Research
- University of Alberta
- Alberta Innovates
- Alberta Health
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By using the principle of binding-induced DNA assembly, we have developed a novel homogeneous assay that is able to convert an affinity protein binding event into a predesigned DNA assembly. The assembled DNA sequence can be ligated into an intact DNA strand and hundreds of DNA hairpins can be cleaved by a nicking endonuclease. Each cleavage releases a single-stranded DNA (ssDNA) probe that is initially caged in the DNA hairpin. This released ssDNA probe can then turn on the fluorescence signal by desorbing a fluorescently-labelled complementary DNA probe from graphene oxide through hybridization. We demonstrate that this homogeneous, isothermal, and amplifiable assay can be tailored to detect a number of proteins, including a cancer biomarker, human prostate specific antigen, at picomolar levels in both buffer and human serum samples.
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