Journal
CHEMICAL COMMUNICATIONS
Volume 52, Issue 100, Pages 14423-14425Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc08835j
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Funding
- China Scholarship Council
- Taiwanese Ministry of Education
- Universite Paris-Sud
- Agence Nationale de la Recherche [ANR-12-NANO-0007 NanoFRET]
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Compact and functional nanoparticle-antibody conjugates are of paramount importance for the development of quantum dot (QD)based immunoassays. Here, we present a simple strategy to directly conjugate IgG, F(ab')(2), and Fab antibodies via their endogenous disulfide groups directly to the inorganic ZnS shell of compact penicillamine-coated QDs. The functionality of the conjugates was demonstrated by terbium (Tb)-to-QD FRET immunoassays against prostate specific antigen in serum samples. Detection limits of 2.5 pM (0.080 ng mL(-1)) were 10 and 25 times lower compared to conjugation via maleimide-terminated ligands and polymer chains, respectively. These more compact, simple, and sensitive QD-antibody conjugates will be highly advantageous for nanocrystal-based bio-sensing applications.
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