Article
Agricultural Engineering
Megha Sailwal, Pallavi Mishra, Thallada Bhaskar, Rajesh Pandey, Debashish Ghosh
Summary: This study explores the transcriptome landscape of Rhodotorula mucilaginosa IIPL32, a red oleaginous yeast, during a two-stage fermentation process. It identifies key genes involved in fatty acid and terpenoid synthesis, with significantly higher expression in the nitrogen-limited stage. The study highlights enzymes such as diphosphomevalonate decarboxylase, acetylCoA-citrate lyase, and acetyl-CoA C-acetyltransferase as important in controlling metabolic flux, as well as acetoacetyl-CoA synthase, 3-ketoacyl-acyl carrier-protein reductase, and β-subunit enoyl reductase involved in lipid and terpenoid synthesis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chotika Gosalawit, Sumeth Imsoonthornruksa, Brandon H. Gilroyed, Lucas Mcnea, Apichat Boontawan, Mariena Ketudat-Cairns
Summary: Sixty-seven yeast strains were isolated from castor beans and strain CM33, identified as Rhodotorula paludigena, showed high lipid production potential when grown on different carbon sources. CM33 demonstrated the ability to utilize renewable waste materials for producing biolipids, indicating its value in contributing to a value-added carbon chain.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Siraprapha Siwina, Ratanaporn Leesing
Summary: This study demonstrated the conversion of durian peel into microbial lipid and biodiesel using a newly isolated yeast strain, R. mucilaginosa KKUSY14, without the need for detoxification. The lipid yield from durian peel was high, with fatty acid compositions meeting the requirements for biodiesel fuel properties.
Article
Biochemistry & Molecular Biology
Mathilde Brunel, Viktoriia Burkina, Jana Pickova, Sabine Sampels, Ali A. Moazzami
Summary: This study investigated the effects of replacing vegetable oils with oleaginous yeast biomass in the diet of Arctic char on health and composition. The results showed higher liver weight and hepatosomatic index in the experimental group fed with yeast biomass compared to the control group fed with vegetable oils. There were no significant differences in lipid content of muscle and liver tissues between the two groups.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Pariya Shaigani, Tobias Fuchs, Petra Graban, Sophia Prem, Martina Haack, Mahmoud Masri, Norbert Mehlmer, Thomas Brueck
Summary: This study successfully performed targeted gene editing in Cutaneotrichosporon oleaginosus using CRISPR/Cas system, establishing a strong foundation for sustainable microbial lipid production.
MICROBIAL CELL FACTORIES
(2023)
Article
Food Science & Technology
Chunji Li, Zhenyan Xie, Die Zhao, Ping Cheng, Guohui Yu
Summary: This study investigated the variation in lipid profiles in R. glutinis during different growth phases using lipidomic and transcriptomic technologies. The results showed that most lipids exhibited an increased yield with fermentation time, except for triacylglycerols. Down-regulation of genes involved in triacylglycerol synthesis during the stationary-late growth phases led to a decrease in triacylglycerol production. The acyl-protein thioesterase genes APT1/APT2 might play central regulatory roles in lipid metabolism across growth phases.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yang Zhang, Jie Peng, Huimin Zhao, Shuobo Shi
Summary: By constructing FAEE biosynthetic pathways, mutating the bifunctional enzyme, and employing different cultivation strategies, the production of FAEEs in Rhodotorula toruloides was successfully improved. Most of the produced FAEEs were secreted out of the cell, which could greatly reduce downstream processing costs.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Microbiology
Monica A. Fernandes, Marta N. Mota, Nuno T. Faria, Isabel Sa-Correia
Summary: This study successfully improved a Rhodotorula toruloides strain through adaptive laboratory evolution, enabling it to produce more lipids in lignocellulosic hydrolysate medium containing inhibitors. The study also found that the evolved strain exhibited changes in cell wall characteristics and cell permeability, which may contribute to its multi-tolerance to inhibitors.
Article
Agricultural Engineering
Yantao Liu, Yanan Wang, Mingliang Tang, Man Zhao, Xiaobing Yang, Lingling Li, Yi Liu, Wenting Zhou, Zhiwei Gong
Summary: A simple combination utilization of water hyacinth hydrolysate and crude glycerol was developed to achieve lipid overproduction. The optimization of carbon-to-nitrogen ratio (C/N) and the dilution detoxification improved the quality of the lipids produced.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Microbiology
Irene Fakankun, Brian Fristensky, David B. Levin
Summary: The study conducted the first genome analysis of Rhodotorula diobovata, revealing its potential biotechnological applications as a producer of lipids and carotenoids.
Article
Engineering, Environmental
Sameh Samir Ali, Rania Al-Tohamy, Eleni Koutra, Amal H. El-Naggar, Michael Kornaros, Jianzhong Sun
Summary: The development of a novel oleaginous consortium, OYC-Y.BC.SH, using three yeast cultures has shown great potential for lipid production, textile dye/effluent removal, and lignin valorization. The consortium exhibited high lipid productivity, lipase activity, and decolorization efficiency, making it valuable for biodiesel production and bioremediation of textile effluents. Toxicological studies also support the use of OYC-Y.BC.SH for detoxification of industrial dyeing effluents.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Yuuki Kobayashi, Ayane Kayamori, Keita Aoki, Yuh Shiwa, Minenosuke Matsutani, Nobuyuki Fujita, Takashi Sugita, Wataru Iwasaki, Naoto Tanaka, Masako Takashima
Summary: Analysis of the genomes of Basidiomycota yeasts revealed differences in the relative evolutionary rates of barcode sequences, whole-genome nucleotide sequences, and chromosome synteny among fungal clades. This suggests that sequence-level distances at the barcode or whole-genome level are not sufficient for delineating species boundaries.
Article
Biotechnology & Applied Microbiology
Mingfeng Cao, Vinh G. Tran, Jiansong Qin, Andrew Olson, Shekhar Mishra, John C. Schultz, Chunshuai Huang, Dongming Xie, Huimin Zhao
Summary: This study demonstrates the metabolic engineering of Rhodotorula toruloides to achieve high production of the plant-sourced polyketide triacetic acid lactone (TAL). By introducing relevant genes and optimizing metabolic pathways, TAL production was significantly increased. The findings highlight the potential of Rhodotorula toruloides as a host for the production of TAL and other polyketides from low-cost carbon sources.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Chih-Chan Wu, Takao Ohashi, Hiroyuki Kajiura, Yu Sato, Ryo Misaki, Kohsuke Honda, Savitree Limtong, Kazuhito Fujiyama
Summary: The overexpression of RtFAD2 in oleaginous yeast Rhodotorula toruloides significantly increased the production of linoleic acid (LA), indicating a potential application in the production of LA or polyunsaturated fatty acids. This finding may assist in the rational design of metabolic engineering related to LA production in R. toruloides.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Silvia Donzella, Immacolata Serra, Andrea Fumagalli, Luisa Pellegrino, Giacomo Mosconi, Roberto Lo Scalzo, Concetta Compagno
Summary: This study demonstrates the feasibility of using oleaginous yeasts to produce lipids from wastes generated by industrial food processing. The results show that both yeasts can utilize the main sugars present in the feedstock, with R. azoricus performing the best. By conducting a two-stage process in a bioreactor, R. azoricus achieved high lipid accumulation efficiency.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Luis A. Garay, Irnayuli R. Sitepu, Tomas Cajka, Oliver Fiehn, Erin Cathcart, Russell W. Fry, Atit Kanti, Agustinus Joko Nugroho, Sarah Asih Faulina, Sira Stephanandra, J. Bruce German, Kyria L. Boundy-Mills
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2017)
Article
Microbiology
Gennadi I. Naumov, Elena S. Naumova, Kyria L. Boundy-Mills
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2018)
Review
Biotechnology & Applied Microbiology
Luis A. Garay, Irnayuli R. Sitepu, Tomas Cajka, Jian Xu, Hui Ean Teh, J. Bruce German, Zhongli Pan, Stephanie R. Dungan, David E. Block, Kyria L. Boundy-Mills
BIOTECHNOLOGY ADVANCES
(2018)
Editorial Material
Biology
Linhuan Wu, Kevin McCluskey, Philippe Desmeth, Shuangjiang Liu, Sugawara Hideaki, Ye Yin, Ohkuma Moriya, Takashi Itoh, Cha Young Kim, Jung-Sook Lee, Yuguang Zhou, Hiroko Kawasaki, Manzour Hernando Hazbon, Vincent Robert, Teun Boekhout, Nelson Lima, Lyudmila Evtushenko, Kyria Boundy-Mills, Boyke Bunk, Edward R. B. Moore, Lily Eurwilaichitr, Supawadee Ingsriswang, Heena Shah, Su Yao, Tao Jin, Jinqun Huang, Wenyu Shi, Qinglan Sun, Guomei Fan, Wei Li, Xian Li, Ipek Kurtboke, Juncai Ma
Article
Agriculture, Multidisciplinary
Kyria Boundy-Mills, Nardrapee Karuna, L. Antonio Garay, Julian M. Lopez, Calvin Yee, Alex Hitomi, Audry K. Nishi, Lauren L. Enriquez, Christopher Roberts, David E. Block, Tina Jeoh
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2019)
Review
Biotechnology & Applied Microbiology
K. Boundy-Mills, K. McCluskey, P. Elia, J. A. Glaeser, D. L. Lindner, D. R. Nobles, J. Normanly, F. M. Ochoa-Corona, J. A. Scott, T. J. Ward, K. M. Webb, K. Webster, J. E. Wertz
JOURNAL OF APPLIED MICROBIOLOGY
(2020)
Article
Entomology
Elda Vitanovic, Jeffrey R. Aldrich, Kyria Boundy-Mills, Marin Cagalj, Susan E. Ebeler, Hannah Burrack, Frank G. Zalom
JOURNAL OF ECONOMIC ENTOMOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Irnayuli Sitepu, Lauren Enriquez, Valerie Nguyen, Russell Fry, Blake Simmons, Steve Singer, Christopher Simmons, Kyria L. Boundy-Mills
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Microbiology
E. S. Naumova, K. L. Boundy-Mills, G. Naumov
Article
Biochemistry & Molecular Biology
Ryoya Tanahashi, Akira Nishimura, Minh Nguyen, Irnayuli Sitepu, Glen Fox, Kyria Boundy-Mills, Hiroshi Takagi
Summary: Proline contributes to taste and flavor of foods. Saccharomyces cerevisiae poorly assimilates proline during fermentation, resulting in its accumulation in fermented products. Our screening of 1138 yeasts revealed that Zygoascus, Galactomyces, and Magnusiomyces genera are better at utilizing proline.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Akira Nishimura, Kazuki Ichikawa, Hayate Nakazawa, Ryoya Tanahashi, Fumika Morita, Irnayuli Sitepu, Kyria Boundy-Mills, Glen Fox, Hiroshi Takagi
Summary: This study clarifies the inhibitory mechanism of proline utilization in yeast during wine-making processes, revealing the involvement of glucose response and the Ras/PKA pathway. The research indicates the existence of crosstalk between carbon and proline metabolisms and provides valuable insights and directions for developing wine yeast strains that can efficiently assimilate proline.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Mycology
Andrey Yurkov, Artur Alves, Feng-Yan Bai, Kyria Boundy-Mills, Pietro Buzzini, Neza Cadez, Gianluigi Cardinali, Serge Casaregola, Vishnu Chaturvedi, Valerie Collin, Jack W. Fell, Victoria Girard, Marizeth Groenewald, Ferry Hagen, Chris Todd Hittinger, Aleksey Kachalkin, Markus Kostrzewa, Vassili Kouvelis, Diego Libkind, Xinzhan Liu, Thomas Maier, Wieland Meyer, Gabor Peter, Marcin Piatek, Vincent Robert, Carlos A. Rosa, Jose Paulo Sampaio, Matthias Sipiczki, Marc Stadler, Takashi Sugita, Junta Sugiyama, Hiroshi Takagi, Masako Takashima, Benedetta Turchetti, Qi-Ming Wang, Teun Boekhout
Summary: The accurate application of fungal names is crucial for scientific communication, clinical diagnostics, and regulatory compliance. Maintaining the stability of the taxonomic system and ensuring the traceability of nomenclatural changes are key for a broad range of users and taxonomists. Proper preservation of nomenclatural history and physical organisms representing names is essential to assure the unambiguous application of names.