Article
Chemistry, Analytical
Zhenning Yu, Yanli Tong, Yuling Liang, Yunda Li, Hongling Yang, Si-Yang Liu, Yuzhi Xu, Zong Dai, Xiaoyong Zou
Summary: The hmC-TACN method utilizes TdT-assisted formation of fluorescent Cu nanotags to quantify global 5hmC levels with high sensitivity, requiring only a small amount of DNA input for accurate detection.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qian Yu, Hoa Duc Trinh, Yeonji Lee, Taejoon Kang, Lingxin Chen, Sangwoon Yoon, Jaebum Choo
Summary: In this study, a silica-encapsulated Au core-satellite (CS@SiO2) nanotag was developed, which exhibited strong and reproducible surface-enhanced Raman scattering (SERS) signal. By combining SERS with enzyme-linked immunosorbent assay (ELISA), a highly sensitive detection of SARS-CoV-2 was achieved. The CS@SiO2 nanotags showed superior performance with a detection limit of 8.81 PFU mL-1, which is 10 times better than conventional ELISA and 100 times better than lateral flow assay strip method. The new CS@SiO2-SERS-ELISA method can significantly reduce the false-negative diagnostic rate for SARS-CoV-2 and contribute to overcoming the current pandemic crisis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Ding Wang, Yiru Zhao, Shen Zhang, Liping Bao, Huijun Li, Jingcheng Xu, Bin He, Xumin Hou
Summary: This study developed a rapid and accurate method for the detection of acute myocardial infarction using surface-enhanced Raman scattering (SERS) technique. By preparing 4-Mercaptobenzoic acid embedded Au@Ag core-shell nanospheres, the detection of cardiac troponin I (cTn I) was successfully achieved. The prepared SERS nanotags could complete the analysis procedure within 15 minutes, indicating a faster detection speed and higher accuracy.
Article
Biophysics
Bing Liu, Shiya Zheng, Hanyu Tang, Qian Liu, Haitao Li, Bingbing Gao, Xiangwei Zhao, Fei Sun
Summary: A highly sensitive method for the analysis of free testosterone (FT) was developed using magnetic nanobeads (MNBs) and gap-enhanced surface enhanced Raman scattering (SERS) nanotags. The proposed method achieves high detection sensitivity and a wider linear dynamic detection range, making it suitable for rapid disease diagnosis and analysis of other substances.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Cunming Hu, Li Ma, Fang Mi, Ming Guan, Chang Guo, Fei Peng, Shijiao Sun, Xiaomei Wang, Tingwei Liu, Jiutong Li
Summary: A highly sensitive and rapid SERS immunoassay for cardiac troponin I (cTnI) was developed using core-shell SERS nanotags, allowing quantification of biomarkers by detecting characteristic Raman peak intensities. The method demonstrated high specificity without cross-reactivity with non-specific proteins, and when combined with magnetic beads, it enabled rapid detection without complex separation procedures. The use of a portable Raman instrument in this study highlights the potential for point-of-care testing and small sample analysis in the early diagnosis of acute myocardial infarction.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Shu Wang, Wanzhu Shen, Shuai Zheng, Zhigang Li, Chongwen Wang, Long Zhang, Yong Liu
Summary: A colorimetric-fluorescent dual-signal lateral flow assay based on Van-modified SiO2-Au-QD tags was developed for sensitive and quantitative detection of S. aureus. The method achieved visualization limits of 10(4) cells mL(-1) for colorimetric signal and 100 cells mL(-1) for fluorescence signal.
Article
Optics
Chloe Duffield, Nana Lyu, Yuling Wang
Summary: This study identified the ideal conditions for bulk synthesis of silver-gold core-shell nanoparticles with a 2:1 ratio of silver nitrate to hydroquinone, resulting in stable, abundant, and highly reproducible Raman signals. After stability testing, the SERS nanotags showed stability with minimal signal change, indicating great potential for cancer and disease diagnosis.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2021)
Article
Chemistry, Analytical
Sheng Chen, Mengya Lv, Jiayi Fan, Yanjie Huang, Gaolin Liang, Shusheng Zhang
Summary: We reported a novel bioorthogonal surface-enhanced Raman scattering (SERS) nanoprobe for accurate cancer cell imaging, which exhibited high sensitivity and ultra-accuracy. The probe, Au@4-MBN@Au-PEG-FA, was synthesized by modifying the coremolecule-shell nanoflower structure with FA-PEG-SH molecules. It demonstrated stable nitrile stretching vibration Raman signal and high selectivity for cancer cells, especially FR-positive HeLa cells. This SERS nanoprobe could be potentially applied for FR-related cancer theranostics and clinical detection in the future.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Shiliang Chen, Liuwei Meng, Litong Wang, Xixi Huang, Shujat Ali, Xiaojing Chen, Mingen Yu, Ming Yi, Limin Li, Xi Chen, Leiming Yuan, Wen Shi, Guangzao Huang
Summary: The newly developed SERS-based LFIA strip using GERTs achieved highly sensitive detection of anti-SARS-CoV-2 IgM and IgG, with detection limits 100 times lower compared to conventional LFIA strips, improving the accuracy and reliability of early diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
Ximo Wang, Chen Chen, Geoffrey I. N. Waterhouse, Xuguang Qiao, Zhixiang Xu
Summary: A DNA hydrogel surface enhanced Raman spectroscopy sensor was developed for ultrasensitive determination of streptomycin residues in foods. The sensor exhibited specificity and high sensitivity, with good recoveries in milk and honey samples.
Article
Chemistry, Analytical
Xi Zeng, Xiaoxu Guo, Shaowei Jiang, Xiaoping Yang, Zhixing Zhong, Siyu Liu, Zhi Zhu, Jia Song, Chaoyong Yang
Summary: This study presents a digital microfluidics-based single-cell reduced representation bisulfite sequencing (digital-scRRBS) technique, which is an automatic, effective, reproducible, and reagent-efficient method for dissecting the single-cell methylome. The digital-scRRBS allows for rapid isolation of single cells and construction of high-quality methylation sequencing libraries. This technology is valuable for distinguishing cell identity and tracking DNA methylation, and holds great significance for studying rare cells and populations with high levels of heterogeneity.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Pei Zeng, Yuting Zhou, Zhiwen Shu, Huikang Liang, Xiaoqing Zhang, Yiqin Chen, Huigao Duan, Mengjie Zheng
Summary: This study successfully fabricated suspended metallic nanodimers with tiny gaps, which exhibited excellent sensitivity for surface-enhanced Raman spectroscopy detection. The 3D elevated suspended metallic dimers achieved higher SERS enhancement factors compared to other types of metallic nanostructures, showing promise for high-sensitivity SERS detection and other plasmonic applications.
Article
Engineering, Electrical & Electronic
Xuan Thanh Pham, Duc Nha Duong, Ngoc Tan Nguyen, Nguyen Van Truong, Jong-Wook Lee
Summary: The proposed DSL method in CCIA achieves enhancement of Zin in the sub-Hz range, increasing it by 184 times to 4.6 G Omega at 0.01 Hz. Compared to CCIA with conventional DSL, Zin is improved by 7.8 times, while achieving an input-referred noise of 2.1 μVrms at 2.14 μW.
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS II-EXPRESS BRIEFS
(2021)
Article
Chemistry, Multidisciplinary
Hongmei Chen, Qingli Li, Qinghai Hu, Xiaodong Jiao, Wenjie Ren, Shuangshou Wang, Guosheng Peng
Summary: This research proposes a double spiral chip-embedded micro-trapezoid filter for the sensitive isolation of circulating tumor cells (CTCs) of prostate cancer by spectral detection. The filters have a high capture efficiency and the CTCs can be positively identified by hyperspectral imaging. This method shows promise for cancer diagnosis and therapy evaluation.
NANOSCALE ADVANCES
(2022)
Article
Microbiology
Qianhui Zhu, Shenghan Gao, Binghan Xiao, Zilong He, Songnian Hu
Summary: In this study, a novel plasmid prediction tool called Plasmer was proposed, which combines the advantages of shared k-mers and genomic features. Plasmer can predict plasmids in multiple species and has achieved high accuracy on contigs above 500 bp. It outperforms existing methods in terms of performance and has balanced sensitivity and specificity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Multidisciplinary
Jing Wang, Yung-Ching Kao, Quan Zhou, Alain Wuethrich, Mitchell S. Stark, Helmut Schaider, H. Peter Soyer, Lynlee L. Lin, Matt Trau
Summary: Sensitive nanotechnology is used to profile serum extracellular vesicles for early melanoma detection, and the combination of microfluidic device and SERS technology can accurately differentiate melanoma patients from healthy individuals.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Caizhi Liao, Fiach Antaw, Alain Wuethrich, Matt Trau
Summary: A T-junction-shaped dual pore system is proposed to improve the accuracy and measurement range of detecting nanoparticles, and it has been successfully applied in characterizing the protein corona of bioparticles.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Biology
Bingyin Peng, Naga Chandra Bandari, Zeyu Lu, Christopher B. Howard, Colin Scott, Matt Trau, Geoff Dumsday, Claudia E. Vickers
Summary: This study addresses the problem of temporal control in microbial metabolic engineering by using a modular engineering approach. New genetic circuits were designed and constructed to achieve precise control of heterologous pathways driven by the GAL promoter, resulting in increased production efficiency.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Bingyin Peng, Lygie Esquirol, Zeyu Lu, Qianyi Shen, Li Chen Cheah, Christopher B. Howard, Colin Scott, Matt Trau, Geoff Dumsday, Claudia E. Vickers
Summary: Insufficient gene expression levels causing bottlenecks in metabolic pathways are a problem in industrial bioproduction. Current approaches to increase gene dosage have limitations. This study presents HapAmp, a method that uses haploinsufficiency to drive gene amplification in vivo. HapAmp allows efficient integration of heterologous gene copies, leading to significant improvements in metabolic engineering and protein production in yeast.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Cathryn M. Kolka, Julie Webster, Ailin Lepletier, Clay Winterford, Ian Brown, Renee S. Richards, Wioleta M. Zelek, Yilang Cao, Ramlah Khamis, Karthik B. Shanmugasundaram, Alain Wuethrich, Matt Trau, Sandra Brosda, Andrew Barbour, Alok K. Shah, Guy D. Eslick, Nicholas J. Clemons, B. Paul Morgan, Michelle M. Hill
Summary: This study confirms the increased formation of C5b-9 in esophageal adenocarcinoma (EAC) and demonstrates the capability and heterogeneity in C5b-9 formation and shedding in Barrett's Esophagus (BE) and EAC cell lines following sublytic C9 exposure.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Kristina Rhee, Anastasiia Tukova, Mohammad Tavakkoli Yaraki, Yuling Wang
Summary: This paper presents a simple synthesis method to produce quasi-spherical SERS nanotags and larger highly anisotropic nanoparticles, which contain gold/silver nanostars, a self-assembled monolayer of Raman reporter molecules, and a silver coating. The Raman reporter molecule, 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), is found to play a crucial role in controlling the morphology of the nanosupernova.
Article
Chemistry, Multidisciplinary
Junrong Li, Abu A. Sina, Fiach Antaw, David Fielding, Andreas Moller, Richard Lobb, Alain Wuethrich, Matt Trau
Summary: This study investigates the use of small extracellular vesicles (sEVs) molecular profiles in differentiating malignant and benign lung nodules via a liquid biopsy-based approach. The researchers develop a novel chip called DECODE that can analyze sEV biomarkers expression and generate digitally acquired sEV molecular profiles. The results show that DECODE can successfully separate malignant from benign lung nodules, suggesting its potential for non-invasive characterization of lung nodules found in lung cancer screening.
Article
Chemistry, Physical
Nhung Thi Tuyet Nguyen, Mohammad Tavakkoli Yaraki, Yuling Wang
Summary: Surface-enhanced Raman scattering (SERS) is a non-invasive analytical technique with ultra-sensitivity and high multiplex capability. Gold nanostars (AuNSs) with sharp tips and corners, and a unique near-infrared localized surface plasmon resonance, have shown great potential in SERS and biomedical applications. In this study, we report the reproducible synthesis of AuNSs using a seedless, surfactant-free, and green method. The AuNSs' localized surface plasmon resonance (LSPR) was tuned, and seven types of SERS nanotags were prepared for spectral barcoding, resulting in 127 barcodes. This spectral barcoding approach opens a new avenue for future multiplex applications.
MOLECULAR SYSTEMS DESIGN & ENGINEERING
(2023)
Article
Chemistry, Physical
Liwen Yuan, Yanpin Chen, Longfeng Ke, Quan Zhou, Jiayou Chen, Min Fan, Alain Wuethrich, Matt Trau, Jing Wang
Summary: Developing a minimally invasive technique for early-stage lung cancer detection is crucial. However, detecting rare tumor-associated extracellular vesicles (EVs) in blood remains challenging. In this study, we integrated gold core-silver shell nanoparticles and nanoscopic mixing in a microfluidic assay to accurately analyze EVs in plasma without pre-isolation. Our nanostrategy achieved high sensitivity and specificity in differentiating early-stage lung cancer from benign lung diseases, showing great potential for detecting the early occurrence of lung cancer.
NANOSCALE HORIZONS
(2023)
Article
Chemistry, Multidisciplinary
Anastasiia Tukova, Yihan Nie, Mohammad Tavakkoli Yaraki, Ngoc Thanh Tran, Jiaqiu Wang, Alison Rodger, Yuantong Gu, Yuling Wang
Summary: Gold nanoparticles (AuNPs) undergo changes upon contact with biological media, leading to the formation of protein corona (PC) around the particles. Understanding the mechanisms and functions of PC formation is important for utilizing its benefits and avoiding drawbacks. This study investigates the differences in PC formation on spherical and anisotropic AuNPs, revealing that protein does not fully cover the surface of anisotropic nanoparticles, providing potential binding sites for analytes while stabilizing the AuNPs.
Review
Chemistry, Multidisciplinary
Mohammad Tavakkoli Yaraki, Anastasiia Tukova, Yuling Wang
Summary: This review discusses the application of surface-enhanced Raman scattering (SERS) in the study of cells and extracellular vesicles (EVs). By utilizing the electromagnetic and chemical mechanisms, SERS can overcome the limitations in current research and become a non-invasive ultrasensitive tool. The review summarizes the recent advances in designing SERS biosensors using nanomaterials and label-aided or label-free approaches, and explores the potential of SERS in future biomedical applications.
Article
Chemistry, Analytical
Wei Zhang, Ling Wang, Dan Li, Douglas H. Campbell, Bradley J. Walsh, Nicolle H. Packer, Qing Dong, Erkang Wang, Yuling Wang
Summary: Circulating sEVs derived from PDAC cells have potential applications in cancer diagnosis. The phenotype of sEVs is closely associated with the presence and progression of PDAC, and can be validated through various imaging techniques. The proposed SERS assay enables simultaneous detection of multiple surface membrane proteins on sEVs, providing a potential method for PDAC diagnosis and early cancer stage prediction.
ANALYTICAL METHODS
(2022)
Review
Genetics & Heredity
Nicolas Constantin, Abu Ali Ibn Sina, Darren Korbie, Matt Trau
Summary: Liquid biopsy testing has emerged as a promising approach for screening multiple cancers simultaneously, offering the potential to increase early cancer detection rates and improve population health. Utilizing circulating tumor DNA analysis of methylation biomarkers could enhance the accuracy of cancer detection and provide valuable information for clinical decision making and patient care improvement.
Article
Chemistry, Multidisciplinary
Kevin M. Koo, Matt Trau
Summary: This study describes an accurate method for analyzing gene fusion mutant variants in patient liquid biopsy samples. The method involves the enrichment of multiple mutant variants using locker probes and the use of magneto-bioelectrocatalytic cycling readout for signal enhancement. This approach shows potential for non-invasive liquid biopsies in cancer screening, diagnostics, and therapeutics.
Review
Materials Science, Multidisciplinary
Karthik Balaji Shanmugasundaram, Junrong Li, Abu Ibn Sina, Alain Wuethrich, Matt Trau
Summary: Liquid biopsy in precision oncology offers dynamic assessment of tumor heterogeneity, minimally invasive procedures, and cost-effective tests. Microfluidic techniques integrated with surface enhanced Raman scattering (SERS) provide a powerful strategy for simultaneous detection of multiple cancer biomarkers in liquid biopsy, supporting precision oncology.
MATERIALS ADVANCES
(2022)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
