Article
Environmental Sciences
Yibei Cheng, Yimin Li, Wenru Li, Yuanyuan Song, Rensen Zeng, Kai Lu
Summary: The study demonstrates that inhibition of hepatocyte nuclear factor 4 confers imidacloprid resistance in brown planthopper by regulating cytochrome P450 and UDP-glycosyltransferase genes. Knockdown of HNF4 enhances resistance to imidacloprid in BPH, leading to up-regulation of cytochrome P450- and UGT-mediated metabolic detoxification pathways. Over-expression of UGT-1-7, UGT-2B10, and CYP6ER1 in the resistant strain indicates potential targets for pest management.
Article
Biochemistry & Molecular Biology
Kai Lu, Yimin Li, Yibei Cheng, Wenru Li, Yuanyuan Song, Rensen Zeng, Zhongxiang Sun
Summary: Increased production of detoxification enzymes is a primary mechanism for insecticide resistance in crop pests. This study identified the NR2E nuclear receptor HR83 as critical for chlorpyrifos resistance in brown planthoppers, regulating the expression of detoxification genes. Knockdown of HR83 led to decreased resistance to chlorpyrifos and reduced expression of multiple detoxification genes, essential for the resistance of BPH to chlorpyrifos. This research suggests that regulation of detoxification genes may be an ancestral function of the NR2E nuclear receptor subfamily.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shuai Wu, Minrong He, Fujin Xia, Xueyi Zhao, Xun Liao, Rongyu Li, Ming Li
Summary: This study found that the development of acetamiprid resistance in Nilaparvata lugens may be related to the overexpression of the P450 gene CYP6ER1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Yixi Zhang, Baojun Yang, Zhiming Yang, Lu Kai, Zewen Liu
Summary: Two P450 enzymes, CYP4C62 and CYP6BD12, in Nilaparvata lugens were found to bioactivate chlorpyrifos into its active ingredient chlorpyrifos-oxon in vivo and in vitro. Knockdown of these two genes reduced sensitivity to chlorpyrifos and the formation of chlorpyrifos-oxon in N. lugens. This study revealed a novel mechanism of insecticide resistance due to the reduction in bioactivation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Entomology
Tingwei Cai, Yunhua Zhang, Yu Liu, Xiaoqian Deng, Shun He, Jianhong Li, Hu Wan
Summary: This study revealed that exposure of the brown planthopper to subtoxic levels of imidacloprid altered the structure of its bacterial symbiont community, with an increase in Wolbachia abundance. These symbionts enhanced the detoxification metabolic response of the planthopper to imidacloprid, highlighting the potential risk of insecticide resistance due to changes in bacterial symbiont communities caused by subtoxic imidacloprid exposure.
Article
Agriculture, Multidisciplinary
Jinghua Zhu, Zengxin Li, Meng Zhang, Weiwei Qin, Yoonseong Park, Yueping He
Summary: This study utilized RNA-seq to identify a set of transporter and receptor genes enriched in the excretory organs of the brown planthopper (BPH). RNA interference assays showed that the knockdown of certain genes significantly affected the survival and honeydew secretion of BPH. These genes may serve as potential targets for insect control.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agronomy
Li-Xiang Wang, Sha Tao, Yan Zhang, Ya-Long Jia, Shun-Fan Wu, Cong-Fen Gao
Summary: In this study, a pymetrozine-resistant strain of brown planthopper was established, and it was found that the overexpression of NlCYP6CS1 gene is one of the key factors contributing to pymetrozine resistance. RNA interference (RNAi) suppression of NlCYP6CS1 gene expression increased the toxicity of pymetrozine against brown planthopper. These findings provide insight for proposing a management strategy for pymetrozine resistance.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Weixia Wang, Tingheng Zhu, Pinjun Wan, Qi Wei, Jiachun He, Fengxiang Lai, Qiang Fu
Summary: The study identified and functionally analyzed the SPARC gene in brown planthoppers and found that it plays an important role in their development and reproduction, mainly by regulating the tissue of the fat body.
Article
Agronomy
Yan-Chao Zhang, Yang Gao, Wen-Nan Ye, Yu-Xuan Peng, Kun Yan Zhu, Cong-Fen Gao
Summary: In this study, the CRISPR/Cas9 system was used to disrupt the P450 gene NlCYP6CS1 and its role in insecticide resistance in brown planthopper was elucidated. The knockout strain Nl6CS1-KO showed increased susceptibility to certain insecticides compared to the background resistant strain Nl-R. Life table analysis revealed differences in reproductive capacity, nymph survival rate, and body weight between Nl6CS1-KO and Nl-R. This study provides insights into the mechanism of insecticide resistance in brown planthopper and demonstrates the application of CRISPR/Cas9 system in studying similar insects.
PEST MANAGEMENT SCIENCE
(2023)
Article
Entomology
Rong-er Zheng, Jinliang Ji, Jiamin Wu, Ruijuan Zhang, Yabin Li, Xiaoping Yu, Yipeng Xu
Summary: This study evaluated the impact of the PCE3 gene on the development and reproduction of male brown planthoppers, finding that PCE3 can regulate the development of male internal genitalia and reduce oviposition levels in females mated with treated males. NlPCE3 plays an important role in reproduction in male N. lugens.
Article
Agronomy
Yan-Fei Zhou, Min Zhou, Yuan-Yuan Wang, Xin-Yi Jiang, Pei Zhang, Kang-Kang Xu, Bin Tang, Can Li
Summary: This study investigates the molecular mechanism of CYP9E2 in Tribolium castaneum in adapting to high-CO2 stress. By predicting sequence function, analyzing expression profiles, and conducting RNAi silencing experiments, the study reveals the crucial role of this protein in the beetle's tolerance to CO2 stress.
Article
Biotechnology & Applied Microbiology
Jia-Bao Lu, Ze-Dong Li, Zhuang-Xin Ye, Hai-Jian Huang, Jian-Ping Chen, Jun-Min Li, Chuan-Xi Zhang
Summary: In this study, four opsins were identified in the Nilaparvata lugens genome and one of them was found to be involved in the regulation of cuticle melanization, revealing the cross-talk between the gene pathways underlying the visual system and the morphological differentiation in insects.
Article
Biochemistry & Molecular Biology
Minrong He, Xueyi Zhao, Xingyu Chen, Yiyan Shi, Shuai Wu, Fujin Xia, Rongyu Li, Ming Li, Hu Wan, Jianhong Li, Xun Liao
Summary: The study found that Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) plays a crucial role in Nilaparvata lugens and its overexpression contributes to resistance to the insecticide sulfoxaflor as well as cross-resistance to other insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Physiology
Ruijuan Zhang, Jinliang Ji, Yabin Li, Jianbin Yu, Xiaoping Yu, Yipeng Xu
Summary: The study found that NlSLC26A10 is highly expressed in the adults of Nilaparvata lugens, particularly in the ovary. Injection of dsRNA targeting NlSLC26A10 resulted in decreased expression, hindering ovarian development and reducing the number and hatchability of eggs and yeast-like symbionts. Further investigation suggests that NlSLC26A10 may affect reproduction by reducing juvenile hormone levels and vitellogenin expression.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaojuan Jiang, Shiwen Zheng, Anfu Bamu, Huan Dai, Xinda Lin
Summary: The study cloned Nilaparvata lugens (N. lugens) ERR2 (NlERR2) and detected its expression using qRT-PCR to investigate its distribution during development and in different tissues. The interaction between NlERR2 and related genes of the 20-hydroxyecdysone (20E) and juvenile hormone (JH) signalling pathways was studied using RNAi and qRT-PCR. The findings suggest that NlERR2 is involved in hormone signalling pathways and affects moulting and ovarian development, providing a basis for new targets in pest control efforts against the brown planthopper.
INSECT MOLECULAR BIOLOGY
(2023)