Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 68, Issue 15, Pages 4367-4373Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c00029
Keywords
Triticum aestivum; arabinoxylan; nanoparticles; polyplexes; CRISPR-Cas9 delivery
Funding
- Center for Sustainable Material Science (CSMS), NDSU
- ND NSF-EPSCoR [IIA-1355466]
- USDA-NIFA-CBG [2015-38821-24344]
- NIH from the National Institute of General Medical Sciences (NIGMS) [1P20 GM109024]
Ask authors/readers for more resources
Wheat bran is a major byproduct of the wheat industry and a rich source of cellulosic and hemicellulosic compounds. We developed a facile and reproducible method to generate functional nanomaterials from wheat bran derived polysaccharide, Arabinoxylan (AX). We first established that AX derived from wheat bran was chemically equivalent to commercially available AX extracted from wheat flour. Through facile chemical modification, positive and negatively charged domains were introduced along AX backbone, which in turn induced local electrostatic and hydrophobic interactions promoting the formation of nanoparticulate structures. The extracted, chemically modified AX was characterized using FTIR, H-1 NMR, and elemental analysis. We observed that, while both anionic and cationic AX self-assemble into stable, spherical nanoparticles with a low polydispersity index, the unmodified AX did not exhibit such self-organizational properties. To form functionally active nanomaterials, we further complexed negatively charged CRISPR-Cas9 DNA with cationic AX. Through gel electrophoretic studies, we identified that, at a feed ratio of DNA to AX of 1:15, AX is capable of forming polyplexes with DNA in the form of nanoparticles with an average hydrodynamic diameter of similar to 100 nm and surface charge of -1.40 +/- 0.91 mV. We envision that chemically modified AX, originally sourced from agricultural waste materials and not from food products, can be used as functional nanomaterials for gene delivery in the agrochemical sector thus catalyzing the circular approach of sustainability.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available