Article
Biochemistry & Molecular Biology
Yunfei Tan, Lei Ma, Xiangyu Chen, Yaqin Ran, Qiulan Tong, Liwen Tang, Xudong Li
Summary: The interfacial mismatch between rigid hydroxyapatite and soft polysaccharide phase in natural polysaccharide/hydroxyapatite hydrogels remains a challenge in bone tissue engineering. In this study, hyaluronic acid was used as a template for biological mineralization to synthesize hyaluronic acid/hydroxyapatite hybrid particles. These particles were then introduced into hydroxyapatite composite hydrogels, which showed increased storage modulus and excellent cytocompatibility. The self-healing hydrogels, suitable for cell encapsulation, hold promise for bone defect repair.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Polymer Science
Lei Chen, Zhiyong Zeng, Wenbing Li
Summary: This review discusses the function and mechanism of poly(acrylic acid) (PAA) in regulating the mineralization of collagen, particularly intrafibrillar mineralization (IM). The differences between two mineralization patterns (extrafibrillar and intrafibrillar) in terms of microstructure, biodegradation, cytocompatibility, osteoinduction in vitro, and performance in vivo are systematically compared. The review also includes the mineralization of self-mineralizable collagen matrices and references regarding collagen/PAA interactions in other fields. Finally, future potential improvements in this field are discussed.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Article
Engineering, Biomedical
Jonathan H. Tsui, Andrea Leonard, Nathan D. Camp, Joseph T. Long, Zeid Y. Nawas, Rakchanok Chavanachat, Alec S. T. Smith, Jong Seob Choi, Zhipeng Dong, Eun Hyun Ahn, Alejandro Wolf-Yadlin, Charles E. Murry, Nathan J. Sniadecki, Deok-Ho Kim
Summary: Hybrid hydrogels composed of decellularized porcine myocardial extracellular matrix (dECM) and reduced graphene oxide (rGO) were developed to improve the functionality of cardiomyocytes. These hydrogels can also be used as bioinks to print cardiac tissues in a high-throughput manner. Modulating the composition and properties of the materials can enhance the electrophysiological function of cardiac tissues.
Article
Biology
Anushree Nagaraj, Alaitz Etxabide Etxeberria, Rafea Naffa, Ghada Zidan, Ali Seyfoddin
Summary: Bioprinting is an emerging technology for fabricating three-dimensional tissue constructs. Collagen, a natural protein, is used to create biocompatible scaffolds. However, its poor printability can be improved by combining it with semi-synthetic polymers. This study compares the properties of hybrid meshes made from collagen from ovine and bovine sources.
Article
Biotechnology & Applied Microbiology
Jae A. Mckee, Elisabet A. Olsen, Gideon Wills Kpeli, Moriah R. Brooks, Mohamadreza Beitollahpoor, Noshir S. Pesika, Matthew E. Burow, Mark J. Mondrinos
Summary: Physical characteristics of solid tumors, such as dense internal microarchitectures and pathological stiffness, play a role in cancer progression and treatment. This study demonstrates a method to engineer tissue constructs that mimic dense solid tumors by allowing contractile mesenchymal cells to deform and compact collagen-containing hydrogels co-seeded with carcinoma cell spheroids. These dense carcinoma constructs capture relevant physical characteristics of solid carcinomas and can be used for extended culture. This approach has the potential to provide clinically relevant venues for studying cancer pathophysiology and addressing challenges in drug delivery and cellular immunotherapy to solid tumors.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Bernette Maria Oosterlaken, Maria Paula Vena, Gijsbertus de With
Summary: Collagen mineralization is a biological process found in various skeletal elements in the animal kingdom, with a focus on understanding bone formation processes and synthesizing tissue engineering scaffolds. This paper reviews in vitro efforts towards collagen mineralization, primarily centered on hydroxyapatite and silica, with a proposed general mechanism for intrafibrillar mineralization of collagen. Future outlooks for improving collagen-based tissue engineering scaffolds are also discussed.
ADVANCED MATERIALS
(2021)
Review
Dentistry, Oral Surgery & Medicine
J. Moradian-Oldak, A. George
Summary: Biomineralization of enamel, dentin, and bone involves the deposition of apatite mineral crystals within an organic matrix. While the structure and organic composition of these tissues differ, the molecular mechanisms involving protein interactions and crystallization events are common. The review highlights the importance of protein matrix control in the mineralization process and discusses strategies for biomimetic growth of enamel and repair of demineralized dentin and bone tissue engineering.
JOURNAL OF DENTAL RESEARCH
(2021)
Review
Chemistry, Multidisciplinary
Tamara Birman, Dror Seliktar
Summary: Balancing between chemical and biological functionality is crucial to achieve injectability in hydrogels through specific crosslinking pathways, while maintaining cell compatibility. With the emergence of new injectable biosynthetic materials, their impact in cell therapy and bioprinting is becoming increasingly significant.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Fei Xu, Chloe Dawson, Makenzie Lamb, Eva Mueller, Evan Stefanek, Mohsen Akbari, Todd Hoare
Summary: This review addresses the key design criteria and emerging fabrication techniques for translationally-relevant tissue engineering hydrogels. It suggests combining integrated fabrication approaches with successful translational experiences to accelerate the implementation of hydrogels in tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Polymer Science
Xabier Morales, Ivan Cortes-Dominguez, Carlos Ortiz-de-Solorzano
Summary: This article reviews the use of hydrogel-based 3D scaffolds to study the mechanobiology of cancer cell migration. It provides an overview of the application of 3D hydrogel scaffolds in understanding the mechanical properties of cancer cell migration and how these findings relate to clinical observations. Lastly, it discusses recent methodological developments in creating realistic hydrogel-based models of the cancer ECM for studying cancer cell migration.
Article
Chemistry, Applied
Shanyi Wu, Rongjun Xiao, Yong Wu, Laijun Xu
Summary: This review discusses the emerging advances and applications of Gellan gum (GG)-based hydrogels in tissue engineering, highlighting their potential in bone, cartilage, nerve, and retina tissue engineering. The review also explores the use of GG-based hydrogels as bioink components for 3D bioprinting.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Multidisciplinary
Sarah M. Hull, Lucia G. Brunel, Sarah C. Heilshorn
Summary: Using gel-phase materials as bioinks offers advantages in providing cell protection and biological signals. Challenges remain in terms of cell compatibility and biological functionality of bioink materials.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Hsu-Hsiang Lin, Pen-Hsiu Grace Chao, Wei-Chiu Tai, Po-Chun Chang
Summary: This study successfully developed a biomimetic microfibrous system to assist PDL regeneration and withstand functional load. Collagen-based microfibers were fabricated and PDL cells seeded on waveform microfibers showed enhanced growth and gene expression levels, indicating potential for promoting healing and regeneration under shear stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Manufacturing
Frederik Wulle, Oliver Gorke, Sarah Schmidt, Maximilian Nistler, Gunter E. Tover, Oliver Riedel, Alexanser Verl, Achim Weber, Alexanser Southan
Summary: This paper introduces the potential and advantages of multi-axis 3D printing of hydrogels, and demonstrates through experiments the relative flexibility and water absorbency of 3D printed hydrogels compared to conventional molded hydrogels. The research also highlights related research questions, such as cross-linking chemistry and formulation of hydrogel inks.
ADDITIVE MANUFACTURING
(2022)
Article
Chemistry, Multidisciplinary
Gabriele Griffanti, Ehsan Rezabeigi, Jingjing Li, Monzur Murshed, Showan N. Nazhat
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Materials Science, Biomaterials
Gabriele Griffanti, Wenge Jiang, Showan N. Nazhat
BIOMATERIALS SCIENCE
(2019)
Article
Engineering, Biomedical
Gabriele Griffanti, Mark James-Bhasin, Ilaria Donelli, Giuliano Freddi, Showan N. Nazhat
BIOMEDICAL MATERIALS
(2019)