Article
Nanoscience & Nanotechnology
Rofida Albash, Menna M. Abdellatif, Mariam Hassan, Noha M. Badawi
Summary: By comparing the performance of two nanovesicles in enhancing ocular drug delivery, it was found that leciplex showed superiority over terpesomes and drug solution in corneal permeation, biofilm inhibition, and treatment of infections.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Dermatology
Shazli Razi, Samantha Ouellette, Samavia Khan, Kei Shing Oh, Thu M. Truong, Babar K. Rao
Summary: Vivascope 2500 ex vivo confocal microscopy (EVCM) is an emerging optical imaging device that allows nuclear level resolution of freshly excised tissues. It provides rapid real-time pathological examination in various subspecialties of pathology, such as skin pathology, and has advantages over traditional frozen sectioning and histological analysis.
SKIN RESEARCH AND TECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Stefania Guida, Federica Arginelli, Francesca Farnetani, Silvana Ciardo, Laura Bertoni, Marco Manfredini, Nicola Zerbinati, Caterina Longo, Giovanni Pellacani
Summary: Confocal laser scanning microscopy (CLSM) is a valuable tool in clinical settings, providing a quasi-histologic view of tissues without the need for biopsy. It has shown to improve diagnostic accuracy of skin cancers and contribute to reducing unnecessary excisions, as well as improving margin recognition for tissue sparing in excisions. Further studies using CLSM in real-life settings could enhance our understanding of its applications.
APPLIED SCIENCES-BASEL
(2021)
Article
Multidisciplinary Sciences
Hassan Shah, Asadullah Madni, Muhammad Abdur Rahim, Nasrullah Jan, Arshad Khan, Safiullah Khan, Abdul Jabar, Ahsan Ali
Summary: The study aimed to enhance solubility and permeability of diacerein by developing proliposomes and liposomal derived gel. Different formulations were investigated to optimize size, entrapment efficiency, and dissolution. Various analytical techniques were used to evaluate the physicochemical properties. In vitro and ex vivo studies demonstrated the promising performance of the liposomal gels for osteoarthritis management.
Article
Pharmacology & Pharmacy
Moaz M. El-Naggar, Mohamed A. El-Nabarawi, Mahmoud H. Teaima, Mariam Hassan, Mohammed I. A. Hamed, Ahmed A. Elrashedy, Rofida Albash
Summary: The repurposed antimicrobial impact of Levocetirizine dihydrochloride (LVC) was assessed using terpene-enriched vesicles (TPs). TPs were made using the ethanol-injection-method and their properties were characterized. The optimum TP showed sustained release of LVC and demonstrated excellent antimicrobial effect against MRSA.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Urology & Nephrology
Jesus Z. Villarreal, Javiera Perez-Anker, Susana Puig, Marc Xipell, Gerard Espinosa, Esther Barnadas, Ana B. Larque, J. Malvehy, Ricard Cervera, Arturo Pereira, Antonio Martinez-Pozo, Luis F. Quintana, Adriana Garcia-Herrera
Summary: This study demonstrates the feasibility of using ex vivo confocal microscopy for the analysis of basic patterns of lesion in nephropathology. FuCM proves to be as effective as conventional techniques in evaluating acute tubular necrosis and interstitial fibrosis changes, facilitating rapid evaluation of kidney biopsies and restructuring the clinical workflow in renal histopathology.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Polymer Science
Annu, Sanjula Baboota, Javed Ali
Summary: The study successfully optimized chitosan nanoparticles for nasal delivery to enhance the bioavailability of lurasidone hydrochloride, showing potential in improving drug release and nasal permeation.
Article
Polymer Science
Randa Mohammed Zaki, Mohamed A. Ibrahim, Doaa H. Alshora, Amal El Sayeh Abou El Ela
Summary: This study aimed to prepare Span 60-based elastic nanovesicles using the adapted ethanol injection method, and evaluate their ability to improve transdermal permeation of tacrolimus. Different concentrations and types of penetration enhancers were assessed for their impact on the physicochemical properties of the nanovesicles. The results showed significant enhancement in in vitro release compared to pure drug suspension.
Article
Oncology
Yuan Xu, Irakliy Abramov, Evgenii Belykh, Giancarlo Mignucci-Jimenez, Marian T. Park, Jennifer M. Eschbacher, Mark C. Preul
Summary: This study compares the image quality and diagnostic performance of ex vivo and in vivo CLE imaging. The results show that in vivo images have higher brightness and contrast, and better image quality in glioma, meningioma, and intracranial metastatic tumor cases. In vivo imaging also yields higher sensitivity and negative predictive value than ex vivo imaging.
FRONTIERS IN ONCOLOGY
(2022)
Article
Urology & Nephrology
Jesus Z. Villarreal, J. Perez-Anker, S. Puig, G. Pellacani, M. Sole, J. Malvehy, Luis F. Quintana, A. Garcia-Herrera
Summary: This study evaluated the feasibility of using ex vivo confocal microscopy in non-neoplastic kidney biopsies, and found that confocal images correlated well with conventional histological sections without introducing artifacts to the samples. The ease and speed of image acquisition, along with the quality of digitally stained images obtained, suggest promise for clinical applications in nephrology and renal transplantation.
JOURNAL OF NEPHROLOGY
(2021)
Article
Medicine, General & Internal
Veronika Shavlokhova, Christa Flechtenmacher, Sameena Sandhu, Michael Vollmer, Andreas Vollmer, Maximilian Pilz, Juergen Hoffmann, Oliver Ristow, Michael Engel, Christian Freudlsperger
Summary: The study demonstrates the potential of ex vivo fluorescence confocal microscopy in providing rapid real-time assessment of oral pathologies, showing high sensitivity and specificity in detecting oral leukoplakia.
Article
Pharmacology & Pharmacy
Paraskevi Papakyriakopoulou, Dimitrios M. Rekkas, Gaia Colombo, Georgia Valsami
Summary: The objective of this study is to develop and optimize a novel nasal film of Donepezil hydrochloride (DH) for the treatment of Alzheimer's disease. Factors affecting DH permeation and film flexibility were determined through experimental design, and the optimal combination was identified. The prepared nasal films exhibited good quality and stability.
Article
Cell Biology
Claudia Bernecker, Maria Augusta R. B. F. Lima, Catalin D. Ciubotaru, Peter Schlenke, Isabel Dorn, Dan Cojoc
Summary: This study introduces a novel optical tweezer approach to measure the deformation and elasticity of single red blood cells, providing new insights into the biomechanical characterization of cultured red blood cells. The results show that red blood cells cultured with human platelet lysate exhibit similar features to native red blood cells under optical forces, while those cultured with plasma display different characteristics.
Article
Polymer Science
Asif Nawaz, Arshad Farid, Muhammad Safdar, Muhammad Shahid Latif, Shakira Ghazanfar, Nosheen Akhtar, Soad K. Al Jaouni, Samy Selim, Muhammad Waseem Khan
Summary: This research developed a curcumin hydrogel containing natural essential oils as permeation enhancers and demonstrated that suitable natural permeation enhancers can improve the in vitro release and ex vivo permeation and retention of curcumin. Among various natural essential oils, 3% aloe vera oil had the greatest effect on the permeability rate and skin retention of curcumin.
Article
Biochemical Research Methods
Marc Combalia, Sergio Garcia, Josep Malvehy, Susana Puig, Alba Guembe Mulberger, James Browning, Sandra Garcet, James G. Krueger, Samantha R. Lish, Rivka Lax, Jeannie Ren, Mary Stevenson, Nicole Doudican, John A. Carucci, Manu Jain, Kevin White, Jaroslav Rakos, Daniel S. Gareau
Summary: The study developed an AI-driven XVM pathology that can generate intuitive images similar to standard histopathology and produce automated tumor positivity maps.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Polymer Science
Nabil A. Alhakamy, Usama A. Fahmy, Shaimaa M. Badr Eldin, Osama A. A. Ahmed, Hibah M. Aldawsari, Solomon Z. Okbazghi, Mohamed A. Alfaleh, Wesam H. Abdulaal, Abdulmohsin J. Alamoudi, Fatma M. Mady
Summary: This study demonstrates that incorporating quercetin, scorpion venom peptides, and Phospholipon(R) 90H into a nano-based delivery platform improves the cellular uptake and antiproliferative efficacy of quercetin, showing potential as a therapeutic approach for the treatment of breast cancer.
Article
Pharmacology & Pharmacy
Nabil A. Alhakamy, Shaimaa M. Badr-Eldin, Osama A. A. Ahmed, Hibah M. Aldawsari, Solomon Z. Okbazghi, Mohamed A. Alfaleh, Wesam H. Abdulaal, Thikryat Neamatallah, Omar D. Al-hejaili, Usama A. Fahmy
Summary: The study demonstrates that a nanoemulsion formulation of costunolide (CTD) could be a potential therapeutic approach for lung cancer treatment. The optimized CTD nanoemulsion inhibited cell proliferation and promoted apoptosis in lung cancer cells. Additionally, the formulation reduced inflammatory responses, suggesting its significance in lung cancer therapy.
Article
Pharmacology & Pharmacy
Hibah M. Aldawsari, Shaimaa M. Badr-Eldin, Nourah Y. Assiri, Nabil A. Alhakamy, Anna Privitera, Filippo Caraci, Giuseppe Caruso
Summary: This study aimed to explore the potential of surface-tailored intranasal emulsomes in enhancing the brain delivery of Vinpocetine (VNP). A factorial design was used to investigate the impact of various factors on the characteristics of emulsomes, and surface customization was performed to improve their blood-brain barrier penetration. The pharmacokinetic assessment in rats demonstrated that the surface-tailored emulsomes had higher bioavailability and brain levels compared to the oral market product, suggesting their potential as a promising platform for achieving high brain levels of VNP following intranasal administration.
Review
Pharmacology & Pharmacy
Sabna Kotta, Hibah Mubarak Aldawsari, Shaimaa M. Badr-Eldin, Anroop B. Nair, Y. T. Kamal
Summary: Polymeric micelles (PMs) have shown significant progress in drug delivery applications due to their robust core-shell structure and ability to solubilize hydrophobic drugs. This review presents the recent advances and understandings of PMs, including preparation methods, applications, and tumor-targeted delivery.
Article
Pharmacology & Pharmacy
Hani Z. Asfour, Nabil A. Alhakamy, Usama A. Fahmy, Osama A. A. Ahmed, Waleed Y. Rizg, Raed Felimban, Ashraf B. Abdel-Naim, Mohammad A. S. Abourehab, Rasha A. Mansouri, Ulfat M. Omar, Shaimaa M. Badr-Eldin
Summary: This study developed a nanosphere formula to improve the bioavailability of Icariin (ICA) using nanotechnology. By optimizing the formulation of the nanospheres, the desired particle size, zeta potential, and drug entrapment efficiency were achieved. Animal experiments showed that the optimized nanospheres significantly enhanced sexual behavior. The results suggest that ICA-tocozeinolate nanospheres have the potential to improve drug delivery and efficacy.
Review
Chemistry, Medicinal
Shaimaa M. Badr-Eldin, Hibah M. Aldawsari, Sabna Kotta, Pran Kishore Deb, Katharigatta N. Venugopala
Summary: Despite the advancements in drug discovery, it remains a tedious and expensive process. 3D cell culture models are considered promising in mimicking in vivo conditions and bridging the gap between 2D cell culture and animal models. However, there are challenges such as maintaining a micro-tissue environment and reproducibility. This review emphasizes different 3D techniques and their potential role in drug repositioning, including perspectives from the COVID-19 pandemic.
Article
Biology
Abdullah A. Alamoudi, Awaad S. Alharbi, Ashraf B. Abdel-Naim, Shaimaa M. Badr-Eldin, Zuhier A. Awan, Solomon Z. Okbazghi, Osama A. A. Ahmed, Nabil A. Alhakamy, Usama A. Fahmy, Ahmed Esmat
Summary: Diabetic hyperglycemia delays wound healing, but the ion-paired CTX-APA nanocomplex can promote wound healing in diabetic rats.
Article
Biochemistry & Molecular Biology
Shaimaa M. Badr-Eldin, Hibah M. Aldawsari, Usama A. Fahmy, Osama A. A. Ahmed, Nabil A. Alhakamy, Omar D. Al-hejaili, Alhanoof A. Alhassan, Ghadeer A. Ammari, Shouq I. Alhazmi, Raghad M. Alawadi, Rana Bakhaidar, Abdulmohsen J. Alamoudi, Thikryat Neamatallah, Singkome Tima
Summary: Ellagic acid has been studied for its potential in preventing and treating cancer. In this study, APA-functionalized emulsomes were used to enhance the cytotoxic activity of ellagic acid against breast cancer cells, resulting in significant inhibition of cell growth and induction of apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Osama A. A. Ahmed, Shaimaa M. Badr-Eldin, Giuseppe Caruso, Usama A. Fahmy, Waleed S. Alharbi, Alshaimaa M. Almehmady, Shareefa A. Alghamdi, Nabil A. Alhakamy, Amir I. Mohamed, Hibah M. Aldawsari, Fatma M. Mady
Summary: Preclinical studies have shown that statins or HMG-CoA reductase inhibitors possess anticancer activity. This study aimed to enhance the cytotoxic activity of the statin fluvastatin (FLV) by conjugating it with scorpion venom (SV) and developing colon-targeted FLV-SV nanoconjugate beads for colon cancer management. The optimized FLV-SV nanoconjugates showed improved cytotoxicity on Caco2 cells compared to individual SV and FLV, and the colon-targeted beads demonstrated efficient colon targeting ability.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Medicine, General & Internal
Nagla A. El-Shitany, Amina M. Bagher, Lenah Saeed Binmahfouz, Basma G. Eid, Haifa Almukadi, Shaimaa M. Badr-Eldin, Manal El-Hamamsy, Zuhair M. Mohammedsaleh, Fayez M. Saleh, Mohammed S. Almuhayawi, Samar A. Alghamdi, Rana A. Arab, Soad S. Ali, Steve Harakeh, Badrah S. Alghamdi
Summary: This study evaluated the short-term adverse reactions of the 2nd and 3rd doses of the Pfizer BioNTech COVID-19 vaccine. The results showed that the most common adverse reactions following the 3rd dose were injection site pain, injection site hypersensitivity, and axillary lymph node swelling. The most common systemic adverse reactions were fatigue, muscle pain, bone pain, headache, and fever less than 38°C. Women were more likely to experience vaccine adverse reactions, and the adverse reactions after the 3rd dose lasted longer than after the 2nd dose.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Mohamed A. Alfaleh, Omar Fahmy, Mohammed W. Al-Rabia, Mohammed A. S. Abourehab, Osama A. A. Ahmed, Usama A. Fahmy, Helal H. Alsulimani, Shaimaa M. Badr-Eldin, Hibah M. Aldawsari, Bander M. Aldhabi, Awaad S. Alharbi, Nabil A. Alhakamy
Summary: This study aims to investigate the combination of FLV with MEL loaded hybrid formula of PL with ALA nanoparticles to enhance anticancer tendencies. The optimized formulation showed enhanced cytotoxic potential and inhibited colon cancer cells more significantly compared to other therapeutic treatments.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Thikryat Neamatallah, Azizah M. M. Malebari, Abdulmohsin J. J. Alamoudi, Syed Nazreen, Mohammad Mahboob Alam, Hawazen H. H. Bin-Melaih, Osama A. A. Abuzinadah, Shaimaa M. M. Badr-Eldin, Gharam Alhassani, Lamar Makki, Mohammed Z. Z. Nasrullah
Summary: In this study, an optimized AG-PTMs formulation was prepared, which significantly inhibited the proliferation of liver cancer cells HepG2 through enhanced cellular uptake, cell cycle arrest and mitochondrial-dependent apoptosis.
Article
Pharmacology & Pharmacy
Hibah Mubarak Aldawsari, Sabna Kotta, Hani Z. Asfour, Sajith Vattamkandathil, Mahmoud Abdelkhalek Elfaky, Lubna Y. Ashri, Shaimaa M. Badr-Eldin
Summary: This study successfully formulated a quercetin-loaded bentonite-reinforced starch-gelatin based bioplastic with excellent mechanical properties and antimicrobial activity, which can be used for eco-friendly active food packaging and pharmaceutical applications.
SAUDI PHARMACEUTICAL JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Shaimaa M. Badr-Eldin, Hibah M. Aldawsari, Usama A. Fahmy, Osama A. A. Ahmed, Nabil A. Alhakamy, Mahmoud A. Elfaky, Alaa Sirwi, Salman A. Hawsawi, Ali H. Alzahrani, Abdulrahman Y. Yaseen, Mohannad Qassim, Sabna Kotta
Summary: Fluconazole is widely used in the treatment of cutaneous candidiasis, and a self-assembled mixed micelles topical delivery system was developed to enhance its delivery. The optimized formulation showed promising results in reducing fungal infection in immunocompromised mice, making it a potential treatment for cutaneous candidiasis.
ACTA PHARMACEUTICA
(2022)
Article
Polymer Science
Sabna Kotta, Hibah Mubarak Aldawsari, Shaimaa M. Badr-Eldin, Anroop B. Nair, Mohammed Kaleem, Mahmood Hassan Dalhat
Summary: A thermosensitive hydrogel loaded with resveratrol nanoemulsion was developed to enhance the bioavailability of resveratrol and manage breast cancer. The gel showed controlled release of resveratrol and cytotoxic effects on breast cancer cells.
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)