Article
Environmental Sciences
Supawadee Duangprom, Jirawat Saetan, Teva Phanaksri, Sineenart Songkoomkrong, Piyaporn Surinlert, Montakan Tamtin, Prasert Sobhon, Napamanee Kornthong
Summary: This study demonstrates the effectiveness of dsRNA technology in inhibiting the synthesis of VIH in Scylla olivacea females. It shows that dsRNA-VIH injections can significantly inhibit VIH expression and affect downstream genes related to ovarian maturation. Additionally, dsRNA-VIH has stimulatory effects on other reproduction-related genes, such as Scyol-PGES and Scyol-ESULT.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
ChangHong Cheng, HongLing Ma, GuangXin Liu, YiQing Deng, JianJun Jiang, Juan Feng, ZhiXun Guo
Summary: This study investigated the physiological, transcriptomic, and metabolomic responses after MCRV infection. The results showed that MCRV infection caused changes in enzyme activities, oxidative stress, and tissue damage. Transcriptome analysis identified genes related to detoxification, immune response, and metabolic processes. Two key pathways, phagocytosis and apoptosis, played important roles in response to MCRV infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yu-Kun Jie, Zhi-Ping Luo, Jia-Wei Xie, Chang-Hong Cheng, Hong-Ling Ma, Guang-Xin Liu, Jian-Jun Jiang, Yi-Qin Deng, Juan Feng, Zhi-Xun Guo
Summary: In this study, a PFK gene from the mud crab Scylla paramamosain, named SpPFK, was cloned and characterized. Knocking down SpPFK using RNA interference significantly reduced lactate dehydrogenase expression after MCDV-1 infection and increased the survival rate of mud crabs. These results suggest that SpPFK plays an important role in the proliferation of MCDV-1 in mud crabs.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Neurosciences
Krzysztof M. Wilczynski, Aleksandra Stasik, Lena Cichon, Aleksandra Augusciak-Duma, Malgorzata Janas-Kozik
Summary: Autism spectrum disorders (ASD) are a heterogeneous group of disorders that affect every population. Its pathogenesis involves the interaction of genetic and environmental factors. Social communication deficits are the main symptom of ASD, which lead to difficulties in everyday functioning.
Article
Fisheries
Yuanhao Ren, Wei Wang, Yin Fu, Zhiqiang Liu, Ming Zhao, Likun Xu, Tianyong Zhan, Ting Huang, Minghao Luo, Wei Chen, Chunyan Ma, Fengying Zhang, Keji Jiang, Lingbo Ma
Summary: This study conducted a comparative transcriptome analysis of the ovaries of mud crabs at various stages of ovarian maturation, and found that MAPK signaling pathway plays a critical role in lipid metabolism during vitellogenesis. Genes involved in carbohydrate, amino acid, and lipid metabolism were found to be highly expressed during vitellogenesis. Additionally, two genes in the PPAR signaling pathway were identified to be associated with lipid metabolism in the ovary, and MAPK signaling pathway might regulate lipid metabolism through PPAR signaling pathway based on Protein-Protein Interaction (PPI) network. These findings provide new insights into the mechanism of lipid accumulation during ovarian maturation in mud crabs.
Article
Peripheral Vascular Disease
Shubha R. R. Dutta, Purnima Singh, Kafait U. U. Malik
Summary: This study found that ovariectomy can enhance the development of Ang II-induced hypertension and renal pathophysiological changes in female mice through ALOX15 activation. This suggests that selective inhibitors of ALOX15 or 12(S)-HETE receptor antagonists could be potential treatments for hypertension and its pathogenesis in postmenopausal, hypoestrogenic women, or females with ovarian failure.
Article
Genetics & Heredity
Yuanhao Ren, Wei Wang, Zhiqiang Liu, Minghao Luo, Yin Fu, Fengying Zhang, Chunyan Ma, Ming Zhao, Wei Chen, Keji Jiang, Lingbo Ma
Summary: The study aimed to compare the differences in vitellogenesis patterns between the first and second ovarian maturation periods in the mud crab. The results showed that the ovary of the crab can re-mature after spawning, while the hepatopancreas gradually degenerates.
FRONTIERS IN GENETICS
(2022)
Article
Reproductive Biology
Ricardo Mondragon-Ceballos, Genesis Chantal Hernandez-Vazquez, Susana Rojas-Maya, Monica Dafne Garcia-Granados, Jaqueline Lugo-Ferrer, Leonor Estela Hernandez-Lopez
Summary: In stump-tailed macaques, male-female friendships promote higher post-copulatory oxytocin concentrations and better physiological conditions for males. As closeness between males and females increases, sperm concentration, immotile and motile sperm decrease gradually.
Article
Environmental Sciences
Xianliang Meng, Nishad Jayasundara, Jingyan Zhang, Xianyun Ren, Baoquan Gao, Jian Li, Ping Liu
Summary: This study investigated the toxicity and metabolism mechanisms of ammonia in the hepatopancreas of female swimming crab. Ammonia exposure resulted in reduced vtg expression, inhibition of multiple signaling pathways, and alterations in energy metabolism. Furthermore, changes in antioxidant systems, protein and lipid oxidations, and cellular apoptosis were observed, indicating metabolic suppression, oxidative stress, and apoptosis in the hepatopancreas of P. trituberculatus.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Endocrinology & Metabolism
Soledad Barez-Lopez, Agnieszka Konopacka, Stephen J. Cross, Mingkwan Greenwood, Marina Skarveli, David Murphy, Michael P. Greenwood
Summary: This study explores the expression of two genes, Caprin2 and Creb3l1, in magnocellular neurones (MCNs) and their changes during dehydration. The findings suggest that both genes are expressed in MCNs and their expression increases in response to dehydration. Protein levels mirror the increase in transcript levels for both CREB3L1 and CAPRIN2. In addition, CREB3L1 may be a transcription factor regulating Oxt gene expression, while CAPRIN2 binds to Oxt mRNA and regulates its poly(A) tail length.
NEUROENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Huixing Guo, Mangmang Wang, Xi Wang, Kang Xiao, Yangyang Huang, Haokun Hua, Wei Xiong, Wenbin Liu, Kenneth Prudence Abasubong, Wei Qiang, Cheng Chi, Guangzhen Jiang
Summary: This study evaluated the effect of cholesterol on ovarian development of Eriocheir sinensis and found that the appropriate amount of cholesterol could promote ovarian maturation by increasing the accumulation of nutrients in the ovary.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Multidisciplinary Sciences
G. Wirobski, F. S. Schaebs, F. Range, S. Marshall-Pescini, T. Deschner
Summary: The study validated a commercial Enzyme Immunoassay for measuring oxytocin metabolite concentrations in dog, wolf, and human urine samples, with improved performance compared to a previously validated assay.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Ewa Szczepanska-Sadowska, Agnieszka Wsol, Agnieszka Cudnoch-Jedrzejewska, Tymoteusz Zera
Summary: Oxytocin and vasopressin play important roles in the brain and peripheral organs, and their secretion is influenced by various factors. Inappropriate regulation may contribute to the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Zahra Niavarani, Daniel Breite, Andrea Prager, Isabell Thomas, Mathias Kuehnert, Bernd Abel, Roger Glaeser, Agnes Schulze
Summary: Composite microfiltration polyethersulfone membranes with molecularly imprinted particles (MIPs) were developed for efficient and selective adsorption of 17β-estradiol from water. The membranes exhibited significant adsorption loading and high selectivity for 17β-estradiol, even in the presence of other micropollutants. The membranes also showed excellent reusability and a maximum adsorption capacity more than 200 times higher than commercial nanofiltration.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Biochemical Research Methods
Jun-Seong Ahn, Chang-Hyun Jang
Summary: An LC-based aptasensor has been developed to detect 17 beta-estradiol (E2) at picomolar levels by utilizing competitive reactions and interfacial events. The aptasensor changes the optical response of liquid crystals (LCs) through electrostatic interactions with a cationic surfactant (CTAB) and the aptamer. The conformational change of the aptamer due to binding with E2 weakens the electrostatic attraction, resulting in a dark optical image. The E2 sensor showed a detection limit of 3.1 pM in TBS and 6.8 pM in human urine, demonstrating high sensitivity and selectivity without the need for complex analysis equipment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)