Article
Multidisciplinary Sciences
Bokun Lin, Yandan Zheng, Jingxiao Huang, Junkang Shang, Yuli Yu, Zhong Hu
Summary: An agarolytic bacterium, identified as Vibrio sp. LA1, was found on the coast of Shantou, China. The agarase gene agaA was successfully cloned and expressed in Escherichia coli, with the purified recombinant agarase showing optimal activity at 35°C and pH 6, and enhanced by the reducing reagent beta-mercaptoethanol. AgaA exhibited exo-lytic activity, producing neoagarobiose as the sole product for easier purification and production.
Article
Mathematics, Applied
Jincheng Ren, Hong-lin Liao, Jiwei Zhang, Zhimin Zhang
Summary: The traditional H-1-norm analysis leads to suboptimal error estimates for time approximations of a fractional subdiffusion problem due to the initial singularity of solution and the discrete convolution form in numerical Caputo derivatives. An improved discrete Gronwall inequality is proposed to recover theoretical accuracy in time, applied to the L1 formula and a fractional Crank-Nicolson scheme. Sharp H-1-norm error estimates are established for reaction-subdiffusion problems with nonuniform approaches through a time-space error-splitting technique and global consistency analysis. Numerical experiments confirm the sharpness of the analysis.
JOURNAL OF COMPUTATIONAL AND APPLIED MATHEMATICS
(2021)
Article
Agricultural Engineering
Na Wang, Xiaomiao Chen, Yuji Ji, Weizhi Yan, Chunmeng Chui, Li Liu, Jiping Shi
Summary: Sludge reduction is a main target for wastewater treatment, and in this study, two dominant sludge-degrading strains were found to significantly enhance sludge reduction by improving various metabolic and transport functions. This research provides new insights into the role of dominant sludge-degrading strains in reducing sludge during wastewater treatment.
BIORESOURCE TECHNOLOGY
(2021)
Article
Food Science & Technology
Kristine Majore, Inga Ciprovica
Summary: Fermentation technology is important for resource utilization and conversion of dairy waste. This study examines the conversion rate of glucose to fructose using immobilized glucose isomerase in sweet and acid whey permeates. The results highlight the potential of enzymatic hydrolysis to increase the sweetness of glucose-galactose syrup.
Article
Microbiology
Rosyada Amran Amrina, Go Furusawa, Nyok-Sean Lau
Summary: The marine bacterium CCB-QB4T is a novel rod-shaped, Gram-stain-negative, strictly aerobic organism capable of degrading alginate. It was isolated from algal turf collected off the coast of Penang, Malaysia. The strain is catalase-negative, oxidase-positive, and shows growth under a wide range of temperature, pH, and salinity conditions.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Wei Liu, Liang Ma, Liping Zhang, Yuchan Chen, Qingbo Zhang, Haibo Zhang, Weimin Zhang, Changsheng Zhang, Wenjun Zhang
Summary: Two new phenylhydrazone derivatives and one new alkaloid, along with three known compounds, were isolated from Streptomyces sp. SCSIO 40020 obtained from the Pearl River Estuary sediment. Their structures and absolute configurations were determined using NMR, mass spectroscopy and X-ray crystallography. Compound 1 showed weak cytotoxicity in three cancer cell lines. Analysis of the putative biosynthetic gene cluster suggested the involvement of a Japp-Klingemann coupling reaction in the synthesis of compounds 1 and 2.
Article
Management
Marc Goerigk, Stefan Lendl, Lasse Wulf
Summary: This study focuses on two-stage robust optimization problems as games between a decision maker and an adversary. By adding an extra adversary stage, the study extends the problem into min-max-min-max problems, advancing from two-stage settings towards more general multi-stage problems. The study specifically examines budgeted uncertainty sets and explores both continuous and discrete cases.
EUROPEAN JOURNAL OF OPERATIONAL RESEARCH
(2022)
Article
Chemistry, Applied
Maria Jose Fabra, David Talens-Perales, Adrian Roman-Sarmiento, Amparo Lopez-Rubio, Julio Polaina
Summary: Enzymatically active polysaccharide-based hydrogels and aerogels were developed using a thermostable beta-galactosidase enzyme embedded in nanoflowers. Commercial agar showed the most promising results in terms of reusability and enzymatic activity, outperforming unpurified agar and agarose. Commercial agar aerogels maintained integrity and enzyme protection, achieving similar hydrolytic activity as free enzymes and nanoflowers in hydrated form.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Organic
Yang Chen, Meng-Yao Ma, Hongguang Du, Jiaxi Xu, Zhanhui Yang
Summary: A practical and efficient synthesis of 11-aryldibenzooxa/thiazepines is achieved by a late-stage and two-step C11-H arylation of simple dibenzooxa/thiazepines. The use of Grignard addition and DDQ dehydrogenation enables operationally simple and reliable transformations with high yields. The two-step and one-pot procedures demonstrate excellent yields and the gram-scale experiment highlights its potential for large-scale applications and efficient synthesis of H460TaxR inhibitors.
SYNTHESIS-STUTTGART
(2023)
Article
Microbiology
Hui Zhao, Yue Su, Qihui Gu, Jumei Zhang, Xinqiang Xie, Qingping Wu
Summary: A Gram-positive, facultatively anaerobic, agar-hydrolytic and rod-shaped bacterium named strain SCIV0701T was isolated from soya bean rhizosphere soil in Sichuan Province, China. It belongs to the genus Paenibacillus and is genetically distinct from its closely related species. Therefore, a new species named Paenibacillus soyae sp. nov. is proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Mouna Dammak, Hajer Ben Hlima, Fatma Elleuch, Chantal Pichon, Philippe Michaud, Imen Fendri, Slim Abdelkafi
Summary: Tetraselmis sp. strain MD-01 has been studied for autotrophic lipids production with a two-stage process, showing potential for biodiesel production by effectively increasing lipid content in cells through the two-stage strategy.
Article
Chemistry, Physical
Ya-Ning Yang, Chao-Wei Huang, Van-Huy Nguyen, Jeffrey C. -S. Wu
Summary: Methanol can be produced from CO2 hydrogenation through a two-stage reaction process, leading to significantly higher yield compared to a single-stage reaction. In the first stage at high temperatures, CO2 is hydrogenated to CO and H2O, followed by further hydrogenation of CO to CH3OH in the second stage at low temperatures. This concept enables the transformation of CO2 hydrogenation towards increased CH3OH production.
CATALYSIS COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Mallique Qader, K. H. Ahammad Uz Zaman, Zhenquan Hu, Cong Wang, Xiaohua Wu, Shugeng Cao
Summary: A new compound, aspochalasin H1, with unique structural features, was isolated from Aspergillus sp. FT1307. The isolated compounds showed diverse biological activities against pathogenic bacteria and cancer cell lines.
Article
Cell Biology
Ming-Hua Liang, Shan-Rong Xie, Hao-Hong Chen, Jian-Guo Jiang
Summary: In this study, a nucleus-localized MADS transcription factor (DbMADS) was found to negatively regulate carotenoid biosynthesis in algae by repressing the transcriptional activity of phytoene synthase (DbPSY) and lycopene beta-cyclase (DbLcyB). This study provides new insights into the regulatory mechanisms of carotenoid metabolism in algae.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Antonio Garcia-Moyano, Yuleima Diaz, Jose Navarro, David Almendral, Pal Puntervoll, Manuel Ferrer, Gro Elin Kjaereng Bjerga
Summary: To support the development of environment-friendly processes and products in the bio-based industry, an optimal toolbox of biocatalysts is crucial. Functional screen of (meta)genomic libraries can potentially identify new enzymes, but discovering enzymes that meet industry compliance demands remains challenging, especially in the case of proteases. This study demonstrated the use of six industry-relevant animal and plant by-products as complementary substrates in functional screens, suggesting temperature as a screening parameter to potentially discover new broad-substrate range and robust proteases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)