Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 150, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2020.104955
Keywords
Jiaozhou bay; Denitrification activity; Community composition; Surface sediment
Funding
- National Natural Science Foundation of China [91851111, 31870100]
- Natural Science Foundation of Guangdong Province [2019B1515120066]
- Research Foundation for Talented Scholars of Guangzhou University [GU2017001]
- Graduate Innovative Research Grant Program of Guangzhou University [019GDJC-M09]
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Denitrification plays a dominant role in the removal of reactive inorganic nitrogen species in different ecosystems. In this study, we investigated the activity, abundance, and community composition of nirS-encoding denitrifying bacteria from sediments of Jiaozhou Bay (JZB). The rates of denitrification, measured by N-15 amendment and incubation experiments, varied from 0.18 to 9.97 nmol N g(-1) h(-1) and contributed to 81.45-99.93% of the total N-2 production, while the estimated flux of nitrogen removal was about 5.66 x 10(4) t N y(-1) in surface sediments. Additionally, the abundance of nirS gene ranged from 5.20 x 10(5) to 3.01 x 10(8) copies per gram. High-throughput sequencing technique was used to target the nirS gene, which revealed 19 dominant OTU5 at 93% similarity that were assigned to 7 clusters. Cluster 2 was mainly affiliated to Thiothrix, while Cluster 4 was mainly affiliated to Woeseia and the other clusters were similar to uncultured denitrifiers obtained from sediments of several coastal estuaries and bays. NH4+ and NO3- contents were identified as the two main factors affecting community composition and distribution of denitrifiers among the surface sediments of JZB. Overall, our results showed that the community of nirS-type denitrifying bacteria had a diverse composition and could highly contribute to nitrogen loss in eutrophic systems.
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