Journal
BIOTECHNOLOGY LETTERS
Volume 38, Issue 1, Pages 81-88Publisher
SPRINGER
DOI: 10.1007/s10529-015-1945-7
Keywords
Biosynthesis; Caffeoyl-phenyllactate; 4-Coumarate CoA ligase; Escherichia coli; Rosmarinic acid; Rosmarinic acid synthase
Categories
Funding
- 973 Program of China [2012CB721100]
- 863 Program of China [2012AA02A704]
- 100 Talents Program of the Chinese Academy of Sciences
- National Natural Science Foundation of China [31300040, 21302214]
Ask authors/readers for more resources
To achieve high production of rosmarinic acid and derivatives in Escherichia coli which are important phenolic acids found in plants, and display diverse biological activities. The synthesis of rosmarinic acid was achieved by feeding caffeic acid and constructing an artificial pathway for 3,4-dihydroxyphenyllactic acid. Genes encoding the following enzymes: rosmarinic acid synthase from Coleus blumei, 4-coumarate: CoA ligase from Arabidopsis thaliana, 4-hydroxyphenyllactate 3-hydroxylase from E. coli and d-lactate dehydrogenase from Lactobacillus pentosus, were overexpressed in an l-tyrosine over-producing E. coli strain. The yield of rosmarinic acid reached similar to 130 mg l(-1) in the recombinant strain. In addition, a new intermediate, caffeoyl-phenyllactate (similar to 55 mg l(-1)), was also produced by the engineered E. coli strain. This work not only leads to high yield production of rosmarinic acid and analogues, but also sheds new light on the construction of the pathway of rosmarinic acid in E. coli.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available