Article
Chemistry, Applied
Miriam Hidalgo-Serrano, Francesc Borrull, Rosa Maria Marce, Eva Pocurull
Summary: This article presents a new analytical method for simultaneously extracting multiple phthalate diesters and monoesters from highly consumed seafood, which has been validated for seafood with different lipid contents. The method showed high apparent recoveries and low matrix effect values for all compounds, with detection limits ranging from 1-25 ng g(-1) dry weight. Five seafood species were successfully analyzed using this method, revealing concentrations of phthalate diesters up to 982 ng g(-1) (d.w.) and phthalate monoesters up to 178 ng g(-1) (d.w.).
Article
Biochemistry & Molecular Biology
Xiaoyang Yan, Qiaoling Zhao, Zhongyong Yan, Xuechang Chen, Pengfei He, Shiyan Li, Yi Fang
Summary: This study developed and validated a sensitive UPLC-MS/MS method for the determination of PCP in seafood samples. PCP was detected in 5.8% of 760 seafood samples from Zhejiang Province, with the largest portion of detections found in shellfish, accounting for approximately 60% of the total. The non-carcinogenic risk indices for adults and children who consume PCP ranged from 10(-4) to 10(-3), indicating minimal health risk from PCP in marine organisms to humans.
Article
Chemistry, Analytical
Luxiu Li, Ya Cui, Lingling Ren, Chunyang Li, Yehao Liu, Zhongxiu Jin
Summary: A simple and efficient dispersive solid-phase microextraction method based on magnetic dummy molecularly imprinted polymer nanoparticles was developed for the extraction of five phthalate monoesters from human urine samples. The prepared nanoparticles showed high selectivity, good adsorption capacity, excellent reusability, and fast adsorption rates. Furthermore, the method demonstrated good linearity and recovery rates for all five phthalate monoesters in human urine samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Miriam Hidalgo-Serrano, Francesc Borrull, Eva Pocurull, Rosa Maria Marce
Summary: Organophosphate triesters are widely used in industries and can be transformed into organophosphate diesters in the environment. Some organophosphate diesters can be toxic and mutagenic. Therefore, it is important to study their presence in widely consumed seafood. In this study, an analytical method for determining organophosphate diesters in seafood was developed and validated.
Article
Chemistry, Analytical
Youwen You, Rachel M. Proctor, Kevin Guo, Xiaoqing Li, Evan Xue, Fuyu Guan, Mary A. Robinson
Summary: HRMS is a powerful technology for equine doping control analysis, with the newly developed Orbitrap-based HRMS instrument allowing for targeted and non-targeted screening analyses. A validated LC-HRMS/MS method was developed to detect prohibited substances in equine sports, utilizing a screening strategy and specific criteria to increase method specificity. This novel methodology provides a simple, rapid, and reproducible strategy for identifying doping substances in horse racing.
ANALYTICAL METHODS
(2021)
Article
Biochemistry & Molecular Biology
Zuzana Jurcackova, Denisa Ciglanova, Dagmar Mudronova, Lenka Tumova, Daniela Barcenas-Perez, Jiri Kopecky, Jana Koscova, Jose Cheel, Gabriela Hrckova
Summary: This study investigates the bio-functional effects of an astaxanthin-rich extract (EXT) and its fractions, astaxanthin monoesters-rich fraction (ME) and astaxanthin diesters-rich fraction (DE), on cellular functions and signaling pathways. The results show that co-cultivation with EXT, ME, and DE can restore or stimulate cell functions under standard culture conditions. Furthermore, the study highlights the importance of selecting physioxia-like conditions for pharmacological research.
Article
Medical Laboratory Technology
Sophie C. Anker, Jakob Morgenstern, Jakob Adler, Maik Brune, Sebastian Brings, Thomas Fleming, Elisabeth Kliemank, Markus Zorn, Andreas Fischer, Julia Szendroedi, Lars Kihm, Johanna Zemva
Summary: This feasibility study investigates whether indirect methods based on routine laboratory results can be used to verify reference intervals for steroid hormones. The study shows that indirect methods are a valuable tool to verify existing reference intervals. The results indicate a good agreement between indirectly and directly determined reference intervals.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Engineering, Chemical
Celia Sanchez-Vallejo, Ana Ballesteros-Gomez, Soledad Rubio
Summary: Polyphenols are highly appreciated antioxidants. The extraction of polyphenols from biomass is currently inefficient and not eco-friendly. Supramolecular solvents (SUPRAS) have shown great potential in efficient and environmentally friendly extraction of polyphenols.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Viviane Silva Siqueira Sandrin, Gabriela Moraes Oliveira, Giovana Maria Weckwerth, Nelson Leonel Del Hierro Polanco, Flivio Augusto Cardoso Faria, Carlos Ferreira Santos, Adriana Maria Calvo
Summary: This study aimed to systematically investigate and analyze different drug extraction methods for Liquid Chromatography in Mass Spectrometry assays (LC-MS/MS), focusing on non-steroidal anti-inflammatory drugs in biological fluid samples. Through a comprehensive search and evaluation of literature, it was found that liquid-liquid extraction (LLE) and solid-phase extraction (SPE) were the main methods used. Additionally, modifications of these traditional techniques were also validated for use in LC-MS/MS. The study suggests that future research should explore more ecological, economic, and sustainable extraction approaches.
Article
Biochemical Research Methods
Kalpana Talari, Sai Krishna Ganji, Mani Kommu, Raja Rajeswari Tiruveedula, Vijayasarathi Upadhyayula
Summary: A simple, selective, and sensitive method based on LC-APCI-HCD-HRMS/MS was developed for quantitative determination of 19 targeted organophosphorus and carbamate pesticides as well as 10 untargeted pesticides in coconut milk samples. The method showed good linearity, sensitivity, and precision, with successful quantification of pesticide residues in the samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Engineering, Chemical
Ana Ballesteros-Gomez, Antonio Serrano-Crespin, Soledad Rubio
Summary: In this study, a simple liquid-liquid extraction method using green supramolecular solvents was developed for the extraction of hydroxytyrosol from brines of table olive processing. The optimized extraction conditions were found to be 4.8% v/v 1,2-hexanediol and 1 M Na2SO4. The final extracts were further analyzed to identify other compounds of interest.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Lixy Leon-Moran, Marta Pastor-Belda, Natalia Campillo, Natalia Arroyo-Manzanares, Carmen Torres, Pilar Vinas
Summary: This study aimed to develop a novel analytical method for the determination of metabolites of persistent organic pollutants in human tissues. The method achieved good precision and demonstrated no detection of the target compounds in the studied samples.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Sylwia Studzinska, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska
Summary: The present study compared three different liquid chromatography modes for the qualitative analysis of nusinersen metabolites and found that hydrophilic interaction liquid chromatography detected the smallest number of metabolites. The response of mass spectrometry was higher for ion pair chromatography compared to reversed-phase chromatography. Various extraction methods were used, and it was found that hybridization allowed selective extraction of nusinersen analogs while weak ion exchange-based extraction was highly efficient.
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Yuya Cheng, Peize Wu, Ying Kan, Ming Li, Hongmei Li
Summary: In this study, liquid chromatography-high-resolution mass spectrometry methods were used to comprehensively characterize and quantify thymalfasin-related impurities. The results showed that there are various impurities in thymalfasin standard and commercial materials, with over half of them related to the C-terminal Asn residue. Therefore, it is proposed to modify or replace the C-terminal Asn residue of thymalfasin for new drug research and development.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Nuria Fontanals, Francesc Borrull, Rosa Maria Marce
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
Josep Borrull, Agusti Colom, Josepa Fabregas, Eva Pocurull, Francesc Borrull
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Environmental Sciences
Laura Vallecillos, Anna Borrull, Rosa Maria Marce, Francesc Borrull
Summary: Passive sampling combined with thermal desorption gas chromatography-mass spectrometry (TD-GC-MS) was used to monitor emerging organic contaminants (EOCs) and solvents in indoor environments of both conventional and vocational training schools. Experimental diffusive uptake rates of target compounds were determined successfully, and concentrations of EOCs and solvents were found to be higher in the vocational training school compared to the conventional school. Cleaning products (conventional school) and classroom activities (vocational training school) were identified as the main sources of EOCs and solvents.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Applied
Miriam Hidalgo-Serrano, Francesc Borrull, Rosa Maria Marce, Eva Pocurull
Summary: This article presents a new analytical method for simultaneously extracting multiple phthalate diesters and monoesters from highly consumed seafood, which has been validated for seafood with different lipid contents. The method showed high apparent recoveries and low matrix effect values for all compounds, with detection limits ranging from 1-25 ng g(-1) dry weight. Five seafood species were successfully analyzed using this method, revealing concentrations of phthalate diesters up to 982 ng g(-1) (d.w.) and phthalate monoesters up to 178 ng g(-1) (d.w.).
Article
Engineering, Environmental
Iria Gonzalez-Marino, Leticia Ares, Rosa Montes, Rosario Rodil, Rafael Cela, Ester Lopez-Garcia, Cristina Postigo, Miren Lopez de Alda, Eva Pocurull, Rosa Maria Marce, Lubertus Bijlsma, Felix Hernandez, Yolanda Pico, Vicente Andreu, Andreu Rico, Yolanda Valcarcel, Manuel Miro, Nestor Etxebarria, Jose Benito Quintana
Summary: This study estimated human exposure to phthalates in the Spanish population using a wastewater-based epidemiology approach, revealing that some metabolite exposure levels exceeded the daily exposure thresholds set by the European Food Safety Authority.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Lubertus Bijlsma, Yolanda Pico, Vicente Andreu, Alberto Celma, Andrea Estevez-Danta, Iria Gonzalez-Marino, Felix Hernandez, Miren Lopez de Alda, Ester Lopez-Garcia, Rosa Maria Marce, Manuel Miro, Rosa Montes, Unai Perez de San Roman-Landa, Elena Pitarch, Eva Pocurull, Cristina Postigo, Ailette Prieto, Andreu Rico, Rosario Rodil, Yolanda Valcarcel, Mireia Ventura, Jose Benito Quintana
Summary: The data obtained from wastewater analysis can provide rapid insights into illicit drug consumption at the community level. A national wastewater monitoring program was implemented in Spain to understand spatial differences and trends in drug use, with cannabis and cocaine being the most commonly consumed drugs in the country. Regional variations were observed, such as higher levels of methamphetamine in Barcelona and amphetamine in Bilbao, indicating different primary drugs in these metropolitan areas.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Nuria Fontanals, Eva Pocurull, Francesc Borrull, Rosa Maria Marce
Summary: Pressurized liquid extraction (PLE) is a widely used extraction technique for determining contaminants in environmental solid samples. Clean-up strategies are essential to remove interferences and avoid matrix effects. Different clean-up strategies have been used in PLE over the past 10 years, and this review critically discusses their strengths and weaknesses to help readers select the most suitable technique for a specific environmental analytical problem.
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yandi Fu, Francesc Borrull, Rosa Maria Marce, Naria Fontanals
Summary: This review discusses the determination and analysis of enantiomeric fractions of chiral drugs in environmental matrices using chiral liquid chromatography coupled with mass spectrometry. It provides a comprehensive overview and comparison of various techniques, addresses advancements and limitations, and explores the impact of the mobile phase composition on enantioseparation and MS detection. Various applications of chiral LC and MS for enantiomeric fraction determination of chiral drugs in environmental samples are also discussed in depth.
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
N. Fontanals, J. Zohar, F. Borrull, S. Ronka, R. M. Marce
Summary: This study introduces a novel mixed-mode weak cation-exchange (WCX) material prepared by functionalizing a mesoporous divinylbenzene (DVB) resin with maleic acid. The material showed good recovery rates and low matrix effects in the solid-phase extraction (SPE) of basic drugs from environmental water samples. The optimized protocol coupled with high resolution mass spectrometry (HRMS) proved effective in quantifying basic drugs in different environmental water samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Environmental Sciences
Mireia Nunez, Nuria Fontanals, Francesc Borrull, Rosa Maria Marcie
Summary: A multiresidue analytical method was developed to determine 22 compounds in indoor dust. Organophosphate esters and phthalate esters were the most abundant compounds, with DEHP being found at the highest concentrations. Risk assessments showed no risks in most scenarios, except for possible carcinogenic risk from ingestion of DEHP in the worst-case scenario.
Article
Biochemical Research Methods
Alberto Moral, Francesc Borrull, Kenneth G. Furton, Abuzar Kabir, Nuria Fontanals, Rosa Maria Marce
Summary: In this study, four novel mixed-mode zwitterionic silica-based sorbents were synthesized and evaluated for the determination of acidic and basic drugs in environmental water samples. The sorbents showed effective retention of both acidic and basic compounds, with selective retention of basic compounds achieved through ionic exchange interactions. The optimized solid-phase extraction (SPE) method using the best-performing sorbents was validated for the analysis of river and effluent wastewater samples, demonstrating satisfactory recoveries, low detection limits, and good repeatability and reproducibility.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Environmental Sciences
Alba Maceira, Francesc Borrull, Rosa Maria Marce
Summary: This paper examines the occurrence of organic contaminants bonded to particulate matter in ambient air, focusing on their sources, fates, and distributions. It emphasizes the importance of being aware of the presence of these contaminants in outdoor air for establishing new guidelines and reassessing existing limitations.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Laura Vallecillos, Rosa Maria Marce, Francesc Borrull
Summary: This study focuses on the implementation of a gas chromatograph-photoionization detection (GC-PID) analyzer for the continuous monitoring of 1,3-butadiene (1,3-BD) levels in urban and industrial atmospheres. The study found that the concentrations of 1,3-BD recorded by the GC-PID analyzer were comparable to those obtained by active sampling, and the concentration peaks showed consistency in values and time slots. In the test of urban atmospheres, the results showed that the concentrations of 1,3-BD were related to prevailing wind direction and activities in the petrochemical zone, while other factors had minor effects on the distribution of this pollutant.
ATMOSPHERIC POLLUTION RESEARCH
(2024)
