Article
Medicine, Research & Experimental
Ying Li, Suyu Wang, Yihan Xiao, Bing Liu, Jianliang Pang
Summary: Nerve growth factor (NGF) shows potential application in diabetic periodontitis by inhibiting MSC apoptosis induced by high glucose, reducing the proportion of MSCs transformed into proinflammatory type, and enhancing the therapeutic effect of MSCs on diabetic periodontitis.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Cell & Tissue Engineering
Kangkang Zha, Yu Yang, Guangzhao Tian, Zhiqiang Sun, Zhen Yang, Xu Li, Xiang Sui, Shuyun Liu, Jinmin Zhao, Quanyi Guo
Summary: The nerve growth factor (NGF) and its receptors TrkA and p75NTR play important roles in various aspects of mesenchymal stem/stromal cell (MSC) function, including survival, growth, differentiation, and angiogenesis. The regulatory effect of NGF on MSCs is mainly achieved through binding to TrkA, while p75NTR is considered a novel surface marker of MSCs. This understanding provides new insights for optimizing MSC-based therapy.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Neurosciences
Ziying Zhu, Xiaona Zhang, Haojie Hao, Heran Xu, Jun Shu, Qian Hou, Min Wang
Summary: Exosomes derived from umbilical cord mesenchymal stem cells (UC-MSCs) play a key role in promoting wound repair and skin regeneration by recruiting and stimulating fibroblasts to secrete nerve growth factors (NGFs) for skin nerve repair.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Cell & Tissue Engineering
Ping Wang, Yan Cui, Jing Wang, Donghua Liu, Yue Tian, Kai Liu, Xue Wang, Lin Liu, Yu He, Yufeng Pei, Li Li, Liying Sun, Zhijun Zhu, Dehua Chang, Jidong Jia, Hong You
Summary: This study compared the therapeutic effects of mesenchymal stem cells (MSCs) and N-acetylcysteine (NAC) on acetaminophen (APAP)-induced liver failure and explored the underlying mechanism. The results showed that MSCs had comparable therapeutic effects to NAC, but had slightly different effects on hepatocyte necrosis and cell infiltration, and attenuated hepatocyte necrosis by secreting hepatocyte growth factor (HGF). These findings provide a theoretical basis for the clinical application of MSCs in treating APAP liver failure.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Ebtehal Ahmed, Tarek Saleh, Meifeng Xu
Summary: Researchers are exploring the use of mesenchymal stem cells (MSCs) as alternative cells for repopulating decellularized scaffolds. MSCs, derived from various sources, have therapeutic effects on a wide range of inflammatory/degenerative diseases. Overcoming the drawbacks of MSC recellularization is essential for generating bioengineered transplantable organs.
Article
Biochemistry & Molecular Biology
Woo-Sup Sim, Bong-Woo Park, Kiwon Ban, Hun-Jun Park
Summary: The study explored a novel therapeutic platform called in situ preconditioning to enhance the therapeutic potential of BM-MSCs in a rat myocardial infarction model. Results showed that this approach significantly improved the survival and retention of BM-MSCs in the heart, leading to enhanced cardiac function and repair in myocardial infarction hearts. This suggests that in situ preconditioning could be an effective strategy for cardiac regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Fang Chen, Yingxin Gong, Ninghong Jiang, Jingjing Xiao, Yaping Wang, Limei Chen, Long Sui
Summary: This study found that collagen scaffolds loaded with bFGF-BMSCs significantly increased the endometrial thickness, gland number, and microvascular density, promoting the regeneration of injured endometrium. The expression levels of bFGF, VEGF, vimentin, and Ki67 were higher in the bFGF-BMSC+scaffold group compared to the BMSC+scaffold group. These findings contribute to a better understanding of stem cell treatment for intrauterine adhesion.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2022)
Review
Materials Science, Multidisciplinary
Seunghun S. Lee, Xiaoyu Du, Inseon Kim, Stephen J. Ferguson
Summary: Due to increased life expectancy, there is a growing demand for efficient orthopedic treatment to address the rising number of orthopedic-related fractures and pathologies. Bone-tissue engineering (BTE) has emerged as an innovative solution using scaffolds to regenerate bone tissue. This article provides an overview of the characteristics of ideal scaffolds and categorizes biomaterials for BTE scaffolds. Recent advances in scaffolds developed over the past 10 years are also reviewed.
Article
Cardiac & Cardiovascular Systems
Wei Luo, Yanshan Gong, Fan Qiu, Yi Yuan, Wenwen Jia, Zhongmin Liu, Ling Gao
Summary: NGF PLGA nanoparticles can protect hUCMSCs and promote their paracrine effects through the TrkA-PI3K/Akt signaling pathway, similar to free NGF. The application of NGF PLGA nanoparticles in hUCMSC fibrin cardiac patches significantly improves the retention of transplanted hUCMSCs and enhances their ability to reduce myocardial apoptosis and promote angiogenesis in the mouse heart after MI.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Polymer Science
Qiuxia Li, Litao Sun, Xiaobei Huang, Shuai Liu, Haiyang Yong, Chenfei Wang, Jianzhong Li, Dezhong Zhou
Summary: Biodegradable and lipid-like highly branched poly(beta-amino ester)s (HPAESA) were developed to enhance the biological functions of adipose-derived stem cells by gene transfection. HPAESA showed significantly higher efficiency and cell viability, as well as the ability to promote neurite outgrowth.
Review
Cell & Tissue Engineering
Xiangling Li, Yanjun Guan, Chaochao Li, Tieyuan Zhang, Fanqi Meng, Jian Zhang, Junyang Li, Shengfeng Chen, Qi Wang, Yi Wang, Jiang Peng, Jinshu Tang
Summary: The article summarizes the interactions between MSCs, immune cells, and inflammatory cytokines following PNI, with a focus on the immunosuppressive role of MSCs. Additionally, the immunomodulatory potential of MSC-derived extracellular vesicles as a cell-free treatment for PNI is discussed.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Dongyuan Luo, Boying Chen, Yu Chen
Summary: The currently available biomaterials for alveolar cleft repair have poor mechanical properties and biocompatibility. In this study, a 3D printed biomimetic scaffold was developed using a mixture of beta-tricalcium phosphate (beta-TCP) and bone marrow mesenchymal stem cells (BMSCs). The hybrid scaffold showed good biocompatibility and mechanical properties, and supported the attachment, proliferation, survival, differentiation, and osteogenic induction of BMSCs for alveolar cleft reconstruction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Dominik Steiner, Lea Reinhardt, Laura Fischer, Vanessa Popp, Carolin Koerner, Carol Geppert, Tobias Baeuerle, Raymund E. Horch, Andreas Arkudas
Summary: The microvascular endothelial network is crucial for osteogenesis and bone tissue engineering. Endothelial progenitor cells (EPCs) have a strong angiogenic potential and can enhance the vascularization and tissue formation of scaffolds. This study investigated the effects of EPCs on the vascularization and bone formation of a hydroxyapatite and beta-tricalcium phosphate-fibrin scaffold. The results showed successful scaffold vascularization in all groups, and the EPC/MSC coimplantation group demonstrated bone formation. The arteriovenous loop was found to be an effective method for vascularization independent of scaffold cellularization with EPCs.
Article
Materials Science, Multidisciplinary
Yanhong Pei, Lifei Huang, Tong Wang, Qinhan Yao, Yanrong Sun, Yan Zhang, Xiaomei Yang, Jiliang Zhai, Lihua Qin, Jiajia Xue, Xing Wang, Hongquan Zhang, Junhao Yan
Summary: Researchers have discovered that loading bone marrow mesenchymal stem cells (BMSCs) in rigid-flexible composite scaffolds can significantly reduce brain edema and infarct volume while improving neurological deficits after stroke. This study presents a promising approach for treating central nervous system diseases through cell transplantation.
MATERIALS TODAY ADVANCES
(2023)
Article
Biochemistry & Molecular Biology
Rimma Sergeevna Kamentseva, Marianna Viktorovna Kharchenko, Gulnara Vladikovna Gabdrahmanova, Michael Alexandrovich Kotov, Vera Vladislavovna Kosheverova, Elena Sergeevna Kornilova
Summary: This study compared the effects of EGF, TGF-α, and AREG on physiological responses in endometrial MSCs and HeLa cells, and investigated the internalization and degradation of EGFR stimulated by these ligands. The study found that while EGF and TGF-α stimulated cell proliferation and prevented differentiation in enMSCs in an EGFR-dependent manner, AREG had no effect. Additionally, differences were observed in the degradation and recovery of EGFR in different cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Medicinal
Debora Renz Barreto Vianna, Jessica Gotardi, Simone Cristina Baggio Gnoatto, Diogo Andre Pilger
Summary: Chronic myeloid leukemia (CML) is characterized by the oncoprotein BCR-ABL1 and is mainly treated with tyrosine kinase inhibitors. Pentacyclic triterpenes, with antitumoral properties, have shown promising pro-apoptotic and antiproliferative activities in CML cells, making them a rich source for drug development in CML treatment.
Article
Biochemistry & Molecular Biology
Isabele Cristiana Iser, Paola de Andrade Mello, Samuel Davies, Jacqueline Fraga de Souza Santos, Diogo Andre Pilger, Andreia Buffon, Ana Paula Santin Bertoni, Marcia Rosangela Wink
Summary: The study investigated the expression and activity of CD73 in cervical cancer stem-like cells, showing a decreased level of CD73 in these cells compared to monolayer cells, which was restored when these cells were re-plated as monolayers. In addition, in silico analysis revealed a similar pattern in CSC-like populations derived from cervical, thyroid, and breast cancer, indicating the potential importance of CD73 as a therapeutic target in cervical cancer.
CELL BIOCHEMISTRY AND FUNCTION
(2021)
Article
Biochemistry & Molecular Biology
Elenilson F. da Silva, Artur S. de Vargas, Julia B. Willig, Cristiane B. de Oliveira, Aline R. Zimmer, Diogo A. Pilger, Andreia Buffon, Simone C. B. Gnoatto
Summary: This study evaluated the effects of pharmacomodulations on the activity against K562 leukemia cells of triterpene derivatives of betulinic and ursolic acids, resulting in the identification of 2c derivative as the most active with a good selectivity index for cancer over normal cells. The 2c derivative induced apoptosis and cell cycle arrest, showing synergy with imatinib, providing a strong rationale for further investigation of combination therapy in pre-clinical studies.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Editorial Material
Otorhinolaryngology
Adilson Marcos Montefusco, Geraldo Pereira Jotz
INTERNATIONAL ARCHIVES OF OTORHINOLARYNGOLOGY
(2021)
Editorial Material
Otorhinolaryngology
Geraldo Pereira Jotz, Aline Gomes Bittencourt, Adilson Marcos Montefusco
INTERNATIONAL ARCHIVES OF OTORHINOLARYNGOLOGY
(2022)
Editorial Material
Otorhinolaryngology
Fatima Cristina Mendes de Matos, Geraldo Pereira Jotz
INTERNATIONAL ARCHIVES OF OTORHINOLARYNGOLOGY
(2021)
Article
Medical Laboratory Technology
Pamela Rossi Menegotto, Mariela Granero Farias, Fabiane Spagnol, Marina Siebert, Eduardo Cremonese Filippi-Chiela, Ana Paula Alegretti, Diogo Andre Pilger
Summary: This study compared three non-sequencing protocols for detecting major NPM1 mutations and evaluated nuclear morphometric analysis as an alternative method for cuplike blast detection. The results showed that quantitative PCR (qPCR) is the best option to identify NPM1 mutations, with ARMS-PCR being a cheaper alternative. Multiparameter flow cytometry (MFC) can be used as a screening method.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2023)
Editorial Material
Otorhinolaryngology
Geraldo Pereira Jotz, Aline Gomes Bittencourt, Adilson Marcos Montefusco
INTERNATIONAL ARCHIVES OF OTORHINOLARYNGOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Carolina Bissani Gasparin, Diogo Andre Pilger
Summary: 8-Hydroxyquinoline (8HQ) and its derivatives are metal-binding ligands capable of forming complexes with various biological activities. Our study summarizes the antileukemia activities of 8HQ compounds by discussing their mechanisms of action and compiling cytotoxicity data against different leukemia cell lines. The pharmacological activities of these compounds are mainly attributed to their metal coordination ability and interaction with multiple biological targets. We found consistent evidence that 8HQ exhibits promising proapoptotic features by directly interacting with DNA, inhibiting several targets, and inducing autophagy-associated cell death. These findings support the potential repositioning of 8HQ compounds for leukemia treatment, addressing the need for advances in therapeutic approaches.
Article
Chemistry, Medicinal
Julia Biz Willig, Nadia Mileo Garces de Couto, Debora Renz Barreto Vianna, Camila da Silveira Mariot, Simone Cristina Baggio Gnoatto, Andreia Buffon, Diogo Andre Pilger
Summary: This study investigated the cytotoxic activity and mechanism of action of a hybrid compound in chronic myeloid leukemia cell lines that are resistant to imatinib. The compound exhibited cytotoxicity and showed a synergistic effect when combined with imatinib. The compound induced cell death through apoptosis, cell cycle arrest, autophagy, and increased oxidative stress.
Article
Pharmacology & Pharmacy
Jessica Nascimento, Isadora do Canto Olegario, Camila Mariot, Thayse Viana de Oliveira, Paula dos Santos Chaves, Rafaela Oliveira, Edilene Gadelha de Oliveira, Silvia Staniscuaski Guterres, Andreia Buffon, Diogo Andre Pilger, Ruy Carlos Ruver Beck
Summary: In this study, the encapsulation of orlistat (ORL) in polymeric aqueous nanocapsules (NC) was evaluated for its in vitro cytotoxic activity against cervical cancer cells. The nanoencapsulation improved the cytotoxic effect of ORL, suggesting its potential as a treatment for cervical cancer.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jessica Nascimento, Camila Mariot, Debora R. B. Vianna, Lucia M. Kliemann, Paula S. Chaves, Massimo Loda, Andreia Buffon, Ruy C. R. Beck, Diogo A. Pilger
Summary: This study investigated the expression of Fatty acid synthase (FASN) in premalignant lesions and cervical cancer samples, as well as the effects of a FASN inhibitor on cervical cancer cells. The results showed that FASN was expressed in all cervical cancer samples and its expression increased with advanced stages of cervical cancer. The FASN inhibitor orlistat reduced the viability of cervical cancer cells in a time-dependent manner and induced apoptosis, cell cycle arrest, and autophagy.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Article
Audiology & Speech-Language Pathology
Annelise Ayres, Pablo Brea Winckler, Lais Alves Jacinto-Scudeiro, Rafaela Soares Rech, Marina Martins Pereira Padovani, Geraldo Pereira Jotz, Maira Rozenfeld Olchik
Summary: The study aims to describe the speech pattern of patients with Myasthenia Gravis (MG) and compare it with healthy controls. The results show that MG patients have mild dysarthria, with significant changes in breathing and phonation. The study also finds significant differences in certain speech sounds between the MG group and the healthy control group.
LOGOPEDICS PHONIATRICS VOCOLOGY
(2022)
Article
Otorhinolaryngology
Geraldo Pereira Jotz, Fatima Cristina Mendes de Matos
INTERNATIONAL ARCHIVES OF OTORHINOLARYNGOLOGY
(2021)
Article
Infectious Diseases
Aline Daniele Schuster, Debora Renz Barreto Vianna, Lucia Maria Kliemann, Marcia Luiza Montalvao Appel Binda, Luciane Noal Calil, Diogo Andre Pilger, Andreia Buffon
REVISTA DE EPIDEMIOLOGIA E CONTROLE DE INFECCAO
(2020)
