Review
Chemistry, Multidisciplinary
Jingwen Zhuang, Ruiyue Hang, Ruoyue Sun, Yanshu Ding, Xiaohong Yao, Ruiqiang Hang, Hui Sun, Long Bai
Summary: Exosomes secreted by bone marrow mesenchymal stem cells have multifaceted effects on osseointegration and hold great promise as a safe, efficient, and ethically unrestricted therapy.
FRONTIERS IN CHEMISTRY
(2022)
Review
Cell Biology
Xinyun Xu, Ling Zhao, Paul D. Terry, Jiangang Chen
Summary: Mesenchymal stem cells from bone marrow can differentiate into either adipocytes or osteoblasts, and external stimuli such as environmental contaminants, heavy metals, dietary, and physical factors can influence this fate decision. Maintaining a balance between osteogenesis and adipogenesis is crucial for bone homeostasis, and disruptions in lineage commitment of these stem cells are associated with various bone disorders. This review focuses on understanding how external stimuli affect the fate of mesenchymal stem cells and calls for future studies to investigate the impact on bone health and underlying mechanisms of differentiation.
Article
Cell Biology
Xiaoyu Li, Xue Wang, Chunmei Zhang, Jinsong Wang, Songlin Wang, Lei Hu
Summary: This study investigated the metabolic activity of MSCs and its possible mechanism during aging. The findings suggest that the metabolic function of BMSCs is impaired in aged mice, characterized by decreased oxidative phosphorylation and glycolytic activity, as well as lower mitochondrial membrane potential, NAD+/NADH ratio, and ATP production. FABP4 may play a key role in BMSC senescence caused by fatty acid metabolism disorders. Regulation of metabolic activity could be a potential therapeutic target for enhancing the regenerative functions of BMSCs.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Haitao Wang, Peng Wei, Yi Zhang, Yuebai Li, Li Yin
Summary: lncRNA plays a significant role in osteoporosis, regulating osteogenic differentiation, adipogenic differentiation, and osteogenic-angiogenic coupling of bone marrow mesenchymal stem cells through the TCONS_00023297/miR-608/RUNX2/SHH signaling axis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Meng Li, Jing Wu, Wenbo Geng, Yulu Yang, Xuan Li, Kun Xu, Ke Li, Yan Li, Qiaojian Duan, Pengfei Gao, Kaiyong Cai
Summary: Localized corrosion of 316L stainless steel reduces the viability, adhesion, proliferation, and osteogenic differentiation abilities of BMSCs. It causes cytotoxicity through the release of metallic ions and generates reactive oxygen species (ROS) around the corrosion pits, leading to oxidative stress and cell membrane depolarization. This study provides insights into corrosion-mediated degradation of cell function and material-cell interactions.
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Review
Biochemistry & Molecular Biology
Laidi Wu, Zhixin Liu, Li Xiao, Mi Ai, Yingguang Cao, Jing Mao, Ke Song
Summary: This review comprehensively elucidates the specific roles of Gli1+ MSCs in craniofacial bone osteogenesis and tissue repair, offering new insights into bone regeneration therapy.
Article
Endocrinology & Metabolism
Ziyi Li, Yafei Wang, Shilun Li, Yukun Li
Summary: Bone regeneration is a complex process involving the coordination of osteogenesis and osteoclastogenesis, with the balance between osteogenesis and adipogenesis of BMSCs playing a critical role. Recent studies have shown that exosomes from M2 macrophages facilitate osteogenesis and reduce adipogenesis through the miR-690/IRS-1/TAZ axis, potentially serving as a therapeutic tool for bone loss diseases.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tongtong Chen, Hanqi Wang, Chaoyin Jiang, Yong Lu
Summary: This study reveals that under oxidative stress conditions, PKD1 may alleviate the inhibitory effect of oxidative stress on osteogenesis of rat BM-MSCs through TAZ activation.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Engineering, Biomedical
Qij Lu, Jingjing Diao, Yingqu Wang, Jianlang Feng, Fansen Zeng, Yan Yang, Yudi Kuang, Naru Zhao, Yingjun Wang
Summary: By evaluating the cell behaviors of bone mesenchymal stem cells (BMSCs) on beta-tricalcium phosphate (β-TCP) scaffolds with different pore morphologies, it was found that the β-TCP scaffold with a diamond pore unit (D-scaffold) enhanced cell cytoskeletal forces, elongated nucleus, increased cell mobility, and promoted osteogenic differentiation potential. The involvement of the Ras homolog gene family A (RhoA)/Rho-associated kinase-2 (ROCK2) signaling pathway in the pore morphology-mediated BMSCs behaviors was revealed, highlighting the importance of mechanical signaling transduction in scaffold-cell interactions. Furthermore, femoral condyle defect repair results demonstrated that D-scaffold effectively promoted endogenous bone regeneration.
BIOACTIVE MATERIALS
(2023)
Review
Cell Biology
Chunfeng Xu, Mingjie Wang, Behrouz Zandieh-Doulabi, Wei Sun, Lingfei Wei, Yuelian Liu
Summary: BMP-2, while potentially useful in repairing bone defects after OS resection, has limited application due to concerns about its tumorigenicity. Studies are focusing on understanding the impact of BMSCs' response to BMP-2 on OS development to pave the way for safer and broader clinical use of BMP-2 in the future.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Feng Zhi, Yi Ding, Rong Wang, Yujiao Yang, Kaiming Luo, Fei Hua
Summary: Exosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis and regulates the balance between osteogenesis and adipogenesis in hBMSCs by sponging miR-431-5p.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biophysics
Zhibiao Bai, Kai Hu, Zeyu Shou, Han Zhou, Jiahuan Yu, Hongming Meng, Liangyan Chen, Tiantian Yu, Ruofei Lu, Na Li, Chun Chen
Summary: This study investigated the effect of PC/Mucin multilayers on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). The results showed that PC/Mucin multilayers promoted the differentiation of BMSCs into osteoblasts and this effect was mediated by the activation of the Wnt/β-catenin pathway. These findings have important implications for the development of materials and coatings for bone regeneration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Multidisciplinary Sciences
Alexandra N. Rindone, Xiaonan Liu, Stephanie Farhat, Alexander Perdomo-Pantoja, Timothy F. Witham, Daniel L. Coutu, Mei Wan, Warren L. Grayson
Summary: Vascularization plays a critical role in cranial bone growth, maintenance, and healing. The study utilized a quantitative imaging platform to analyze the spatial relationship between blood vessels and skeletal progenitors in the murine calvarium, demonstrating specific vessel types are spatially correlated with skeletal progenitors during various cranial processes. Furthermore, the findings suggest a potential role for osteoclasts in maintaining the native cranial microvascular environment.
NATURE COMMUNICATIONS
(2021)