Journal
CELLULAR MICROBIOLOGY
Volume 18, Issue 10, Pages 1415-1428Publisher
WILEY
DOI: 10.1111/cmi.12583
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Funding
- Swiss National Science Foundation [31003A_149297/1]
- Wellcome Trust [088497/Z/09/Z]
- Lundbeck Foundation
- Danish Council for Independent Research, Medical Sciences, Sapere Aude program [DFF-4004-00624B]
- Swiss National Science Foundation (SNF) [31003A_149297] Funding Source: Swiss National Science Foundation (SNF)
- Lundbeck Foundation [R140-2013-13448] Funding Source: researchfish
- BBSRC [BB/J008265/1] Funding Source: UKRI
- MRC [MR/N009274/1] Funding Source: UKRI
- Wellcome Trust [088497/Z/09/Z] Funding Source: Wellcome Trust
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Adherence of Plasmodium falciparum-infected erythrocytes to host endothelium is conferred through the parasite-derived virulence factor P. falciparum erythrocyte membrane protein 1 (PfEMP1), the major contributor to malaria severity. PfEMP1 located at knob structures on the erythrocyte surface is anchored to the cytoskeleton, and the Plasmodium helical interspersed subtelomeric (PHIST) gene family plays a role in many host cell modifications including binding the intracellular domain of PfEMP1. Here, we show that conditional reduction of the PHIST protein PFE1605w strongly reduces adhesion of infected erythrocytes to the endothelial receptor CD36. Adhesion to other endothelial receptors was less affected or even unaltered by PFE1605w depletion, suggesting that PHIST proteins might be optimized for subsets of PfEMP1 variants. PFE1605w does not play a role in PfEMP1 transport, but it directly interacts with both the intracellular segment of PfEMP1 and with cytoskeletal components. This is the first report of a PHIST protein interacting with key molecules of the cytoadherence complex and the host cytoskeleton, and this functional role seems to play an essential role in the pathology of P. falciparum.
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