Article
Biochemistry & Molecular Biology
Xiao Lu, Alice Prodger, Jingwei Sim, Colin E. Evans
Summary: Cancer patients have a higher risk of thrombosis compared to individuals without cancer. Thrombosis can also be a diagnostic predictor of cancer, but the mechanisms by which thrombosis promotes tumor propagation are not fully understood. This study investigates the role of hypoxia-inducible factors (HIF) in myeloid cells in thrombosis-induced tumorigenesis. The results show that HIF1-alpha and HIF2-alpha levels are increased in myeloid cells of murine thrombi. Additionally, thrombosis increases HIF1-alpha and HIF2-alpha levels in murine lungs, promoting tumor development. In vitro studies also demonstrate that hypoxia upregulates tumorigenic factors in the secretome of neutrophils and macrophages, leading to lung cancer cell proliferation and migration in a myeloid-HIF-dependent manner. These findings highlight the importance of thrombotic insult and myeloid HIFs in mediating tumorigenic success.
Review
Cell Biology
Ana Karen Torres-Soria, Yair Romero, Yalbi Balderas-Martinez, Rafael Velazquez-Cruz, Luz Maria Torres-Espindola, Angel Camarena, Edgar Flores-Soto, Hector Solis-Chagoyan, Victor Ruiz, Angeles Carlos-Reyes, Citlaltepetl Salinas-Lara, Erika Rubi Luis-Garcia, Jaime Chavez, Manuel Castillejos-Lopez, Arnoldo Aquino-Galvez
Summary: This review focuses on the impact of hypoxia on the pathophysiology of idiopathic pulmonary fibrosis (IPF), particularly in lung development, regeneration, and repair. The study finds that HIF-1α and HIF-2α have different roles in the acute and chronic phases of hypoxia response, with HIF-2α being involved in persistent aberrant regeneration associated with IPF development.
Article
Oncology
Elodie Jouberton, Aurelien Voissiere, Frederique Penault-Llorca, Florent Cachin, Elisabeth Miot-Noirault
Summary: An increasing number of studies suggest that the interaction between tumor cells and their microenvironment plays a predominant role in the development of solid cancers. In order to mimic microtumors, researchers have designed artificial in vitro 3D culture models, such as spheroids. This project aimed to develop and characterize a multicellular tumor spheroid (MCTS) model of human prostate cancer cells for drug screening. The results showed that this model successfully mimicked a microtumor and was suitable for validating new therapeutic approaches targeting proliferation and the microenvironment.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Review
Microbiology
Wei Liu, Xueni Fan, Boshuo Jian, Dongxu Wen, Hongzhuang Wang, Zhenjiang Liu, Bin Li
Summary: This article mainly discusses the role of hypoxia in maintaining gut homeostasis, gut microbiome, and intestinal barrier functionality, as well as its potential role in the development and treatment of gastrointestinal disorders.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Irina A. Okkelman, Chris Vercruysse, Alina Kondrashina, Sergey M. Borisov, Ruslan Dmitriev
Summary: Multicellular spheroids are important tools for studying tissue and cancer physiology, but the real physiological environment inside the spheroids is generally ignored. Characterization and standardization of spheroid phenotype are required.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Immunology
Olalekan Chris Akinsulie, Sammuel Shahzad, Seto Charles Ogunleye, Ifeoluwa Peace Oladapo, Melina Joshi, Charles Egede Ugwu, Joy Olaoluwa Gbadegoye, Fasilat Oluwakemi Hassan, Richard Adeleke, Qudus Afolabi Akande, Ridwan Olamilekan Adesola
Summary: There are reports on the influence of hypoxia on cancer cells, but there may be more interesting functions of a hypoxic cellular micro-environment. The interaction between HIF and NF-κB is complex and has common factors and targets. This understanding could lead to novel therapeutic targets for infectious diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Qi Wang, Juan Liu, Wenzhen Yin, Dawei Sun, Zhongsong Man, Shangwei Jiang, Xiufeng Ran, Yuxin Su, Yunfang Wang, Jiahong Dong
Summary: This study revealed a strong correlation between FGFR4 and HCC clinicopathological characteristics, and predicted several potential drug candidates using drug-gene related databases. Additionally, the research team developed an efficient agarose hydrogel microwells method for evaluating the anti-tumor effects of these drugs. The results demonstrated that Parthenolide could enhance the clinical efficacy of FGFR4 receptor inhibitors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Samantha M. M. McCabe, Gregory Q. Q. Wallace, Sian Sloan-Dennison, William J. J. Tipping, Neil C. C. Shand, Duncan Graham, Marie Boyd, Karen Faulds
Summary: This study investigates the uptake and distribution of silica coated gold nanoparticles (AuNP-SHINs) in three-dimensional multicellular tumour spheroids derived from glioblastoma cells. The results show that the localization of nanoparticles within the spheroids is influenced by factors such as size, quiescence, and hypoxia.
Article
Biochemical Research Methods
Mai Takagi, Masumi Yamada, Rie Utoh, Minoru Seki
Summary: In this study, a multiscale microfluidic system was developed for the perfusion culture of spheroids, addressing the limitation of diffusion-based oxygen/nutrient transport. The system allows for size-controlled formation of spheroids and direct medium supply, making it suitable for precision cell culture in drug development, disease modeling, and regenerative medicine.
Article
Physiology
Christian Albert Devaux, Didier Raoult
Summary: This article discusses the hypothesis of lower incidence of COVID-19 in high altitude populations and suggests that the molecular mechanism of HIF-1 regulation may be involved, particularly the inhibitory effect of HIF-1α on SARS-CoV-2 infection through ACE2 receptor expression reduction.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yeonhwa Song, Sanghwa Kim, Jinyeong Heo, David Shum, Su-Yeon Lee, Minji Lee, A-Ram Kim, Haeng Ran Seo
Summary: This study identified retinoic acid and forskolin as potential inhibitors of fibrosis by attenuating the compactness of MCTSs and inhibiting the expression of ECM-related proteins. These drugs induced reprogramming of fibroblast and cancer stem cells in the HCC microenvironment, showing anti-fibrosis effects.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Nadege Bidan, Sainza Lores, Aure Vanhecke, Valerie Nicolas, Severine Domenichini, Rafael Lopez, Maria de la Fuente, Simona Mura
Summary: The study found that sphingomyelin nanosystems are promising carriers for efficient delivery of anticancer drugs. A 3D heterotype spheroid model of pancreatic tumor was constructed to investigate the potential of bare and polyethylene glycol modified lipid nanosystems in terms of their ability to penetrate the tumor mass and deliver drugs. The results showed that the lipid nanosystems successfully diffused through the spheroid without inducing toxicity, regardless of their surface properties. However, in the 3D tumor model, despite their efficient penetration capacity, the advantage of the nanosystems was lost when loaded with a lipid prodrug of gemcitabine. The 3D tumor model, being able to mimic the tumor and its microenvironment, provided a more realistic information on the cell sensitivity to treatments. The study highlights the importance of using appropriate 3D tumor models for proper in vitro evaluation of nanomedicine efficacy and their timely optimisation.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Pharmacology & Pharmacy
Sayoni Maitra Roy, Purvi Kishore, Disha Saha, Priyanka Ghosh, Rishav Kar, Sourav Barman, Vishal Agrawal, Abhishek Roy, Ratnadip Deb, Shrestha Sherry Chakraborty, Pousali Bag, Partha Sona Maji, Arnab Basu, Tapas Ghatak, Rupam Mukherjee, Amit Ranjan Maity
Summary: The tumor microenvironment complicates the delivery of anticancer therapeutics, but tumor spheroids offer a promising platform for rapid and cost-effective screening and assessment. The inclusion of 3D in vitro models can improve the efficacy of nanomedicine.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Oncology
Ahlem Zaghmi, Erdem Aybay, Long Jiang, Mingmei Shang, Julia Steinmetz-Spah, Fredrik Wermeling, Per Kogner, Marina Korotkova, Paivi Ostling, Per-Johan Jakobsson, Brinton Seashore-Ludlow, Karin Larsson
Summary: High-throughput drug screening can discover new anticancer drugs, but monolayer cell cultures have limitations, requiring alternative models. Three-dimensional cell cultures, such as multicellular tumor spheroids (MCTS), mimic tumor architecture and offer promising opportunities for drug discovery. In this study, a neuroblastoma MCTS model was developed for high-content drug screening and to decipher the mechanisms underlying synergistic drug combinations in this disease model.
MOLECULAR ONCOLOGY
(2023)
Article
Biology
Ni Li, Xiaofeng Dai, Fei Yang, Yang Sun, Xingwen Wu, Qianrong Zhou, Kai Chen, Jian Sun, Wei Bi, Le Shi, Youcheng Yu
Summary: Spontaneous spheroids from AB-MSCs enhance stemness and pluripotency. HIF-1/2 alpha plays an important role in the stemness regulation of spheroids. AB-MSC spheroids exhibit excellent multi-differentiation capability, which may be a potent therapy for craniomaxillofacial tissue regeneration.
BIOLOGICAL RESEARCH
(2023)