Article
Biochemistry & Molecular Biology
Tatsuhiro Ayabe, Chika Takahashi, Rena Ohya, Yasuhisa Ano
Summary: Beta-lactolin improves mitochondrial functions and prevents neuronal cell death in Alzheimer's disease-related neuronal cell models, demonstrating its advantage in maintaining neuronal health.
Article
Medicine, Research & Experimental
Kunmei Liu, Panpan Wan, Yue Huang, Bin Wang, Xuequan Wang, Rui Zhang, Le Guo
Summary: This study aimed to elucidate the function and mechanism of AEG-1 in neurons by constructing a stable AEG-1-deficient HT22 neuronal cell line. The results revealed that AEG-1 regulates neuronal morphology and development by modulating the expression of several genes. AEG-1 deficiency also impacts multiple signaling pathways involved in cell survival, differentiation, apoptosis, migration, as well as cellular ion homeostasis. These findings provide insights into AEG-1-dependent gene regulation and signaling pathway transduction in neurons, improving our understanding of AEG-1-associated central nervous system diseases.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Biology
John C. O'Donnell, Erin M. Purvis, Kaila V. T. Helm, Dayo O. Adewole, Qunzhou Zhang, Anh D. Le, D. Kacy Cullen
Summary: The Tissue-Engineered Rostral Migratory Stream (TE-RMS) developed by O'Donnell et al. uses astrocyte-like cells derived from adult gingival stem cells to facilitate directed migration of immature neurons and redirect neuroblast migration in athymic rat brains, offering a promising new approach to neuroregenerative medicine.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria Baleva, Ivan Chicherin, Uliana Piunova, Viktor Zgoda, Maxim Patrushev, Sergey Levitskii, Piotr Kamenski
Summary: Protein biosynthesis in mitochondria is closely linked to the assembly of inner membrane complexes and must be coordinated with cytosolic translation. The molecular mechanisms underlying mitochondrial translation regulation are not well understood. This study reveals that PTCD2 functions as a translational regulator of another mitochondrially encoded subunit of cytochrome c oxidase, COIII.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Jiejie Sun, Jingzhou Liu, Chunhong Gao, Jinpeng Zheng, Jinbang Zhang, Yaning Ding, Wei Gong, Meiyan Yang, Zhiping Li, Yuli Wang, Yang Yang, Chunsheng Gao
Summary: In this study, a neuronal mitochondria-specific PARP inhibitor olaparib was developed and red blood cell-coated nanostructured lipid carriers were designed for targeted delivery to brain neuronal mitochondria. The results showed that this approach effectively improved mitochondrial function and prevented neuronal cell death caused by excessive activation of mitochondrial PARP.
ACTA BIOMATERIALIA
(2022)
Article
Cell Biology
Mustafa Guzel, Mustafa Naziroglu, Orhan Akpinar, Ramazan Cinar
Summary: IFNg treatment led to TRPM2 activation in SH-SY5Y cells, resulting in increased cell death and cytokine generation; CRC treatment reduced adverse effects like cell death, cytokine generation, and apoptosis by inhibiting TRPM2 activation.
Article
Biology
Luana Naia, Catarina M. Pinho, Giacomo Dentoni, Jianping Liu, Nuno Santos Leal, Duarte M. S. Ferreira, Bernadette Schreiner, Riccardo Filadi, Ligia Fao, Niamh M. C. Connolly, Pontus Forsell, Gunnar Nordvall, Makoto Shimozawa, Elisa Greotti, Emy Basso, Pierre Theurey, Anna Gioran, Alvin Joselin, Marie Arsenian-Henriksson, Per Nilsson, A. Cristina Rego, Jorge L. Ruas, David Park, Daniele Bano, Paola Pizzo, Jochen H. M. Prehn, Maria Ankarcrona
Summary: This study established a large-scale screening platform for mitochondrial-based modulators and identified luteolin as a compound with potential therapeutic validity for various human diseases by increasing mitochondrial activity through enhancing interactions between mitochondria and endoplasmic reticulum. The findings suggest a novel mechanism for luteolin's modulation of mitochondrial function in neuronal models and potential therapeutic applications.
Article
Multidisciplinary Sciences
Haruhiko Miyata, Seiya Oura, Akane Morohoshi, Keisuke Shimada, Daisuke Mashiko, Yuki Oyama, Yuki Kaneda, Takafumi Matsumura, Ferheen Abbasi, Masahito Ikawa
Summary: SPATA33 interacts with sperm calcineurin via a PQIIIT sequence, and its knockout mice exhibit reduced sperm motility and impaired male fertility, which phenocopies Ppp3cc and Ppp3r2 knockout mice. Further analysis reveals that SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Jovana Drljaca, Aleksandra Popovic, Dragica Bulajic, Nebojsa Stilinovic, Sasenka Vidicevic Novakovic, Slobodan Sekulic, Ivan Milenkovic, Srdan Ninkovic, Marko Ljubkovic, Ivan Capo
Summary: This study investigates the combined effects of TMZ and DIA on GBM cells. The results show that there is antagonism between TMZ and DIA, and DIA decreases the apoptosis level caused by TMZ. Additionally, the concomitant use of TMZ and DIA increases the mitochondrial bioenergetic response and the activities of key complexes in the electron transport chain.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Nadia Turton, Robert A. Heaton, Fahima Ismail, Sioned Roberts, Sian Nelder, Sue Phillips, Iain P. Hargreaves
Summary: The study indicated neuronal cell CoQ10 deficiency and mitochondrial dysfunction following exposure to organophosphates. CoQ10 supplementation was able to ameliorate some deficiencies induced by organophosphates, but had limited effect on the recovery of certain activities.
NEUROCHEMICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Esther Feng Ying Ng, Atsushi Kaida, Hitomi Nojima, Masahiko Miura
Summary: IGFBP-3 enhances cell migration independently of the cell cycle in TSCC and is associated with the MEK/ERK signaling pathway.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Analytical
Daniela Oprea, Caroline G. Sanz, Madalina M. Barsan, Teodor Adrian Enache
Summary: PC-12 cells are widely used in biosensing devices due to their neurogenic characteristics and extensive evaluation in neuroscience research. Optical and electrochemical techniques play a key role in this field.
Article
Biology
Andrea McQuate, Sharmon Knecht, David W. Raible
Summary: Hair cells of the inner ear are highly sensitive to changes in mitochondria, which are essential for energy production in all eukaryotic cells. The mitochondrial phenotype of hair cells, including high volume and specific architecture, has been characterized using zebrafish as a model. Disrupting this mitochondrial phenotype affects mitochondrial health and function, providing insights into mitochondrial deafness and the regulation of hair cell physiology.
Article
Biochemical Research Methods
Anamika Agrawal, Elena F. Koslover
Summary: Neurons rely on localized mitochondria to meet varying spatial demands, with mitochondrial aging occurring faster than the neuronal lifespan. Maintaining optimal mitochondrial distribution involves interactions between stationary and motile pools, with occasional exchange through fusion or replacement events. Infrequent servicing between pools, selective recycling through mitophagy, and robust maintenance via transient fusion are key for mitochondrial homeostasis in neurons, impacting neurological disorders.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Harald Frankowski, Fred Yeboah, Bonnie J. Berry, Chizuru Kinoshita, Michelle Lee, Kira Evitts, Joshua Davis, Yoshito Kinoshita, Richard S. Morrison, Jessica E. Young
Summary: This study reveals that levels of HDAC2 decrease while neuron-specific isoforms of Endophilin-B1 increase during hiPSC differentiation, potentially related to mitochondrial dynamics and neuronal development. Manipulation of HDAC2 and Endophilin-B1 in hiPSC-Ns affects mitochondrial morphology, cytotoxic stress response, and gene expression related to mitochondrial dynamics and synaptogenesis, highlighting the role of HDAC2 in regulating key neuronal and mitochondrial pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kamil Brzoska, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Eva Biela, Martyna Elas, Lucyna Kapka-Skrzypczak, Hanna Lewandowska-Siwkiewicz, Krystyna Urbanska, Marcin Kruszewski
Summary: Gold nanoparticles (AuNPs) were investigated for their potential anticancer effects in tumor growth and metastasis. The results showed that AuNPs did not inhibit tumor growth but caused significant vasodilation in the tumor tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Krzysztof Sawicki, Magdalena Czajka, Magdalena Matysiak-Kucharek, Jacek Kurzepa, Paulina Wojtyla-Buciora, Karol Zygo, Marcin Kruszewski, Lucyna Kapka-Skrzypczak
Summary: This study investigated the impact of the pesticide chlorpyrifos (CPS) on the expression of enzymes involved in synthesis and metabolism of vitamin D3 in human keratinocytes and fibroblasts. The results indicate that exposure to CPS significantly altered the expression of cytochrome P450 isoforms in cells, potentially disrupting vitamin D3 metabolism. Further research is needed to better understand these mechanisms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Oncology
Monika Ruzycka-Ayoush, Patrycja Kowalik, Agata Kowalczyk, Piotr Bujak, Anna M. Nowicka, Maria Wojewodzka, Marcin Kruszewski, Ireneusz P. Grudzinski
Summary: A new targeted drug delivery system based on Ag-In-Zn-S quantum dots was developed for lung cancer treatment. The system demonstrated significant cytotoxicity and genotoxicity effects, showing potential as an effective therapy for lung cancer.
CANCER NANOTECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Aleksandra Paterek, Barbara Sochanowicz, Marta Okninska, Witold Smigielski, Marcin Kruszewski, Urszula Mackiewicz, Michal Maczewski, Przemyslaw Leszek
Summary: Ivabradine may prevent the detrimental effects of dopamine treatment during exacerbations of chronic heart failure, potentially offering additional benefits beyond heart rate reduction. However, preventing ventricular HCN4 overexpression may not play a major role in this setting.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Genetics & Heredity
Somayeh Shahmoradi Ghahe, Konrad Kosicki, Maria Wojewodzka, Bartosz A. Majchrzak, Anna Fogtman, Roksana Iwanicka-Nowicka, Agata Ciuba, Marta Koblowska, Marcin Kruszewski, Barbara Tudek, Elzbieta Speina
Summary: Resistance to photodynamic therapy in cancer cells may be caused by various mechanisms, including efficient repair of oxidative DNA damage and DNA breaks. Higher activity of APE1 endonuclease and increased expression and activation of DNA damage kinase ATM in the U-87 MGR cell line suggest that they are potential targets for sensitizing resistant cells to PDT.
Article
Nutrition & Dietetics
Monika Kalinowska, Ewelina Golebiewska, Grzegorz Swiderski, Sylwia Meczynska-Wielgosz, Hanna Lewandowska, Anna Pietryczuk, Adam Cudowski, Aleksander Astel, Renata Swislocka, Mariola Samsonowicz, Anna Barbara Zlowodzka, Waldemar Priebe, Wlodzimierz Lewandowski
Summary: The study investigated seven derivatives of plant-derived hydroxybenzoic acid for their structural and biological properties, including antioxidant, lipophilicity, antimicrobial activity, and cytotoxicity. It discussed the relationship between the molecular structure and biological activity of hydroxybenzoic acids, providing valuable information for their potential application in dietary supplements, functional foods, or drugs.
Article
Biochemistry & Molecular Biology
Katarzyna Dziendzikowska, Jacek Wilczak, Wojciech Grodzicki, Joanna Gromadzka-Ostrowska, Malgorzata Wesierska, Marcin Kruszewski
Summary: The study found that silver nanoparticles disrupted the antioxidant system in the hippocampus of rats and induced oxidative stress in a coating-dependent manner, which could potentially lead to neurodegeneration and cognitive disorders. The coating material of the nanoparticles also modulated the concentrations of steroids and the expression of genes involved in steroid synthesis and metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Bogna Kozlowska, Barbara Sochanowicz, Leszek Kraj, Malgorzata Palusinska, Piotr Kolsut, Lukasz Szymanski, Slawomir Lewicki, Witold Smigielski, Marcin Kruszewski, Przemyslaw Leszek
Summary: This study investigated the changes in iron-related proteins in the myocardium of heart failure patients. The results showed reduced expression of several proteins responsible for iron transport and storage, along with increased expression of oxidative stress markers. The reduction of iron-gathering proteins and elevated oxidative stress in failing hearts is detrimental to myocardial cells.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Physical
Kamil Brzoska, Maria Wojewodzka, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Eva Biela, Martyna Elas, Malgorzata Kucinska, Hanna Piotrowska-Kempisty, Lucyna Kapka-Skrzypczak, Marek Murias, Krystyna Urbanska, Marcin Kruszewski
Summary: This study analyzed the impact of different silver nanoparticles on the development and metastatic potential of breast cancer using a mouse model. The results showed that citrate-coated silver nanoparticles had a significant effect on reducing tumor volume and lung metastases in mice treated intragastrically. However, no effect on lung metastases was observed after intravenous administration of silver nanoparticles. The findings suggest that the anticancer activity of silver nanoparticles may depend on the activation of the immune system.
Article
Biochemistry & Molecular Biology
Michal Oczkowski, Jacek Wilczak, Katarzyna Dziendzikowska, Johan Ovrevik, Oddvar Myhre, Anna Lankoff, Marcin Kruszewski, Joanna Gromadzka-Ostrowska
Summary: This study investigated the effects of exposure to biodiesel exhaust emissions on oxidative stress in testes using an animal model. The results showed that dietary intervention with blackcurrant pomace could reduce oxidative stress in testes to some extent.
Review
Biology
Bogna Kozlowska, Barbara Sochanowicz, Leszek Kraj, Malgorzata Palusinska, Piotr Kolsut, Lukasz Szymanski, Slawomir Lewicki, Marcin Kruszewski, Marta Zaleska-Kociecka, Przemyslaw Leszek
Summary: Heart failure (HF) is a common disease and iron deficiency (ID) plays a significant role in worsening the condition of HF patients. Iron supplementation is recommended but may have adverse effects. There are still many knowledge gaps regarding the interaction between iron deficiency and HF. Summarizing the latest translational and clinical research on iron deficiency in HF is important for raising awareness among clinicians.
Article
Engineering, Environmental
Iwona Bartosiewicz, Bogdan Szostek, Tomasz Szreder, Anna Bojanowska-Czajka, Sylwia Meczynska-Wielgosz, Stanislaw Chmiel, Krzysztof Bobrowski, Marek Trojanowicz
Summary: The decomposition of methylparaben (MP) under oxidative conditions was investigated using gamma-radiation in aerated aqueous solutions. Stable products derived from MP were identified, and the irradiation of aerated MP solutions was found to be more efficient in terms of MP decomposition.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Anna Lankoff, Malwina Czerwinska, Marcin Kruszewski
Summary: Prostate cancer is a common and deadly malignancy in men, with limited treatment options for advanced stages. Nanoparticles have been developed to selectively target cancer cells and improve imaging and therapy. This review focuses on the selection criteria and progress of nanoparticle-based radioconjugates for targeted imaging and therapy of prostate cancer.
Article
Biochemistry & Molecular Biology
Magdalena Czajka, Krzysztof Sawicki, Magdalena Matysiak-Kucharek, Marcin Kruszewski, Jacek Kurzepa, Paulina Wojtyla-Buciora, Lucyna Kapka-Skrzypczak
Summary: Chlorpyrifos, a representative of organophosphorus pesticides, has effects on the viability, proliferation, differentiation, and fatty acid uptake in 3T3-L1 cells. Exposure to chlorpyrifos decreases cell viability but increases it at low concentrations. It inhibits proliferation and differentiation of preadipocytes, reduces lipid accumulation, and down-regulates key transcription factors. In fully differentiated adipocytes, chlorpyrifos increases basal free fatty acid uptake. These findings suggest that chlorpyrifos may play a role in the development of obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Physical
Katarzyna Sikorska, Krzysztof Sawicki, Magdalena Czajka, Lucyna Kapka-Skrzypczak, Marcin Kruszewski, Kamil Brzoska
Summary: The interest in nanoparticles and their effects on living organisms has been increasing in recent decades. Non-metallic nanoparticles appear to be less toxic than metallic ones, leading to their growing use in medicine and industry. This review summarizes current knowledge of the in vitro and in vivo neurotoxicity of non-metallic nanoparticles, as well as the mechanisms associated with their positive or negative effects on the central nervous system (CNS).