Review
Oncology
Raymond S. S. Traweek, Brandon M. M. Cope, Christina L. L. Roland, Emily Z. Z. Keung, Elise F. F. Nassif, Derek J. J. Erstad
Summary: Dedifferentiated liposarcoma (DDLPS) is a aggressive adipogenic cancer with poor prognosis. DDLPS is characterized by low tumor mutational burden and frequent chromosomal structural abnormalities, including amplification of the 12q13-15 chromosomal region and the MDM2 gene. MDM2, an E3 ubiquitin ligase, plays a role in cell cycle progression and has been targeted as a therapeutic option for DDLPS. Multiple agents have been developed, but early-phase clinical trials have not shown a benefit. Research is ongoing to elucidate resistance mechanisms and explore novel inhibitors and combination therapies.
FRONTIERS IN ONCOLOGY
(2022)
Review
Medicine, General & Internal
Mariana Deacu, Mariana Aschie, Madalina Bosoteanu, Sorin Vamesu, Gabriela-Izabela Baltatescu, Georgeta Camelia Cozaru, Cristian Ionut Orasanu, Raluca Ioana Voda
Summary: This case report describes a young patient initially diagnosed with left hydrocele, but after 2 years, it was revealed to be a differentiated liposarcoma. Pathological examination and immunohistochemical and genetic testing confirmed the diagnosis. The patient ultimately died from acute renal failure. This case highlights the importance of proper management of oncological patients and the role of immunohistochemical and genetic testing in identifying prognostic factors.
Article
Oncology
Kyle D. Klingbeil, Jack Pengfei Tang, Danielle S. Graham, Serena Y. Lofftus, Amit Kumar Jaiswal, Tasha L. Lin, Chris Frias, Lucia Y. Chen, Manando Nakasaki, Sarah M. Dry, Joseph G. Crompton, Fritz C. Eilber, Dinesh S. Rao, Anusha Kalbasi, Brian E. Kadera
Summary: IGF2BP3 is highly expressed in well-differentiated/dedifferentiated liposarcoma and is associated with poorer survival and disease progression.
Review
Biochemistry & Molecular Biology
Jun Lu, David Wood, Evan Ingley, Sulev Koks, Daniel Wong
Summary: Well-differentiated liposarcoma (WDLPS) can transform into dedifferentiated liposarcoma (DDLPS), which is more aggressive. Retroperitoneal lesions of WDLPS/DDLPS tend to recur due to incomplete resections, and current treatments have limited effectiveness. Therefore, identifying therapeutic targets and developing targeted drugs is crucial.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Multidisciplinary Sciences
Joanna Przybyl, Lien Spans, Kristen Ganjoo, Nam Bui, David Mohler, Jeffrey Norton, George Poultsides, Maria Debiec-Rychter, Matt van de Rijn
Summary: This study found that amplification of MDM2 and other genes in plasma cell-free DNA may be used for distinguishing liposarcomas and other retroperitoneal tumors as well as for early detection of disease recurrence. Tracking MDM2 amplification in cell-free DNA may also be useful for evaluating response to treatment. The results of this study have clinical implications for improved diagnosis and surveillance of patients with retroperitoneal tumors.
Article
Oncology
Kyoko Yamashita, Kenichi Kohashi, Yuichi Yamada, Shinya Akatsuka, Kunihiro Ikuta, Yoshihiro Nishida, Shinya Toyokuni, Yoshinao Oda
Summary: This study investigated the progression process and factors related to poor prognosis in DDLPS, finding that MDM2 and HMGA2 genes were associated with poor and good prognosis, respectively. The amplification or gain ratio of MDM2/HMGA2 was determined to have significant prognostic value in patients with DDLPS.
GENES CHROMOSOMES & CANCER
(2021)
Article
Oncology
Tao Rui, Xiaobing Zhang, Jufeng Guo, Aizhai Xiang, Ning Tang, Jian Liu, Zonglei Mao
Summary: This study analyzed the small RNA species in serum exosomes and found that miRNAs were the most important components. Three serum-exosome-derived miRNAs (miR-122-5p, let-7d-5p, and miR-425-5p) were found to have significant diagnostic values for hepatocellular carcinoma (HCC). The results suggest that serum-exosome-derived miRNAs could be promising biomarkers for HCC diagnosis.
Article
Medicine, General & Internal
William G. Baker, Shayla Albright, Tiffany Aragan, Raul Mederos
Summary: This article reports a case of dedifferentiated gastric liposarcoma, which is the largest ever recorded. The patient initially received multiple biopsies that indicated a benign cause, but the diagnosis was only confirmed after surgical resection. The article discusses histopathology, genetic markers, and differential diagnoses.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Xinyue Zhang, Ruling Yang, Mengjie Ma, Siyu Chen, Kaiyin Guo, Li Zhang, Yingnan You, Hailin Jia, Bo Han
Summary: This study investigated the plasma exosome miRNA profile in pediatric fulminant myocarditis (FM) using small RNA microarray. Differential expression of 266 miRNAs was observed, including 197 upregulated and 69 downregulated candidate genes. Through analysis, FM-specific target genes related to inflammation and/or the immune system were identified. CeRNA networks between cardiomyocytes and peripheral blood mononuclear cells were established, and specific miRNAs were validated as potential biomarkers for FM diagnosis.
Article
Medical Laboratory Technology
Xinlei Zou, Ziyue Huang, Canghai Guan, Wujiang Shi, Jianjun Gao, Jiangang Wang, Yunfu Cui, Mei Wang, Yi Xu, Xiangyu Zhong
Summary: Pancreatic cancer (PC) is highly aggressive with a poor prognosis. The tumor microenvironment (TME) of PC is complex and diverse. Various cellular components in the microenvironment secrete different substances that promote tumor development. These substances can be released via exosomes, which are abundant extracellular vesicles (EVs) capable of carrying factors and enabling intercellular communication. MiRNAs are involved in regulating pathological and physiological processes and can be transported by exosomes to regulate the TME. Exosomal miRNAs hold promise as future targets for PC diagnosis and prognosis, offering potential new treatment strategies.
CLINICA CHIMICA ACTA
(2023)
Review
Oncology
Giuliana Cassinelli, Sandro Pasquali, Cinzia Lanzi
Summary: Well differentiated and dedifferentiated liposarcomas are poorly responsive to conventional chemotherapy, highlighting the need for new therapeutic targets. Amplification of MDM2 and CDK4 driver genes provide a rationale for targeted therapy and other potential targets have been identified. Studies on molecular characteristics and genetic alterations hope to improve understanding and identification of tailored treatments.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Jian Cui, Ran Peng, Yahan Zhang, Yang Lu, Xin He, Min Chen, Hongying Zhang
Summary: This case report describes a rare primary bladder liposarcoma in a 69-year-old Chinese woman. The tumor was dedifferentiated with low-grade dedifferentiation. After surgical resection, the patient remained disease-free during long-term follow-up.
FRONTIERS IN ONCOLOGY
(2023)
Review
Biology
Patricia Petrouskova, Nikola Hudakova, Marcela Maloveska, Filip Humenik, Dasa Cizkova
Summary: This review summarizes the research progress on miRNAs as biomarkers of canine mammary cancer, including miRNAs from non-exosomal sources and from exosomes. Some of the miRNAs may be considered potential disease-specific biomarkers in both CMC and HBC.
Review
Oncology
L. Zhu, L. Zhao, Q. Wang, S. Zhong, X. Guo, Y. Zhu, J. Bao, K. Xu, S. Liu
Summary: miRNAs are small non-coding ribonucleic acids that play key roles in cancer development and can be encapsulated in exosomes released into the circulatory system. Circulating exosome-derived miRNAs have the potential to replace conventional biomarkers for early cancer diagnosis.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2022)
Article
Pathology
Olga Kuczkiewicz-Siemion, Piotr Wisniewski, Agnieszka Dansonka-Mieszkowska, Magdalena Grabowska-Kieryl, Katarzyna Olszewska, Tomasz Goryn, Monika Prochorec-Sobieszek, Piotr Rutkowski, Anna Szumera-Cieckiewicz
Summary: Amplification of the MDM2 and DDIT3 genes was observed in DDLPS with MLPS-like morphology. However, the association between DDIT3 amplification and MLPS-like features reported in previous studies was not confirmed. Therefore, evaluation of DDIT3 status may be crucial in accurately classifying DDLPS.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Oncology
Danielle Braggio, David Koller, Feng Jin, Nanda Siva, Abeba Zewdu, Gonzalo Lopez, Kara Batte, Lucia Casadei, Meng Welliver, Anne M. Strohecker, Dina Lev, Raphael E. Pollock
Article
Oncology
John H. Alexander, Sumanas W. Jordan, Julie M. West, Amy Compston, Jennifer Fugitt, J. Byers Bowen, Gregory A. Dumanian, Raphael Pollock, Joel L. Mayerson, Thomas J. Scharschmidt, Ian L. Valerio
JOURNAL OF SURGICAL ONCOLOGY
(2019)
Article
Oncology
Lucia Casadei, Federica Calore, Danielle A. Braggio, Abeba Zewdu, Ameya A. Deshmukh, Paolo Fadda, Gonzalo Lopez, Martin Wabitsch, Chi Song, Jennifer L. Leight, Valerie P. Grignol, Dina Lev, Carlo M. Croce, Raphael E. Pollock
Article
Surgery
Malcolm H. Squires, Cecilia G. Ethun, Lorena P. Suarez-Kelly, Peter Y. Yu, Tasha M. Hughes, Rita D. Shelby, Thuy B. Tran, George Poultsides, John Charlson, T. Clark Gamblin, Jennifer Tseng, Kevin K. Roggin, Konstantinos Chouliaras, Konstantinos Votanopoulos, Bradley A. Krasnick, Ryan C. Fields, Raphael E. Pollock, Valerie Grignol, Kenneth Cardona, J. Harrison Howard
JOURNAL OF SURGICAL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Danielle Braggio, Abeba Zewdu, Priya Londhe, Peter Yu, Gonzalo Lopez, Kara Batte, David Koller, Fernanda Costas Casal de Faria, Lucia Casadei, Anne M. Strohecker, Dina Lev, Raphael E. Pollock
Review
Oncology
Lucia Casadei, Raphael E. Pollock
Article
Engineering, Electrical & Electronic
Prashanth Mohana Sundaram, Lucia Casadei, Gonzalo Lopez, Danielle Braggio, Gita Balakirsky, Raphael Pollock, Shaurya Prakash
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
(2020)
Article
Cell Biology
Lucia Casadei, Adarsh Choudhury, Patricia Sarchet, Prashanth Mohana Sundaram, Gonzalo Lopez, Danielle Braggio, Gita Balakirsky, Raphael Pollock, Shaurya Prakash
Summary: This study presents a resource-efficient approach using a micro-nanofluidic device for isolating and capturing liposarcoma-derived extracellular vesicles, leading to a significant advance in retrieval of critical liposarcoma-relevant extracellular vesicle cargo compared to state-of-the-art methods.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Oncology
Soumya R. Sharma, Nadia E. Paonessa, Lucia Casadei, Fernanda Costas De Faria, Raphael E. Pollock, Valerie Grignol
Summary: Soft tissue sarcomas are a heterogeneous group of tumors that arise from mesenchymal tissue, with recent research providing new insight into potential biomarkers at the genomic, proteomic, and immunological levels. The identification of biomarkers can improve diagnosis, prognosis, prediction of recurrence, and treatment response in STS.
JOURNAL OF SURGICAL ONCOLOGY
(2022)
Review
Biology
Anita Adib, Ruhi Sahu, Shivangi Mohta, Raphael Etomar Pollock, Lucia Casadei
Summary: This review investigates the role of extracellular vesicles (EVs) in sarcoma, exploring how these vesicles can affect the phenotype of recipient cells and the aggressiveness of the tumor. Studying EVs in sarcoma may lead to the development of novel therapeutic approaches targeting specific sarcoma subtypes or biologies.
Review
Oncology
Yu Wang, Farhan Z. Ilyas, Mahsa Kheradmandi, Diamantis I. Tsilimigras, Valerie P. Grignol, Carlo Contreras, Philip N. Tsichlis, Raphael E. Pollock, Joal D. Beane
Summary: Soft tissue sarcomas (STS) are a biologically diverse and poor prognosis group of mesenchymal tumors. Surgical resection is the main treatment method, but some patients are not eligible for surgery and there is a high rate of tumor recurrence in operated patients. Conventional systemic therapies are not effective. Aberrant activation of the AKT pathway is found in many cancers, including STS. Despite decades of research on EGFRs and its downstream effectors PI3K and AKT/mTOR, targeting the AKT pathway for advanced sarcoma remains challenging with no improvement in prognosis.
MOLECULAR CANCER RESEARCH
(2022)
Article
Oncology
Alessandro La Ferlita, Nipin Sp, Marina Goryunova, Giovanni Nigita, Raphael E. Pollock, Carlo M. Croce, Joal D. Beane
Summary: Soft-tissue sarcomas (STS), a rare and diverse group of tumors, have various subtypes characterized by differences in tumor biology and clinical outcomes. Small non-coding RNAs (sncRNAs), including miRNAs and tRNA-derived ncRNAs, play important roles in STS tumorigenesis and differentiation. However, the translation of these findings into clinical applications, such as biomarkers or therapeutic targets, remains challenging. This review summarizes the current understanding of sncRNAs in STS, with a focus on liposarcoma, and discusses their potential as novel biomarkers and therapeutic targets.
MOLECULAR CANCER RESEARCH
(2023)
Article
Cell Biology
Sayumi Tahara, Fernanda Costas C. de Faria, Patricia Sarchet, Federica Calore, Joe Sharick, Jennifer L. Leight, Lucia Casadei, Raphael E. Pollock
Summary: This study aimed to establish 3D cell culture of liposarcomas using two different methods: scaffold-based (Matrigel extracellular matrix [ECM] scaffold method) and scaffold-free (Ultra-low attachment [ULA] plate). The distinctive differences in structures and different responses of cell lines to 3D culture were observed. The upregulation of MDM2 was confirmed after treatment with MDM2 inhibitor SAR405838, and DDLPS spheroids showed different sensitivity patterns from 2D models. The 3D models have the potential to provide a new predictability of efficacy and toxicity, and are considered as an important process in the pre-clinical phase.
Review
Oncology
Samantha M. Ruff, Valerie P. Grignol, Carlo M. Contreras, Raphael E. Pollock, Joal D. Beane
Summary: Retroperitoneal sarcoma (RPS) is a rare disease with various histologic types. The Transatlantic RPS Working Group (TARPSWG) in Europe and North America aims to study and establish best practices for managing this disease. Complete resection of all macro and microscopic disease is the gold standard, but it often requires multi-visceral resection. Recent evidence suggests en bloc compartmental resections can reduce local recurrence, but the potential for added morbidity must be considered.
