Article
Veterinary Sciences
Patricia Marini, Luciana Fernandez Beato, Fernando Cane, Juan Manuel Teijeiro
Summary: The addition of 2 mM ZnCl2 to diluted boar sperm did not affect sperm viability, motility, mitochondrial activity, DNA integrity or ROS levels. However, ZnCl2 improved acrosomal integrity and sperm kinematics.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Reproductive Biology
Guo Chen, Li Ren, Zhanglin Chang, Yuting Zhao, Yanwen Zhang, Dong Xia, Ruqian Zhao, Bin He
Summary: High glucose concentration-induced lysine acetylation participates in the regulation of boar spermatozoa motility and acrosome integrity during preservation.
Article
Reproductive Biology
Pawel Brym, Karolina Wasilewska-Sakowska, Marzena Mogielnicka-Brzozowska, Anna Mankowska, Lukasz Paukszto, Chandra S. Pareek, Wladyslaw Kordan, Stanislaw Kondracki, Leyland Fraser
Summary: This study identified polymorphic variants in the 5'-flanking regulatory regions of genes expressed in boar spermatozoa, which were found to be associated with semen freezability. These variants can influence the interactions of transcription factors with gene promoters, thereby affecting gene expression levels.
Article
Agriculture, Dairy & Animal Science
Francisco Sevilla, Carles Soler, Ignacio Araya-Zuniga, Vinicio Barquero, Eduardo R. S. Roldan, Anthony Valverde
Summary: This study examined the accuracy of four methods in assessing boar sperm concentration and motility. The results showed differences among these methods, with iSperm(R) method performing better in concentration estimation. Further research is needed to better understand these differences.
Article
Reproductive Biology
Ana Carolina Pedrosa, Mariana Andrade Torres, Diego Vilela Alkmin, Jorge E. P. Pinzon, Simone Maria Massami Kitamura Martins, Juliano Coelho da Silveira, Andre Furugen Cesar de Andrade
Summary: Research has shown that ssc-miR-503, ssc-miR-130a, and ssc-miR-9 are related to low sperm cryotolerance in boar semen and can be used as biomarkers to predict their low ability to tolerate the cryopreservation process.
Article
Chemistry, Analytical
Stella A. Kruit, Douwe S. de Bruijn, Marleen L. W. J. Broekhuijse, Wouter Olthuis, Loes Segerink
Summary: Microfluidics and lab-on-chip technologies can serve as versatile and efficient alternatives in semen analysis and fertility treatments in the veterinary industry and human fertility clinics.
Article
Cell Biology
Min Zhang, Riccardo Zenezini Chiozzi, David A. Skerrett-Byrne, Tineke Veenendaal, Judith Klumperman, Albert J. R. Heck, Brett Nixon, J. Bernd Helms, Bart M. Gadella, Elizabeth G. Bromfield
Summary: This study provides in-depth characterization of the perinuclear theca (PT) of boar spermatozoa through subcellular fractionation, revealing 1802 identified proteins. The PT was found to be enriched in proteins involved in sperm-egg fusion and egg activation, as well as endoplasmic reticulum (ER) proteins and the ER-stress response. Protein-protein interaction analysis visualized an intricate network of PT protein complexes, including proteasome subunits.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Yongjie Xu, Qiu Han, Chaofeng Ma, Yaling Wang, Pengpeng Zhang, Cencen Li, Xiaofang Cheng, Haixia Xu
Summary: Comprehensive proteomics analysis of sperm from Yorkshire and Duroc boars revealed differential expression of proteins and phosphorylated proteins related to crucial physiological functions such as sperm development, motility, and energy metabolism. Proteins involved in axonemal dynein complex assembly and sperm-egg recognition were found to be significantly higher in Duroc spermatozoa, indicating potential differences in sperm function between the two boar breeds.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Andrology
S. L. Soares, Camila R. C. Brito, Andreia Nobre Anciuti, Norton C. Gatti, Carine Dahl Corcini, Antonio Sergio Jr Varela Jr, Mariana G. Marques, Francisco N. Fonseca, Eliza R. Komninou, Thomaz Jr Lucia Jr
Summary: This study showed that adding 40 μg/ml nanoencapsulated resveratrol can improve post-thawing kinetics of boar spermatozoa, while inclusion of vitamin E does not significantly improve sperm quality. Increasing the concentration of antioxidants and utilizing nanoencapsulated forms may be an effective method to reduce oxidative stress injuries in boar sperm during freezing.
Article
Veterinary Sciences
Josue Calderon-Calderon, Francisco Sevilla, Eduardo R. S. Roldan, Vinicio Barquero, Anthony Valverde
Summary: This study aimed to evaluate the effect of fat-soluble vitamin supplementation on the seminal quality of boars. The results showed that fat-soluble vitamin supplementation increased semen volume, motility, progressive motility, and velocity variables. Overall, dietary fat-soluble vitamin supplementation significantly improved the semen quality of boar ejaculates.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Martin Kadlec, Eliana Pintus, Jose Luis Ros-Santaella
Summary: Recent experiments have shown the important role of nitric oxide (NO) and hydrogen sulfide (H2S) in male gametes. Both gasotransmitters can affect sperm structure and function, as well as the impact of oxidative stress. A study on boar spermatozoa found that the combination of NO and H2S had a positive effect on progressive motility and plasma membrane integrity. The combination of the two gasotransmitters was more effective than single-donor application.
Article
Veterinary Sciences
Yanbing Li, Hechuan Wang, Shuo Wang, Qun Zhang, Han Zhang, Tianfeng Li, Qian Wang, Minghui Guo, Heze Feng, Yulun Song, Guosheng Wei, Jingchun Li
Summary: After artificial insemination, the presence of immune cells in the genital tract can cause inflammation and negatively affect sperm quality. This study investigated the effects of methylprednisolone on preserving boar sperm quality during in vitro liquid preservation and on the chemotaxis and phagocytosis of immune cells towards sperm. The results showed that a specific concentration of methylprednisolone improved sperm motility, antioxidant capacity, and inhibited immune cell activity towards sperm.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Ondrej Simonik, Filipa Bubenickova, Lucie Tumova, Michaela Frolikova, Vishma Pratap Sur, Jan Beran, Katerina Havlikova, Lenka Hackerova, Daniela Spevakova, Katerina Komrskova, Pavla Postlerova
Summary: This study evaluated the effect of biocompatible polysaccharides, dextran and pentaisomaltose, on the cryopreservation of boar sperm and used computational modeling to predict their interaction with sperm surface proteins. The results showed that the modified extender with pentaisomaltose had a lower impact on sperm quality parameters. Pentaisomaltose represented a promising compound for the elimination or reduction of glycerol in extenders for boar sperm cryopreservation.
Article
Veterinary Sciences
Bin Zhang, Yan Wang, Caihong Wu, Shulei Qiu, Xiaolan Chen, Bingyan Cai, Huimei Xie
Summary: In this study, the quality of boar spermatozoa at different steps of cryopreservation was evaluated, revealing that excessive ROS during freeze-thaw cycling can negatively affect sperm motility, plasma membrane integrity, mitochondrial activity, sperm chromatin structure, and apoptosis. Addition of the antioxidant NAC reversed these negative effects, highlighting the importance of ROS downregulation for successful frozen pig sperm production and livestock propagation.
BMC VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Heiko Henning, Quynh Thu Nguyen, Ulrike Wallner, Dagmar Waberski
Summary: The optimum storage temperature for liquid-preserved boar semen is between 17 and 25 degrees C to maintain stable sperm quality and energy status. Temperatures below 10 degrees C can cause cold shock and imbalance in available ATP in viable spermatozoa, affecting sperm quality and motility. Further measures are needed to address this issue for hypothermic boar semen preservation below 17 degrees C.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biology
Charles A. Elder, Jensen S. Smith, Mustafa Almosawi, Ethan Mills, Brett R. Janis, Jonathan A. Kopechek, Willem F. Wolkers, Michael A. Menze
Summary: By using a biomimetics approach and trehalose as a cryoprotective agent, red blood cells can be successfully preserved for long-term storage without the need for time-consuming washing steps.