The emerging PFOS alternative OBS exposure induced gut microbiota dysbiosis and hepatic metabolism disorder in adult zebrafish

Sodium rho-perfluorous nonenoxybenzene sulfonate (OBS), as a novel the alternatives of PFASs, is widely used in many fields of life. Here, adult male zebrafish selected were exposed to OBS at concentrations of 3, 30 and 300 mu g/L for 7 and 21 days, respectively. Based on the gut microbiota analysis, at genus level, the relative abundance of the Flavobacterium, Hyphomicrobium, Paracoccus, Lawsonia, Plesiomonas and Vibrio changed significantly in the gut of zebrafish after exposure to 300 mu g/L OBS. In addition, the liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis suggested that a total of 1077 metabolites in pos-model and a total of 706 metabolites in neg-model changed significantly from the liver, and these changed metabolites were tightly related to several pathways including amino acid, pyrimidine and purine metabolism, etc. Furthermore, the changed gut bacteria including Flavobacterium, Hyphomicrobium, Paracoccus, Lawsonia, Plesiomonas and Vibrio at genus level were significantly correlated with various metabolites (succinic acid, leucine, xanthine, orotic acid, nicotinic acid, etc.). Taken together, all the results showed that low dose of OBS exposure could induce the dysbiosis of gut microbiota and disturbed the hepatic metabolism balance in adult male zebrafish.